Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicos
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFPB |
Texto Completo: | https://repositorio.ufpb.br/jspui/handle/123456789/14867 |
Resumo: | Depression is a serious and disabling psychiatric disorder with a high incidence in the population. The treatment involves drugs that possesses undesired effects and in many cases, decreases the patient's adherence to the treatment. This fact has encouraged the search for new drugs and the vegetable kingdom has been widely investigated in this sense. Flavonoids are an important class of secondary metabolites found in different plant species. Quercetin (3, 5, 7, 3 '- 4' pentahydroxy flavone),is one of the most abundant flavonoids in the human diet, it has has several biological activities such as: anti-inflammatory, anti-aggregating, antihypertensive, diabetic, anxiolytic and antidepressant. This last activity, however, is still not well characterized and this fact led to the development of the present study, which aimed to evaluate the antidepressant-like effect of quercetin, through behavioral and neurochemical tests. Male Swiss mice (CEUA / UFPB protocol 112/2016) were used, from the Animal Production Unit of the Federal University of Paraíba - UPA / UFPB. The animals were divided into groups composed of six mice, which were orally treated with saline, quercetin 10 and 40 mg / kg and intraperitoneally with imipramine 30 mg / kg and subjected to open field and forced swimming tests. For the purpose of investigating the possible mechanism of action, quercetin was administered alone (0.5 mg / kg, v) and in combination with antidepressants bupropion (0.1 mg / kg, ip), fluoxetine (0.1 mg / kg , ip) and imipramine (1 mg / kg, ip) in animals submitted to forced swimming. In addition, neurochemical analyzes were performed to determine the striatal concentration of monoamines and the hippocampal concentration of nitrite and TBARs. The results showed that the administration of quercetin did not alter the locomotor activity of the animals submitted to the open field; on the other hand, the compound in question significantly and dose-dependently reduced the immobility time of animals in the forced swimming. This effect was potentiated in the presence of bupropion and imipramine, but not of fluoxetine. Treatment with quercetin (10 mg / kg, v.o.) did not change the hippocampal concentration of nitrite and TBARs, two important markers of oxidative stress. Finally, oral administration of flavonoid significantly increased the concentration of noradrenaline, but not dopamine, in the striatum of animals subjected to the forced swimming test. Taken together, the results suggest that the antidepressant-like effect of quercetin seems to depend, at least in part, on the increase in the striatal concentration of monoamines, especially noradrenaline. |
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Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicosDepressãoNado forçadoFlavonóidesQuercetinaMonoaminasEstresse oxidativoDepressionForced swim testFlavonoidsQuercetinMonoaminesOxidative stressCNPQ::CIENCIAS HUMANAS::PSICOLOGIADepression is a serious and disabling psychiatric disorder with a high incidence in the population. The treatment involves drugs that possesses undesired effects and in many cases, decreases the patient's adherence to the treatment. This fact has encouraged the search for new drugs and the vegetable kingdom has been widely investigated in this sense. Flavonoids are an important class of secondary metabolites found in different plant species. Quercetin (3, 5, 7, 3 '- 4' pentahydroxy flavone),is one of the most abundant flavonoids in the human diet, it has has several biological activities such as: anti-inflammatory, anti-aggregating, antihypertensive, diabetic, anxiolytic and antidepressant. This last activity, however, is still not well characterized and this fact led to the development of the present study, which aimed to evaluate the antidepressant-like effect of quercetin, through behavioral and neurochemical tests. Male Swiss mice (CEUA / UFPB protocol 112/2016) were used, from the Animal Production Unit of the Federal University of Paraíba - UPA / UFPB. The animals were divided into groups composed of six mice, which were orally treated with saline, quercetin 10 and 40 mg / kg and intraperitoneally with imipramine 30 mg / kg and subjected to open field and forced swimming tests. For the purpose of investigating the possible mechanism of action, quercetin was administered alone (0.5 mg / kg, v) and in combination with antidepressants bupropion (0.1 mg / kg, ip), fluoxetine (0.1 mg / kg , ip) and imipramine (1 mg / kg, ip) in animals submitted to forced swimming. In addition, neurochemical analyzes were performed to determine the striatal concentration of monoamines and the hippocampal concentration of nitrite and TBARs. The results showed that the administration of quercetin did not alter the locomotor activity of the animals submitted to the open field; on the other hand, the compound in question significantly and dose-dependently reduced the immobility time of animals in the forced swimming. This effect was potentiated in the presence of bupropion and imipramine, but not of fluoxetine. Treatment with quercetin (10 mg / kg, v.o.) did not change the hippocampal concentration of nitrite and TBARs, two important markers of oxidative stress. Finally, oral administration of flavonoid significantly increased the concentration of noradrenaline, but not dopamine, in the striatum of animals subjected to the forced swimming test. Taken together, the results suggest that the antidepressant-like effect of quercetin seems to depend, at least in part, on the increase in the striatal concentration of monoamines, especially noradrenaline.NenhumaA depressão é um transtorno psiquiátrico com alta incidência na população. O tratamento envolve fármacos que possuem uma série de efeitos adversos que, em muitos casos, diminuem a adesão do paciente ao tratamento. Isto tem incentivado a busca por novos fármacos e o reino vegetal se apresenta como uma alternativa. Os flavonoides são uma classe de metabólitos secundários encontrados em diferentes espécies de plantas. A quercetina (3, 5, 7, 3’ – 4’ pentahidroxi flavona), um importante flavonoide, possui diversas atividades biológicas, entre elas: atividade anti-inflamatória, anti-agregante, anti-hipertensiva, antidiabética, ansiolítica e antidepressiva. Esta última atividade, no entanto, ainda não é bem caracterizada e este fato levou ao desenvolvimento do presente trabalho, o qual teve como objetivo avaliar o efeito antidepressivo-símile da quercetina, por meio de testes comportamentais e neuroquímicos. Foram utilizados camundongos Swiss machos (protocolo CEUA/UFPB n. 112/2016), provenientes da Unidade de Produção Animal da Universidade Federal da Paraíba – UPA/UFPB. Os animais foram divididos em grupos compostos por seis camundongos, tratados por via oral com salina, quercetina 10 ou 40 mg/kg e por via intraperitoneal com imipramina 30 mg/kg, e submetidos aos testes do campo aberto e nado forçado. Para investigação do possível mecanismo de ação, a quercetina foi administrada isoladamente (0,5 mg/kg, v.o.) e em associação aos antidepressivos bupropiona (0,1 mg/kg, i.p.), fluoxetina (0,1 mg/kg, i.p.) ou imipramina (1 mg/kg, i.p.) em animais submetidos ao nado forçado. Em adição foram realizadas análises neuroquímicas para determinar a concentração estriatal de monoaminas e a concentração hipocampal de nitrito e TBARs. Os resultados mostraram que a quercetina não alterou a atividade locomotora no teste do campo aberto; por outro lado, o composto reduziu de forma significativa e dose-dependente o tempo de imobilidade dos animais no nado forçado. Este efeito foi potencializado na presença de bupropiona e imipramina, mas não de fluoxetina. A quercetina (10 mg/kg, v.o.) não alterou a concentração hipocampal de nitrito e TBARs; por outro lado, o composto aumentou significativamente a concentração de noradrenalina, mas não de dopamina, no estriado de animais submetidos ao teste do nado forçado. Tomados em conjunto, os resultados sugerem que o efeito antidepressivo-símile da quercetina parece depender, pelo menos em parte, do aumento da concentração estriatal de monoaminas, em especial, a noradrenalina.Universidade Federal da ParaíbaBrasilPsicologia SocialPrograma de Pós-Graduação em Neurociência Cognitiva e ComportamentoUFPBFelipe, Cícero Francisco Bezerrahttp://lattes.cnpq.br/7960322400408876Carvalho, Bernardo Hollanda de2019-06-27T19:00:37Z2019-06-272019-06-27T19:00:37Z2018-09-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttps://repositorio.ufpb.br/jspui/handle/123456789/14867porAttribution-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2019-06-27T19:00:37Zoai:repositorio.ufpb.br:123456789/14867Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2019-06-27T19:00:37Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false |
dc.title.none.fl_str_mv |
Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicos |
title |
Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicos |
spellingShingle |
Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicos Carvalho, Bernardo Hollanda de Depressão Nado forçado Flavonóides Quercetina Monoaminas Estresse oxidativo Depression Forced swim test Flavonoids Quercetin Monoamines Oxidative stress CNPQ::CIENCIAS HUMANAS::PSICOLOGIA |
title_short |
Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicos |
title_full |
Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicos |
title_fullStr |
Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicos |
title_full_unstemmed |
Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicos |
title_sort |
Investigação do mecanismo de ação antidepressivo-símile da quercetina em camundongos por meio de testes comportamentais e neuroquímicos |
author |
Carvalho, Bernardo Hollanda de |
author_facet |
Carvalho, Bernardo Hollanda de |
author_role |
author |
dc.contributor.none.fl_str_mv |
Felipe, Cícero Francisco Bezerra http://lattes.cnpq.br/7960322400408876 |
dc.contributor.author.fl_str_mv |
Carvalho, Bernardo Hollanda de |
dc.subject.por.fl_str_mv |
Depressão Nado forçado Flavonóides Quercetina Monoaminas Estresse oxidativo Depression Forced swim test Flavonoids Quercetin Monoamines Oxidative stress CNPQ::CIENCIAS HUMANAS::PSICOLOGIA |
topic |
Depressão Nado forçado Flavonóides Quercetina Monoaminas Estresse oxidativo Depression Forced swim test Flavonoids Quercetin Monoamines Oxidative stress CNPQ::CIENCIAS HUMANAS::PSICOLOGIA |
description |
Depression is a serious and disabling psychiatric disorder with a high incidence in the population. The treatment involves drugs that possesses undesired effects and in many cases, decreases the patient's adherence to the treatment. This fact has encouraged the search for new drugs and the vegetable kingdom has been widely investigated in this sense. Flavonoids are an important class of secondary metabolites found in different plant species. Quercetin (3, 5, 7, 3 '- 4' pentahydroxy flavone),is one of the most abundant flavonoids in the human diet, it has has several biological activities such as: anti-inflammatory, anti-aggregating, antihypertensive, diabetic, anxiolytic and antidepressant. This last activity, however, is still not well characterized and this fact led to the development of the present study, which aimed to evaluate the antidepressant-like effect of quercetin, through behavioral and neurochemical tests. Male Swiss mice (CEUA / UFPB protocol 112/2016) were used, from the Animal Production Unit of the Federal University of Paraíba - UPA / UFPB. The animals were divided into groups composed of six mice, which were orally treated with saline, quercetin 10 and 40 mg / kg and intraperitoneally with imipramine 30 mg / kg and subjected to open field and forced swimming tests. For the purpose of investigating the possible mechanism of action, quercetin was administered alone (0.5 mg / kg, v) and in combination with antidepressants bupropion (0.1 mg / kg, ip), fluoxetine (0.1 mg / kg , ip) and imipramine (1 mg / kg, ip) in animals submitted to forced swimming. In addition, neurochemical analyzes were performed to determine the striatal concentration of monoamines and the hippocampal concentration of nitrite and TBARs. The results showed that the administration of quercetin did not alter the locomotor activity of the animals submitted to the open field; on the other hand, the compound in question significantly and dose-dependently reduced the immobility time of animals in the forced swimming. This effect was potentiated in the presence of bupropion and imipramine, but not of fluoxetine. Treatment with quercetin (10 mg / kg, v.o.) did not change the hippocampal concentration of nitrite and TBARs, two important markers of oxidative stress. Finally, oral administration of flavonoid significantly increased the concentration of noradrenaline, but not dopamine, in the striatum of animals subjected to the forced swimming test. Taken together, the results suggest that the antidepressant-like effect of quercetin seems to depend, at least in part, on the increase in the striatal concentration of monoamines, especially noradrenaline. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-26 2019-06-27T19:00:37Z 2019-06-27 2019-06-27T19:00:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufpb.br/jspui/handle/123456789/14867 |
url |
https://repositorio.ufpb.br/jspui/handle/123456789/14867 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
Attribution-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Attribution-NoDerivs 3.0 Brazil http://creativecommons.org/licenses/by-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Psicologia Social Programa de Pós-Graduação em Neurociência Cognitiva e Comportamento UFPB |
publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Psicologia Social Programa de Pós-Graduação em Neurociência Cognitiva e Comportamento UFPB |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFPB instname:Universidade Federal da Paraíba (UFPB) instacron:UFPB |
instname_str |
Universidade Federal da Paraíba (UFPB) |
instacron_str |
UFPB |
institution |
UFPB |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFPB |
collection |
Biblioteca Digital de Teses e Dissertações da UFPB |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB) |
repository.mail.fl_str_mv |
diretoria@ufpb.br|| diretoria@ufpb.br |
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1801842950456475648 |