Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiro
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFPB |
Texto Completo: | https://repositorio.ufpb.br/jspui/handle/123456789/19311 |
Resumo: | Surfactants are amphipathic molecules capable of interacting with surfaces of different polarities and because they are derived from petroleum, they have limitations and problems associated with their use, among which environmental ones stand out. Biosurfactants are an alternative because they demonstrate environmentally desirable characteristics that depend on the metabolism of organisms, associated with technologies that favor their production. In this context, we highlight the use of sugarcane molasses as an alternative source of substrate. Therefore, the objective of this work was to produce biosurfactant in bench top bioreactor using Bacillus safensis, MF326565, isolated from sugarcane soil and sugarcane molasses as an alternative substrate. The experiments were carried out preliminarily in shaker (1% of sugarcane molasses and 1% of yeast extract - w / v). The diagnosis of biosurfactant production was made by growth analysis, surface tension and emulsification index. In bioreactor, they followed the same analyzes, besides the characterization by FTIR and evaluation of antimicrobial activity. The results of shaker cultivation were quite satisfactory regarding growth and biosurfactant production, since the microorganism was able to use the substrate and produce biosurfactant, which presented emulsification index higher than 50% in motor oil, besides presenting reduction. surface tension by 32%. In bioreactor a growth of approximately 3.0 g / l of biomass and 1.5 g / l of biosurfactant was observed and with much higher emulsification in motor oil, vegetable oil and kerosene. The biosurfactant showed stability at temperature (15 ~ 60 ° C), pH (2.0 ~ 8.0), salinity (6 ~ 10% NaCl), and emulsification remained stable for more than 15 days. Infrared spectrograms (FTIR) of the purified product from the bioreactor experiments showed similarity with the surfactin standard, showing its lipopeptide nature. The biosurfactant showed antimicrobial activity against S. aureus, B. cereus, B. safensis, B. subtilis, C. albicans and Acinetobacter sp., bactericidal activity at concentrations of 12.5 mg / mL for Acinetobacter sp. and S. aureus and concentrations below 3.0 mg / mL for Bacillus used in this study. Thus, it is concluded that Bacillus safensis, MF326565, was able to produce biosurfactant from an alternative substrate (molasses from sugarcane) and that it was effective in emulsifying hydrophobic compounds, besides presenting biological activity |
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Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiroBiossurfactanteBacillus safensisResíduos agroindustriaisEstabilidadeAntimicrobianoBiosurfactantAgroindustrial wasteStabilityAntimicrobialCNPQ::CIENCIAS BIOLOGICASSurfactants are amphipathic molecules capable of interacting with surfaces of different polarities and because they are derived from petroleum, they have limitations and problems associated with their use, among which environmental ones stand out. Biosurfactants are an alternative because they demonstrate environmentally desirable characteristics that depend on the metabolism of organisms, associated with technologies that favor their production. In this context, we highlight the use of sugarcane molasses as an alternative source of substrate. Therefore, the objective of this work was to produce biosurfactant in bench top bioreactor using Bacillus safensis, MF326565, isolated from sugarcane soil and sugarcane molasses as an alternative substrate. The experiments were carried out preliminarily in shaker (1% of sugarcane molasses and 1% of yeast extract - w / v). The diagnosis of biosurfactant production was made by growth analysis, surface tension and emulsification index. In bioreactor, they followed the same analyzes, besides the characterization by FTIR and evaluation of antimicrobial activity. The results of shaker cultivation were quite satisfactory regarding growth and biosurfactant production, since the microorganism was able to use the substrate and produce biosurfactant, which presented emulsification index higher than 50% in motor oil, besides presenting reduction. surface tension by 32%. In bioreactor a growth of approximately 3.0 g / l of biomass and 1.5 g / l of biosurfactant was observed and with much higher emulsification in motor oil, vegetable oil and kerosene. The biosurfactant showed stability at temperature (15 ~ 60 ° C), pH (2.0 ~ 8.0), salinity (6 ~ 10% NaCl), and emulsification remained stable for more than 15 days. Infrared spectrograms (FTIR) of the purified product from the bioreactor experiments showed similarity with the surfactin standard, showing its lipopeptide nature. The biosurfactant showed antimicrobial activity against S. aureus, B. cereus, B. safensis, B. subtilis, C. albicans and Acinetobacter sp., bactericidal activity at concentrations of 12.5 mg / mL for Acinetobacter sp. and S. aureus and concentrations below 3.0 mg / mL for Bacillus used in this study. Thus, it is concluded that Bacillus safensis, MF326565, was able to produce biosurfactant from an alternative substrate (molasses from sugarcane) and that it was effective in emulsifying hydrophobic compounds, besides presenting biological activityConselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESOs surfactantes são moléculas anfipáticas capazes de interagir com superfícies de diferentes polaridades e por serem derivados do petróleo, apresentam limitações e problemas associados ao seu uso, dentre estes, destacam-se os ambientais. Os biossurfactantes apresentam-se como alternativa pois demonstram características ambientalmente desejáveis que dependem do metabolismo dos organismos, associado a tecnologias que favoreçam sua produção. Neste contexto, destaca-se o uso do melaço de cana-de-açúcar como fonte alternativa de substrato. Diante deste exposto, o objetivo deste trabalho foi produzir biossurfactante em biorreator de bancada utilizando Bacillus safensis, MF326565, isolado de solo canavieiro e melaço de canade- açúcar como substrato alternativo. Os experimentos foram conduzidos preliminarmente em shaker (1 % de melaço de cana-de-açúcar e 1 % de extrato de levedura – p/v). O diagnóstico da produção de biossurfactante foi realizado por meio da análise de crescimento, tensão superficial e índice de emulsificação. Em biorreator, seguiram as mesmas análises, além da caracterização por FTIR e avaliação da atividade antimicrobiana. Os resultados do cultivo em shaker foram bastante satisfatórios quanto ao crescimento e a produção de biossurfactante, pois o microrganismo foi capaz de utilizar o substrato e produzir biossurfactante, que apresentou índice de emulsificação superior a 50 % em óleo de motor, além de apresentar redução da tensão superficial em 32 %. Em biorreator observou-se um crescimento de aproximadamente 3,0 g/L de biomassa e 1,5 g/L de biossurfactante e com emulsificação muito superior em óleo de motor, óleo vegetal e querosene. O biossurfactante apresentou estabilidade a variação de temperatura (15~60 °C), pH (2,0~8,0), salinidade (6~10 % de NaCl), além disso, a emulsificação permaneceu estável por mais de 15 dias. Os espectrogramas de infravermelho (FTIR) do produto purificado dos experimentos em biorreator apresentaram similaridade com o padrão surfactina, evidenciando sua natureza lipopeptídica. O biossurfactante apresentou atividade antimicrobiana contra S. aureus, B. cereus, B. safensis, B. subtilis, C. albicans e Acinetobacter sp.com atividade bactericida em concentrações de 12,5 mg/mL para Acinetobacter sp. e S. aureus e concentrações menores que 3,0 mg/mL para os Bacillus utilizados neste estudo. Assim, conclui-se que o Bacillus safensis, MF326565, foi capaz de produzir biossurfactante a partir de um substrato alternativo (melaço de cana-de-açúcar) e que este apresentou eficácia na emulsificação de compostos hidrofóbicos, além de apresentar atividade biológicaUniversidade Federal da ParaíbaBrasilBiotecnologiaPrograma de Pós-Graduação em BiotecnologiaUFPBAraújo, Demetrius Antonio Machado dehttp://lattes.cnpq.br/4795833304329411Silva Filho, Ubiratan Ribeiro da2021-02-07T23:53:03Z2020-04-302021-02-07T23:53:03Z2019-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttps://repositorio.ufpb.br/jspui/handle/123456789/19311porhttp://creativecommons.org/licenses/by-nd/3.0/br/info:eu-repo/semantics/embargoedAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2021-08-16T14:31:50Zoai:repositorio.ufpb.br:123456789/19311Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2021-08-16T14:31:50Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false |
dc.title.none.fl_str_mv |
Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiro |
title |
Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiro |
spellingShingle |
Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiro Silva Filho, Ubiratan Ribeiro da Biossurfactante Bacillus safensis Resíduos agroindustriais Estabilidade Antimicrobiano Biosurfactant Agroindustrial waste Stability Antimicrobial CNPQ::CIENCIAS BIOLOGICAS |
title_short |
Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiro |
title_full |
Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiro |
title_fullStr |
Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiro |
title_full_unstemmed |
Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiro |
title_sort |
Produção, extração, purificação e potencial atividade antimicrobiana do biossurfactante produzido por Bacillus safensis MF326565 isolado de solo canavieiro |
author |
Silva Filho, Ubiratan Ribeiro da |
author_facet |
Silva Filho, Ubiratan Ribeiro da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Araújo, Demetrius Antonio Machado de http://lattes.cnpq.br/4795833304329411 |
dc.contributor.author.fl_str_mv |
Silva Filho, Ubiratan Ribeiro da |
dc.subject.por.fl_str_mv |
Biossurfactante Bacillus safensis Resíduos agroindustriais Estabilidade Antimicrobiano Biosurfactant Agroindustrial waste Stability Antimicrobial CNPQ::CIENCIAS BIOLOGICAS |
topic |
Biossurfactante Bacillus safensis Resíduos agroindustriais Estabilidade Antimicrobiano Biosurfactant Agroindustrial waste Stability Antimicrobial CNPQ::CIENCIAS BIOLOGICAS |
description |
Surfactants are amphipathic molecules capable of interacting with surfaces of different polarities and because they are derived from petroleum, they have limitations and problems associated with their use, among which environmental ones stand out. Biosurfactants are an alternative because they demonstrate environmentally desirable characteristics that depend on the metabolism of organisms, associated with technologies that favor their production. In this context, we highlight the use of sugarcane molasses as an alternative source of substrate. Therefore, the objective of this work was to produce biosurfactant in bench top bioreactor using Bacillus safensis, MF326565, isolated from sugarcane soil and sugarcane molasses as an alternative substrate. The experiments were carried out preliminarily in shaker (1% of sugarcane molasses and 1% of yeast extract - w / v). The diagnosis of biosurfactant production was made by growth analysis, surface tension and emulsification index. In bioreactor, they followed the same analyzes, besides the characterization by FTIR and evaluation of antimicrobial activity. The results of shaker cultivation were quite satisfactory regarding growth and biosurfactant production, since the microorganism was able to use the substrate and produce biosurfactant, which presented emulsification index higher than 50% in motor oil, besides presenting reduction. surface tension by 32%. In bioreactor a growth of approximately 3.0 g / l of biomass and 1.5 g / l of biosurfactant was observed and with much higher emulsification in motor oil, vegetable oil and kerosene. The biosurfactant showed stability at temperature (15 ~ 60 ° C), pH (2.0 ~ 8.0), salinity (6 ~ 10% NaCl), and emulsification remained stable for more than 15 days. Infrared spectrograms (FTIR) of the purified product from the bioreactor experiments showed similarity with the surfactin standard, showing its lipopeptide nature. The biosurfactant showed antimicrobial activity against S. aureus, B. cereus, B. safensis, B. subtilis, C. albicans and Acinetobacter sp., bactericidal activity at concentrations of 12.5 mg / mL for Acinetobacter sp. and S. aureus and concentrations below 3.0 mg / mL for Bacillus used in this study. Thus, it is concluded that Bacillus safensis, MF326565, was able to produce biosurfactant from an alternative substrate (molasses from sugarcane) and that it was effective in emulsifying hydrophobic compounds, besides presenting biological activity |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-04-30 2020-04-30 2021-02-07T23:53:03Z 2021-02-07T23:53:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufpb.br/jspui/handle/123456789/19311 |
url |
https://repositorio.ufpb.br/jspui/handle/123456789/19311 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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http://creativecommons.org/licenses/by-nd/3.0/br/ info:eu-repo/semantics/embargoedAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nd/3.0/br/ |
eu_rights_str_mv |
embargoedAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Biotecnologia Programa de Pós-Graduação em Biotecnologia UFPB |
publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Biotecnologia Programa de Pós-Graduação em Biotecnologia UFPB |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFPB instname:Universidade Federal da Paraíba (UFPB) instacron:UFPB |
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Universidade Federal da Paraíba (UFPB) |
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UFPB |
institution |
UFPB |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFPB |
collection |
Biblioteca Digital de Teses e Dissertações da UFPB |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB) |
repository.mail.fl_str_mv |
diretoria@ufpb.br|| diretoria@ufpb.br |
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1801843025877401600 |