Perfil de resistência antimicrobiana em Staphylococcus spp. de origem láctea na região nordeste

• Autor(a) principal: Silva Júnior, Abimael Estevam da
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/15862
• topic: Leite – Infecção intramamária; Staphylococcus spp. – Resistência antimicrobiana; Multirresistência; CIM; CNPQ::CIENCIAS AGRARIAS::ZOOTECNIA

The intramammary infection is considered a disease that causes great economic losses to dairy industry due to discarded milk, drug costs and technical assistance, reducing the quantity and quality of milk and its derivatives. Moreover, it presents serious problem to public health when conveys resistant microorganisms to human population. Due to lack of information about these microorganisms it is difficult to assess the impact of contaminated food on public health. To that end, this paper aimed to investigate the antimicrobial resistance patterns in Staphylococcus spp. grown from samples of cow and goat milk and goat cheese, as well as determine the minimum inhibitory concentration (MIC) for the tested antimicrobials. A total of 549 isolates of Staphylococcus spp. were analyzed, among those, 195 were coagulase- positive and 354 were coagulase-negative, which were from different states of the Northeast. Antimicrobial resistance was evaluated by broth microdilution method using a semi- automated system (Autoscan4® Siemens) determining the MIC for the antimicrobial agents in the following dilutions: amoxicillin/clavulanate [Aug] (2.4 μg/ml), gentamicin [Gen] (4 - 8 μg/ml), clindamycin [Cli] (0.5 – 4 μg/ml), Ciprofloxacin [Cip] (1 - 2 μg/ml), erythromycin [Eri] (0.5 - 4 μg/ml), oxacillin [Oxa] (0,25 – 2 μg/ml), penicillin G [Pen] (0,03 - 8 μg/ml), linezolid [Lin] (1 - 4 μg/ml), trimethoprim/sulfamethoxazole [T/S] (0,5/9,5 - 2/38 μg/ml), tetracycline (4 – 8 mg/mL) and vancomycin [Van] (0,25- 16 μg/ml). To evaluate possible associations between antimicrobial resistance and the independent variables we used chi- square test with 5% significance level. The most frequent species were S. epidermidis 80/354 (22.6%) among the SCN, whereas S. aureus was the most common specie of SCP 122/195 (62.6%) and the most isolated specie among the others. The highest resistance rates were observed for, PEN (38.62%), ERY (22.22%) and TET (18.58%). However the antimicrobial Pen, Tet, Oxa, Cli, Eri, Lin and Van had significant difference (P <0.05) when compared their frequencies between the SCN and SCP groups, obtaining a higher percentage of resistance to SCN. Multidrug resistance was observed in 21/195 SCP and in 71/354 SCN. Among Staphylococcus isolates, 291 showed resistance profile while 258 were pan-susceptible to certain studied antibiotics. The study generated a total of 51 resistance profiles with a greater prevalence for profiles: Pen (12.75%), Pen/Tet (7.83%), Pen/Mac (5.65%), and Pen/Lin/Mac/Oxa/Cli (2.37%). Thus this study provides grants on the resistance profile to X antimicrobials from the minimum inhibitory concentrations, thereby contributing to the treatment