Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substrato
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UFPB |
Texto Completo: | https://repositorio.ufpb.br/jspui/handle/123456789/18290 |
Resumo: | The search for alternative sources of biocomposites with potential for application in several sectors with the ability to accumulate carotenoids and lipids inside their cells, have been extensively studied. The aim of this study was to evaluate the potential of sisal fiber and sisal pulp hydrolysates as substrates for the production of lipids and carotenoids by the yeast Rhodotorula mucilaginosa. The yeast cells were inoculated into the fiber hydrolyzate and sisal pulp, the cultures were incubated in an orbital shaker at 30 °C and 200 rpm. During the 120 h interval, biomass and reducing sugars were quantified. In order to determine the variables that most influence the cultivation of the yeast Rhodotorula mucilaginosa, using sisal bagasse hydrolyzate as a substrate, a Plackett-Burman planning was carried out with 8 input variables: KH2PO4 (g/L), MgSO4 (g/L), temperature (°C), initial pH, yeast extract (g/L), CaCl2 (g/L), (NH4) SO4 (g/L) and NaCl (g/L) and 3 response variables : biomass (g/L), specific production of carotenoids (μg/g) and volumetric production of carotenoids (g/L), totaling 15 assays. Were produced 4.98 g/L of biomass, 19.28 g/100 g of lipids and 0.48 mg/L of carotenoids, using the sisal fiber hydrolyzate as a substrate. The yeast carotenoid production was mainly influenced by temperature and pH, with maximum biomass obtained at 34.0 ºC and pH 5.0, and maximum carotenoid synthesis at 22.0 ºC and pH of 7.0. The yeast grew and accumulated carotenoids and lipids using fiber and sisal pulp hydrolyzate as substrates, producing as maximum values 4.98 g/L of biomass, 19.28% of lipids and 0.49 mg/L of carotenoids, using fiber and 12.87 g/L of biomass, 0.54 g of lipids/L of substrate and 1.13 g/L of carotenoids, using sisal pulp. In the fatty acid profile of the oil obtained, there was a predominance of unsaturated, mainly oleic and linoleic. The hydrolyzates of fiber and sisal pulp proved to be an adequate means for the production of lipids and carotenoids by the yeast Rhodotorula mucilaginosa. The lignocellulosic materials studied have potential to generate hydrolyzates that can be used as a substrate for the cultivation of Rhodotorula mucilaginosa yeast, aiming at obtaining biocomposites, allowing added value to the residue (sisal pulp) and fiber, in addition to minimizing the environmental impacts caused due to improper disposal, with potential for application in the food, chemical, pharmaceutical and cosmetics sectors. |
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Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substratoFibra do sisalBagaço do sisalLevedura oleaginosaLipídiosCarotenoidesSisal fiberSisal pulpOleaginous yeastLipidsCarotenoidsCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSThe search for alternative sources of biocomposites with potential for application in several sectors with the ability to accumulate carotenoids and lipids inside their cells, have been extensively studied. The aim of this study was to evaluate the potential of sisal fiber and sisal pulp hydrolysates as substrates for the production of lipids and carotenoids by the yeast Rhodotorula mucilaginosa. The yeast cells were inoculated into the fiber hydrolyzate and sisal pulp, the cultures were incubated in an orbital shaker at 30 °C and 200 rpm. During the 120 h interval, biomass and reducing sugars were quantified. In order to determine the variables that most influence the cultivation of the yeast Rhodotorula mucilaginosa, using sisal bagasse hydrolyzate as a substrate, a Plackett-Burman planning was carried out with 8 input variables: KH2PO4 (g/L), MgSO4 (g/L), temperature (°C), initial pH, yeast extract (g/L), CaCl2 (g/L), (NH4) SO4 (g/L) and NaCl (g/L) and 3 response variables : biomass (g/L), specific production of carotenoids (μg/g) and volumetric production of carotenoids (g/L), totaling 15 assays. Were produced 4.98 g/L of biomass, 19.28 g/100 g of lipids and 0.48 mg/L of carotenoids, using the sisal fiber hydrolyzate as a substrate. The yeast carotenoid production was mainly influenced by temperature and pH, with maximum biomass obtained at 34.0 ºC and pH 5.0, and maximum carotenoid synthesis at 22.0 ºC and pH of 7.0. The yeast grew and accumulated carotenoids and lipids using fiber and sisal pulp hydrolyzate as substrates, producing as maximum values 4.98 g/L of biomass, 19.28% of lipids and 0.49 mg/L of carotenoids, using fiber and 12.87 g/L of biomass, 0.54 g of lipids/L of substrate and 1.13 g/L of carotenoids, using sisal pulp. In the fatty acid profile of the oil obtained, there was a predominance of unsaturated, mainly oleic and linoleic. The hydrolyzates of fiber and sisal pulp proved to be an adequate means for the production of lipids and carotenoids by the yeast Rhodotorula mucilaginosa. The lignocellulosic materials studied have potential to generate hydrolyzates that can be used as a substrate for the cultivation of Rhodotorula mucilaginosa yeast, aiming at obtaining biocomposites, allowing added value to the residue (sisal pulp) and fiber, in addition to minimizing the environmental impacts caused due to improper disposal, with potential for application in the food, chemical, pharmaceutical and cosmetics sectors.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESA busca por fontes alternativas de biocompostos com potencial para aplicação em diversos setores da indústria tem sido intensa nos últimos anos. Em vista disso, leveduras oleaginosas com a capacidade de acumular carotenoides e lipídios no interior de suas células, têm sido bastante estudadas. Objetivou-se nesse estudo avaliar o potencial dos hidrolisados da fibra e do bagaço do sisal como substratos para a produção de lipídios e carotenoides pela levedura Rhodotorula mucilaginosa. As células de levedura foram inoculadas no hidrolisado de fibra e do bagaço do sisal, as culturas foram incubadas em um agitador orbital a 30 °C e 200 rpm. Durante o intervalo de 120 h, foram quantificados a biomassa e os açúcares redutores. Para a determinação das variáveis que mais influenciam no cultivo da levedura Rhodotorula mucilaginosa, utilizando-se o hidrolisado do bagaço do sisal como substrato, foi realizado um planejamento Plackett-Burman com oito variáveis de entrada: KH2PO4 (g/L), MgSO4 (g/L), temperatura (°C), pH inicial, extrato de levedura (g/L), CaCl2 (g/L), (NH4)SO4 (g/L) e NaCl (g/L) e três variáveis respostas: biomassa (g/L), produção específica de carotenoides (μg/g) e produção volumétrica de carotenoides (g/L), totalizando quinze experimentos. Foram produzidos 4,98 g/L de biomassa, 19,28 g/100 g de lipídios e 0,48 mg/L de carotenoides, utilizando-se o hidrolisado da fibra como substrato. A produção de carotenoides pela levedura mostrou-se influenciada principalmente pela temperatura e pelo pH, sendo a biomassa máxima obtida em temperatura de 34,0 ºC e pH de 5,0, e a síntese de carotenoides máxima em temperatura de 22,0 ºC e pH de 7,0. A levedura cresceu e acumulou carotenoides e lipídios utilizando-se o hidrolisado da fibra e do bagaço do sisal como substratos, sendo produzidos, como valores máximos, 4,98 g/L de biomassa, 19,28% de lipídios e 0,49 mg/L de carotenoides, usando-se a fibra e 12,87 g/L de biomassa, 0,54 g de lipídios/L de substrato e 1,13 g/L de carotenoides, utilizando-se o bagaço. No perfil de ácidos graxos do óleo obtido houve predominância de insaturados, principalmente oleico e linoleico. Os hidrolisados da fibra e do bagaço de sisal demonstraram ser meios adequados para a produção de lipídios e carotenoides pela levedura Rhodotorula mucilaginosa. Os materiais lignocelulósicos estudados apresentam potencial para geração de hidrolisados que podem ser usados como substrato para o cultivo da levedura Rhodotorula mucilaginosa, visando à obtenção de biocompostos, possibilitando agregação de valor ao resíduo (bagaço) e à fibra, além da minimização dos impactos ambientais provocados pelo descarte inadequado, com potencial para aplicação no setor alimentício, químico, farmacêutico e de cosméticos.Universidade Federal da ParaíbaBrasilEngenharia de AlimentosPrograma de Pós-Graduação em Ciência e Tecnologia de AlimentosUFPBSilva, Flávio Luiz Honorato dahttp://lattes.cnpq.br/2082780006180637Silva, Josevan da2020-10-26T18:27:30Z2020-05-222020-10-26T18:27:30Z2020-03-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttps://repositorio.ufpb.br/jspui/handle/123456789/18290porhttp://creativecommons.org/licenses/by-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2021-09-15T14:07:54Zoai:repositorio.ufpb.br:123456789/18290Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2021-09-15T14:07:54Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false |
dc.title.none.fl_str_mv |
Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substrato |
title |
Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substrato |
spellingShingle |
Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substrato Silva, Josevan da Fibra do sisal Bagaço do sisal Levedura oleaginosa Lipídios Carotenoides Sisal fiber Sisal pulp Oleaginous yeast Lipids Carotenoids CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
title_short |
Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substrato |
title_full |
Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substrato |
title_fullStr |
Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substrato |
title_full_unstemmed |
Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substrato |
title_sort |
Produção de biocompostos por Rhodotorula mucilaginosa utilizando o hidrolisado do sisal (Agave sisalana) como substrato |
author |
Silva, Josevan da |
author_facet |
Silva, Josevan da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Silva, Flávio Luiz Honorato da http://lattes.cnpq.br/2082780006180637 |
dc.contributor.author.fl_str_mv |
Silva, Josevan da |
dc.subject.por.fl_str_mv |
Fibra do sisal Bagaço do sisal Levedura oleaginosa Lipídios Carotenoides Sisal fiber Sisal pulp Oleaginous yeast Lipids Carotenoids CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
topic |
Fibra do sisal Bagaço do sisal Levedura oleaginosa Lipídios Carotenoides Sisal fiber Sisal pulp Oleaginous yeast Lipids Carotenoids CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
description |
The search for alternative sources of biocomposites with potential for application in several sectors with the ability to accumulate carotenoids and lipids inside their cells, have been extensively studied. The aim of this study was to evaluate the potential of sisal fiber and sisal pulp hydrolysates as substrates for the production of lipids and carotenoids by the yeast Rhodotorula mucilaginosa. The yeast cells were inoculated into the fiber hydrolyzate and sisal pulp, the cultures were incubated in an orbital shaker at 30 °C and 200 rpm. During the 120 h interval, biomass and reducing sugars were quantified. In order to determine the variables that most influence the cultivation of the yeast Rhodotorula mucilaginosa, using sisal bagasse hydrolyzate as a substrate, a Plackett-Burman planning was carried out with 8 input variables: KH2PO4 (g/L), MgSO4 (g/L), temperature (°C), initial pH, yeast extract (g/L), CaCl2 (g/L), (NH4) SO4 (g/L) and NaCl (g/L) and 3 response variables : biomass (g/L), specific production of carotenoids (μg/g) and volumetric production of carotenoids (g/L), totaling 15 assays. Were produced 4.98 g/L of biomass, 19.28 g/100 g of lipids and 0.48 mg/L of carotenoids, using the sisal fiber hydrolyzate as a substrate. The yeast carotenoid production was mainly influenced by temperature and pH, with maximum biomass obtained at 34.0 ºC and pH 5.0, and maximum carotenoid synthesis at 22.0 ºC and pH of 7.0. The yeast grew and accumulated carotenoids and lipids using fiber and sisal pulp hydrolyzate as substrates, producing as maximum values 4.98 g/L of biomass, 19.28% of lipids and 0.49 mg/L of carotenoids, using fiber and 12.87 g/L of biomass, 0.54 g of lipids/L of substrate and 1.13 g/L of carotenoids, using sisal pulp. In the fatty acid profile of the oil obtained, there was a predominance of unsaturated, mainly oleic and linoleic. The hydrolyzates of fiber and sisal pulp proved to be an adequate means for the production of lipids and carotenoids by the yeast Rhodotorula mucilaginosa. The lignocellulosic materials studied have potential to generate hydrolyzates that can be used as a substrate for the cultivation of Rhodotorula mucilaginosa yeast, aiming at obtaining biocomposites, allowing added value to the residue (sisal pulp) and fiber, in addition to minimizing the environmental impacts caused due to improper disposal, with potential for application in the food, chemical, pharmaceutical and cosmetics sectors. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-10-26T18:27:30Z 2020-05-22 2020-10-26T18:27:30Z 2020-03-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufpb.br/jspui/handle/123456789/18290 |
url |
https://repositorio.ufpb.br/jspui/handle/123456789/18290 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nd/3.0/br/ info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nd/3.0/br/ |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Engenharia de Alimentos Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos UFPB |
publisher.none.fl_str_mv |
Universidade Federal da Paraíba Brasil Engenharia de Alimentos Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos UFPB |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UFPB instname:Universidade Federal da Paraíba (UFPB) instacron:UFPB |
instname_str |
Universidade Federal da Paraíba (UFPB) |
instacron_str |
UFPB |
institution |
UFPB |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UFPB |
collection |
Biblioteca Digital de Teses e Dissertações da UFPB |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB) |
repository.mail.fl_str_mv |
diretoria@ufpb.br|| diretoria@ufpb.br |
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1801842960615079936 |