Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipira

Detalhes bibliográficos
Autor(a) principal: Cunha, Rebeka Correia de Souza
Data de Publicação: 2022
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/24206
Resumo: One of the most important by-products of chicken slaughter, bone residues, are an excellent source of protein and can therefore be used as protein hydrolysate obtained by enzymatic hydrolysis. This technique has allowed the use of meat by-products with a wide technological application, including the production of flavorings. The aim of this research was to develop a flavoring agent from protein hydrolysates of free-range chicken bones that can be used in the food industry. The partial chemical composition of free-range chicken bones was studied, followed by enzymatic hydrolysis with the enzymes Alcalase, Flavourzyme and a mixture of the two enzymes (1:1). The protein hydrolysates were analyzed for free and total amino acid content, sugar profile and volatile profile. The free-range chicken bones had a high content of proteins and lipids, 26.27g/100g and 12.21g/100g, respectively; they were also rich in calcium (11,56g/100g) and phosphorus (4,55g/100g). The alcalase enzyme had a higher degree of hydrolysis (20.59%), compared to flavourzyme (4.60%) and the enzyme mixture (14.90%). The profile of free amino acids released by the enzymes favored the HFA - hydrolysate of Flavourzyme and the alcalase enzyme mixture, but the HF - hydrolysate of Flavourzyme provided volatile components in greater quantity and quality for meat flavor. The sugar profile indicated the presence of maltose, glucose, fructose, and ribose, with higher sugar concentrations in the dry residues of free-range chicken bones, suggesting that these sugars were consumed by the enzymatic hydrolysis. Glucose (FG) or xylose (FX) was added to the selected hydrolysate, HF, to obtain two flavorings that were tested for instrumental color, flavor profile, and sensory characterization. FX exhibited more browning after the Maillard reaction and eight more aroma components than FG. However, the sensory panel did not identify significant between the two flavorings. In summary, FX can be used as a basis for the development of meat flavors that will enable advances in environmental issues and cost improvements for free-range chicken production.
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spelling Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipiraReação de MaillardSubprodutos de frangoCompostos voláteisHidrolisado proteicoOssos de frangoMaillard reactionChicken by-productsVolatile compoundsProtein hydrolyzateChicken boneCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSOne of the most important by-products of chicken slaughter, bone residues, are an excellent source of protein and can therefore be used as protein hydrolysate obtained by enzymatic hydrolysis. This technique has allowed the use of meat by-products with a wide technological application, including the production of flavorings. The aim of this research was to develop a flavoring agent from protein hydrolysates of free-range chicken bones that can be used in the food industry. The partial chemical composition of free-range chicken bones was studied, followed by enzymatic hydrolysis with the enzymes Alcalase, Flavourzyme and a mixture of the two enzymes (1:1). The protein hydrolysates were analyzed for free and total amino acid content, sugar profile and volatile profile. The free-range chicken bones had a high content of proteins and lipids, 26.27g/100g and 12.21g/100g, respectively; they were also rich in calcium (11,56g/100g) and phosphorus (4,55g/100g). The alcalase enzyme had a higher degree of hydrolysis (20.59%), compared to flavourzyme (4.60%) and the enzyme mixture (14.90%). The profile of free amino acids released by the enzymes favored the HFA - hydrolysate of Flavourzyme and the alcalase enzyme mixture, but the HF - hydrolysate of Flavourzyme provided volatile components in greater quantity and quality for meat flavor. The sugar profile indicated the presence of maltose, glucose, fructose, and ribose, with higher sugar concentrations in the dry residues of free-range chicken bones, suggesting that these sugars were consumed by the enzymatic hydrolysis. Glucose (FG) or xylose (FX) was added to the selected hydrolysate, HF, to obtain two flavorings that were tested for instrumental color, flavor profile, and sensory characterization. FX exhibited more browning after the Maillard reaction and eight more aroma components than FG. However, the sensory panel did not identify significant between the two flavorings. In summary, FX can be used as a basis for the development of meat flavors that will enable advances in environmental issues and cost improvements for free-range chicken production.Conselho Nacional de Pesquisa e Desenvolvimento Científico e Tecnológico - CNPqUm dos principais subprodutos do abate do frango, o resíduo ósseo, constitui uma excelente fonte de proteínas e por esse motivo apresenta potencial para uso como hidrolisado proteico, obtido por hidrolise enzimática. Esta técnica tem possibilitado o aproveitamento de subprodutos cárneos, com ampla aplicação tecnológica, entre as quais cita-se a obtenção de flavorizantes. O objetivo desta pesquisa consistiu em elaborar flavorizante a partir de hidrolisados proteicos de ossos de frango caipira com potencial de utilização pela indústria de alimentos. Foram realizados estudos da composição química parcial dos ossos de frango caipira, seguido de hidrólise enzimática utilizando as enzimas Alcalase, Flavourzyme e uma mistura das duas enzimas (1:1). Os hidrolisados proteicos foram caracterizados em relação ao teor de aminoácidos livres e totais, perfil de açúcares e perfil volátil. Os ossos de frango caipira apresentaram altos percentuais de proteínas e lipídios, 26,27g/100g e 12,21g/100g respectivamente; além de serem ricos em cálcio (11,56g/100g) e fósforo (4,55g/100g). A enzima alcalase apresentou maior grau de hidrólise (20,59%), em comparação com a flavouryme (4,60%) e a mistura de enzimas (14,90%). O perfil de aminoácidos livres liberados pelas enzimas favoreceu a obtenção do hidrolisado proteico da mistura de enzimas flavourzyme e alcalase (HFA); no entanto, o hidrolisado obtido a partir da flavourzyme (HF) proporcionou componentes voláteis em maior quantidade e qualidade superior para o aroma cárneo. O perfil de açúcares sinalizou a presença de maltose, glicose, frutose e ribose, com maior concentração de açúcares no resíduo seco de ossos de frango caipira, indicando que houve o consumo desses açúcares durante a hidrólise enzimática. O hidrolisado selecionado, o HF, foi adicionado de glicose (FG) ou de xilose (FX) para obtenção de dois flavorizantes que foram avaliados nos parâmetros de L*, a*, b*, perfil aromático e qualidade sensorial. O flavorizante FX demonstrou maior escurecimento pós reação de Maillard e oito componentes de aroma a mais que o flavorizante FG; no entanto, o painel sensorial não identificou diferenças significativas entre os dois flavorizantes. Logo, conclui-se que o flavorizante adicionado de xilose (FX) apresenta potencial de utilização como base para o desenvolvimento de aromas cárneos, possibilitando avanços em questões ambientais e melhorias nos custos para o setor produtivo de frango caipira.Universidade Federal da ParaíbaBrasilEngenharia de AlimentosPrograma de Pós-Graduação em Ciência e Tecnologia de AlimentosUFPBBezerra, Taliana Kênia Alencarhttp://lattes.cnpq.br/3073326980952939Madruga, Marta Suelyhttp://lattes.cnpq.br/2316472151055499Cunha, Rebeka Correia de Souza2022-08-11T17:05:37Z2022-06-202022-08-11T17:05:37Z2022-03-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttps://repositorio.ufpb.br/jspui/handle/123456789/24206porAttribution-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2022-10-25T13:22:11Zoai:repositorio.ufpb.br:123456789/24206Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2022-10-25T13:22:11Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false
dc.title.none.fl_str_mv Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipira
title Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipira
spellingShingle Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipira
Cunha, Rebeka Correia de Souza
Reação de Maillard
Subprodutos de frango
Compostos voláteis
Hidrolisado proteico
Ossos de frango
Maillard reaction
Chicken by-products
Volatile compounds
Protein hydrolyzate
Chicken bone
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
title_short Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipira
title_full Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipira
title_fullStr Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipira
title_full_unstemmed Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipira
title_sort Obtenção de hidrolisados proteicos com potencial flavorizante a partir de osso de frango caipira
author Cunha, Rebeka Correia de Souza
author_facet Cunha, Rebeka Correia de Souza
author_role author
dc.contributor.none.fl_str_mv Bezerra, Taliana Kênia Alencar
http://lattes.cnpq.br/3073326980952939
Madruga, Marta Suely
http://lattes.cnpq.br/2316472151055499
dc.contributor.author.fl_str_mv Cunha, Rebeka Correia de Souza
dc.subject.por.fl_str_mv Reação de Maillard
Subprodutos de frango
Compostos voláteis
Hidrolisado proteico
Ossos de frango
Maillard reaction
Chicken by-products
Volatile compounds
Protein hydrolyzate
Chicken bone
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
topic Reação de Maillard
Subprodutos de frango
Compostos voláteis
Hidrolisado proteico
Ossos de frango
Maillard reaction
Chicken by-products
Volatile compounds
Protein hydrolyzate
Chicken bone
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS
description One of the most important by-products of chicken slaughter, bone residues, are an excellent source of protein and can therefore be used as protein hydrolysate obtained by enzymatic hydrolysis. This technique has allowed the use of meat by-products with a wide technological application, including the production of flavorings. The aim of this research was to develop a flavoring agent from protein hydrolysates of free-range chicken bones that can be used in the food industry. The partial chemical composition of free-range chicken bones was studied, followed by enzymatic hydrolysis with the enzymes Alcalase, Flavourzyme and a mixture of the two enzymes (1:1). The protein hydrolysates were analyzed for free and total amino acid content, sugar profile and volatile profile. The free-range chicken bones had a high content of proteins and lipids, 26.27g/100g and 12.21g/100g, respectively; they were also rich in calcium (11,56g/100g) and phosphorus (4,55g/100g). The alcalase enzyme had a higher degree of hydrolysis (20.59%), compared to flavourzyme (4.60%) and the enzyme mixture (14.90%). The profile of free amino acids released by the enzymes favored the HFA - hydrolysate of Flavourzyme and the alcalase enzyme mixture, but the HF - hydrolysate of Flavourzyme provided volatile components in greater quantity and quality for meat flavor. The sugar profile indicated the presence of maltose, glucose, fructose, and ribose, with higher sugar concentrations in the dry residues of free-range chicken bones, suggesting that these sugars were consumed by the enzymatic hydrolysis. Glucose (FG) or xylose (FX) was added to the selected hydrolysate, HF, to obtain two flavorings that were tested for instrumental color, flavor profile, and sensory characterization. FX exhibited more browning after the Maillard reaction and eight more aroma components than FG. However, the sensory panel did not identify significant between the two flavorings. In summary, FX can be used as a basis for the development of meat flavors that will enable advances in environmental issues and cost improvements for free-range chicken production.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-11T17:05:37Z
2022-06-20
2022-08-11T17:05:37Z
2022-03-03
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufpb.br/jspui/handle/123456789/24206
url https://repositorio.ufpb.br/jspui/handle/123456789/24206
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Engenharia de Alimentos
Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
UFPB
publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Engenharia de Alimentos
Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos
UFPB
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFPB
instname:Universidade Federal da Paraíba (UFPB)
instacron:UFPB
instname_str Universidade Federal da Paraíba (UFPB)
instacron_str UFPB
institution UFPB
reponame_str Biblioteca Digital de Teses e Dissertações da UFPB
collection Biblioteca Digital de Teses e Dissertações da UFPB
repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)
repository.mail.fl_str_mv diretoria@ufpb.br|| diretoria@ufpb.br
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