Atividade antifúngica in vitro do óleo essencial de Melissa officinalis L. (ERVA-CIDREIRA) SOBRE Cladosporium carrionii

• Autor(a) principal: Menezes, Camilla Pinheiro de
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/tede/6752
• topic: Óleo essencial; Lamiaceae; Melissa officinalis; Fungos dematiáceos; Cladosporium carrionii; Essential oil; Lamiaceae; Melissa officinalis; Dematiaceous fungi; Cladosporium carrionii; CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA

The genus includes many species of Cladosporium fungi and opportunistic dematiaceous contaminants being found in diverse environments. Cladosporium carrionii is considered the most important pathogenic species of this genus due to the numerous cases of disease caused by this fungus in the world, being an agent of chromoblastomycosis, feohifomicoses and allergies. The increased resistance to antifungal agents available and their toxicity lead to search for new antifungals, more effective and less toxic. In this context, this study aimed to identify the components of the essential oil of Melissa officinalis L. and investigate their antifungal activity in vitro against strains of Cladosporium carrionii. The chemical composition of the oil was performed by gas chromatography coupled to mass spectrometer (GC-MS). Assays of antifungal activity were performed by microbiological screening by diffusion technique in solid medium; determination of minimum inhibitory concentration (MIC) by microdilution; minimum fungicidal concentration (MFC); measure radial growth at different time intervals; Inhibition of conidial germination and assessment of morphological changes in the presence of the essential oil. The results of GC-MS showed 4 major components, being geranial (52%) the major constituent, followed by the citral (38.90%), trans-&#946;-caryophyllene (1.22%) and germacrene D (0.84%), in descending order of percentage. In antifungal activity assays, the essential oil of M. officinalis inhibited the growth of 100% of the strains of C. carrionii tested, and its MIC and MFC established at 256 mg / mL, and is considered a product with strong antifungal activity. Was able to induce radial mycelial growth inhibition from the concentration of 128 mg / ml (MIC/2), and the concentrations MICX2 and MICX4, there was complete inhibition of the growth. It was also observed significant inhibition of conidial germination in all concentrations compared to control (p <0.05), those potentiated inhibition with increased concentration so that the concentration was MICX2 100% inhibition of germination of conidia counted. Furthermore, morphological changes such as decreased conidiation and structural changes in hyphae were observed in the strains tested, after exposure to oil concentrations MIC/2, MIC and MICX2. Given the foregoing, it is concluded that the essential oil of M. officinalis has a significant antifungal activity against strains of C. carrionii, representing a new possibility in the arsenal of products for treatment of mycoses caused by Cladosporium carrionii.