Efeito de produtos lácteos caprinos sobre a doença inflamatória intestinal em modelo animal

• Autor(a) principal: Assis, Paloma Oliveira Antonino de
• Data de Publicação: 2015
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
• Texto Completo: https://repositorio.ufpb.br/jspui/handle/tede/8834
• topic: Leite de cabra; Probiótico; Iogurte; Mel; Alimento funcional; Colite; Goat milk; Probiotic; Yogurt; Honey; Functional food; Colitis; CIENCIAS DA SAUDE::NUTRICAO

Goat milk is a highly nutritious food that provides therapeutic and dietary characteristics, being an excellent source for deriving products that has functional potential, whose nutritional benefits can be improved by enriching them with probiotic strains. Milk and dairy products can provide nutritional support for patients with intestinal inflammation. Inflammatory Bowel Disease (IBD) is a debilitating and immunologically-mediated disease that leads to tissue destruction in the gastrointestinal tract, which comprises Crohn's disease (CD) and ulcerative colitis (UC). This study aimed at evaluating the intestinal anti-inflammatory effect of goat milk and goat yogurt with addition of Lactobacillus acidophilus, with or without native bee honey (Melipona scutellaris), in rats with induced colitis. In the experiment female wistar rats (190 a 240 g), were randomized in 7 groups (n = 10): Non-colitic; Colitic; Goat milk (GM); Goat yogurt (without honey) (GY); Goat yogurt with honey (10%) (GYH); Goat yogurt with honey (10%) twice a day (GYH/2x), and Sulfasalazine (250 mg/kg) (SAZ). The animals received 1 ml of the product via gavage during 14 days. After this period, colitis was induced with acetic acid (0.5 mL of 10% v/v in 0.9% saline). Forty eight hours after inducing the colitis, the animals were euthanized. Were assessed the macroscopic and microscopic damage of colitis, inflammatory parameters, expressed by myeloperoxidase (MPO), Tumor Necrosis Factor (TNF)-α, interleukin (IL)1-β, Cyclooxygenase (COX)-2 and Inducible Nitric Oxide Synthase (iNOS) and oxidative stress markers, such as malondialdehyde (MDA), and total glutathione. The pre-treatment with goat milk, goat yogurt or sulfasalazine resulted in a significant reduction of the macroscopic damage score (p <0.01 vs. colitic group), and improved the MPO activity (p <0.01 vs. colitic group), the levels of pro-inflammatory citokynes, TNF-α (p <0.01 vs. colitic group) (p <0.01 SAZ vs. LC, ICM e ICM/2x) and IL1-β (p <0.01 vs. colitic group). It also promoted a significant reduction in oxidative stress that could be observed by the reduction in the MDA (p <0,01 vs. colitic group) (p <0.05 LC vs. SAZ) and the increase in the glutathione (p <0.01 vs. colitic group) (p <0.01 SAZ vs. LC, IC e ICM/2x). The benefits of the pre-treatments were also demonstrated by the preservation of the colonic cytoarchitecture, and the decreased expression of the COX-2 (p <0.05 vs. colitic group) and the iNOS (p <0.05 vs. colitic group). In conclusion, it was observed that goat milk, goat yogurt and sulfasalazine exerted anti-inflammatory effect on colitis model induced by acetic acid in rats. Therefore, these goat dairy products may be a valuable alternatives to traditional medications and a potential functional food for the prevention of IBD.