Novas Saponinas Isoladas de Cereus jamacaru

Detalhes bibliográficos
Autor(a) principal: Brito, Analu Freitas de Souza
Data de Publicação: 2018
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UFPB
Texto Completo: https://repositorio.ufpb.br/jspui/handle/123456789/15137
Resumo: Cereus jamacaru belongs to the family Cactaceae, is popularly known in Brazil by mandacaru, occurring in the states of Piauí, Paraíba, Ceará, Sergipe, Rio Grande do Norte, Alagoas, Pernambuco, Bahia and northern Minas Gerais. The popular use of this species is reported in the treatment of urethral problems, syphilis, pain in the cervical spine, being indicated also in the control of diabetes, in the treatment of vesicular stones, problems in the respiratory apparatus such as coughs and bronchitis, as well as ulcers. This work aimed to contribute to the expansion of the chemical knowledge of the genus Cereus through the phytochemical study of Cereus jamacaru. For this, the vegetable material, after drying and ground, was subjected to a maceration with 95% ethanol for 72 hours, this process being repeated three times to obtain the crude ethanolic extract (BSE). An aliquot of the BSE (30.0g) was subjected to a liquid-liquid partition in a separatory funnel with the solvents hexane, dichloromethane and ethyl acetate. The ethyl acetate fraction was subjected to column chromatography using Sephadex to perform a previous fractionation of this fraction. Then an analytical method was developed by HPLC-DAD for the analysis of fractions 5-10, later transposed to a semipreparative scale, thus it is possible to isolate a saponin (CJ1) reported for the first time in the literature. Afterwards, a method of isolation by HPLC-ESI-EM / EM was developed, thus it is possible to isolate another saponin (CJ2), also reported for the first time in the literature. In this way, this work revealed this species as a bioproducer of triterpene saponins.
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spelling Novas Saponinas Isoladas de Cereus jamacaruCereus jamacaruSaponinasCromatografiaEspectrometria de massasSaponinsChromatographyMass spectrometryCactos - Cereus JamacaruCactáceas - Substâncias isoladasPlantas medicinais - EficáciaCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIACereus jamacaru belongs to the family Cactaceae, is popularly known in Brazil by mandacaru, occurring in the states of Piauí, Paraíba, Ceará, Sergipe, Rio Grande do Norte, Alagoas, Pernambuco, Bahia and northern Minas Gerais. The popular use of this species is reported in the treatment of urethral problems, syphilis, pain in the cervical spine, being indicated also in the control of diabetes, in the treatment of vesicular stones, problems in the respiratory apparatus such as coughs and bronchitis, as well as ulcers. This work aimed to contribute to the expansion of the chemical knowledge of the genus Cereus through the phytochemical study of Cereus jamacaru. For this, the vegetable material, after drying and ground, was subjected to a maceration with 95% ethanol for 72 hours, this process being repeated three times to obtain the crude ethanolic extract (BSE). An aliquot of the BSE (30.0g) was subjected to a liquid-liquid partition in a separatory funnel with the solvents hexane, dichloromethane and ethyl acetate. The ethyl acetate fraction was subjected to column chromatography using Sephadex to perform a previous fractionation of this fraction. Then an analytical method was developed by HPLC-DAD for the analysis of fractions 5-10, later transposed to a semipreparative scale, thus it is possible to isolate a saponin (CJ1) reported for the first time in the literature. Afterwards, a method of isolation by HPLC-ESI-EM / EM was developed, thus it is possible to isolate another saponin (CJ2), also reported for the first time in the literature. In this way, this work revealed this species as a bioproducer of triterpene saponins.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESCereus jamacaru pertence à família Cactaceae, é popularmente conhecido no Brasil por mandacaru, com ocorrência nos estados do Piauí, Paraíba, Ceará, Sergipe, Rio Grande do Norte, Alagoas, Pernambuco, Bahia e norte de Minas Gerais. O uso popular desta espécie é relatado no tratamento de problemas uretrais, sífilis, dores na coluna cervical, sendo indicado também no controle de diabetes, no tratamento de pedras vesiculares, problemas no aparelho respiratório como tosses e bronquites, além de úlceras. Este trabalho teve como objetivo contribuir com a ampliação do conhecimento químico do gênero Cereus através do estudo fitoquímico de Cereus jamacaru. Para isto, o material vegetal, após seco e moído, foi submetido a uma maceração com etanol a 95% por 72 horas, sendo esse processo repetido por três vezes, obtendo-se o extrato etanólico bruto (EEB). Uma alíquota do EEB (30,0g) foi submetida a uma partição líquido-líquido em uma ampola de separação com os solventes hexano, diclorometano e acetato de etila. A fração acetato de etila foi submetida a uma cromatografia em coluna utilizando Sephadex para realizar um fracionamento prévio desta fração. Em seguida foi desenvolvido um método analítico por CLAE-DAD para a análise das frações 5-10, posteriormente, transposto para uma escala semipreparativa, sendo assim possível isolar uma saponina (CJ1) relatada pela primeira vez na literatura. Posteriormente, foi desenvolvido um método de isolamento por CLAE-ESI-EM/EM, sendo assim possível isolar uma outra saponina (CJ2), também relatada pela primeira vez na literatura. Desta forma, este trabalho revelou esta espécie como bioprodutora de saponinas triterpênicas.Universidade Federal da ParaíbaBrasilFarmacologiaPrograma de Pós-Graduação em Produtos Naturais e Sintéticos BioativosUFPBTavares , Josean Fechinehttp://lattes.cnpq.br/6009412640611523Brito, Analu Freitas de Souza2019-07-23T14:27:49Z2019-02-102019-07-23T14:27:49Z2018-12-13info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttps://repositorio.ufpb.br/jspui/handle/123456789/15137porAttribution-NoDerivs 3.0 Brazilhttp://creativecommons.org/licenses/by-nd/3.0/br/info:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UFPBinstname:Universidade Federal da Paraíba (UFPB)instacron:UFPB2019-07-24T06:01:16Zoai:repositorio.ufpb.br:123456789/15137Biblioteca Digital de Teses e Dissertaçõeshttps://repositorio.ufpb.br/PUBhttp://tede.biblioteca.ufpb.br:8080/oai/requestdiretoria@ufpb.br|| diretoria@ufpb.bropendoar:2019-07-24T06:01:16Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)false
dc.title.none.fl_str_mv Novas Saponinas Isoladas de Cereus jamacaru
title Novas Saponinas Isoladas de Cereus jamacaru
spellingShingle Novas Saponinas Isoladas de Cereus jamacaru
Brito, Analu Freitas de Souza
Cereus jamacaru
Saponinas
Cromatografia
Espectrometria de massas
Saponins
Chromatography
Mass spectrometry
Cactos - Cereus Jamacaru
Cactáceas - Substâncias isoladas
Plantas medicinais - Eficácia
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
title_short Novas Saponinas Isoladas de Cereus jamacaru
title_full Novas Saponinas Isoladas de Cereus jamacaru
title_fullStr Novas Saponinas Isoladas de Cereus jamacaru
title_full_unstemmed Novas Saponinas Isoladas de Cereus jamacaru
title_sort Novas Saponinas Isoladas de Cereus jamacaru
author Brito, Analu Freitas de Souza
author_facet Brito, Analu Freitas de Souza
author_role author
dc.contributor.none.fl_str_mv Tavares , Josean Fechine
http://lattes.cnpq.br/6009412640611523
dc.contributor.author.fl_str_mv Brito, Analu Freitas de Souza
dc.subject.por.fl_str_mv Cereus jamacaru
Saponinas
Cromatografia
Espectrometria de massas
Saponins
Chromatography
Mass spectrometry
Cactos - Cereus Jamacaru
Cactáceas - Substâncias isoladas
Plantas medicinais - Eficácia
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
topic Cereus jamacaru
Saponinas
Cromatografia
Espectrometria de massas
Saponins
Chromatography
Mass spectrometry
Cactos - Cereus Jamacaru
Cactáceas - Substâncias isoladas
Plantas medicinais - Eficácia
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA
description Cereus jamacaru belongs to the family Cactaceae, is popularly known in Brazil by mandacaru, occurring in the states of Piauí, Paraíba, Ceará, Sergipe, Rio Grande do Norte, Alagoas, Pernambuco, Bahia and northern Minas Gerais. The popular use of this species is reported in the treatment of urethral problems, syphilis, pain in the cervical spine, being indicated also in the control of diabetes, in the treatment of vesicular stones, problems in the respiratory apparatus such as coughs and bronchitis, as well as ulcers. This work aimed to contribute to the expansion of the chemical knowledge of the genus Cereus through the phytochemical study of Cereus jamacaru. For this, the vegetable material, after drying and ground, was subjected to a maceration with 95% ethanol for 72 hours, this process being repeated three times to obtain the crude ethanolic extract (BSE). An aliquot of the BSE (30.0g) was subjected to a liquid-liquid partition in a separatory funnel with the solvents hexane, dichloromethane and ethyl acetate. The ethyl acetate fraction was subjected to column chromatography using Sephadex to perform a previous fractionation of this fraction. Then an analytical method was developed by HPLC-DAD for the analysis of fractions 5-10, later transposed to a semipreparative scale, thus it is possible to isolate a saponin (CJ1) reported for the first time in the literature. Afterwards, a method of isolation by HPLC-ESI-EM / EM was developed, thus it is possible to isolate another saponin (CJ2), also reported for the first time in the literature. In this way, this work revealed this species as a bioproducer of triterpene saponins.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-13
2019-07-23T14:27:49Z
2019-02-10
2019-07-23T14:27:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://repositorio.ufpb.br/jspui/handle/123456789/15137
url https://repositorio.ufpb.br/jspui/handle/123456789/15137
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv Attribution-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nd/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution-NoDerivs 3.0 Brazil
http://creativecommons.org/licenses/by-nd/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Farmacologia
Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos
UFPB
publisher.none.fl_str_mv Universidade Federal da Paraíba
Brasil
Farmacologia
Programa de Pós-Graduação em Produtos Naturais e Sintéticos Bioativos
UFPB
dc.source.none.fl_str_mv reponame:Biblioteca Digital de Teses e Dissertações da UFPB
instname:Universidade Federal da Paraíba (UFPB)
instacron:UFPB
instname_str Universidade Federal da Paraíba (UFPB)
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repository.name.fl_str_mv Biblioteca Digital de Teses e Dissertações da UFPB - Universidade Federal da Paraíba (UFPB)
repository.mail.fl_str_mv diretoria@ufpb.br|| diretoria@ufpb.br
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