Atividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos orais
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2231 |
Resumo: | A great variety of diseases can affect the oral cavity, such as dental caries, that is considered the most prevalent oral disease worldwide. The multifactorial and infectcontagious characteristics of dental caries have been established, associated with resident bacterial pathogens of dental biofilm. These microorganisms are responsible by the production of acids and citotoxic products, which could respectively promote the demineralization of dental structure and gingival inflammation, with posterior destruction of supporting tissues. Therefore, the use of efficient antimicrobial agents against pathogenic bacteria is an important tool in the control of oral diseases. Several natural antimicrobial agents are been investigated because of their possible pharmacological properties. The mushroom Agaricus brasiliensis S. Wasser et al. ( Royal-Sun-Agaricus ) is pointed out as a natural product with noticeable antibacterial, antifungicidal, anti-inflammatory and antineoplasic properties. In this study the potential effect of this natural product was evaluated using three ethanolic crude extracts (100%, 75% and 50% EtOH) on growth and adherence of mutans streptococci. The ethanolic extracts were obtained by maceration process. Negative controls were 100%, 75% and 50% ethanol. The antimicrobial tests used against Streptococcus mutans UA 159 and Streptococcus sobrinus 6715 were agar diffusion method, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and bacterial cell adherence to a glass surface. In agar diffusion method, only the 100% etanol extract revealed a slight inhibiton of bacterial growth. Yet, in MIC and MBC tests, the three extracts presented antimicrobial properties and MIC and MBC values ranged between 87.4 and 444.5 μg/mL. The MBC for the three ethanolic extracts was similar to MIC against S. mutans, but only 75% ethanol extract was bactericidal against S. sobrinus. Among the ethanolic extracts tested, 100% ethanol extract was the most active (MIC 87.4μg/mL). The celular adherence also was inhibited at concentrations from 100 to 400 μg/mL of 50% and 75% ethanol extracts. In conclusion, the ethanolic extracts from A. brasiliensis showed remarkable inhibitory effects on bacterial growth and the 100% ethanol extract was the most active |
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http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776008U0http://lattes.cnpq.br/1812396380224339Ribeiro, Gladis Averhttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780566Y3&dataRevisao=nullDel Pino, Francisco Augusto BurkertLund, Rafael Guerra2014-08-20T14:30:07Z2013-01-162014-08-20T14:30:07Z2007-02-05LUND, Rafael Guerra. Antimicrobial activity of different ethanolic extracts of Agaricus brasiliensis S. Wasser et al. against oral streptococci. 2007. 71 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2007.http://repositorio.ufpel.edu.br/handle/ri/2231A great variety of diseases can affect the oral cavity, such as dental caries, that is considered the most prevalent oral disease worldwide. The multifactorial and infectcontagious characteristics of dental caries have been established, associated with resident bacterial pathogens of dental biofilm. These microorganisms are responsible by the production of acids and citotoxic products, which could respectively promote the demineralization of dental structure and gingival inflammation, with posterior destruction of supporting tissues. Therefore, the use of efficient antimicrobial agents against pathogenic bacteria is an important tool in the control of oral diseases. Several natural antimicrobial agents are been investigated because of their possible pharmacological properties. The mushroom Agaricus brasiliensis S. Wasser et al. ( Royal-Sun-Agaricus ) is pointed out as a natural product with noticeable antibacterial, antifungicidal, anti-inflammatory and antineoplasic properties. In this study the potential effect of this natural product was evaluated using three ethanolic crude extracts (100%, 75% and 50% EtOH) on growth and adherence of mutans streptococci. The ethanolic extracts were obtained by maceration process. Negative controls were 100%, 75% and 50% ethanol. The antimicrobial tests used against Streptococcus mutans UA 159 and Streptococcus sobrinus 6715 were agar diffusion method, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and bacterial cell adherence to a glass surface. In agar diffusion method, only the 100% etanol extract revealed a slight inhibiton of bacterial growth. Yet, in MIC and MBC tests, the three extracts presented antimicrobial properties and MIC and MBC values ranged between 87.4 and 444.5 μg/mL. The MBC for the three ethanolic extracts was similar to MIC against S. mutans, but only 75% ethanol extract was bactericidal against S. sobrinus. Among the ethanolic extracts tested, 100% ethanol extract was the most active (MIC 87.4μg/mL). The celular adherence also was inhibited at concentrations from 100 to 400 μg/mL of 50% and 75% ethanol extracts. In conclusion, the ethanolic extracts from A. brasiliensis showed remarkable inhibitory effects on bacterial growth and the 100% ethanol extract was the most activeUma variedade de doenças envolve a cavidade oral, como a cárie dental, considerada a patologia oral mais prevalente em todo o mundo. Atualmente, a cárie dental é uma doença multifatorial infecto-contagiosa, associada a bactérias patogênicas residentes do biofilme dental. Estes microrganismos são os responsáveis pela produção de ácidos e produtos citotóxicos que, respectivamente, levam à desmineralização de esmalte dental e inflamação gengival com posterior destruição dos tecidos de suporte dos dentes. Assim, o uso de agentes antimicrobianos eficientes contra essas bactérias é um importante meio de controle dessa doença bucal. Vários agentes antimicrobianos de origem natural estão sendo investigados devido as suas possíveis propriedades farmacológicas. O cogumelo Agaricus brasiliensis S. Wasser et al. ( cogumelo-do-sol) destaca-se entre os produtos naturais com notáveis propriedades antibacterianas, antifúngicas, antiinflamatórias e antitumorais. Neste estudo foi avaliado o efeito de três extratos etanólicos brutos (EtOH 100%, 75% e 50%) deste cogumelo sobre o crescimento e a aderência de estreptococos do grupo mutans. Os extratos etanólicos foram obtidos através do método de extração por maceração. Os controles negativos foram etanol 100%, 75% e 50%. Os testes antimicrobianos utilizados contra Streptococcus mutans UA 159 e Streptococcus sobrinus 6715 foram o método de difusão em ágar, Concentração Inibitória Mínima (CIM), Concentração Bactericida Mínima (CBM) e aderência dos microrganismos à superfície de vidro (Adh). No método de difusão em agar, apenas o extrato 100% etanólico apresentou sinal de inibição do crescimento microbiano. Já nos testes de determinação da CIM e CBM, os três extratos apresentaram propriedades antimicrobianas e os valores de CIM e CBM variaram entre 87,4 e 444,5 μg/mL. A CBM para os três extratos etanólicos foi igual a CIM para S.sobrinus, mas apenas o extrato etanólico 75% foi bactericida para S. mutans. Entre os extratos etanólicos testados, o extrato 100% etanólico foi o mais ativo (CIM 87.4μg/mL). A aderência celular também foi inibida em concentrações de 100 a 400μg/mL dos extratos obtidos com etanol 50% e 75%. Concluindo, os extratos etanólicos de A. brasiliensis mostraram notáveis efeitos inibitórios no crescimento bacteriano e o extrato 100% etanólico foi o mais ativoapplication/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em OdontologiaUFPelBROdontologiaDentísticaAgentes cariostáticosCogumelos medicinaisStreptococcus mutansCogumelo-do-solAgentesAntimicrobianosBiofilmesDentistryCariostatic agentsMedicinal mushroomsBiofilmsStreptococcus mutansRoyal-Sun-AgaricusAntimicrobial agentsCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAAtividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos oraisAntimicrobial activity of different ethanolic extracts of Agaricus brasiliensis S. Wasser et al. against oral streptococciinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_rafael_guerra_lund.pdfapplication/pdf2856587http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2231/1/dissertacao_rafael_guerra_lund.pdf01149241eaab789cb42b16915e1cbebdMD51open accessTEXTdissertacao_rafael_guerra_lund.pdf.txtdissertacao_rafael_guerra_lund.pdf.txtExtracted Texttext/plain89495http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2231/2/dissertacao_rafael_guerra_lund.pdf.txt48eb636c84eefc3a9cd93600a24ec649MD52open accessTHUMBNAILdissertacao_rafael_guerra_lund.pdf.jpgdissertacao_rafael_guerra_lund.pdf.jpgGenerated Thumbnailimage/jpeg1386http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2231/3/dissertacao_rafael_guerra_lund.pdf.jpgedb5a008edca3a3668671c41483187dcMD53open access123456789/22312019-08-16 21:04:13.803open accessoai:guaiaca.ufpel.edu.br:123456789/2231Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-17T00:04:13Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Atividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos orais |
dc.title.alternative.eng.fl_str_mv |
Antimicrobial activity of different ethanolic extracts of Agaricus brasiliensis S. Wasser et al. against oral streptococci |
title |
Atividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos orais |
spellingShingle |
Atividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos orais Lund, Rafael Guerra Dentística Agentes cariostáticos Cogumelos medicinais Streptococcus mutans Cogumelo-do-sol Agentes Antimicrobianos Biofilmes Dentistry Cariostatic agents Medicinal mushrooms Biofilms Streptococcus mutans Royal-Sun-Agaricus Antimicrobial agents CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Atividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos orais |
title_full |
Atividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos orais |
title_fullStr |
Atividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos orais |
title_full_unstemmed |
Atividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos orais |
title_sort |
Atividade antimicrobiana de diferentes extratos etanólicos de Agaricus brasiliensis S. Wasser et al. em estreptococos orais |
author |
Lund, Rafael Guerra |
author_facet |
Lund, Rafael Guerra |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776008U0 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/1812396380224339 |
dc.contributor.advisor-co1.fl_str_mv |
Ribeiro, Gladis Aver |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780566Y3&dataRevisao=null |
dc.contributor.advisor1.fl_str_mv |
Del Pino, Francisco Augusto Burkert |
dc.contributor.author.fl_str_mv |
Lund, Rafael Guerra |
contributor_str_mv |
Ribeiro, Gladis Aver Del Pino, Francisco Augusto Burkert |
dc.subject.por.fl_str_mv |
Dentística Agentes cariostáticos Cogumelos medicinais Streptococcus mutans Cogumelo-do-sol Agentes Antimicrobianos Biofilmes |
topic |
Dentística Agentes cariostáticos Cogumelos medicinais Streptococcus mutans Cogumelo-do-sol Agentes Antimicrobianos Biofilmes Dentistry Cariostatic agents Medicinal mushrooms Biofilms Streptococcus mutans Royal-Sun-Agaricus Antimicrobial agents CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Dentistry Cariostatic agents Medicinal mushrooms Biofilms Streptococcus mutans Royal-Sun-Agaricus Antimicrobial agents |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
A great variety of diseases can affect the oral cavity, such as dental caries, that is considered the most prevalent oral disease worldwide. The multifactorial and infectcontagious characteristics of dental caries have been established, associated with resident bacterial pathogens of dental biofilm. These microorganisms are responsible by the production of acids and citotoxic products, which could respectively promote the demineralization of dental structure and gingival inflammation, with posterior destruction of supporting tissues. Therefore, the use of efficient antimicrobial agents against pathogenic bacteria is an important tool in the control of oral diseases. Several natural antimicrobial agents are been investigated because of their possible pharmacological properties. The mushroom Agaricus brasiliensis S. Wasser et al. ( Royal-Sun-Agaricus ) is pointed out as a natural product with noticeable antibacterial, antifungicidal, anti-inflammatory and antineoplasic properties. In this study the potential effect of this natural product was evaluated using three ethanolic crude extracts (100%, 75% and 50% EtOH) on growth and adherence of mutans streptococci. The ethanolic extracts were obtained by maceration process. Negative controls were 100%, 75% and 50% ethanol. The antimicrobial tests used against Streptococcus mutans UA 159 and Streptococcus sobrinus 6715 were agar diffusion method, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC) and bacterial cell adherence to a glass surface. In agar diffusion method, only the 100% etanol extract revealed a slight inhibiton of bacterial growth. Yet, in MIC and MBC tests, the three extracts presented antimicrobial properties and MIC and MBC values ranged between 87.4 and 444.5 μg/mL. The MBC for the three ethanolic extracts was similar to MIC against S. mutans, but only 75% ethanol extract was bactericidal against S. sobrinus. Among the ethanolic extracts tested, 100% ethanol extract was the most active (MIC 87.4μg/mL). The celular adherence also was inhibited at concentrations from 100 to 400 μg/mL of 50% and 75% ethanol extracts. In conclusion, the ethanolic extracts from A. brasiliensis showed remarkable inhibitory effects on bacterial growth and the 100% ethanol extract was the most active |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-02-05 |
dc.date.available.fl_str_mv |
2013-01-16 2014-08-20T14:30:07Z |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:30:07Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
LUND, Rafael Guerra. Antimicrobial activity of different ethanolic extracts of Agaricus brasiliensis S. Wasser et al. against oral streptococci. 2007. 71 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2007. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2231 |
identifier_str_mv |
LUND, Rafael Guerra. Antimicrobial activity of different ethanolic extracts of Agaricus brasiliensis S. Wasser et al. against oral streptococci. 2007. 71 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2007. |
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http://repositorio.ufpel.edu.br/handle/ri/2231 |
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Universidade Federal de Pelotas |
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UFPel |
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Odontologia |
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Universidade Federal de Pelotas |
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