Transferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusariose
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://guaiaca.ufpel.edu.br/handle/123456789/1255 |
Resumo: | Fusarium head blight (FHB) is one of the most important diseases known to affect wheat crop. FHB, caused mainly by Fusarium graminearum, results in severe losses of productivity and grain quality besides its harmful effects to human and animal health due to the accumulation of mycotoxins in infected grains. Genetic resistance is the best way to control FHB which affects crops in several parts of the world. FHB resistance shows a quantitative heritage, hence are QTLs (quantitativetrait- loci) that have a weak effect on character. Studies with microsatellite markers have generated a great number of information related to the position of those QTLs in the genome, in addition to the development of new varieties to be used in breeding programs. The microsatellites are short sequence repeats, spread thoroughly in the organism s genome. For amplification of those sequences it is used specific primers to each loco. Due to the genetic relation between species, information generated from the study in one species can be used successfully when studying related species. Therefore, primers designed for microsatellites in one species can be used to detect markers in related species and to associating those markers with characteristics of interest. Here, the main objective of my work was to verify the transferability of microsatellites from rice to wheat in search of markers related to FHB resistance. It was tested different wheat genotypes showing different levels of resistance to Fusarium and was verified the existence of polymorphism among those genotypes. A number of 55 primer pairs of microsatellites isolated from the rice genome were tested in 13 wheat cultivars classified as susceptible, moderately susceptible, moderately resistant and resistant. In my results, the rate of microsatellite transferability was of 76.4%, where from these, 23.8% showed polymorphism and 76.2% showed monomorphism. In spite of the high rate of the microsatellite transferability observed in my results, the analysis of polymorphism did not allow the identification of markers that could be possibly associated with resistance to Fusarium head blight in wheat. |
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2014-08-20T13:32:52Z2008-04-292014-08-20T13:32:52Z2007-12-29CARVALHO, Alexandre Zanardo de. Transferability of microsatellite from rice to wheat in search of markers related to Fusarium head blight resistance. 2007. 54 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2007.http://guaiaca.ufpel.edu.br/handle/123456789/1255Fusarium head blight (FHB) is one of the most important diseases known to affect wheat crop. FHB, caused mainly by Fusarium graminearum, results in severe losses of productivity and grain quality besides its harmful effects to human and animal health due to the accumulation of mycotoxins in infected grains. Genetic resistance is the best way to control FHB which affects crops in several parts of the world. FHB resistance shows a quantitative heritage, hence are QTLs (quantitativetrait- loci) that have a weak effect on character. Studies with microsatellite markers have generated a great number of information related to the position of those QTLs in the genome, in addition to the development of new varieties to be used in breeding programs. The microsatellites are short sequence repeats, spread thoroughly in the organism s genome. For amplification of those sequences it is used specific primers to each loco. Due to the genetic relation between species, information generated from the study in one species can be used successfully when studying related species. Therefore, primers designed for microsatellites in one species can be used to detect markers in related species and to associating those markers with characteristics of interest. Here, the main objective of my work was to verify the transferability of microsatellites from rice to wheat in search of markers related to FHB resistance. It was tested different wheat genotypes showing different levels of resistance to Fusarium and was verified the existence of polymorphism among those genotypes. A number of 55 primer pairs of microsatellites isolated from the rice genome were tested in 13 wheat cultivars classified as susceptible, moderately susceptible, moderately resistant and resistant. In my results, the rate of microsatellite transferability was of 76.4%, where from these, 23.8% showed polymorphism and 76.2% showed monomorphism. In spite of the high rate of the microsatellite transferability observed in my results, the analysis of polymorphism did not allow the identification of markers that could be possibly associated with resistance to Fusarium head blight in wheat.A fusariose do trigo, causada principalmente pelo fungo Fusarium graminearum, é uma das principais doenças nessa cultura, ocasionando perda de produtividade e de qualidade de grãos, além dos efeitos nocivos à saúde do homem e de animais devido ao acúmulo de micotoxinas nos grãos infectados. A resistência genética é a melhor forma para o controle dessa patologia que atinge a cultura em várias regiões do mundo. Ela exibe herança quantitativa, ou seja, são QTLs que tem um pequeno efeito sobre o caráter. Estudos com marcadores de microssatélites têm gerado um grande número de informações a respeito da posição desses QTLs no genoma e no desenvolvimento de novas variedades para cultivo em programas de melhoramento. Os microssatélites são pequenas seqüências de bases repetidas, posicionadas adjacentemente e distribuídas amplamente no genoma dos organismos. Para sua amplificação são utilizados primers específicos para cada loco. As relações genéticas entre espécies permitem que informações geradas a partir do estudo de uma delas sejam utilizadas para o estudo de espécies relacionadas. Dessa forma, primers desenhados para locos de microssatélites em uma espécie podem ser utilizados para detectar marcadores em espécies afins e serem associados a características de interesse. Sendo assim, o objetivo deste trabalho foi verificar a transferabilidade de locos de microssatélites de arroz para genótipos de trigo com diferentes níveis de resistência à fusariose e verificar a existência de polimorfismo entre os genótipos contrastantes que pudessem ser associados à característica de resistência. Foram testados 55 pares de primers de locos de microssatélites, isolados do genoma do arroz, em 13 cultivares de trigo, classificadas como suscetíveis, moderadamente suscetíveis, moderadamente resistentes e resistentes. Houve a transferabilidade de 76,4% desses locos de microssatélites. Destes, 23,8% apresentaram polimorfismo e o restante, 76,2%, apresentaram-se monomórficos. A análise do polimorfismo não permitiu a identificação de marcadores que pudessem ser associados à resistência para fusariose do trigo.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em BiotecnologiaUFPelBRBiotecnologiaMolecular markersGenetic improvementMarcadores molecularesMelhoramento genéticoSSRCNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::PRODUCAO E BENEFICIAMENTO DE SEMENTESTransferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusarioseTransferability of microsatellite from rice to wheat in search of markers related to Fusarium head blight resistanceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/9233403844402037http://lattes.cnpq.br/0540625238777673Peters, José AntônioCarvalho, Alexandre Zanardo deinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_alexandre_zanardo_carvalho.pdfapplication/pdf599901http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1255/1/dissertacao_alexandre_zanardo_carvalho.pdf46bcadb2c2ae2efc2be1d2eb92a5f846MD51open accessTEXTdissertacao_alexandre_zanardo_carvalho.pdf.txtdissertacao_alexandre_zanardo_carvalho.pdf.txtExtracted Texttext/plain102354http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1255/2/dissertacao_alexandre_zanardo_carvalho.pdf.txtbd548dc20b97ce5c0be8f64e38cf8985MD52open accessTHUMBNAILdissertacao_alexandre_zanardo_carvalho.pdf.jpgdissertacao_alexandre_zanardo_carvalho.pdf.jpgGenerated Thumbnailimage/jpeg1799http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1255/3/dissertacao_alexandre_zanardo_carvalho.pdf.jpg0ada016c73fc3d088d3ed1a020bd8acdMD53open access123456789/12552019-08-23 10:47:36.685open accessoai:guaiaca.ufpel.edu.br:123456789/1255Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-23T13:47:36Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Transferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusariose |
dc.title.alternative.eng.fl_str_mv |
Transferability of microsatellite from rice to wheat in search of markers related to Fusarium head blight resistance |
title |
Transferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusariose |
spellingShingle |
Transferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusariose Carvalho, Alexandre Zanardo de Molecular markers Genetic improvement Marcadores moleculares Melhoramento genético SSR CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::PRODUCAO E BENEFICIAMENTO DE SEMENTES |
title_short |
Transferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusariose |
title_full |
Transferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusariose |
title_fullStr |
Transferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusariose |
title_full_unstemmed |
Transferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusariose |
title_sort |
Transferabilidade de microssatélites de arroz para trigo na busca por marcadores ligados à resistência à fusariose |
author |
Carvalho, Alexandre Zanardo de |
author_facet |
Carvalho, Alexandre Zanardo de |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/9233403844402037 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/0540625238777673 |
dc.contributor.advisor1.fl_str_mv |
Peters, José Antônio |
dc.contributor.author.fl_str_mv |
Carvalho, Alexandre Zanardo de |
contributor_str_mv |
Peters, José Antônio |
dc.subject.eng.fl_str_mv |
Molecular markers Genetic improvement |
topic |
Molecular markers Genetic improvement Marcadores moleculares Melhoramento genético SSR CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::PRODUCAO E BENEFICIAMENTO DE SEMENTES |
dc.subject.por.fl_str_mv |
Marcadores moleculares Melhoramento genético SSR |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::AGRONOMIA::FITOTECNIA::PRODUCAO E BENEFICIAMENTO DE SEMENTES |
description |
Fusarium head blight (FHB) is one of the most important diseases known to affect wheat crop. FHB, caused mainly by Fusarium graminearum, results in severe losses of productivity and grain quality besides its harmful effects to human and animal health due to the accumulation of mycotoxins in infected grains. Genetic resistance is the best way to control FHB which affects crops in several parts of the world. FHB resistance shows a quantitative heritage, hence are QTLs (quantitativetrait- loci) that have a weak effect on character. Studies with microsatellite markers have generated a great number of information related to the position of those QTLs in the genome, in addition to the development of new varieties to be used in breeding programs. The microsatellites are short sequence repeats, spread thoroughly in the organism s genome. For amplification of those sequences it is used specific primers to each loco. Due to the genetic relation between species, information generated from the study in one species can be used successfully when studying related species. Therefore, primers designed for microsatellites in one species can be used to detect markers in related species and to associating those markers with characteristics of interest. Here, the main objective of my work was to verify the transferability of microsatellites from rice to wheat in search of markers related to FHB resistance. It was tested different wheat genotypes showing different levels of resistance to Fusarium and was verified the existence of polymorphism among those genotypes. A number of 55 primer pairs of microsatellites isolated from the rice genome were tested in 13 wheat cultivars classified as susceptible, moderately susceptible, moderately resistant and resistant. In my results, the rate of microsatellite transferability was of 76.4%, where from these, 23.8% showed polymorphism and 76.2% showed monomorphism. In spite of the high rate of the microsatellite transferability observed in my results, the analysis of polymorphism did not allow the identification of markers that could be possibly associated with resistance to Fusarium head blight in wheat. |
publishDate |
2007 |
dc.date.issued.fl_str_mv |
2007-12-29 |
dc.date.available.fl_str_mv |
2008-04-29 2014-08-20T13:32:52Z |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:32:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARVALHO, Alexandre Zanardo de. Transferability of microsatellite from rice to wheat in search of markers related to Fusarium head blight resistance. 2007. 54 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2007. |
dc.identifier.uri.fl_str_mv |
http://guaiaca.ufpel.edu.br/handle/123456789/1255 |
identifier_str_mv |
CARVALHO, Alexandre Zanardo de. Transferability of microsatellite from rice to wheat in search of markers related to Fusarium head blight resistance. 2007. 54 f. Dissertação (Mestrado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2007. |
url |
http://guaiaca.ufpel.edu.br/handle/123456789/1255 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Biotecnologia |
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UFPel |
dc.publisher.country.fl_str_mv |
BR |
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Biotecnologia |
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Universidade Federal de Pelotas |
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