Ocorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do Sul
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/1319 |
Resumo: | Listeria monocytogenes is a Gram-positive of ubiquitous natural, psychrotropic pathogen which colonizes the surfaces of equipment and utensils in food processing industries. Milk and dairy products, especially cheeses with medium and high humidity have been implicated in listeriosis cases and outbreaks. Microbial contamination could be originated in raw material or be incorporated during the development stages of the product, coupled with inadequate hygiene practices. The general aim of this study was to evaluate the occurrence of Listeria spp. along the processing line of cheese with middle and high humidity, in three dairy subjected to different levels of sanitary survey located in Southern Brazil. From 208 samples tested Listeria spp. were isolated 7.69%, being isolated 43.75%, 25%, 31.25%, respectively, in dairy products A, B and C. From these isolates, 25% were found in raw milk, 18.75% in contact surfaces with the product, 50% in areas without contact with the product and 6.25% in the final product. L. innocua and L. monocytogenes were the only species identified between the isolates being L. innocua the prevalent species (68.7%). Among the serotypes of L. innocua, the serotype 6a was prevalent (72.7%) and between L. monocytogenes serotype 4b was prevalent (55.6%). All Listeria strains isolated from raw material and final product showed multiresistance to five antibiotics (penicillin, amoxicillin, chloramphenicol, cefaclor and clindamycin) of the fifteen antibiotics tested. Among the samples of raw material assessed microbiologically, the raw milk showed the highest levels of contamination and the highest levels found between them were > 1.1x106 MPN/mL for fecal Overall, 1.1x105 MPN/mL for fecal coliform, 4x105 CFU/ml for coagulase-positive staphylococci and Salmonella spp. in one of the samples. Among six samples of the final product, only one showed himself at odds with the existing regulatory standard, exceeding the allowed limit for ECP. Listeria spp. and L. monocytogenes were present along the lines of the three dairy processing plants, regardless of the size of the industry, highlighting the risk of contamination during food manufacturing. Therefore, it becomes necessary to introduce effective measures to reduce such contamination or improvements in the practices already adopted by the industry, because of the risks to public health. |
id |
UFPL_2fc34fcedec57c48b90d875da5d676e2 |
---|---|
oai_identifier_str |
oai:guaiaca.ufpel.edu.br:123456789/1319 |
network_acronym_str |
UFPL |
network_name_str |
Repositório Institucional da UFPel - Guaiaca |
repository_id_str |
|
spelling |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4256996A1http://lattes.cnpq.br/5231261744494804Silva, Wladimir Padilha daPrates, Denise da Fontoura2014-08-20T13:42:06Z2011-07-062014-08-20T13:42:06Z2010-12-17PRATES, Denise da Fontoura. Occurrence, characterization and serological evaluation of the profile of antibiotic resistance in Listeria spp. isolated in cheese dairy processors in southern Brazil. 2010. 98 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2010.http://repositorio.ufpel.edu.br/handle/ri/1319Listeria monocytogenes is a Gram-positive of ubiquitous natural, psychrotropic pathogen which colonizes the surfaces of equipment and utensils in food processing industries. Milk and dairy products, especially cheeses with medium and high humidity have been implicated in listeriosis cases and outbreaks. Microbial contamination could be originated in raw material or be incorporated during the development stages of the product, coupled with inadequate hygiene practices. The general aim of this study was to evaluate the occurrence of Listeria spp. along the processing line of cheese with middle and high humidity, in three dairy subjected to different levels of sanitary survey located in Southern Brazil. From 208 samples tested Listeria spp. were isolated 7.69%, being isolated 43.75%, 25%, 31.25%, respectively, in dairy products A, B and C. From these isolates, 25% were found in raw milk, 18.75% in contact surfaces with the product, 50% in areas without contact with the product and 6.25% in the final product. L. innocua and L. monocytogenes were the only species identified between the isolates being L. innocua the prevalent species (68.7%). Among the serotypes of L. innocua, the serotype 6a was prevalent (72.7%) and between L. monocytogenes serotype 4b was prevalent (55.6%). All Listeria strains isolated from raw material and final product showed multiresistance to five antibiotics (penicillin, amoxicillin, chloramphenicol, cefaclor and clindamycin) of the fifteen antibiotics tested. Among the samples of raw material assessed microbiologically, the raw milk showed the highest levels of contamination and the highest levels found between them were > 1.1x106 MPN/mL for fecal Overall, 1.1x105 MPN/mL for fecal coliform, 4x105 CFU/ml for coagulase-positive staphylococci and Salmonella spp. in one of the samples. Among six samples of the final product, only one showed himself at odds with the existing regulatory standard, exceeding the allowed limit for ECP. Listeria spp. and L. monocytogenes were present along the lines of the three dairy processing plants, regardless of the size of the industry, highlighting the risk of contamination during food manufacturing. Therefore, it becomes necessary to introduce effective measures to reduce such contamination or improvements in the practices already adopted by the industry, because of the risks to public health.Listeria monocytogenes é uma bactéria Gram-positiva de natureza ubíqua, psicrotrófica, que coloniza superfícies de equipamentos e utensílios dentro de indústrias processadoras de alimentos. Leites e derivados lácteos, em especial queijos com média e alta umidade, têm sido incriminados em casos e surtos de listeriose, por serem alimentos que favorecem o desenvolvimento microbiano, além de serem prontos para o consumo. A contaminação microbiana pode ter origem na matéria-prima, ou ser incorporada durante as etapas de elaboração do produto, associada a práticas inadequadas de higiene. O objetivo geral neste trabalho foi avaliar a ocorrência de Listeria spp., em especial de L. monocytogenes, ao longo da linha de processamento de queijos de média e alta umidade, em três laticínios, localizados na região sul do Rio Grande do Sul, submetidos a diferentes níveis de inspeção sanitária. Das 208 amostras avaliadas 7,69% apresentaram Listeria spp., sendo isoladas, 43,75%, 25%, 31,25%, respectivamente, nos laticínios A, B e C. Destes isolados, 25% foram encontrados no leite cru, 18,75% em superfícies de contato com o produto, 50% em superfícies sem contato com o produto e 6,25% no produto final. L. innocua e L. monocytogenes foram as únicas espécies identificadas entre os isolados, sendo L. innocua a espécie prevalente (68,7%). Entre os sorotipos de L. innocua, o prevalente foi 6a (72,7%) e entre L. monocytogenes o sorotipo prevalente foi 4b (55,6%). Todas as cepas de Listeria isoladas da matéria-prima e do produto final apresentaram multirresistência a cinco (penicilina, amoxilina, cloranfenicol, cefaclor e clindamicina) dos 15 antibióticos testados. Dentre as amostras de matéria-prima avaliadas microbiologicamente, as de leite cru, foram as que apresentaram os maiores níveis de contaminação, sendo que, os níveis mais elevados encontrados entre as amostras de leite cru foram: >1,1x106 NMP.mL-1 para coliformes totais, 1,1x105 NMP.mL-1 para coliformes termotolerantes, 4x105 UFC.mL-1 para estafilococos coagulase positiva (ECP) e a presença de Salmonella spp. em uma das amostras avaliadas. Entre as seis amostras de produto final, apenas uma apresentou-se em desacordo com o padrão regulamentar vigente, excedendo o limite permitido para ECP. Listeria spp. e L. monocytogenes foram isoladas ao longo das linhas de processamentos nos três laticínios, independente do porte da indústria, evidenciando risco de contaminação durante a fabricação dos dois tipos de queijo, os quais são produtos prontos para o consumo. Dessa forma, torna-se necessária a implantação de medidas eficazes para redução dessas contaminações, ou melhorias nas práticas já adotadas pelas indústrias, em virtude dos riscos à saúde pública.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em Ciência e Tecnologia AgroindustrialUFPelBRFaculdade de Agronomia Eliseu MacielListeria monocytogenesListeria spp.QueijosLaticíniosAntimicrobianosCheesesProcessing plantsCNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOSOcorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do SulOccurrence, characterization and serological evaluation of the profile of antibiotic resistance in Listeria spp. isolated in cheese dairy processors in southern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALDenise da Fontoura Prates.pdfapplication/pdf535436http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1319/1/Denise%20da%20Fontoura%20Prates.pdfc3acb08e8f12a05440096b13c688b6f2MD51open accessTEXTDenise da Fontoura Prates.pdf.txtDenise da Fontoura Prates.pdf.txtExtracted Texttext/plain197673http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1319/2/Denise%20da%20Fontoura%20Prates.pdf.txt3e9fb85c0324bfd2c57997f20a861302MD52open accessTHUMBNAILDenise da Fontoura Prates.pdf.jpgDenise da Fontoura Prates.pdf.jpgGenerated Thumbnailimage/jpeg1406http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1319/3/Denise%20da%20Fontoura%20Prates.pdf.jpg916b6432c806b434b8fbc79c4807298dMD53open access123456789/13192022-12-14 18:40:06.983open accessoai:guaiaca.ufpel.edu.br:123456789/1319Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2022-12-14T21:40:06Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Ocorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do Sul |
dc.title.alternative.eng.fl_str_mv |
Occurrence, characterization and serological evaluation of the profile of antibiotic resistance in Listeria spp. isolated in cheese dairy processors in southern Brazil |
title |
Ocorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do Sul |
spellingShingle |
Ocorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do Sul Prates, Denise da Fontoura Listeria monocytogenes Listeria spp. Queijos Laticínios Antimicrobianos Cheeses Processing plants CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
title_short |
Ocorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do Sul |
title_full |
Ocorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do Sul |
title_fullStr |
Ocorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do Sul |
title_full_unstemmed |
Ocorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do Sul |
title_sort |
Ocorrência, caracterização sorológica e avaliação do perfil de resistência a antibióticos em Listeria spp. isoladas em laticínios processadores de queijos no sul do Rio Grande do Sul |
author |
Prates, Denise da Fontoura |
author_facet |
Prates, Denise da Fontoura |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4256996A1 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/5231261744494804 |
dc.contributor.advisor1.fl_str_mv |
Silva, Wladimir Padilha da |
dc.contributor.author.fl_str_mv |
Prates, Denise da Fontoura |
contributor_str_mv |
Silva, Wladimir Padilha da |
dc.subject.por.fl_str_mv |
Listeria monocytogenes Listeria spp. Queijos Laticínios Antimicrobianos |
topic |
Listeria monocytogenes Listeria spp. Queijos Laticínios Antimicrobianos Cheeses Processing plants CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
dc.subject.eng.fl_str_mv |
Cheeses Processing plants |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::CIENCIA E TECNOLOGIA DE ALIMENTOS |
description |
Listeria monocytogenes is a Gram-positive of ubiquitous natural, psychrotropic pathogen which colonizes the surfaces of equipment and utensils in food processing industries. Milk and dairy products, especially cheeses with medium and high humidity have been implicated in listeriosis cases and outbreaks. Microbial contamination could be originated in raw material or be incorporated during the development stages of the product, coupled with inadequate hygiene practices. The general aim of this study was to evaluate the occurrence of Listeria spp. along the processing line of cheese with middle and high humidity, in three dairy subjected to different levels of sanitary survey located in Southern Brazil. From 208 samples tested Listeria spp. were isolated 7.69%, being isolated 43.75%, 25%, 31.25%, respectively, in dairy products A, B and C. From these isolates, 25% were found in raw milk, 18.75% in contact surfaces with the product, 50% in areas without contact with the product and 6.25% in the final product. L. innocua and L. monocytogenes were the only species identified between the isolates being L. innocua the prevalent species (68.7%). Among the serotypes of L. innocua, the serotype 6a was prevalent (72.7%) and between L. monocytogenes serotype 4b was prevalent (55.6%). All Listeria strains isolated from raw material and final product showed multiresistance to five antibiotics (penicillin, amoxicillin, chloramphenicol, cefaclor and clindamycin) of the fifteen antibiotics tested. Among the samples of raw material assessed microbiologically, the raw milk showed the highest levels of contamination and the highest levels found between them were > 1.1x106 MPN/mL for fecal Overall, 1.1x105 MPN/mL for fecal coliform, 4x105 CFU/ml for coagulase-positive staphylococci and Salmonella spp. in one of the samples. Among six samples of the final product, only one showed himself at odds with the existing regulatory standard, exceeding the allowed limit for ECP. Listeria spp. and L. monocytogenes were present along the lines of the three dairy processing plants, regardless of the size of the industry, highlighting the risk of contamination during food manufacturing. Therefore, it becomes necessary to introduce effective measures to reduce such contamination or improvements in the practices already adopted by the industry, because of the risks to public health. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010-12-17 |
dc.date.available.fl_str_mv |
2011-07-06 2014-08-20T13:42:06Z |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:42:06Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PRATES, Denise da Fontoura. Occurrence, characterization and serological evaluation of the profile of antibiotic resistance in Listeria spp. isolated in cheese dairy processors in southern Brazil. 2010. 98 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2010. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/1319 |
identifier_str_mv |
PRATES, Denise da Fontoura. Occurrence, characterization and serological evaluation of the profile of antibiotic resistance in Listeria spp. isolated in cheese dairy processors in southern Brazil. 2010. 98 f. Dissertação (Mestrado em Agronomia) - Universidade Federal de Pelotas, Pelotas, 2010. |
url |
http://repositorio.ufpel.edu.br/handle/ri/1319 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Ciência e Tecnologia Agroindustrial |
dc.publisher.initials.fl_str_mv |
UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Faculdade de Agronomia Eliseu Maciel |
publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFPel - Guaiaca instname:Universidade Federal de Pelotas (UFPEL) instacron:UFPEL |
instname_str |
Universidade Federal de Pelotas (UFPEL) |
instacron_str |
UFPEL |
institution |
UFPEL |
reponame_str |
Repositório Institucional da UFPel - Guaiaca |
collection |
Repositório Institucional da UFPel - Guaiaca |
bitstream.url.fl_str_mv |
http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1319/1/Denise%20da%20Fontoura%20Prates.pdf http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1319/2/Denise%20da%20Fontoura%20Prates.pdf.txt http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1319/3/Denise%20da%20Fontoura%20Prates.pdf.jpg |
bitstream.checksum.fl_str_mv |
c3acb08e8f12a05440096b13c688b6f2 3e9fb85c0324bfd2c57997f20a861302 916b6432c806b434b8fbc79c4807298d |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL) |
repository.mail.fl_str_mv |
rippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.br |
_version_ |
1801846899795296256 |