Desenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporárias
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2252 |
Resumo: | The application of a material after periodontal surgery is recommended to reduce postoperative bleeding, to protect the wound area and to avoid the formation of excessive granulation tissue. The current trend is the development of new biomaterials eugenol-free for surgical application. The aim of this study was to develop and characterize a periodontal dressing based on photopolymerizable methacrylate monomers. For this purpose, a screening was made to determine the best formulation based on the ease of handling and the use of Exothane 8 , camphorquinone, silica and quartz particles. Three groups were tested: Gperio (Group of periodontal dressing Periobond®, Dentsply); Gbar (Group of periodontal dressing Barricaid®, Caulk), which served as the reference group and Gexp (Group of experimental material). The physical-mechanical performance of the experimental composite and the commercial references Periobond® and Barricaid® (control group) were tested using in vitro tests of tensile strength (n = 15) and sorption and solubility (n = 10). Cytotoxicity (MTT colorimetric assay) and genotoxicity by micronuclei (MN) tests were performed in a cell lineage of mouse fibroblasts 3T3/NIH, in triplicate, in order to characterize the biological response of these materials. Microbiological test assess the amount of viable bacteria of Enterococcus faecalis anaerobic and aerobic and total microorganisms. Data were subjected to statistical analysis based on the model of normal distribution and equal variance One-Way ANOVA, considering the value of P<0.05 as statistically significant. There were no statistically significant differences between the median values of Gexp and Gbar, for ultimate tensile strength. Regarding the sorption there was statistical difference between Gperio and Gexp and between Gperio and Gbar (P=<0.001). There was no difference when comparing Gbar and Gexp. The solubility showed no statistical difference between groups. In general, the cytotoxic effects of the materials were time dependent according to eluate extraction periods (24h, 48h and 7 days). No statistical difference at 24h between Gexp and Gperio. At 72h, differences were observed between Gexp and Gperio (P<0.001) with Gexp showing the best results. Similar results were seen between Gbar and Gperio (P<0.001). At 7 days, statistical difference was observed between Gbar and Gperio (P<0.05). In genotoxicity test no difference was found among the groups. In microbiological assay no statistical difference was found for E. faecalis anaerobic, E. faecalis aerobic and for total microorganisms. We conclude that Gexp experienced similar behavior to the commercial samples and can be possibly used as a periodontal dressing; however, more studies are needed at pre-clinical and clinical levels |
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2014-08-20T14:30:10Z2014-01-222014-08-20T14:30:10Z2012-12-20CONCEIÇÃO, Luciana Domingues. Development of photoactivated composite for application in periodontal temporary roofing. 2012. 65 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2012.http://repositorio.ufpel.edu.br/handle/ri/2252The application of a material after periodontal surgery is recommended to reduce postoperative bleeding, to protect the wound area and to avoid the formation of excessive granulation tissue. The current trend is the development of new biomaterials eugenol-free for surgical application. The aim of this study was to develop and characterize a periodontal dressing based on photopolymerizable methacrylate monomers. For this purpose, a screening was made to determine the best formulation based on the ease of handling and the use of Exothane 8 , camphorquinone, silica and quartz particles. Three groups were tested: Gperio (Group of periodontal dressing Periobond®, Dentsply); Gbar (Group of periodontal dressing Barricaid®, Caulk), which served as the reference group and Gexp (Group of experimental material). The physical-mechanical performance of the experimental composite and the commercial references Periobond® and Barricaid® (control group) were tested using in vitro tests of tensile strength (n = 15) and sorption and solubility (n = 10). Cytotoxicity (MTT colorimetric assay) and genotoxicity by micronuclei (MN) tests were performed in a cell lineage of mouse fibroblasts 3T3/NIH, in triplicate, in order to characterize the biological response of these materials. Microbiological test assess the amount of viable bacteria of Enterococcus faecalis anaerobic and aerobic and total microorganisms. Data were subjected to statistical analysis based on the model of normal distribution and equal variance One-Way ANOVA, considering the value of P<0.05 as statistically significant. There were no statistically significant differences between the median values of Gexp and Gbar, for ultimate tensile strength. Regarding the sorption there was statistical difference between Gperio and Gexp and between Gperio and Gbar (P=<0.001). There was no difference when comparing Gbar and Gexp. The solubility showed no statistical difference between groups. In general, the cytotoxic effects of the materials were time dependent according to eluate extraction periods (24h, 48h and 7 days). No statistical difference at 24h between Gexp and Gperio. At 72h, differences were observed between Gexp and Gperio (P<0.001) with Gexp showing the best results. Similar results were seen between Gbar and Gperio (P<0.001). At 7 days, statistical difference was observed between Gbar and Gperio (P<0.05). In genotoxicity test no difference was found among the groups. In microbiological assay no statistical difference was found for E. faecalis anaerobic, E. faecalis aerobic and for total microorganisms. We conclude that Gexp experienced similar behavior to the commercial samples and can be possibly used as a periodontal dressing; however, more studies are needed at pre-clinical and clinical levelsA aplicação de um material após a cirurgia periodontal é recomendada para reduzir o sangramento pós-operatório, proteger a área da ferida e evitar a formação excessiva de tecido de granulação. A tendência atual é o desenvolvimento de novos biomateriais para aplicação cirúrgica livres de eugenol. O objetivo do presente estudo foi desenvolver e caracterizar um material cirúrgico periodontal baseado em monômeros metacrilatos fotopolimerizável com formação de polímero de alta flexibilidade. Para isso, foi realizado um screening para se determinar a melhor fórmula baseada na facilidade de manuseio do material e considerando a utilização de Exothane 8 , canforoquinona, e partículas de sílica e quartzo. Três grupos foram testados; Gperio (Grupo cimento periodontal Periobond®, Dentsply); Gbar (Grupo cimento periodontal Barricaid®, Caulk, que serviu como grupo de referência comercial) e Gexp (Grupo do material experimental). Foi avaliado o desempenho físico-mecânico do compósito experimental e das referências comerciais: Periobond® e Barricaid® (grupos controle), através dos ensaios in vitro de resistência à tração (n=15) e sorção e solubilidade (n=10). Testes de citotoxidade (ensaio colorimétrico com MTT) e de genotoxicidade através do ensaio de micronúcleos (MN) em linhagem celular de fibroblastos de camundongo 3T3/NIH, foram realizados em triplicata, com o intuito de caracterizar a resposta biológica desses materiais. Teste microbiológico para avaliar a quantidade de bactérias viáveis de Enterococcus faecalis anaeróbicos, aeróbicos e micro-organismos totais. Os dados obtidos foram submetidos ao método estatístico baseado no modelo de distribuição normal e igualdade de variância (ANOVA 1 via), considerando-se o valor de P<0,05 como estatisticamente significante. Não foram encontradas diferenças estatisticamente significantes entre os valores medianos do Gexp e Gbar, em relação à resistência máxima a tração. Em relação à sorção houve diferença estatística entre Gperio e Gexp e entre Gperio e Gbar (P<0,001). Não houve diferença estatisticamente significante quando comparamos Gbar e Gexp. Em relação à solubilidade não houve diferença estatística entre os grupos. Em geral, os efeitos citotóxicos dos materiais foram dependentes do tempo de extração do eludato (24h, 48h e 7 dias). Foi encontrada diferença estatística no tempo de 24h entre Gexp e Gperio. No tempo de 72h houve diferença entre Gexp e Gperio (P<0,001), tendo o primeiro melhor comportamento, da mesma forma entre Gbar e Gperio (P<0,001). Em 7 dias, houve diferença estatística entre Gbar e Gperio, tendo resultado de P<0,05. No teste de genotoxicidade não foi encontrada diferença estatística entre os grupos. No ensaio microbiológico não foi encontrada diferença estatística para E. faecalis anaeróbicos, E. faecalis aeróbicos ou micro-organismos totais. Concluímos que Gexp teve comportamento semelhante aos controles podendo ser utilizado como material para recobrimento de feridas cirúrgicas periodontais, sendo necessários mais estudos de fase pré-clínica e clínica.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em OdontologiaUFPelBROdontologiaMateriais dentáriosCimentos periodontaisCitotoxidadeGenotoxidadeResistência à traçãoSorção e solubilidadeEnsaio microbiológicoDental materialsPeriodontal dressingCytotoxicityGenotoxicityTensile strengthSorption and solubilityMicrobiological assayCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIADesenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporáriasDevelopment of photoactivated composite for application in periodontal temporary roofinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/8033743623803750http://lattes.cnpq.br/5001834460012280Lund, Rafael Guerrahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776008U0Piva, Evandrohttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701753T0Leite, Fábio Renato ManzolliConceição, Luciana Dominguesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALDissertacao_Luciana_Conceicao.pdfapplication/pdf1166387http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2252/1/Dissertacao_Luciana_Conceicao.pdf74e97c6a817eb6fb90d26b94051d237dMD51open accessTEXTDissertacao_Luciana_Conceicao.pdf.txtDissertacao_Luciana_Conceicao.pdf.txtExtracted Texttext/plain92652http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2252/2/Dissertacao_Luciana_Conceicao.pdf.txtfb0b4769f601dda71a7d75456878ff6cMD52open accessTHUMBNAILDissertacao_Luciana_Conceicao.pdf.jpgDissertacao_Luciana_Conceicao.pdf.jpgGenerated Thumbnailimage/jpeg1814http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2252/3/Dissertacao_Luciana_Conceicao.pdf.jpg7a7e25a84cdf27df6829975c1ee78387MD53open access123456789/22522019-09-19 09:01:54.737open accessoai:guaiaca.ufpel.edu.br:123456789/2252Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-19T12:01:54Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Desenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporárias |
dc.title.alternative.eng.fl_str_mv |
Development of photoactivated composite for application in periodontal temporary roofing |
title |
Desenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporárias |
spellingShingle |
Desenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporárias Conceição, Luciana Domingues Materiais dentários Cimentos periodontais Citotoxidade Genotoxidade Resistência à tração Sorção e solubilidade Ensaio microbiológico Dental materials Periodontal dressing Cytotoxicity Genotoxicity Tensile strength Sorption and solubility Microbiological assay CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Desenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporárias |
title_full |
Desenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporárias |
title_fullStr |
Desenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporárias |
title_full_unstemmed |
Desenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporárias |
title_sort |
Desenvolvimento de compósito fotoativado para aplicação em coberturas periodontais temporárias |
author |
Conceição, Luciana Domingues |
author_facet |
Conceição, Luciana Domingues |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/8033743623803750 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/5001834460012280 |
dc.contributor.advisor-co1.fl_str_mv |
Lund, Rafael Guerra |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776008U0 |
dc.contributor.advisor-co2.fl_str_mv |
Piva, Evandro |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701753T0 |
dc.contributor.advisor1.fl_str_mv |
Leite, Fábio Renato Manzolli |
dc.contributor.author.fl_str_mv |
Conceição, Luciana Domingues |
contributor_str_mv |
Lund, Rafael Guerra Piva, Evandro Leite, Fábio Renato Manzolli |
dc.subject.por.fl_str_mv |
Materiais dentários Cimentos periodontais Citotoxidade Genotoxidade Resistência à tração Sorção e solubilidade Ensaio microbiológico |
topic |
Materiais dentários Cimentos periodontais Citotoxidade Genotoxidade Resistência à tração Sorção e solubilidade Ensaio microbiológico Dental materials Periodontal dressing Cytotoxicity Genotoxicity Tensile strength Sorption and solubility Microbiological assay CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Dental materials Periodontal dressing Cytotoxicity Genotoxicity Tensile strength Sorption and solubility Microbiological assay |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
The application of a material after periodontal surgery is recommended to reduce postoperative bleeding, to protect the wound area and to avoid the formation of excessive granulation tissue. The current trend is the development of new biomaterials eugenol-free for surgical application. The aim of this study was to develop and characterize a periodontal dressing based on photopolymerizable methacrylate monomers. For this purpose, a screening was made to determine the best formulation based on the ease of handling and the use of Exothane 8 , camphorquinone, silica and quartz particles. Three groups were tested: Gperio (Group of periodontal dressing Periobond®, Dentsply); Gbar (Group of periodontal dressing Barricaid®, Caulk), which served as the reference group and Gexp (Group of experimental material). The physical-mechanical performance of the experimental composite and the commercial references Periobond® and Barricaid® (control group) were tested using in vitro tests of tensile strength (n = 15) and sorption and solubility (n = 10). Cytotoxicity (MTT colorimetric assay) and genotoxicity by micronuclei (MN) tests were performed in a cell lineage of mouse fibroblasts 3T3/NIH, in triplicate, in order to characterize the biological response of these materials. Microbiological test assess the amount of viable bacteria of Enterococcus faecalis anaerobic and aerobic and total microorganisms. Data were subjected to statistical analysis based on the model of normal distribution and equal variance One-Way ANOVA, considering the value of P<0.05 as statistically significant. There were no statistically significant differences between the median values of Gexp and Gbar, for ultimate tensile strength. Regarding the sorption there was statistical difference between Gperio and Gexp and between Gperio and Gbar (P=<0.001). There was no difference when comparing Gbar and Gexp. The solubility showed no statistical difference between groups. In general, the cytotoxic effects of the materials were time dependent according to eluate extraction periods (24h, 48h and 7 days). No statistical difference at 24h between Gexp and Gperio. At 72h, differences were observed between Gexp and Gperio (P<0.001) with Gexp showing the best results. Similar results were seen between Gbar and Gperio (P<0.001). At 7 days, statistical difference was observed between Gbar and Gperio (P<0.05). In genotoxicity test no difference was found among the groups. In microbiological assay no statistical difference was found for E. faecalis anaerobic, E. faecalis aerobic and for total microorganisms. We conclude that Gexp experienced similar behavior to the commercial samples and can be possibly used as a periodontal dressing; however, more studies are needed at pre-clinical and clinical levels |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-12-20 |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:30:10Z |
dc.date.available.fl_str_mv |
2014-01-22 2014-08-20T14:30:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CONCEIÇÃO, Luciana Domingues. Development of photoactivated composite for application in periodontal temporary roofing. 2012. 65 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2252 |
identifier_str_mv |
CONCEIÇÃO, Luciana Domingues. Development of photoactivated composite for application in periodontal temporary roofing. 2012. 65 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2252 |
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por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Odontologia |
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UFPel |
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BR |
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Odontologia |
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Universidade Federal de Pelotas |
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