Caracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do Brasil
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://guaiaca.ufpel.edu.br/handle/123456789/1204 |
Resumo: | Bovine tuberculosis (BTB) caused by Mycobacterium bovis is an infectious disease of zoonotic which mainly affects cattle and buffaloes, and cause economic losses in the production of meat and milk in many countries. In Brazil, the official rates are at 1.3% of the national herd infected, corresponding to approximately 2.5 million animals. To contribute to the control of BTB, many molecular techniques have been developed in order to differentiate isolates and establish epidemiological correlations between them. Among these techniques, the most used are the Restriction Fragment Lenght Polymorphism (RFLP), Polymorphic Guanine-cytosine-Rich Sequence [1], Spoligotyping [2], Mycobacterial Interspersed Repetitive Units - Variable Number of Tandem Repeat (MIRU-VNTR) and Exact Tandem Repeat [3]. The spoligotyping and MIRU-VNTR are today considered standard methods of molecular techniques for typing of M. bovis. Both are based on PCR techniques that evaluate polymorphism in the number of repeat sequences present in the genome. Taking into account that the molecular characterization enables to increase our knowledge on the epidemiology of M. bovis and in tuberculosis control, this study aimed at the molecular characterization of isolates of M. bovis in the north and south of the Brazil, by isolating agent, identification of M. bovis by amplification of the region RD7 Multiplex PCR and genotyping using the techniques of spoligotyping, MIRU-VNTR and ETR. Nine-nine lymph nodes were collected from beef cattle in the north of the region and 162 dairy cattle in southern Brazil, and only 10 samples were positive for M. bovis in the north and 85 in the south region. Through analysis of molecular combination of spoligotyping and VNTR was possible to identify different genotypic patterns in the regions studied demonstrating the genetic diversity of M. bovis in our country. |
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2014-08-20T13:32:45Z2013-04-292014-08-20T13:32:45Z2012-11-30RAMOS, Daniela Fernandes. Molecular characterization of Mycobacterium bovis isolates from cattle from different regions of Brazil. 2012. 108 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2012.http://guaiaca.ufpel.edu.br/handle/123456789/1204Bovine tuberculosis (BTB) caused by Mycobacterium bovis is an infectious disease of zoonotic which mainly affects cattle and buffaloes, and cause economic losses in the production of meat and milk in many countries. In Brazil, the official rates are at 1.3% of the national herd infected, corresponding to approximately 2.5 million animals. To contribute to the control of BTB, many molecular techniques have been developed in order to differentiate isolates and establish epidemiological correlations between them. Among these techniques, the most used are the Restriction Fragment Lenght Polymorphism (RFLP), Polymorphic Guanine-cytosine-Rich Sequence [1], Spoligotyping [2], Mycobacterial Interspersed Repetitive Units - Variable Number of Tandem Repeat (MIRU-VNTR) and Exact Tandem Repeat [3]. The spoligotyping and MIRU-VNTR are today considered standard methods of molecular techniques for typing of M. bovis. Both are based on PCR techniques that evaluate polymorphism in the number of repeat sequences present in the genome. Taking into account that the molecular characterization enables to increase our knowledge on the epidemiology of M. bovis and in tuberculosis control, this study aimed at the molecular characterization of isolates of M. bovis in the north and south of the Brazil, by isolating agent, identification of M. bovis by amplification of the region RD7 Multiplex PCR and genotyping using the techniques of spoligotyping, MIRU-VNTR and ETR. Nine-nine lymph nodes were collected from beef cattle in the north of the region and 162 dairy cattle in southern Brazil, and only 10 samples were positive for M. bovis in the north and 85 in the south region. Through analysis of molecular combination of spoligotyping and VNTR was possible to identify different genotypic patterns in the regions studied demonstrating the genetic diversity of M. bovis in our country.A tuberculose bovina (BTB) causada pelo Mycobacterium bovis é uma doença infecto-contagiosa de caráter zoonótico que acomete principalmente bovinos e bubalinos, e causa prejuízos econômicos na produção de carne e leite em muitos países. No Brasil, os índices oficiais estão em 1,3% do rebanho nacional infectado, correspondendo a, aproximadamente, 2,5 milhões de animais. Visando contribuir para o controle da BTB, muitas técnicas moleculares vêm sendo desenvolvidas com o objetivo de diferenciar isolados e estabelecer correlações epidemiológicas entre eles. Dentre essas técnicas, as mais usadas são o Restriction Fragment Lenght Polymorphism (RFLP), Polymorphic Guanine-cytosine-Rich Sequence [1], Spoligotyping [2], Mycobacterial Interspersed Repetitive Units - Variable Number of Tandem Repeat (MIRU-VNTR) e Exact Tandem Repeat [3]. O spoligotyping e MIRU-VNTR são, hoje, considerados métodos padrões de técnicas moleculares para tipagem de M. bovis. Ambas são técnicas baseadas em PCR que avaliam o polimorfismo do número de sequências repetidas presentes no genoma. Levando em conta que a caracterização molecular possibilita aumentar nosso conhecimento na epidemiologia do M. bovis e no controle da tuberculose, este trabalho teve como objetivo a caracterização molecular de isolados de M. bovis da região norte e sul do Brasil, através do isolamento do agente, identificação de M. bovis pela amplificação da região RD7 por PCR Multiplex e genotipagem usando as técnicas de spoligotyping, MIRU-VNTR e ETR. Foram coletadas 99 linfonodos de bovinos de corte da região norte e 162 de bovinos de leite da região sul do Brasil, sendo que apenas 10 amostras foram positivas para M. bovis no norte e 85 na região sul. Através da análise molecular da combinação de spoligotyping e VNTR foi possível identificar diferentes padrões genotípicos nas regiões estudas demonstrando a diversidade genética de M. bovis no nosso país.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em BiotecnologiaUFPelBRBiotecnologiaMycobacterium bovisTuberculosisGenotypingMycobacterium bovisTuberculoseGenotipagemCNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIACaracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do BrasilMolecular characterization of Mycobacterium bovis isolates from cattle from different regions of Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesishttp://lattes.cnpq.br/7999751854162355http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723107D9Dellagostin, Odir AntônioRamos, Daniela Fernandesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALtese_daniela_fernandes_ramos.pdfapplication/pdf1004504http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1204/1/tese_daniela_fernandes_ramos.pdfd8fd2e285277928c8af06e08c3527471MD51open accessTEXTtese_daniela_fernandes_ramos.pdf.txttese_daniela_fernandes_ramos.pdf.txtExtracted Texttext/plain158026http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1204/2/tese_daniela_fernandes_ramos.pdf.txtd557b7e0ad614f9bded7f1db99ffa03aMD52open accessTHUMBNAILtese_daniela_fernandes_ramos.pdf.jpgtese_daniela_fernandes_ramos.pdf.jpgGenerated Thumbnailimage/jpeg1718http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/1204/3/tese_daniela_fernandes_ramos.pdf.jpg6fbe6aa2d3a2b764407ffcd135064af1MD53open access123456789/12042019-08-23 09:36:29.963open accessoai:guaiaca.ufpel.edu.br:123456789/1204Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-23T12:36:29Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Caracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do Brasil |
dc.title.alternative.eng.fl_str_mv |
Molecular characterization of Mycobacterium bovis isolates from cattle from different regions of Brazil |
title |
Caracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do Brasil |
spellingShingle |
Caracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do Brasil Ramos, Daniela Fernandes Mycobacterium bovis Tuberculosis Genotyping Mycobacterium bovis Tuberculose Genotipagem CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIA |
title_short |
Caracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do Brasil |
title_full |
Caracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do Brasil |
title_fullStr |
Caracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do Brasil |
title_full_unstemmed |
Caracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do Brasil |
title_sort |
Caracterização molecular de isolados de Mycobacterium bovis de rebanhos de diferentes regiões do Brasil |
author |
Ramos, Daniela Fernandes |
author_facet |
Ramos, Daniela Fernandes |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/7999751854162355 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4723107D9 |
dc.contributor.advisor1.fl_str_mv |
Dellagostin, Odir Antônio |
dc.contributor.author.fl_str_mv |
Ramos, Daniela Fernandes |
contributor_str_mv |
Dellagostin, Odir Antônio |
dc.subject.eng.fl_str_mv |
Mycobacterium bovis Tuberculosis Genotyping |
topic |
Mycobacterium bovis Tuberculosis Genotyping Mycobacterium bovis Tuberculose Genotipagem CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIA |
dc.subject.por.fl_str_mv |
Mycobacterium bovis Tuberculose Genotipagem |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA::BIOLOGIA E FISIOLOGIA DOS MICROORGANISMOS::BACTEROLOGIA |
description |
Bovine tuberculosis (BTB) caused by Mycobacterium bovis is an infectious disease of zoonotic which mainly affects cattle and buffaloes, and cause economic losses in the production of meat and milk in many countries. In Brazil, the official rates are at 1.3% of the national herd infected, corresponding to approximately 2.5 million animals. To contribute to the control of BTB, many molecular techniques have been developed in order to differentiate isolates and establish epidemiological correlations between them. Among these techniques, the most used are the Restriction Fragment Lenght Polymorphism (RFLP), Polymorphic Guanine-cytosine-Rich Sequence [1], Spoligotyping [2], Mycobacterial Interspersed Repetitive Units - Variable Number of Tandem Repeat (MIRU-VNTR) and Exact Tandem Repeat [3]. The spoligotyping and MIRU-VNTR are today considered standard methods of molecular techniques for typing of M. bovis. Both are based on PCR techniques that evaluate polymorphism in the number of repeat sequences present in the genome. Taking into account that the molecular characterization enables to increase our knowledge on the epidemiology of M. bovis and in tuberculosis control, this study aimed at the molecular characterization of isolates of M. bovis in the north and south of the Brazil, by isolating agent, identification of M. bovis by amplification of the region RD7 Multiplex PCR and genotyping using the techniques of spoligotyping, MIRU-VNTR and ETR. Nine-nine lymph nodes were collected from beef cattle in the north of the region and 162 dairy cattle in southern Brazil, and only 10 samples were positive for M. bovis in the north and 85 in the south region. Through analysis of molecular combination of spoligotyping and VNTR was possible to identify different genotypic patterns in the regions studied demonstrating the genetic diversity of M. bovis in our country. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-11-30 |
dc.date.available.fl_str_mv |
2013-04-29 2014-08-20T13:32:45Z |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:32:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RAMOS, Daniela Fernandes. Molecular characterization of Mycobacterium bovis isolates from cattle from different regions of Brazil. 2012. 108 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
dc.identifier.uri.fl_str_mv |
http://guaiaca.ufpel.edu.br/handle/123456789/1204 |
identifier_str_mv |
RAMOS, Daniela Fernandes. Molecular characterization of Mycobacterium bovis isolates from cattle from different regions of Brazil. 2012. 108 f. Tese (Doutorado em Biotecnologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
url |
http://guaiaca.ufpel.edu.br/handle/123456789/1204 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Pelotas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Biotecnologia |
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UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Biotecnologia |
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Universidade Federal de Pelotas |
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