Efeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de prótese
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2288 |
Resumo: | Candidiasis is the most common fungal infection in denture wearers. The association between Candida and denture base is directly related to the efficiency of this micro-organism to adhere and colonize these surfaces, which is an essential initial step for the development of denture-related stomatitis. Additionally, the use of denture liners has increased, with these materials more prone to microbial adhesion when compared with acrylic resin due to its higher surface roughness. These materials have demonstrated capacity to interact with oral micro-organisms.This important problem is the reason why several attempts have been made to develop biomaterials containing antifungal. Thus, the aim of this study was to investigate the potential antifungal activity of triazine added to acrylic resin and liners used in prosthodontics using microcosms biofilm model with saliva derived from patients with or without candidiasis, verifying its effectiveness in reducing microbial adherence and consequently reduce the incidence of Candida infections. The mechanical properties of the tested materials after the incorporation of triazine were also evaluated. We considered four commercially available materials (a microwave-cured acrylic resin and two soft denture liners and one hard denture liner) to which were added different concentrations of triazine (0; 2.5; 5.0 and 10%). The results indicated that no difference were found among triazine concentrations in acrylic resin flexural strength (p= 0.059). Triazine addition did not result in surface roughness changes, although all denture liners presented higher surface roughness compared with acrylic resin, while in general, SoftConfort presented the highest roughness (p<0.001). In general, 5 and 10% triazine addition resulted in more soluble materials. All materials presented increased water sorption with the addition of triazine (p<0.05), except for CoeSoft, where no change was observed. Candidiasis patient saliva presented higher counts of total micro-organisms (p= 0.0294) and total streptococci (p = 0.0008). Regarding materials, total streptococci, total micro-organisms and Candida counts were higher in CoeSoft (p<0.0001). Candida species counts was the same for both saliva donors (p>0.05). The addition of triazine did not result in decrease of total micro-organisms, total streptococci or Candida species in all materials tested (p>0.05). In conclusion, the addition of triazine to acrylic resin and denture liners in a complex biofilm model did not modify the development of biofilms in both patients with and without candidiasis |
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2014-08-20T14:30:16Z2014-02-072014-08-20T14:30:16Z2011-04-01MORAES, Aline Pinheiro de. Antimicrobial activity of triazine added to denture materials on saliva-derived microcosms: microbiological efficacy and mechanical properties. 2011. 83 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2011.http://repositorio.ufpel.edu.br/handle/ri/2288Candidiasis is the most common fungal infection in denture wearers. The association between Candida and denture base is directly related to the efficiency of this micro-organism to adhere and colonize these surfaces, which is an essential initial step for the development of denture-related stomatitis. Additionally, the use of denture liners has increased, with these materials more prone to microbial adhesion when compared with acrylic resin due to its higher surface roughness. These materials have demonstrated capacity to interact with oral micro-organisms.This important problem is the reason why several attempts have been made to develop biomaterials containing antifungal. Thus, the aim of this study was to investigate the potential antifungal activity of triazine added to acrylic resin and liners used in prosthodontics using microcosms biofilm model with saliva derived from patients with or without candidiasis, verifying its effectiveness in reducing microbial adherence and consequently reduce the incidence of Candida infections. The mechanical properties of the tested materials after the incorporation of triazine were also evaluated. We considered four commercially available materials (a microwave-cured acrylic resin and two soft denture liners and one hard denture liner) to which were added different concentrations of triazine (0; 2.5; 5.0 and 10%). The results indicated that no difference were found among triazine concentrations in acrylic resin flexural strength (p= 0.059). Triazine addition did not result in surface roughness changes, although all denture liners presented higher surface roughness compared with acrylic resin, while in general, SoftConfort presented the highest roughness (p<0.001). In general, 5 and 10% triazine addition resulted in more soluble materials. All materials presented increased water sorption with the addition of triazine (p<0.05), except for CoeSoft, where no change was observed. Candidiasis patient saliva presented higher counts of total micro-organisms (p= 0.0294) and total streptococci (p = 0.0008). Regarding materials, total streptococci, total micro-organisms and Candida counts were higher in CoeSoft (p<0.0001). Candida species counts was the same for both saliva donors (p>0.05). The addition of triazine did not result in decrease of total micro-organisms, total streptococci or Candida species in all materials tested (p>0.05). In conclusion, the addition of triazine to acrylic resin and denture liners in a complex biofilm model did not modify the development of biofilms in both patients with and without candidiasisA candidíase é a infecção oral fúngica mais comum em usuários de próteses. A associação entre o microrganismo Candida e as bases das próteses está diretamente relacionada à eficiência deste microrganismo em aderir e colonizar estas superfícies, sendo esta a etapa inicial essencial para o desenvolvimento da estomatite induzida por prótese. Associado a isto, o uso de reembasadores para próteses tem aumentado, sendo estes materiais mais propensos à adesão microbiana quando comparados a resina acrílica devido a sua maior rugosidade de superfície. Além disso, estes materiais tem demonstrado capacidade de interagir com microrganismos bucais. Este importante problema tem levado a tentativa de desenvolvimento de diversos materiais contendo agentes antifúngicos. Assim, o objetivo deste estudo foi investigar o potencial antifúngico da triazina adicionada à resina acrílica e reembasadores utilizados em prótese dentária, através de um modelo de biofilme de microcosmos, com salivas derivadas de pacientes com ou sem candidíase, verificando sua efetividade em reduzir a adesão microbiana e, consequentemente, diminuir a incidência de infecções por Candida. Também avaliaram-se as propriedades mecânicas dos materiais testados após a incorporação da triazina. Foram considerados quatro materiais comercialmente disponíveis (uma resina acrílica ativada por energia de micro-ondas, dois reembasadores resilientes e um reembasador permanente), aos quais foram adicionadas diferentes concentrações de triazina (0; 2,5; 5,0 e 10%). Os resultados indicaram que não houve diferença (p = 0,059) entre as concentrações de triazina para a resina acrílica de micro-ondas no teste de resistência à flexão. Em relação a rugosidade de superfície, embora a adição de triazina não tenha levado ao aumento da rugosidade, os reembasadores apresentaram maior rugosidade que a resina acrílica, com o SoftConfort apresentando, em geral, a maior rugosidade (p<0,001). De maneira geral, quando adicionada triazina a 5,0 e 10%, todos os materiais tornaram-se mais solúveis e apresentaram aumento de sorção de água (p<0,05), a exceção do CoeSoft, que não mostrou diferença com a adição do composto químico. Houve diferença entre salivas, sendo que aquela proveniente de individuo com candidíase apresentou maiores contagens de microrganismos totais (p = 0,0294) e estreptococos totais (p = 0,0008). Em relação aos materiais, a contagem de estreptococos, microrganismos totais e espécies de Candida foi maior no CoeSoft (p<0,0001). Entretanto, a contagem de espécies de Candida foi igual entre os doadores (p>0,05). A adição de triazina não resultou em diminuição de microrganismos totais, estreptococos totais ou espécies de Candida em nenhum dos materiais testados (p>0,05). Como conclusão, a adição de triazina a resina acrílica e reembasadores utilizados para confecção de base e reembasamento de próteses em um modelo complexo de biofilme não modificou o desenvolvimento de biofilmes em ambos os pacientes com ou sem candidíaseapplication/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em OdontologiaUFPelBROdontologiaCandidíaseResina acrílicaReembasadores protéticosBiofilmeTriazinaCandidiasisAcrylic resinDenture linersBiofilmTriazineCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAEfeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de próteseAntimicrobial activity of triazine added to denture materials on saliva-derived microcosms: microbiological efficacy and mechanical propertiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/7506038014417147http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4730122J7Boscato, Noélihttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798004H7Cenci, Tatiana PereiraMoraes, Aline Pinheiro deinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALDissertacao_Aline Pinheiro_Moraes.pdfapplication/pdf846337http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2288/1/Dissertacao_Aline%20Pinheiro_Moraes.pdf66b01044077d89e98213868cd5b7582aMD51open accessTEXTDissertacao_Aline Pinheiro_Moraes.pdf.txtDissertacao_Aline Pinheiro_Moraes.pdf.txtExtracted Texttext/plain121054http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2288/2/Dissertacao_Aline%20Pinheiro_Moraes.pdf.txtcad387a9dbd248cb046b84e24dcf8706MD52open accessTHUMBNAILDissertacao_Aline Pinheiro_Moraes.pdf.jpgDissertacao_Aline Pinheiro_Moraes.pdf.jpgGenerated Thumbnailimage/jpeg1851http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2288/3/Dissertacao_Aline%20Pinheiro_Moraes.pdf.jpgc9a19015dfd750b257a9430ae27fc55cMD53open access123456789/22882019-09-25 11:50:37.403open accessoai:guaiaca.ufpel.edu.br:123456789/2288Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-25T14:50:37Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Efeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de prótese |
dc.title.alternative.eng.fl_str_mv |
Antimicrobial activity of triazine added to denture materials on saliva-derived microcosms: microbiological efficacy and mechanical properties |
title |
Efeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de prótese |
spellingShingle |
Efeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de prótese Moraes, Aline Pinheiro de Candidíase Resina acrílica Reembasadores protéticos Biofilme Triazina Candidiasis Acrylic resin Denture liners Biofilm Triazine CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Efeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de prótese |
title_full |
Efeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de prótese |
title_fullStr |
Efeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de prótese |
title_full_unstemmed |
Efeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de prótese |
title_sort |
Efeito da triazina nas propriedades mecânicas e formação de biofilme em resina acrílica e reembasadores de prótese |
author |
Moraes, Aline Pinheiro de |
author_facet |
Moraes, Aline Pinheiro de |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/7506038014417147 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4730122J7 |
dc.contributor.advisor-co1.fl_str_mv |
Boscato, Noéli |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4798004H7 |
dc.contributor.advisor1.fl_str_mv |
Cenci, Tatiana Pereira |
dc.contributor.author.fl_str_mv |
Moraes, Aline Pinheiro de |
contributor_str_mv |
Boscato, Noéli Cenci, Tatiana Pereira |
dc.subject.por.fl_str_mv |
Candidíase Resina acrílica Reembasadores protéticos Biofilme Triazina |
topic |
Candidíase Resina acrílica Reembasadores protéticos Biofilme Triazina Candidiasis Acrylic resin Denture liners Biofilm Triazine CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Candidiasis Acrylic resin Denture liners Biofilm Triazine |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Candidiasis is the most common fungal infection in denture wearers. The association between Candida and denture base is directly related to the efficiency of this micro-organism to adhere and colonize these surfaces, which is an essential initial step for the development of denture-related stomatitis. Additionally, the use of denture liners has increased, with these materials more prone to microbial adhesion when compared with acrylic resin due to its higher surface roughness. These materials have demonstrated capacity to interact with oral micro-organisms.This important problem is the reason why several attempts have been made to develop biomaterials containing antifungal. Thus, the aim of this study was to investigate the potential antifungal activity of triazine added to acrylic resin and liners used in prosthodontics using microcosms biofilm model with saliva derived from patients with or without candidiasis, verifying its effectiveness in reducing microbial adherence and consequently reduce the incidence of Candida infections. The mechanical properties of the tested materials after the incorporation of triazine were also evaluated. We considered four commercially available materials (a microwave-cured acrylic resin and two soft denture liners and one hard denture liner) to which were added different concentrations of triazine (0; 2.5; 5.0 and 10%). The results indicated that no difference were found among triazine concentrations in acrylic resin flexural strength (p= 0.059). Triazine addition did not result in surface roughness changes, although all denture liners presented higher surface roughness compared with acrylic resin, while in general, SoftConfort presented the highest roughness (p<0.001). In general, 5 and 10% triazine addition resulted in more soluble materials. All materials presented increased water sorption with the addition of triazine (p<0.05), except for CoeSoft, where no change was observed. Candidiasis patient saliva presented higher counts of total micro-organisms (p= 0.0294) and total streptococci (p = 0.0008). Regarding materials, total streptococci, total micro-organisms and Candida counts were higher in CoeSoft (p<0.0001). Candida species counts was the same for both saliva donors (p>0.05). The addition of triazine did not result in decrease of total micro-organisms, total streptococci or Candida species in all materials tested (p>0.05). In conclusion, the addition of triazine to acrylic resin and denture liners in a complex biofilm model did not modify the development of biofilms in both patients with and without candidiasis |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011-04-01 |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:30:16Z |
dc.date.available.fl_str_mv |
2014-02-07 2014-08-20T14:30:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MORAES, Aline Pinheiro de. Antimicrobial activity of triazine added to denture materials on saliva-derived microcosms: microbiological efficacy and mechanical properties. 2011. 83 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2288 |
identifier_str_mv |
MORAES, Aline Pinheiro de. Antimicrobial activity of triazine added to denture materials on saliva-derived microcosms: microbiological efficacy and mechanical properties. 2011. 83 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2011. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2288 |
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por |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Odontologia |
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UFPel |
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BR |
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Odontologia |
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Universidade Federal de Pelotas |
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