Alterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2057 |
Resumo: | This study aimed to evaluate the radiosensitivity of castor bean seeds cultivar IAC80 subjected to different doses of cobalt60 gamma radiation. Firstly, the mechanical removal of the caruncle seeds was performed, after being pre-soaked for 24 hours and irradiated with cobalt60 gamma radiation (0, 50, 100, 150 and 200Gy) and, then, sown in polystyrene trays containing soil. Parameters such as electrical conductivity, emergence index and emergence rate, growth parameters and analysis of antioxidant enzymes (SOD, CAT and APX) measured/assessed in leaves and roots of castor bean plantlets /seedlings were evaluated. The results were submitted to ANOVA and the averages compared through Turkey test (5%). The electrical conductivity was not affected by the radiation doses used. The results showed that gamma radiation negatively affected plantlets/seedlings development, given that the dose of 200Gy significantly reduced emergence and speed of emergence index. The growth parameters showed a reduction in the plantlets size with the increase of radiation doses. The activities of antioxidant enzymes, superoxide dismutase showed a significant difference only at a dose of 200Gy to the leaves, not being significant to the roots. The activity of the enzyme ascorbate peroxidase showed increased activity in the leaves as the radiation doses were also increased. The activity of catalase showed no significant difference when exposed to different doses of gamma radiation in both leaves and roots. From the foregoing it can be concluded that the doses of Co60 gamma radiation used in this study were shown to have a negative effect on the morphological and physiological characteristics evaluated in this work. Regarding the activity of antioxidant enzymes (SOD and APX), the shoots of seedlings of castor bean was more sensitive to gamma radiation Co60 used. |
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2014-08-20T13:59:15Z2012-03-272014-08-20T13:59:15Z2012-03-01LOPES, Amanda Moreira. Morphophysiological and biochemical changes in castor bean seeds (Ricinus communis L.) subjected to gamma Radiation Cobalt-60.. 2012. 58 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2012.http://repositorio.ufpel.edu.br/handle/ri/2057This study aimed to evaluate the radiosensitivity of castor bean seeds cultivar IAC80 subjected to different doses of cobalt60 gamma radiation. Firstly, the mechanical removal of the caruncle seeds was performed, after being pre-soaked for 24 hours and irradiated with cobalt60 gamma radiation (0, 50, 100, 150 and 200Gy) and, then, sown in polystyrene trays containing soil. Parameters such as electrical conductivity, emergence index and emergence rate, growth parameters and analysis of antioxidant enzymes (SOD, CAT and APX) measured/assessed in leaves and roots of castor bean plantlets /seedlings were evaluated. The results were submitted to ANOVA and the averages compared through Turkey test (5%). The electrical conductivity was not affected by the radiation doses used. The results showed that gamma radiation negatively affected plantlets/seedlings development, given that the dose of 200Gy significantly reduced emergence and speed of emergence index. The growth parameters showed a reduction in the plantlets size with the increase of radiation doses. The activities of antioxidant enzymes, superoxide dismutase showed a significant difference only at a dose of 200Gy to the leaves, not being significant to the roots. The activity of the enzyme ascorbate peroxidase showed increased activity in the leaves as the radiation doses were also increased. The activity of catalase showed no significant difference when exposed to different doses of gamma radiation in both leaves and roots. From the foregoing it can be concluded that the doses of Co60 gamma radiation used in this study were shown to have a negative effect on the morphological and physiological characteristics evaluated in this work. Regarding the activity of antioxidant enzymes (SOD and APX), the shoots of seedlings of castor bean was more sensitive to gamma radiation Co60 used.O presente estudo teve por objetivo avaliar a radiossensibilidade de sementes de mamona cultivar IAC80 submetida a diferentes doses de radiação gama cobalto60. Primeiramente foi feita a remoção mecânica da carúncula das sementes, foram préembebidas por 24h em água destilada e irradiadas com radiação gama cobalto60 (0, 50, 100, 150 e 200 Gy) e posteriormente semeadas em bandejas de isopor contendo solo. Foram feitos testes de condutividade elétrica, emergência e índice de velocidade de emergência, análises de crescimento e análise das enzimas antioxidantes (SOD, CAT e APX) avaliadas nas folhas e nas raízes de plântulas de mamona. Os resultados foram submetidos à análise de variância e as médias comparadas pelo teste de Tukey (5%). A condutividade elétrica não foi afetada pelas doses de radiação utilizadas. Os resultados mostraram que a radiação gama afetou negativamente o desenvolvimento das plântulas, sendo que a dose de 200Gy reduziu significativamente a emergência e o índice de velocidade de emergência. As características morfofsiológicas apresentaram redução do tamanho das plântulas conforme o aumento das doses de radiação. Na atividade das enzimas antioxidantes, a superóxido dismutase apresentou diferença significativa apenas na dose de 200Gy, para as folhas, não sendo significativa nas raízes. A atividade da enzima ascorbato peroxidase mostrou aumento da atividade nas folhas conforme o aumento da dose de radiação. Já a atividade da catalase não mostrou diferença significativa quando exposta a diferentes doses de radiação gama tanto em folhas quanto em raízes. A partir do exposto pode-se concluir que as doses de radiação gama Co60 utilizadas neste estudo mostraram ter um efeito negativo sobre as características morfofisiológicas avaliadas neste trabalho. Quanto à atividade das enzimas antioxidantes (SOD e APX), a parte aérea das plântulas de mamona se mostrou mais sensível às doses de radiação gama Co60 utilizadas.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em Fisiologia VegetalUFPelBRBiologiaEnzimas antioxidantesIAC 80RadiossensibilidadeMamoneiraAntioxidant enzymesIAC 80RadiosensitivityCNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETALAlterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60Morphophysiological and biochemical changes in castor bean seeds (Ricinus communis L.) subjected to gamma Radiation Cobalt-60.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/0162916213005611http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784294D6Deuner, SidneiBobrowski, Vera LuciaLopes, Amanda Moreirainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_amanda_moreira_lopes.pdfapplication/pdf1115061http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2057/1/dissertacao_amanda_moreira_lopes.pdf4f2c552de4e1f1eddfe62430e67bdfacMD51open accessTEXTdissertacao_amanda_moreira_lopes.pdf.txtdissertacao_amanda_moreira_lopes.pdf.txtExtracted Texttext/plain90706http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2057/2/dissertacao_amanda_moreira_lopes.pdf.txte468e613ea1aa0456b5d1595e1fe9ba1MD52open accessTHUMBNAILdissertacao_amanda_moreira_lopes.pdf.jpgdissertacao_amanda_moreira_lopes.pdf.jpgGenerated Thumbnailimage/jpeg1246http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2057/3/dissertacao_amanda_moreira_lopes.pdf.jpg6afb965526d7ee86d54d133097c65d4aMD53open access123456789/20572019-08-19 12:06:47.31open accessoai:guaiaca.ufpel.edu.br:123456789/2057Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-08-19T15:06:47Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Alterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60 |
dc.title.alternative.eng.fl_str_mv |
Morphophysiological and biochemical changes in castor bean seeds (Ricinus communis L.) subjected to gamma Radiation Cobalt-60. |
title |
Alterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60 |
spellingShingle |
Alterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60 Lopes, Amanda Moreira Enzimas antioxidantes IAC 80 Radiossensibilidade Mamoneira Antioxidant enzymes IAC 80 Radiosensitivity CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
title_short |
Alterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60 |
title_full |
Alterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60 |
title_fullStr |
Alterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60 |
title_full_unstemmed |
Alterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60 |
title_sort |
Alterações morfofisiológicas e bioquímicas em sementes de mamona (Ricinus communis L.) submetidas à Radiação gama Cobalto60 |
author |
Lopes, Amanda Moreira |
author_facet |
Lopes, Amanda Moreira |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/0162916213005611 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784294D6 |
dc.contributor.advisor-co1.fl_str_mv |
Deuner, Sidnei |
dc.contributor.advisor1.fl_str_mv |
Bobrowski, Vera Lucia |
dc.contributor.author.fl_str_mv |
Lopes, Amanda Moreira |
contributor_str_mv |
Deuner, Sidnei Bobrowski, Vera Lucia |
dc.subject.por.fl_str_mv |
Enzimas antioxidantes IAC 80 Radiossensibilidade Mamoneira |
topic |
Enzimas antioxidantes IAC 80 Radiossensibilidade Mamoneira Antioxidant enzymes IAC 80 Radiosensitivity CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
dc.subject.eng.fl_str_mv |
Antioxidant enzymes IAC 80 Radiosensitivity |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
description |
This study aimed to evaluate the radiosensitivity of castor bean seeds cultivar IAC80 subjected to different doses of cobalt60 gamma radiation. Firstly, the mechanical removal of the caruncle seeds was performed, after being pre-soaked for 24 hours and irradiated with cobalt60 gamma radiation (0, 50, 100, 150 and 200Gy) and, then, sown in polystyrene trays containing soil. Parameters such as electrical conductivity, emergence index and emergence rate, growth parameters and analysis of antioxidant enzymes (SOD, CAT and APX) measured/assessed in leaves and roots of castor bean plantlets /seedlings were evaluated. The results were submitted to ANOVA and the averages compared through Turkey test (5%). The electrical conductivity was not affected by the radiation doses used. The results showed that gamma radiation negatively affected plantlets/seedlings development, given that the dose of 200Gy significantly reduced emergence and speed of emergence index. The growth parameters showed a reduction in the plantlets size with the increase of radiation doses. The activities of antioxidant enzymes, superoxide dismutase showed a significant difference only at a dose of 200Gy to the leaves, not being significant to the roots. The activity of the enzyme ascorbate peroxidase showed increased activity in the leaves as the radiation doses were also increased. The activity of catalase showed no significant difference when exposed to different doses of gamma radiation in both leaves and roots. From the foregoing it can be concluded that the doses of Co60 gamma radiation used in this study were shown to have a negative effect on the morphological and physiological characteristics evaluated in this work. Regarding the activity of antioxidant enzymes (SOD and APX), the shoots of seedlings of castor bean was more sensitive to gamma radiation Co60 used. |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-03-27 2014-08-20T13:59:15Z |
dc.date.issued.fl_str_mv |
2012-03-01 |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:59:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
LOPES, Amanda Moreira. Morphophysiological and biochemical changes in castor bean seeds (Ricinus communis L.) subjected to gamma Radiation Cobalt-60.. 2012. 58 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2057 |
identifier_str_mv |
LOPES, Amanda Moreira. Morphophysiological and biochemical changes in castor bean seeds (Ricinus communis L.) subjected to gamma Radiation Cobalt-60.. 2012. 58 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2057 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Fisiologia Vegetal |
dc.publisher.initials.fl_str_mv |
UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Biologia |
publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
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