Avaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2476 |
Resumo: | The treatment of gastrointestinal disorders using natural methods such as electroacupuncture and phytotherapy, has been deployed worldwide in human and veterinary medicine. Thus, the work was to determine the action of electroacupuncture and the aqueous and ethanol extracts of Rosmarinus officinalis on gastric motility of rats assessed by radiographic examination, in addition to reporting a case of Situs Inversus Totalis in a Wistar albino rat, diagnosed during the experiment. For evaluation of electroacupuncture on gastric motility, we used 24 Wistar rats, divided into four treatments, T1: electroacupuncture point E36 and BP6; T2: electroacupuncture in falses stitches; T3: sterile distilled water and T4: metoclopramide. Five metallic spheres were administered per animal and performed serial radiographs to monitor the gastric emptying time in the different treatments. By analyzing the time of exit the first spheres, the animals of group T1 ha d an average of 3,5h; in T2 the average was greater than 6h; T3 was 5,3h and the T4 observed average of 4,6h. the action of electroacupuncture was comparable to the action of metoclopramide, which is know to increase peristalsis, whereas the response of the Shan group (T2) was close to the negative control group. In the determination of gastric motility with the use of aqueous and ethanol extracts of R. officinalis, were used 48 albino rats, divided into eight groups, group TE1: ethanolic extract 125mg/kg; group TE2: ethanolic extract 250mg/kg; group TE3: ethanolic extract 500mg/kg; group TA1: aqueous extract 125mg/kg; group TA2: aqueous extract 250mg/kg; group TA3: aqueous extract 125mg/kg; group T4: metoclopramide, and group T5: sterile distilled water. After the treatments were administered five metallic spheres per animal and performed serial radiographs, each hour, to monitor the gastric emptying time. By analyzing the amount of spheres that left the stomach of experimental animals up to 3h, it was observed that there was no difference in treatment, as to the number of spheres removed. Were eliminated 76,6% in TE1, in TE2 60% (n=18), in group TE3 63,3% (n=19), in TA1 (n=14) 46,6%, in group TA2 50% (n=15) and, finally, the group TA3 86,6% (n=26) while in the control groups (T4) was 100% (n=30) and T5 93,3% (n=28). During the study, was diagnosed by radiographic examination, a rare case of Situs Inversus Totalis in albino rat, showing all the organs of the opposite side to its physiological position. Thus, it is concluded that electroacupuncture at points BP6 and E36, showed a significant increase of gastric peristalsis, reducing emptying time. In addition, the aqueous and ethanolic extracts of R. officinalis decrease gastric motility in rats. Suggesting that these may be useful in disorders of motility, however further studies should be conducted in order to better determine this action. The report of Situs Inversus Totalis draws attention to the importance of this type of diagnosis in order to remove the animal from the reproduction, as there are not sufficient studies to determine the reflection of the health condition of animals. |
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2014-08-20T14:37:50Z2012-08-292014-08-20T14:37:50Z2012-02-27CARNEVALI, Taiane Rita. Radiological evaluation of gastric motility of Wistar rats, subjected to the action of electroacupuncture and extract of Rosmarinus officinalis. 2012. 81 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2012.http://repositorio.ufpel.edu.br/handle/ri/2476The treatment of gastrointestinal disorders using natural methods such as electroacupuncture and phytotherapy, has been deployed worldwide in human and veterinary medicine. Thus, the work was to determine the action of electroacupuncture and the aqueous and ethanol extracts of Rosmarinus officinalis on gastric motility of rats assessed by radiographic examination, in addition to reporting a case of Situs Inversus Totalis in a Wistar albino rat, diagnosed during the experiment. For evaluation of electroacupuncture on gastric motility, we used 24 Wistar rats, divided into four treatments, T1: electroacupuncture point E36 and BP6; T2: electroacupuncture in falses stitches; T3: sterile distilled water and T4: metoclopramide. Five metallic spheres were administered per animal and performed serial radiographs to monitor the gastric emptying time in the different treatments. By analyzing the time of exit the first spheres, the animals of group T1 ha d an average of 3,5h; in T2 the average was greater than 6h; T3 was 5,3h and the T4 observed average of 4,6h. the action of electroacupuncture was comparable to the action of metoclopramide, which is know to increase peristalsis, whereas the response of the Shan group (T2) was close to the negative control group. In the determination of gastric motility with the use of aqueous and ethanol extracts of R. officinalis, were used 48 albino rats, divided into eight groups, group TE1: ethanolic extract 125mg/kg; group TE2: ethanolic extract 250mg/kg; group TE3: ethanolic extract 500mg/kg; group TA1: aqueous extract 125mg/kg; group TA2: aqueous extract 250mg/kg; group TA3: aqueous extract 125mg/kg; group T4: metoclopramide, and group T5: sterile distilled water. After the treatments were administered five metallic spheres per animal and performed serial radiographs, each hour, to monitor the gastric emptying time. By analyzing the amount of spheres that left the stomach of experimental animals up to 3h, it was observed that there was no difference in treatment, as to the number of spheres removed. Were eliminated 76,6% in TE1, in TE2 60% (n=18), in group TE3 63,3% (n=19), in TA1 (n=14) 46,6%, in group TA2 50% (n=15) and, finally, the group TA3 86,6% (n=26) while in the control groups (T4) was 100% (n=30) and T5 93,3% (n=28). During the study, was diagnosed by radiographic examination, a rare case of Situs Inversus Totalis in albino rat, showing all the organs of the opposite side to its physiological position. Thus, it is concluded that electroacupuncture at points BP6 and E36, showed a significant increase of gastric peristalsis, reducing emptying time. In addition, the aqueous and ethanolic extracts of R. officinalis decrease gastric motility in rats. Suggesting that these may be useful in disorders of motility, however further studies should be conducted in order to better determine this action. The report of Situs Inversus Totalis draws attention to the importance of this type of diagnosis in order to remove the animal from the reproduction, as there are not sufficient studies to determine the reflection of the health condition of animals.O tratamento das afecções gastrointestinais com o uso de métodos naturais, como a eletroacupuntura e a fitoterapia, vem se destacado mundialmente na medicina humana e animal. Assim, o trabalho teve como objetivos determinar a ação da eletroacupuntura e dos extratos aquoso e etanólico de Rosmarinus officinalis na motilidade gástrica de ratos wistar avaliados através de exame radiográfico, além de relatar um caso de Situs Inversus Totalis em um rato albino wistar, diagnosticado durante o experimento. Para avaliação da eletroacupuntura na motilidade gástrica, foram utilizados 24 ratos wistar, divididos em quatro tratamentos, sendo T1: eletroacupuntura nos pontos BP6 e E36; T2: eletroacupuntura em pontos falsos; T3: água destilada estéril e T4: etoclopramida. Foram administradas cinco esferas metálicas por animal e realizada radiografias seriadas a fim de acompanhar o tempo de esvaziamento gástrico nos diferentes tratamentos. Ao analisar o momento de saída das primeiras esferas, os animais do grupo T1 apresentaram uma média de 3,5h; no T2 a média foi maior que 6h, no T3 foi de 5,3h e no T4 observou-se média de 4,6h. A ação da eletroacupuntura foi comparável à ação da metoclopramida, que sabidamente aumenta o peristaltismo. Enquanto que a resposta do grupo Shan (T2) aproximou-se do grupo controle negativo. Na determinação da motilidade gástrica com uso de extratos aquoso e etanólico de R. officinalis, foram utilizados 48 ratos albinos, divididos em oito grupos, sendo grupo TE1: Extrato Etanólico 125mg/kg; grupo TE2: Extrato Etanólico 250mg/kg; grupo TE3: Extrato Etanólico 500mg/kg; grupo TA1: Extrato Aquoso 125mg/kg; grupo TA2: Extrato Aquoso 250mg/kg; grupo TA3: Extrato Aquoso 500mg/kg; grupo T4: Metoclopramida e grupo T5: Água destilada estéril. Após os tratamentos foram administradas cinco esferas metálicas por animal e realizada radiografias seriadas, de hora em hora, a fim de acompanhar o tempo de esvaziamento gástrico. Ao analisar a quantidade de esferas que saíram do estômago dos animais experimentais em até 3h, observou-se que houve diferença nos tratamentos, quanto ao numero de esferas eliminadas. Foram eliminadas 76,6% no TE1 (n=23), no TE2 60% (n=18), no grupo TE3 63,3% (n=19), no TA1 (n=14) 46,6%, no grupo TA2 50% (n=15) e, por fim, no grupo TA3 86,6% (n=26) enquanto nos grupos controle, (T4) foi de 100% (n=30) e T5 93,3% (n=28). Durante o estudo, diagnosticou-se através de exame radiográfico, um caso de Situs Inversus Totalis em rato albino, demonstrando todos órgãos do lado contrário a sua posição fisiológica. Com isso, conclui-se que a eletroacupuntura nos pontos BP6 e E36, apresentaram aumento significativo do peristaltismo gástrico, diminuindo assim o tempo de esvaziamento. Além disso, os extratos aquoso e etanólico de R. officinalis diminuem a motilidade gástrica em ratos wistar. Sugerindo que estes podem ser uteis nos distúrbios de motilidade, entretanto novos estudos devem ser conduzidos a fim de determinar melhor esta ação. O relato de Situs Inversus Totalis chama a atenção para a importância deste tipo de diagnóstico a fim de retirar o animal da reprodução, já que não existem estudos suficientes para determinar o reflexo da afecção na sanidade dos animais.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em VeterináriaUFPelBRVeterináriaRaio-XGastrointestinalRatosAlecrimAcupunturaÓrgãosTopografiaX-rayGastrointestinalRatsRosemaryAcupunctureOrgansTopographyCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAAvaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalisRadiological evaluation of gastric motility of Wistar rats, subjected to the action of electroacupuncture and extract of Rosmarinus officinalisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/6093845397805133http://lattes.cnpq.br/9916986411712495Cleff, Marlete BrumCarnevali, Taiane Ritainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_taiane_rita_carnevali.pdfapplication/pdf887366http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2476/1/dissertacao_taiane_rita_carnevali.pdf632a655ec8902ce8c4250491e710c930MD51open accessTEXTdissertacao_taiane_rita_carnevali.pdf.txtdissertacao_taiane_rita_carnevali.pdf.txtExtracted Texttext/plain120308http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2476/2/dissertacao_taiane_rita_carnevali.pdf.txtaa5830ee534968933e838cdaf5210271MD52open accessTHUMBNAILdissertacao_taiane_rita_carnevali.pdf.jpgdissertacao_taiane_rita_carnevali.pdf.jpgGenerated Thumbnailimage/jpeg1347http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2476/3/dissertacao_taiane_rita_carnevali.pdf.jpgf8d9b2b64b97252e93a1670704e240d4MD53open access123456789/24762019-09-17 19:12:23.013open accessoai:guaiaca.ufpel.edu.br:123456789/2476Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-17T22:12:23Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Avaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalis |
dc.title.alternative.eng.fl_str_mv |
Radiological evaluation of gastric motility of Wistar rats, subjected to the action of electroacupuncture and extract of Rosmarinus officinalis |
title |
Avaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalis |
spellingShingle |
Avaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalis Carnevali, Taiane Rita Raio-X Gastrointestinal Ratos Alecrim Acupuntura Órgãos Topografia X-ray Gastrointestinal Rats Rosemary Acupuncture Organs Topography CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Avaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalis |
title_full |
Avaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalis |
title_fullStr |
Avaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalis |
title_full_unstemmed |
Avaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalis |
title_sort |
Avaliação radiológica da motilidade gástrica de ratos wistar, submetidos a ação da eletroacupuntura e de extratos de Rosmarinus officinalis |
author |
Carnevali, Taiane Rita |
author_facet |
Carnevali, Taiane Rita |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/6093845397805133 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/9916986411712495 |
dc.contributor.advisor1.fl_str_mv |
Cleff, Marlete Brum |
dc.contributor.author.fl_str_mv |
Carnevali, Taiane Rita |
contributor_str_mv |
Cleff, Marlete Brum |
dc.subject.por.fl_str_mv |
Raio-X Gastrointestinal Ratos Alecrim Acupuntura Órgãos Topografia |
topic |
Raio-X Gastrointestinal Ratos Alecrim Acupuntura Órgãos Topografia X-ray Gastrointestinal Rats Rosemary Acupuncture Organs Topography CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
X-ray Gastrointestinal Rats Rosemary Acupuncture Organs Topography |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The treatment of gastrointestinal disorders using natural methods such as electroacupuncture and phytotherapy, has been deployed worldwide in human and veterinary medicine. Thus, the work was to determine the action of electroacupuncture and the aqueous and ethanol extracts of Rosmarinus officinalis on gastric motility of rats assessed by radiographic examination, in addition to reporting a case of Situs Inversus Totalis in a Wistar albino rat, diagnosed during the experiment. For evaluation of electroacupuncture on gastric motility, we used 24 Wistar rats, divided into four treatments, T1: electroacupuncture point E36 and BP6; T2: electroacupuncture in falses stitches; T3: sterile distilled water and T4: metoclopramide. Five metallic spheres were administered per animal and performed serial radiographs to monitor the gastric emptying time in the different treatments. By analyzing the time of exit the first spheres, the animals of group T1 ha d an average of 3,5h; in T2 the average was greater than 6h; T3 was 5,3h and the T4 observed average of 4,6h. the action of electroacupuncture was comparable to the action of metoclopramide, which is know to increase peristalsis, whereas the response of the Shan group (T2) was close to the negative control group. In the determination of gastric motility with the use of aqueous and ethanol extracts of R. officinalis, were used 48 albino rats, divided into eight groups, group TE1: ethanolic extract 125mg/kg; group TE2: ethanolic extract 250mg/kg; group TE3: ethanolic extract 500mg/kg; group TA1: aqueous extract 125mg/kg; group TA2: aqueous extract 250mg/kg; group TA3: aqueous extract 125mg/kg; group T4: metoclopramide, and group T5: sterile distilled water. After the treatments were administered five metallic spheres per animal and performed serial radiographs, each hour, to monitor the gastric emptying time. By analyzing the amount of spheres that left the stomach of experimental animals up to 3h, it was observed that there was no difference in treatment, as to the number of spheres removed. Were eliminated 76,6% in TE1, in TE2 60% (n=18), in group TE3 63,3% (n=19), in TA1 (n=14) 46,6%, in group TA2 50% (n=15) and, finally, the group TA3 86,6% (n=26) while in the control groups (T4) was 100% (n=30) and T5 93,3% (n=28). During the study, was diagnosed by radiographic examination, a rare case of Situs Inversus Totalis in albino rat, showing all the organs of the opposite side to its physiological position. Thus, it is concluded that electroacupuncture at points BP6 and E36, showed a significant increase of gastric peristalsis, reducing emptying time. In addition, the aqueous and ethanolic extracts of R. officinalis decrease gastric motility in rats. Suggesting that these may be useful in disorders of motility, however further studies should be conducted in order to better determine this action. The report of Situs Inversus Totalis draws attention to the importance of this type of diagnosis in order to remove the animal from the reproduction, as there are not sufficient studies to determine the reflection of the health condition of animals. |
publishDate |
2012 |
dc.date.available.fl_str_mv |
2012-08-29 2014-08-20T14:37:50Z |
dc.date.issued.fl_str_mv |
2012-02-27 |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:37:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
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masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CARNEVALI, Taiane Rita. Radiological evaluation of gastric motility of Wistar rats, subjected to the action of electroacupuncture and extract of Rosmarinus officinalis. 2012. 81 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2476 |
identifier_str_mv |
CARNEVALI, Taiane Rita. Radiological evaluation of gastric motility of Wistar rats, subjected to the action of electroacupuncture and extract of Rosmarinus officinalis. 2012. 81 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2012. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2476 |
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por |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Veterinária |
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UFPel |
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BR |
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Veterinária |
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Universidade Federal de Pelotas |
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