Eficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermática
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2564 |
Resumo: | The objectives of this study were to determine the main bacteria present in ram semen and to evaluate the efficiency of distinct antibiotics in controlling such microorganisms and on sperm viability. Ejaculates were collected from five rams and extended in TRIS-egg yolk. In Experiment 1, the following treatments were tested: control, with no antibiotics; GTLS, with 500 μg/ml of gentamicin, 100 μg/ml of tylosin, 300 μg/ml of lincomycin and 600 μg/ml of spectinomycin; PENSTREP, with 500 μg/ml of penicillin and 100 μg/ml of streptomycin; CEFT, with 50 μg/ml of sodium ceftiofur; and ENRO, with 1000 μg/ml of enrofloxacin. For bacteria identification, samples were collected: the preputial ostium; the artificial vagina; the ejaculates; the semen pool from the five rams; after treatments allocation and stabilization at 5°C; and after thawing. In Experiment 2, variation in the antibiotics concentrations used in Experiment 1 were tested: 25% higher (GTLS+25, PENSTREP+25, CEFT+25 and ENRO+25); 50% higher (GTLS+50, PENSTREP+50, CEFT+50 and ENRO+50); 25% lower (GTLS-25, PENSTREP-25, CEFT-25 and ENRO-25); and 50% lower (GTLS-50, PENSTREP-50, CEFT-50 and ENRO-50). Samples of ram semen were used for counting colony forming units (CFU). The isolated microorganisms were Staphylococcus sp., Klebsiella sp., Corynebacterium sp. and Bacillus sp. The ENRO treatment presented the lowest sperm motility for semen cooled at 5°C and after thawing (P < 0.05), although the post-thawing motility did not differ from that observed for the CEFT and GTLS treatments (P > 0.05). No differences were observed among treatments regarding sperm membrane, acrosome and sperm DNA integrity (P > 0.05). The post-thawing proportion of sperm having DNA integrity was greater for the PENSTREP than for the GTLS treatment (P < 0.05), although sperm DNA integrity may have been impaired by the freezing-thawing process. In experiment 2, the GTLS+50, PENSTREP, ENRO+25 and ENRO+50 treatments presented reduced sperm motility (P < 0.05). All treatments reduced the number of CFU in comparison with the control treatment (P < 0.05). The CFU for the GTLS+50, ENRO+25 and ENRO+50 treatments and the concentrations tested for both those treatments did not differ (P < 0.05), although both were more effective than the PENSTREP and CEFT treatments (P < 0.05). However, the ENRO treatment was associated with reduced sperm motility, especially with high antibiotic concentrations. The treatments did not influence sperm morphology and the integrity of sperm membrane, acrosome and DNA. |
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2014-08-20T14:38:01Z2011-06-012014-08-20T14:38:01Z2011-02-28MADEIRA, Elisângela Mirapalheta. Efficiency of antibiotics included in extenders for cryopreservation of ram semen and their influence on sperm viability. 2011. 46 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2011.http://repositorio.ufpel.edu.br/handle/ri/2564The objectives of this study were to determine the main bacteria present in ram semen and to evaluate the efficiency of distinct antibiotics in controlling such microorganisms and on sperm viability. Ejaculates were collected from five rams and extended in TRIS-egg yolk. In Experiment 1, the following treatments were tested: control, with no antibiotics; GTLS, with 500 μg/ml of gentamicin, 100 μg/ml of tylosin, 300 μg/ml of lincomycin and 600 μg/ml of spectinomycin; PENSTREP, with 500 μg/ml of penicillin and 100 μg/ml of streptomycin; CEFT, with 50 μg/ml of sodium ceftiofur; and ENRO, with 1000 μg/ml of enrofloxacin. For bacteria identification, samples were collected: the preputial ostium; the artificial vagina; the ejaculates; the semen pool from the five rams; after treatments allocation and stabilization at 5°C; and after thawing. In Experiment 2, variation in the antibiotics concentrations used in Experiment 1 were tested: 25% higher (GTLS+25, PENSTREP+25, CEFT+25 and ENRO+25); 50% higher (GTLS+50, PENSTREP+50, CEFT+50 and ENRO+50); 25% lower (GTLS-25, PENSTREP-25, CEFT-25 and ENRO-25); and 50% lower (GTLS-50, PENSTREP-50, CEFT-50 and ENRO-50). Samples of ram semen were used for counting colony forming units (CFU). The isolated microorganisms were Staphylococcus sp., Klebsiella sp., Corynebacterium sp. and Bacillus sp. The ENRO treatment presented the lowest sperm motility for semen cooled at 5°C and after thawing (P < 0.05), although the post-thawing motility did not differ from that observed for the CEFT and GTLS treatments (P > 0.05). No differences were observed among treatments regarding sperm membrane, acrosome and sperm DNA integrity (P > 0.05). The post-thawing proportion of sperm having DNA integrity was greater for the PENSTREP than for the GTLS treatment (P < 0.05), although sperm DNA integrity may have been impaired by the freezing-thawing process. In experiment 2, the GTLS+50, PENSTREP, ENRO+25 and ENRO+50 treatments presented reduced sperm motility (P < 0.05). All treatments reduced the number of CFU in comparison with the control treatment (P < 0.05). The CFU for the GTLS+50, ENRO+25 and ENRO+50 treatments and the concentrations tested for both those treatments did not differ (P < 0.05), although both were more effective than the PENSTREP and CEFT treatments (P < 0.05). However, the ENRO treatment was associated with reduced sperm motility, especially with high antibiotic concentrations. The treatments did not influence sperm morphology and the integrity of sperm membrane, acrosome and DNA.Este estudo tem como objetivos determinar os principais gêneros bacterianos presentes no sêmen ovino e avaliar a ação de diferentes antibióticos no controle destes microrganismos e o seu impacto sobre a viabilidade espermática. Todos os ejaculados foram coletados de cinco machos ovinos e diluídos em Tris-gema de ovo. No experimento 1 foram testados os seguintes tratamentos: Controle sem antibiótico; GTLS, com 500 μg/ml de gentamicina, 100 μg/ml de tilosina, 300 μg/ml de lincomicina e 600 μg/ml de espectinomicina; PENSTREP, com 500 μg/ml de penicilina e 100 μg/ml de estreptomicina; CEFT, com 50 μg/ml de ceftiofur sódico; e ENRO, com 1000 μg/ml de enrofloxacina. Para a identificação dos gêneros bacterianos presentes, amostras foram colhidas do óstio prepucial, da vagina artificial, dos ejaculados carneiros, do pool de sêmen dos cinco carneiros e após a inclusão dos tratamentos e estabilização a 5 °C e após o descongelamento. No experimento 2, foram testadas variações nas concentrações de antibióticos avaliadas no Experimento 1: 25% acima (GTLS+25; PENSTREP+25; CEFT+25; ENRO+25), 50% acima (GTLS+50; PENSTREP+50; CEFT+50; ENRO+50), 25% abaixo (GTLS-25; PENSTREP-25; CEFT-25; ENRO-25) e 50% abaixo (GTLS-50; PENSTREP-50; CEFT-50; ENRO-50). Amostras de sêmen dos carneiros foram utilizadas para contagem das unidades formadoras de colônias (UFC). Os microrganismos isolados foram Staphylococcus sp., Klebsiella sp., Corynebacterium sp. e Bacillus sp. O tratamento com enrofloxacina apresentou a motilidade espermática mais baixa para o sêmen refrigerado a 5 °C e após o descongelamento (P<0,05), no entanto a motilidade pósdescongelamento não diferiu (P>0,05) dos tratamentos CEFT e GTLS. Não foram observadas diferenças entre os tratamentos quanto à integridade da membrana plasmática, do acrossoma e do DNA espermático (P>0,05). Após o descongelamento, o percentual de espermatozóides com DNA íntegro foi maior (P<0,05) no tratamento PENSTREP em relação ao GTLS, apesar de que este parâmetro pode ter sido afetado pelo processo de criopreservação. No experimento 2, GTLS+50, PENSTREP, ENRO+25 e ENRO+50 foram os tratamentos que apresentaram redução na motilidade espermática (P<0,05). Todos os tratamentos reduziram consideravelmente o número de UFC em relação ao grupo controle. A contagem de UFC para os tratamentos GTLS+50, ENRO+25 e ENRO+50 e as variações de concentração para ambos não foram diferentes entre si (P>0,05), porém ambos foram mais efetivos que os tratamentos PENSTREP e CEFT (P<0,05). Porém, o tratamento com enrofloxacina prejudicou a motilidade espermática, especialmente quando se elevou a concentração Os tratamentos não influenciaram a morfologia, integridade da membrana plasmática, integridade de acrossoma e integridade de DNA dos espermatozóides.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em VeterináriaUFPelBRVeterináriaAntibióticosViabilidade espermáticaBactériasDiluenteSêmen ovinoAntibioticsSperm viabilityBacteriaExtenderRam semenCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAEficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermáticaEfficiency of antibiotics included in extenders for cryopreservation of ram semen and their influence on sperm viabilityinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/0440763526903975http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768000E7Leite, Fabio Pereira Leivashttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763076J5Bianchi, IvanMadeira, Elisângela Mirapalhetainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_elisangela_madeira.pdfapplication/pdf269361http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2564/1/dissertacao_elisangela_madeira.pdf0f80449efc17473198ff8837f06f02f4MD51open accessTEXTdissertacao_elisangela_madeira.pdf.txtdissertacao_elisangela_madeira.pdf.txtExtracted Texttext/plain66456http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2564/2/dissertacao_elisangela_madeira.pdf.txte7a991aa9fe0cf8d06ff5139c18bdd47MD52open accessTHUMBNAILdissertacao_elisangela_madeira.pdf.jpgdissertacao_elisangela_madeira.pdf.jpgGenerated Thumbnailimage/jpeg1825http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2564/3/dissertacao_elisangela_madeira.pdf.jpg67bee6bb83f12eaffacb995227cc0033MD53open access123456789/25642019-09-25 11:18:34.292open accessoai:guaiaca.ufpel.edu.br:123456789/2564Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-25T14:18:34Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Eficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermática |
dc.title.alternative.eng.fl_str_mv |
Efficiency of antibiotics included in extenders for cryopreservation of ram semen and their influence on sperm viability |
title |
Eficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermática |
spellingShingle |
Eficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermática Madeira, Elisângela Mirapalheta Antibióticos Viabilidade espermática Bactérias Diluente Sêmen ovino Antibiotics Sperm viability Bacteria Extender Ram semen CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Eficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermática |
title_full |
Eficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermática |
title_fullStr |
Eficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermática |
title_full_unstemmed |
Eficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermática |
title_sort |
Eficácia da inclusão de antibióticos em diluente para criopreservação de sêmen ovino e sua influência na viabilidade espermática |
author |
Madeira, Elisângela Mirapalheta |
author_facet |
Madeira, Elisângela Mirapalheta |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/0440763526903975 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4768000E7 |
dc.contributor.advisor-co1.fl_str_mv |
Leite, Fabio Pereira Leivas |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763076J5 |
dc.contributor.advisor1.fl_str_mv |
Bianchi, Ivan |
dc.contributor.author.fl_str_mv |
Madeira, Elisângela Mirapalheta |
contributor_str_mv |
Leite, Fabio Pereira Leivas Bianchi, Ivan |
dc.subject.por.fl_str_mv |
Antibióticos Viabilidade espermática Bactérias Diluente Sêmen ovino |
topic |
Antibióticos Viabilidade espermática Bactérias Diluente Sêmen ovino Antibiotics Sperm viability Bacteria Extender Ram semen CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Antibiotics Sperm viability Bacteria Extender Ram semen |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
The objectives of this study were to determine the main bacteria present in ram semen and to evaluate the efficiency of distinct antibiotics in controlling such microorganisms and on sperm viability. Ejaculates were collected from five rams and extended in TRIS-egg yolk. In Experiment 1, the following treatments were tested: control, with no antibiotics; GTLS, with 500 μg/ml of gentamicin, 100 μg/ml of tylosin, 300 μg/ml of lincomycin and 600 μg/ml of spectinomycin; PENSTREP, with 500 μg/ml of penicillin and 100 μg/ml of streptomycin; CEFT, with 50 μg/ml of sodium ceftiofur; and ENRO, with 1000 μg/ml of enrofloxacin. For bacteria identification, samples were collected: the preputial ostium; the artificial vagina; the ejaculates; the semen pool from the five rams; after treatments allocation and stabilization at 5°C; and after thawing. In Experiment 2, variation in the antibiotics concentrations used in Experiment 1 were tested: 25% higher (GTLS+25, PENSTREP+25, CEFT+25 and ENRO+25); 50% higher (GTLS+50, PENSTREP+50, CEFT+50 and ENRO+50); 25% lower (GTLS-25, PENSTREP-25, CEFT-25 and ENRO-25); and 50% lower (GTLS-50, PENSTREP-50, CEFT-50 and ENRO-50). Samples of ram semen were used for counting colony forming units (CFU). The isolated microorganisms were Staphylococcus sp., Klebsiella sp., Corynebacterium sp. and Bacillus sp. The ENRO treatment presented the lowest sperm motility for semen cooled at 5°C and after thawing (P < 0.05), although the post-thawing motility did not differ from that observed for the CEFT and GTLS treatments (P > 0.05). No differences were observed among treatments regarding sperm membrane, acrosome and sperm DNA integrity (P > 0.05). The post-thawing proportion of sperm having DNA integrity was greater for the PENSTREP than for the GTLS treatment (P < 0.05), although sperm DNA integrity may have been impaired by the freezing-thawing process. In experiment 2, the GTLS+50, PENSTREP, ENRO+25 and ENRO+50 treatments presented reduced sperm motility (P < 0.05). All treatments reduced the number of CFU in comparison with the control treatment (P < 0.05). The CFU for the GTLS+50, ENRO+25 and ENRO+50 treatments and the concentrations tested for both those treatments did not differ (P < 0.05), although both were more effective than the PENSTREP and CEFT treatments (P < 0.05). However, the ENRO treatment was associated with reduced sperm motility, especially with high antibiotic concentrations. The treatments did not influence sperm morphology and the integrity of sperm membrane, acrosome and DNA. |
publishDate |
2011 |
dc.date.available.fl_str_mv |
2011-06-01 2014-08-20T14:38:01Z |
dc.date.issued.fl_str_mv |
2011-02-28 |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:38:01Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
MADEIRA, Elisângela Mirapalheta. Efficiency of antibiotics included in extenders for cryopreservation of ram semen and their influence on sperm viability. 2011. 46 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2564 |
identifier_str_mv |
MADEIRA, Elisângela Mirapalheta. Efficiency of antibiotics included in extenders for cryopreservation of ram semen and their influence on sperm viability. 2011. 46 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2011. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2564 |
dc.language.iso.fl_str_mv |
por |
language |
por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Veterinária |
dc.publisher.initials.fl_str_mv |
UFPel |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Veterinária |
publisher.none.fl_str_mv |
Universidade Federal de Pelotas |
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reponame:Repositório Institucional da UFPel - Guaiaca instname:Universidade Federal de Pelotas (UFPEL) instacron:UFPEL |
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Universidade Federal de Pelotas (UFPEL) |
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UFPEL |
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UFPEL |
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