Estabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do Sul
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2024 |
Resumo: | Maytenus ilicifolia Mart. ex Reis., popularly known as espinheira-santa, is an autochthonous specie belonging to Celastraceae family. Presents high medicinal value for treatment of ulcers, chronicle gastritis, dyspepsia and indigestion. Due its medicinal importance, have been an increase of extractivism in natural populations. So, this specie is priority for conservation, to avoid the risk of genetic erosion. The tissue culture is important because allows massive production of plants free from pathogenic agents and genetically uniforms. The in vitro establishment is the first phase of the micro propagation process, where the plant material used can vary according to the finality of the work. Studies evidence that genetic flux by seeds or pollen grains, and also the environment, mainly the habitats, has the potential to improve the genetic variability of populations from one locality. The objective of this work was to establish and evaluate the capacity of in vitro multiplication of M. ilicifolia plantlet, and also analyze by AFLP the genetic variability of 20 accessions collected in different localities from Rio Grande do Sul State. For the in vitro establishment previously disinfected seeds were inoculated in three different media: MS; MS + Active Coal (1g L-1) and MS + Vermiculite. The variables analyzed were plantlet height and root length. For the molecular analysis it was tested eight primers combinations. Values of genetic similarities were calculated by Simple-matching coefficient and used to generate a similarity dendrograma by UPGMA method. The analyzed variables showed significant differences. The greater media for plantlet height (5.06 cm) and root x length (3.4 cm) were obtained in the MS medium with Vermiculite. The morphological aspect of the roots must be considered, because the roots from plantlets germinated in MS medium was very fibrous and without root hairs, differently from plantlets germinated the MS medium + Vermiculite, were presented roots adequate for absorption of nutrients from the medium culture. The eight primers generate 455 eletrophoretical profiles, obtaining 100% of polymorphism. The primers E-ACC/M-CAA, E-ACG/M-CTA and E-ACG/M-CTC presented the greater number of eletrophoretical profile, 71 each, totalizing 46.80% of the total polymorphism. Dendrograma analysis showed a high coefficient of correlation (r = 0.94), demonstrating high representativity from the data of genetic similarity and those of groupings. By AMOVA it was verified that 89.33% of the total variability happened among individuals from the same population. Based on the discussed can be concluded that the more adequate medium for the in vitro establishment is the MS medium added of Vermiculite and AFLP molecular markers allow the characterization and genetic variability estimative of M. ilicifolia populations. |
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2014-08-20T13:59:09Z2008-09-292014-08-20T13:59:09Z2008-07-08RIBEIRO, Márcia Vaz. In vitro establishment and analysis of genetic variability of Maytenus ilicifolia accessions collected at Rio Grande do Sul State, Brazil. 2008. 55 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2008.http://repositorio.ufpel.edu.br/handle/ri/2024Maytenus ilicifolia Mart. ex Reis., popularly known as espinheira-santa, is an autochthonous specie belonging to Celastraceae family. Presents high medicinal value for treatment of ulcers, chronicle gastritis, dyspepsia and indigestion. Due its medicinal importance, have been an increase of extractivism in natural populations. So, this specie is priority for conservation, to avoid the risk of genetic erosion. The tissue culture is important because allows massive production of plants free from pathogenic agents and genetically uniforms. The in vitro establishment is the first phase of the micro propagation process, where the plant material used can vary according to the finality of the work. Studies evidence that genetic flux by seeds or pollen grains, and also the environment, mainly the habitats, has the potential to improve the genetic variability of populations from one locality. The objective of this work was to establish and evaluate the capacity of in vitro multiplication of M. ilicifolia plantlet, and also analyze by AFLP the genetic variability of 20 accessions collected in different localities from Rio Grande do Sul State. For the in vitro establishment previously disinfected seeds were inoculated in three different media: MS; MS + Active Coal (1g L-1) and MS + Vermiculite. The variables analyzed were plantlet height and root length. For the molecular analysis it was tested eight primers combinations. Values of genetic similarities were calculated by Simple-matching coefficient and used to generate a similarity dendrograma by UPGMA method. The analyzed variables showed significant differences. The greater media for plantlet height (5.06 cm) and root x length (3.4 cm) were obtained in the MS medium with Vermiculite. The morphological aspect of the roots must be considered, because the roots from plantlets germinated in MS medium was very fibrous and without root hairs, differently from plantlets germinated the MS medium + Vermiculite, were presented roots adequate for absorption of nutrients from the medium culture. The eight primers generate 455 eletrophoretical profiles, obtaining 100% of polymorphism. The primers E-ACC/M-CAA, E-ACG/M-CTA and E-ACG/M-CTC presented the greater number of eletrophoretical profile, 71 each, totalizing 46.80% of the total polymorphism. Dendrograma analysis showed a high coefficient of correlation (r = 0.94), demonstrating high representativity from the data of genetic similarity and those of groupings. By AMOVA it was verified that 89.33% of the total variability happened among individuals from the same population. Based on the discussed can be concluded that the more adequate medium for the in vitro establishment is the MS medium added of Vermiculite and AFLP molecular markers allow the characterization and genetic variability estimative of M. ilicifolia populations.Maytenus ilicifolia Mart. ex Reis., popularmente conhecida como espinheira-santa, é uma espécie autóctone pertencente à família Celastraceae que apresenta alto valor medicinal para o tratamento de úlceras, gastrites crônicas, dispepsias e indigestão. Devido a sua importância medicinal, houve um aumento no extrativismo em populações naturais, tornando-a prioritária para a conservação, a fim de evitar o risco de erosão genética. A cultura de tecidos tem importante papel por proporcionar produção massiva de plantas, livres de agentes patogênicos e geneticamente uniformes, sendo o estabelecimento in vitro a primeira fase do processo de micropropagação, onde o material vegetal utilizado pode variar de acordo com a finalidade do estudo. Estudos evidenciam que o fluxo gênico por sementes ou dispersão de grãos de pólen, bem como o ambiente, principalmente o habitat, tem o potencial de aumentar a variabilidade genética de populações de uma mesma localidade. O objetivo deste trabalho foi estabelecer e avaliar a capacidade de multiplicação in vitro de plântulas de M. ilicifolia, bem como, analisar a variabilidade genética de 20 acessos coletados em diferentes localidades no estado do Rio Grande do Sul, através da técnica AFLP. Para o estabelecimento in vitro, sementes previamente desinfestadas foram inoculadas em três meios diferentes: MS; MS + Carvão Ativado (1g L-1) e MS + Vermiculita. As variáveis analisadas foram altura das plântulas e comprimento de raiz. Para a viii análise molecular foram testadas oito combinações de primers AFLP. Os valores de similaridade genética foram calculados pelo Simple-matching coefficient e utilizados para gerar o dendrograma de similaridade pelo método UPGMA. As variáveis analisadas apresentaram diferença significativa, onde as maiores médias para altura das plântulas (5,06 cm) e comprimento de raiz (3,4 cm), foram obtidas no meio MS acrescido de Vermiculita. Observou-se que o aspecto morfológico das raízes deve ser considerado, pois as raízes das plântulas germinadas em meio MS apresentaram-se bastante fibrosas e com ausência de pêlos radiculares, diferentemente das plântulas germinadas em meio MS + Vermiculita, onde apresentaram raízes adequadas para a absorção de nutrientes do meio de cultura. As oito combinações de primers geraram 455 perfis eletroforéticos, com 100% de polimorfismo. Os primers E-ACC/M-CAA, E-ACG/MCTA, E-ACG/M-CTC apresentaram o maior número de perfis eletroforéticos, 71 cada, totalizando 46,80% do polimorfismo total. Na análise do dendrograma foi observado um alto coeficiente de correlação (r = 0,94), demonstrando elevada representatividade dos dados de similaridade genética e os de agrupamento. Pela AMOVA verificou-se que 89,33% da variabilidade total ocorreu entre indivíduos da mesma população. Com base no exposto conclui-se que o meio mais adequado para o estabelecimento in vitro é o meio MS acrescido de Vermiculita e marcadores moleculares tipo AFLP permitem a caracterização e a estimativa da variabilidade genética de populações de M. ilicifolia.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em Fisiologia VegetalUFPelBRBiologiaEspinheira-santaMaytenus ilicifoliaPlantas medicinaisCultivo in vitroSimilaridade genéticaAFLPPolimorfismoCNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETALEstabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do SulIn vitro establishment and analysis of genetic variability of Maytenus ilicifolia accessions collected at Rio Grande do Sul State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/7106367057929107http://lattes.cnpq.br/7705049074927773Barbieri, Rosa Liahttp://lattes.cnpq.br/4561611881845259Braga, Eugenia Jacira BolacelRibeiro, Márcia Vazinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_marcia_vaz_ribeiro.pdfapplication/pdf3498598http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2024/1/dissertacao_marcia_vaz_ribeiro.pdf7e2417006373e96bad648e6cfe508c8aMD51open accessTEXTdissertacao_marcia_vaz_ribeiro.pdf.txtdissertacao_marcia_vaz_ribeiro.pdf.txtExtracted Texttext/plain77100http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2024/2/dissertacao_marcia_vaz_ribeiro.pdf.txt3e8053b5a9b593401caeaca6776b3463MD52open accessTHUMBNAILdissertacao_marcia_vaz_ribeiro.pdf.jpgdissertacao_marcia_vaz_ribeiro.pdf.jpgGenerated Thumbnailimage/jpeg1372http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2024/3/dissertacao_marcia_vaz_ribeiro.pdf.jpg86c10f9a54782ee08b872a73ae6b0b0cMD53open access123456789/20242019-09-19 09:14:41.341open accessoai:guaiaca.ufpel.edu.br:123456789/2024Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-19T12:14:41Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Estabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do Sul |
dc.title.alternative.eng.fl_str_mv |
In vitro establishment and analysis of genetic variability of Maytenus ilicifolia accessions collected at Rio Grande do Sul State, Brazil |
title |
Estabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do Sul |
spellingShingle |
Estabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do Sul Ribeiro, Márcia Vaz Espinheira-santa Maytenus ilicifolia Plantas medicinais Cultivo in vitro Similaridade genética AFLP Polimorfismo CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
title_short |
Estabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do Sul |
title_full |
Estabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do Sul |
title_fullStr |
Estabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do Sul |
title_full_unstemmed |
Estabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do Sul |
title_sort |
Estabelecimento in vitro e análise da variabilidade genética de acessos de espinheira-santa (Maytenus ilicifolia) coletados no Rio Grande do Sul |
author |
Ribeiro, Márcia Vaz |
author_facet |
Ribeiro, Márcia Vaz |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/7106367057929107 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/7705049074927773 |
dc.contributor.referees1.pt_BR.fl_str_mv |
Barbieri, Rosa Lia |
dc.contributor.referees1ID.por.fl_str_mv |
|
dc.contributor.referees1Lattes.por.fl_str_mv |
http://lattes.cnpq.br/4561611881845259 |
dc.contributor.advisor1.fl_str_mv |
Braga, Eugenia Jacira Bolacel |
dc.contributor.author.fl_str_mv |
Ribeiro, Márcia Vaz |
contributor_str_mv |
Braga, Eugenia Jacira Bolacel |
dc.subject.por.fl_str_mv |
Espinheira-santa Maytenus ilicifolia Plantas medicinais Cultivo in vitro Similaridade genética AFLP Polimorfismo |
topic |
Espinheira-santa Maytenus ilicifolia Plantas medicinais Cultivo in vitro Similaridade genética AFLP Polimorfismo CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::BOTANICA::FISIOLOGIA VEGETAL |
description |
Maytenus ilicifolia Mart. ex Reis., popularly known as espinheira-santa, is an autochthonous specie belonging to Celastraceae family. Presents high medicinal value for treatment of ulcers, chronicle gastritis, dyspepsia and indigestion. Due its medicinal importance, have been an increase of extractivism in natural populations. So, this specie is priority for conservation, to avoid the risk of genetic erosion. The tissue culture is important because allows massive production of plants free from pathogenic agents and genetically uniforms. The in vitro establishment is the first phase of the micro propagation process, where the plant material used can vary according to the finality of the work. Studies evidence that genetic flux by seeds or pollen grains, and also the environment, mainly the habitats, has the potential to improve the genetic variability of populations from one locality. The objective of this work was to establish and evaluate the capacity of in vitro multiplication of M. ilicifolia plantlet, and also analyze by AFLP the genetic variability of 20 accessions collected in different localities from Rio Grande do Sul State. For the in vitro establishment previously disinfected seeds were inoculated in three different media: MS; MS + Active Coal (1g L-1) and MS + Vermiculite. The variables analyzed were plantlet height and root length. For the molecular analysis it was tested eight primers combinations. Values of genetic similarities were calculated by Simple-matching coefficient and used to generate a similarity dendrograma by UPGMA method. The analyzed variables showed significant differences. The greater media for plantlet height (5.06 cm) and root x length (3.4 cm) were obtained in the MS medium with Vermiculite. The morphological aspect of the roots must be considered, because the roots from plantlets germinated in MS medium was very fibrous and without root hairs, differently from plantlets germinated the MS medium + Vermiculite, were presented roots adequate for absorption of nutrients from the medium culture. The eight primers generate 455 eletrophoretical profiles, obtaining 100% of polymorphism. The primers E-ACC/M-CAA, E-ACG/M-CTA and E-ACG/M-CTC presented the greater number of eletrophoretical profile, 71 each, totalizing 46.80% of the total polymorphism. Dendrograma analysis showed a high coefficient of correlation (r = 0.94), demonstrating high representativity from the data of genetic similarity and those of groupings. By AMOVA it was verified that 89.33% of the total variability happened among individuals from the same population. Based on the discussed can be concluded that the more adequate medium for the in vitro establishment is the MS medium added of Vermiculite and AFLP molecular markers allow the characterization and genetic variability estimative of M. ilicifolia populations. |
publishDate |
2008 |
dc.date.available.fl_str_mv |
2008-09-29 2014-08-20T13:59:09Z |
dc.date.issued.fl_str_mv |
2008-07-08 |
dc.date.accessioned.fl_str_mv |
2014-08-20T13:59:09Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
RIBEIRO, Márcia Vaz. In vitro establishment and analysis of genetic variability of Maytenus ilicifolia accessions collected at Rio Grande do Sul State, Brazil. 2008. 55 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2008. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2024 |
identifier_str_mv |
RIBEIRO, Márcia Vaz. In vitro establishment and analysis of genetic variability of Maytenus ilicifolia accessions collected at Rio Grande do Sul State, Brazil. 2008. 55 f. Dissertação (Mestrado em Biologia) - Universidade Federal de Pelotas, Pelotas, 2008. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2024 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Fisiologia Vegetal |
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UFPel |
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BR |
dc.publisher.department.fl_str_mv |
Biologia |
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Universidade Federal de Pelotas |
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