Ocorrência de Campylobacter em carne de frango, fezes de frango e humanas e pesquisa dos genes cdt

• Autor(a) principal: Silva, Daiani Teixeira da
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Repositório Institucional da UFPel - Guaiaca
• Texto Completo: http://repositorio.ufpel.edu.br/handle/ri/2479
• topic: Campylobacter; Genes cdt; Fezes de frango; Fezes de humanos; Produtos de frango; Campylobacter; CDT genes; Chicken fezes; Human fezes; Poultry meat; CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA

Campylobacter is a bacteria related to foodborne illnesses. It is one of the most common causes of diarrhea in humans in the world. It might cause Guillain-Barre syndrome, which attacks the cells of the peripheral nervous system causing muscle paralysis. Some strains of this bacterium can produce a cytolethal distending toxin (CDT), which can exacerbate diarrheal diseases. Birds are the main reservoir of this micro-organism, of which they are asymptomatic hosts. During the slaughter of broilers, contamination of meat and its products may occur. They are the main responsible for infections in humans. The objective of this study was to verify the presence of C. jejuni and C. coli in poultry meat, chicken and human feces and investigate the presence of cdtA, cdtB and cdtC genes in the isolates. A hundred chicken fecal samples, a hundred samples of retail poultry products and a hundred samples of human feces were collected. The poultry products were washed with Brucella broth and spread onto plates of Columbia agar. The feces samples were directly spread onto plates containing this medium. Confirmation of isolates and species identification were performed by PCR. PCR was also used to investigate the presence of cdt genes. Campylobacter was found in 61% of the chicken fecal samples, in 20% of the poultry products and in 3% of the human feces. C. jejuni was the predominant species and 93,5% of them exhibited the cdt genes. Despite the fact that the prevalence of Campylobacter found in human feces was low, the occurrence of Campylobacter in broilers and poultry products sold in southern Rio Grande do Sul was high. Besides, it has been observed that most of the isolates were highly likely to produce CDT. These results suggest there is a need for preventive measures in the production system and good manufacturing practices in the industry so as to minimize contamination of products and reduce the risk to consumers.