Investigação in vitro da atividade antifúngica de novos compostos derivados de Pirazois
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2236 |
Resumo: | Various treatments have been suggested for the treatment of denture stomatitis but problems such as toxicity and antibiotic resistance can be observed. This study evaluated the in vitro antifungal activity, anti-enzymatic and cytotoxic new compounds pirazolínicos. Were used strains of Candida albicans (33), C. parapsilosis (2), C famata (2), C glabrata (2) and C lipolytica (2). The antifungal activity was evaluated by minimum inhibitory concentration (MIC) and Minimum Fungicide (MFC). The anti-enzyme activity was determined in agar medium proteinase and phospholipase. To test for cytotoxicity were used fibroblasts (3T3/NIH) and evaluated by colorimetric assays. The data were submitted to ANOVA and Tukey. The results were: MIC and MFC> 15.6 Sgml for C. albicans; MIC and MFC> 62.5 Sgml for C. parapsilosis, MIC and MFC = 62.5 Sgml for C. famata, MIC and MFC = 125 Sgml for C. glabrata and MIC = 15.6 Sgml for C. lipolytica. The average values of phospholipase and proteinase (Pz) of C. albicans before and after exposure were: 0.6 (± 0.024) and 0.2 (± 0.022) and 0.9 (± 0.074) and 0.3 (± 0.04). These results were not statistically significant for proteinase (p = 0.69) but significant for phospholipase (p = 0.01), and the concentration of 15.6 Sgml the most effective. There were no statistical differences between the groups and the control on the cytotoxicity (p = 0.32). It was found that the derivatives are promising pirazolínicos antifungal agents, either by killing the yeasts or inhibition of the enzyme activity, and its low cytotoxicity |
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2014-08-20T14:30:08Z2014-01-242014-08-20T14:30:08Z2012-05-21OLIVEIRA, Simone Gomes Dias de. Research in vitro antifungal activity of new compounds derived from pyrazoles. 2012. 77 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2012.http://repositorio.ufpel.edu.br/handle/ri/2236Various treatments have been suggested for the treatment of denture stomatitis but problems such as toxicity and antibiotic resistance can be observed. This study evaluated the in vitro antifungal activity, anti-enzymatic and cytotoxic new compounds pirazolínicos. Were used strains of Candida albicans (33), C. parapsilosis (2), C famata (2), C glabrata (2) and C lipolytica (2). The antifungal activity was evaluated by minimum inhibitory concentration (MIC) and Minimum Fungicide (MFC). The anti-enzyme activity was determined in agar medium proteinase and phospholipase. To test for cytotoxicity were used fibroblasts (3T3/NIH) and evaluated by colorimetric assays. The data were submitted to ANOVA and Tukey. The results were: MIC and MFC> 15.6 Sgml for C. albicans; MIC and MFC> 62.5 Sgml for C. parapsilosis, MIC and MFC = 62.5 Sgml for C. famata, MIC and MFC = 125 Sgml for C. glabrata and MIC = 15.6 Sgml for C. lipolytica. The average values of phospholipase and proteinase (Pz) of C. albicans before and after exposure were: 0.6 (± 0.024) and 0.2 (± 0.022) and 0.9 (± 0.074) and 0.3 (± 0.04). These results were not statistically significant for proteinase (p = 0.69) but significant for phospholipase (p = 0.01), and the concentration of 15.6 Sgml the most effective. There were no statistical differences between the groups and the control on the cytotoxicity (p = 0.32). It was found that the derivatives are promising pirazolínicos antifungal agents, either by killing the yeasts or inhibition of the enzyme activity, and its low cytotoxicityDiversos medicamentos antifungicos vêm sendo sugeridos para o tratamento da estomatite protética porém problemas como toxidade e resistência antimicrobiana podem ser observados. Este estudo objetivou avaliar in vitro o potencial antifúngico, anti-enzimático e citotóxico de novos compostos pirazolínicos. Utilizou-se cepas de Candida albicans(33), C. parapsilosis(2), C. famata(2), C. glabrata(2), C. lipolytica(2). A atividade antifúngica foi avaliada pela Concentração Inibitória Mínima (CIM) e Fungicida Mínima (CFM). A atividade anti-enzimática foi determinada em meios ágar proteinase e fosfolipase. Para o teste de citotoxidade, foram utilizados fibroblastos (3T3/NIH) e avaliado através de ensaio colorimétrico. Os dados foram submetidos aos testes ANOVA e Correlação de Spearman. Os resultados foram: CIM e CFM>15,6Sgml para C. albicans; CIM e CFM>62,5 Sgml para C. parapsilosis; CIM e CFM=62,5 Sgml para C. famata; CIM e CFM=125 Sgml para C. glabrata e CIM=15,6 Sgml para C. lipolytica. Os valores médios de fosfolipase e proteinase (Pz) de C. albicans antes e após a exposição foram respectivamente: 0,2 (±0,022) e 0,6 (±0,024) ; 0,3(±0,04) e 0,9(±0,074) . Estes resultados não foram estatisticamente significantes para proteinase, porém significantes para fosfolipase (p=0,01), sendo a concentração de 15,6 Sgml a mais efetiva. Não foram observadas diferenças estatísticas entre os grupos testados e o controle quanto à citotoxidade. Concluiu-se que os derivados pirazolínicos são promissores agentes antifúngicos, seja através da morte das leveduras ou inibição da sua atividade enzimática, além de apresentarem baixa citotoxidadeapplication/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em OdontologiaUFPelBROdontologiaTestes de sensibilidade microbianaCandida albicansEstomatite sob próteseCandidaDenture stomatitisAntifungal agentsCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAInvestigação in vitro da atividade antifúngica de novos compostos derivados de PirazoisResearch in vitro antifungal activity of new compounds derived from pyrazolesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/8536579510075779http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701753T0Lund, Rafael Guerrahttp://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776008U0Pereira, Claudio Martin Pereira dehttp://lattes.cnpq.br/5722353488752184Piva, EvandroOliveira, Simone Gomes Dias deinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALDissertacao_simone_gomes_dias_oliveira.pdfapplication/pdf1514143http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2236/1/Dissertacao_simone_gomes_dias_oliveira.pdfc839385a830bc5abebda0ffb2d706d95MD51open accessTEXTDissertacao_simone_gomes_dias_oliveira.pdf.txtDissertacao_simone_gomes_dias_oliveira.pdf.txtExtracted Texttext/plain115815http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2236/2/Dissertacao_simone_gomes_dias_oliveira.pdf.txt8cf7a5848387072d1d652a12ad4ce0b5MD52open accessTHUMBNAILDissertacao_simone_gomes_dias_oliveira.pdf.jpgDissertacao_simone_gomes_dias_oliveira.pdf.jpgGenerated Thumbnailimage/jpeg1988http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2236/3/Dissertacao_simone_gomes_dias_oliveira.pdf.jpg377bb402398e5e8832df7df9d03c0485MD53open access123456789/22362019-09-25 11:28:27.754open accessoai:guaiaca.ufpel.edu.br:123456789/2236Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-25T14:28:27Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Investigação in vitro da atividade antifúngica de novos compostos derivados de Pirazois |
dc.title.alternative.eng.fl_str_mv |
Research in vitro antifungal activity of new compounds derived from pyrazoles |
title |
Investigação in vitro da atividade antifúngica de novos compostos derivados de Pirazois |
spellingShingle |
Investigação in vitro da atividade antifúngica de novos compostos derivados de Pirazois Oliveira, Simone Gomes Dias de Testes de sensibilidade microbiana Candida albicans Estomatite sob prótese Candida Denture stomatitis Antifungal agents CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
title_short |
Investigação in vitro da atividade antifúngica de novos compostos derivados de Pirazois |
title_full |
Investigação in vitro da atividade antifúngica de novos compostos derivados de Pirazois |
title_fullStr |
Investigação in vitro da atividade antifúngica de novos compostos derivados de Pirazois |
title_full_unstemmed |
Investigação in vitro da atividade antifúngica de novos compostos derivados de Pirazois |
title_sort |
Investigação in vitro da atividade antifúngica de novos compostos derivados de Pirazois |
author |
Oliveira, Simone Gomes Dias de |
author_facet |
Oliveira, Simone Gomes Dias de |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/8536579510075779 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4701753T0 |
dc.contributor.advisor-co1.fl_str_mv |
Lund, Rafael Guerra |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4776008U0 |
dc.contributor.advisor-co2.fl_str_mv |
Pereira, Claudio Martin Pereira de |
dc.contributor.advisor-co2Lattes.fl_str_mv |
http://lattes.cnpq.br/5722353488752184 |
dc.contributor.advisor1.fl_str_mv |
Piva, Evandro |
dc.contributor.author.fl_str_mv |
Oliveira, Simone Gomes Dias de |
contributor_str_mv |
Lund, Rafael Guerra Pereira, Claudio Martin Pereira de Piva, Evandro |
dc.subject.por.fl_str_mv |
Testes de sensibilidade microbiana Candida albicans Estomatite sob prótese |
topic |
Testes de sensibilidade microbiana Candida albicans Estomatite sob prótese Candida Denture stomatitis Antifungal agents CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
dc.subject.eng.fl_str_mv |
Candida Denture stomatitis Antifungal agents |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA |
description |
Various treatments have been suggested for the treatment of denture stomatitis but problems such as toxicity and antibiotic resistance can be observed. This study evaluated the in vitro antifungal activity, anti-enzymatic and cytotoxic new compounds pirazolínicos. Were used strains of Candida albicans (33), C. parapsilosis (2), C famata (2), C glabrata (2) and C lipolytica (2). The antifungal activity was evaluated by minimum inhibitory concentration (MIC) and Minimum Fungicide (MFC). The anti-enzyme activity was determined in agar medium proteinase and phospholipase. To test for cytotoxicity were used fibroblasts (3T3/NIH) and evaluated by colorimetric assays. The data were submitted to ANOVA and Tukey. The results were: MIC and MFC> 15.6 Sgml for C. albicans; MIC and MFC> 62.5 Sgml for C. parapsilosis, MIC and MFC = 62.5 Sgml for C. famata, MIC and MFC = 125 Sgml for C. glabrata and MIC = 15.6 Sgml for C. lipolytica. The average values of phospholipase and proteinase (Pz) of C. albicans before and after exposure were: 0.6 (± 0.024) and 0.2 (± 0.022) and 0.9 (± 0.074) and 0.3 (± 0.04). These results were not statistically significant for proteinase (p = 0.69) but significant for phospholipase (p = 0.01), and the concentration of 15.6 Sgml the most effective. There were no statistical differences between the groups and the control on the cytotoxicity (p = 0.32). It was found that the derivatives are promising pirazolínicos antifungal agents, either by killing the yeasts or inhibition of the enzyme activity, and its low cytotoxicity |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-05-21 |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:30:08Z |
dc.date.available.fl_str_mv |
2014-01-24 2014-08-20T14:30:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
OLIVEIRA, Simone Gomes Dias de. Research in vitro antifungal activity of new compounds derived from pyrazoles. 2012. 77 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2236 |
identifier_str_mv |
OLIVEIRA, Simone Gomes Dias de. Research in vitro antifungal activity of new compounds derived from pyrazoles. 2012. 77 f. Dissertação (Mestrado em Odontologia) - Universidade Federal de Pelotas, Pelotas, 2012. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2236 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Odontologia |
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UFPel |
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BR |
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Odontologia |
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Universidade Federal de Pelotas |
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