Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFPel - Guaiaca |
Texto Completo: | http://repositorio.ufpel.edu.br/handle/ri/2567 |
Resumo: | Currently, the drug industry looks for new drugs based on natural products, for the production of drugs more efficient, for which the microorganisms did not show resistance to both humans and animals. A natural product that has been the subject of intense pharmacological and chemical studies by scientists for the control of diseases is propolis, a resinous substance produced by honeybees from exudates collected from different parts of the plant, which has been used for centuries in popular medicine due to its therapeutic properties. Chemical studies revealed the complex chemical composition, identifying in some cases more than 300 components including various bioactive phenolic compounds responsible for the virucidal action. This work initially describes the standardization of an ethanol extract of green propolis (EEPV), where the chemicals were identified by high performance liquid chromatography (HPLC), phytochemical characterization by thin layer chromatography (TLC), soluble solids, content of phenolics and flavonoids and antioxidant activity by 2.2 diphenil picryl hydrazyl (DPPH). The EEPV was also evaluated in vitro and in vivo for their capacity lentogenic virucidal against a strain of the virus of Newcastle disease (NDV) at two different temperatures (22 and 37 ° C), 5 incubation periods (0, 1, 2, 4 and 8 hours) of NDV in five different concentrations of EEPV (4000μg/dose, 400μg/dose, 40μg/dose, and 4μg/dose 0μg/dose). The EEPV standard is within the standards required by the MAP, with high levels of phenolics and flavonoids (12.93 and 6.05% respectively) as shown by HPLC, which identified high concentrations of phenolic acids (p-coumaric acid, hydroxycinnamic acid diprenyl , cinâmino acid derivatives), which are assigned the antibacterial, antioxidant, antiviral and virucidal. This extract showed dose-dependent virucidal activity (4000μg/dose e 400μg/dose) and time of incubation with the virus (2 hour). The inhibitory activity of EEPV against the strain of NDV lentogenic found in the present study suggests the use of this extract as an alternative to fight the infection by this virus. |
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2014-08-20T14:38:03Z2011-06-022014-08-20T14:38:03Z2011-02-28NUNES, Cristina Freitas. Virucidal activity of ethanol extract of green propolis in vitro. 2011. 85 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2011.http://repositorio.ufpel.edu.br/handle/ri/2567Currently, the drug industry looks for new drugs based on natural products, for the production of drugs more efficient, for which the microorganisms did not show resistance to both humans and animals. A natural product that has been the subject of intense pharmacological and chemical studies by scientists for the control of diseases is propolis, a resinous substance produced by honeybees from exudates collected from different parts of the plant, which has been used for centuries in popular medicine due to its therapeutic properties. Chemical studies revealed the complex chemical composition, identifying in some cases more than 300 components including various bioactive phenolic compounds responsible for the virucidal action. This work initially describes the standardization of an ethanol extract of green propolis (EEPV), where the chemicals were identified by high performance liquid chromatography (HPLC), phytochemical characterization by thin layer chromatography (TLC), soluble solids, content of phenolics and flavonoids and antioxidant activity by 2.2 diphenil picryl hydrazyl (DPPH). The EEPV was also evaluated in vitro and in vivo for their capacity lentogenic virucidal against a strain of the virus of Newcastle disease (NDV) at two different temperatures (22 and 37 ° C), 5 incubation periods (0, 1, 2, 4 and 8 hours) of NDV in five different concentrations of EEPV (4000μg/dose, 400μg/dose, 40μg/dose, and 4μg/dose 0μg/dose). The EEPV standard is within the standards required by the MAP, with high levels of phenolics and flavonoids (12.93 and 6.05% respectively) as shown by HPLC, which identified high concentrations of phenolic acids (p-coumaric acid, hydroxycinnamic acid diprenyl , cinâmino acid derivatives), which are assigned the antibacterial, antioxidant, antiviral and virucidal. This extract showed dose-dependent virucidal activity (4000μg/dose e 400μg/dose) and time of incubation with the virus (2 hour). The inhibitory activity of EEPV against the strain of NDV lentogenic found in the present study suggests the use of this extract as an alternative to fight the infection by this virus.Atualmente, a indústria farmacêutica busca novos medicamentos com base em produtos naturais, visando à produção de fármacos mais eficientes, para os quais os microrganismos não apresentem resistência, tanto para humanos quanto para animais. Um dos produtos naturais que tem sido objeto de intensos estudos farmacológicos e químicos por cientistas para o controle de enfermidades é a própolis, uma substância resinosa produzida por abelhas melíferas a partir de exsudatos coletados em diferentes partes das plantas, que tem sido utilizada durante séculos na medicina popular devido as suas propriedades terapêuticas. Estudos químicos revelaram a complexa composição da própolis, identificando em alguns casos mais de 300 componentes, incluindo vários compostos bioativos fenólicos responsáveis pela ação virucida. Este trabalho inicialmente descreve a padronização de um extrato etanólico de própolis verde (EEPV), onde foram identificados os compostos químicos por cromatografia liquida de alta eficiência (CLAE), caracterização fitoquímica por cromatografia em camada delgada (CCD), teor de sólidos solúveis, teor de fenóis e flavonóides totais e atividade antioxidante por 2,2 diphenil picril hidrazil(DPPH). O EEPV foi avaliado também in vitro e in vivo, quanto a sua capacidade virucida contra uma cepa lentogênica do vírus da doença de Newcastle (NDV) em duas temperaturas distintas (22 e 37°C), 5 períodos de incubação (0, 1, 2, 4 e 8 horas) do NDV em 5 concentrações de EEPV distintos (4000μg/dose, 400μg/dose, 40μg/dose, 4μg/dose e 0μg/dose). O EEPV padronizado está dentro dos padrões requisitados pelo MAPA, com altos níveis de fenóis e flavonóides totais (12.93 e 6,05% respectivamente), comprovado por CLAE, o qual identificou altas concentrações de ácidos fenólicos (ácido p-cumárico, ácido diprenil hidroxicinâmico, derivados do ácido cinâmino), os quais são atribuídos as propriedades antibacteriana, antioxidante, antiviral e virucida. Este extrato apresentou atividade virucida dependente da dose (4000μg/dose, 400μg/dose) e do tempo de incubação com o vírus (2 horas). A atividade inibitória do EEPV contra a cepa lentogênica de NDV, encontrada no presente estudo sugere a utilização deste extrato como uma alternativa no combate a infecções por este vírus.application/pdfporUniversidade Federal de PelotasPrograma de Pós-Graduação em VeterináriaUFPelBRVeterináriaPrópolis verdeFenóis totaisVirucidaNDVParamyxovirusStandardizationGreen propolisTotals phenolicsVirucidalNDVParamyxovirusCNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIAAtividade virucida de um extrato etanólico de própolis verde in vitro e in vivoVirucidal activity of ethanol extract of green propolis in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesishttp://lattes.cnpq.br/2585277612564392http://lattes.cnpq.br/2905761117990157Fischer, Gefersonhttp://lattes.cnpq.br/6163020760854309Vargas, Gilberto D'ÁvilaNunes, Cristina Freitasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFPel - Guaiacainstname:Universidade Federal de Pelotas (UFPEL)instacron:UFPELORIGINALdissertacao_cristina_nunes.pdfapplication/pdf1093744http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2567/1/dissertacao_cristina_nunes.pdf0c42a534dae5175850fd939832f086a8MD51open accessTEXTdissertacao_cristina_nunes.pdf.txtdissertacao_cristina_nunes.pdf.txtExtracted Texttext/plain147296http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2567/2/dissertacao_cristina_nunes.pdf.txtb5674030c49bf0068a722590a7ba83acMD52open accessTHUMBNAILdissertacao_cristina_nunes.pdf.jpgdissertacao_cristina_nunes.pdf.jpgGenerated Thumbnailimage/jpeg1724http://guaiaca.ufpel.edu.br/xmlui/bitstream/123456789/2567/3/dissertacao_cristina_nunes.pdf.jpgd44c919adcc4d65960f8a1f9281a10eaMD53open access123456789/25672019-09-13 09:51:34.709open accessoai:guaiaca.ufpel.edu.br:123456789/2567Repositório InstitucionalPUBhttp://repositorio.ufpel.edu.br/oai/requestrippel@ufpel.edu.br || repositorio@ufpel.edu.br || aline.batista@ufpel.edu.bropendoar:2019-09-13T12:51:34Repositório Institucional da UFPel - Guaiaca - Universidade Federal de Pelotas (UFPEL)false |
dc.title.por.fl_str_mv |
Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo |
dc.title.alternative.eng.fl_str_mv |
Virucidal activity of ethanol extract of green propolis in vitro |
title |
Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo |
spellingShingle |
Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo Nunes, Cristina Freitas Própolis verde Fenóis totais Virucida NDV Paramyxovirus Standardization Green propolis Totals phenolics Virucidal NDV Paramyxovirus CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
title_short |
Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo |
title_full |
Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo |
title_fullStr |
Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo |
title_full_unstemmed |
Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo |
title_sort |
Atividade virucida de um extrato etanólico de própolis verde in vitro e in vivo |
author |
Nunes, Cristina Freitas |
author_facet |
Nunes, Cristina Freitas |
author_role |
author |
dc.contributor.authorLattes.por.fl_str_mv |
http://lattes.cnpq.br/2585277612564392 |
dc.contributor.advisorLattes.por.fl_str_mv |
http://lattes.cnpq.br/2905761117990157 |
dc.contributor.advisor-co1.fl_str_mv |
Fischer, Geferson |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/6163020760854309 |
dc.contributor.advisor1.fl_str_mv |
Vargas, Gilberto D'Ávila |
dc.contributor.author.fl_str_mv |
Nunes, Cristina Freitas |
contributor_str_mv |
Fischer, Geferson Vargas, Gilberto D'Ávila |
dc.subject.por.fl_str_mv |
Própolis verde Fenóis totais Virucida NDV Paramyxovirus |
topic |
Própolis verde Fenóis totais Virucida NDV Paramyxovirus Standardization Green propolis Totals phenolics Virucidal NDV Paramyxovirus CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
dc.subject.eng.fl_str_mv |
Standardization Green propolis Totals phenolics Virucidal NDV Paramyxovirus |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS AGRARIAS::MEDICINA VETERINARIA |
description |
Currently, the drug industry looks for new drugs based on natural products, for the production of drugs more efficient, for which the microorganisms did not show resistance to both humans and animals. A natural product that has been the subject of intense pharmacological and chemical studies by scientists for the control of diseases is propolis, a resinous substance produced by honeybees from exudates collected from different parts of the plant, which has been used for centuries in popular medicine due to its therapeutic properties. Chemical studies revealed the complex chemical composition, identifying in some cases more than 300 components including various bioactive phenolic compounds responsible for the virucidal action. This work initially describes the standardization of an ethanol extract of green propolis (EEPV), where the chemicals were identified by high performance liquid chromatography (HPLC), phytochemical characterization by thin layer chromatography (TLC), soluble solids, content of phenolics and flavonoids and antioxidant activity by 2.2 diphenil picryl hydrazyl (DPPH). The EEPV was also evaluated in vitro and in vivo for their capacity lentogenic virucidal against a strain of the virus of Newcastle disease (NDV) at two different temperatures (22 and 37 ° C), 5 incubation periods (0, 1, 2, 4 and 8 hours) of NDV in five different concentrations of EEPV (4000μg/dose, 400μg/dose, 40μg/dose, and 4μg/dose 0μg/dose). The EEPV standard is within the standards required by the MAP, with high levels of phenolics and flavonoids (12.93 and 6.05% respectively) as shown by HPLC, which identified high concentrations of phenolic acids (p-coumaric acid, hydroxycinnamic acid diprenyl , cinâmino acid derivatives), which are assigned the antibacterial, antioxidant, antiviral and virucidal. This extract showed dose-dependent virucidal activity (4000μg/dose e 400μg/dose) and time of incubation with the virus (2 hour). The inhibitory activity of EEPV against the strain of NDV lentogenic found in the present study suggests the use of this extract as an alternative to fight the infection by this virus. |
publishDate |
2011 |
dc.date.available.fl_str_mv |
2011-06-02 2014-08-20T14:38:03Z |
dc.date.issued.fl_str_mv |
2011-02-28 |
dc.date.accessioned.fl_str_mv |
2014-08-20T14:38:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NUNES, Cristina Freitas. Virucidal activity of ethanol extract of green propolis in vitro. 2011. 85 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2011. |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufpel.edu.br/handle/ri/2567 |
identifier_str_mv |
NUNES, Cristina Freitas. Virucidal activity of ethanol extract of green propolis in vitro. 2011. 85 f. Dissertação (Mestrado em Veterinária) - Universidade Federal de Pelotas, Pelotas, 2011. |
url |
http://repositorio.ufpel.edu.br/handle/ri/2567 |
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por |
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por |
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Universidade Federal de Pelotas |
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Programa de Pós-Graduação em Veterinária |
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UFPel |
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BR |
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Veterinária |
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Universidade Federal de Pelotas |
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