Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions

Detalhes bibliográficos
Autor(a) principal: Lazarotto, Marília
Data de Publicação: 2014
Outros Autores: Mezzomo, Ricardo, Gonzatto Maciel, Caciara, Finger, Geísa, Brião Muniz, Marlove Fátima
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Ciências Agrárias (Belém. Online)
Texto Completo: https://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142
Resumo: The Fusarium genus exhibits morphological, physiological and genetic variation among its different species, which may lead to differences in the environmental conditions required for their cultivation. This study aimed to verify the effects of different culture media and lighting regimes on the mycelial growth and sporulation of Fusarium chlamydosporum species complex isolates that are pathogenic to Carya illinoinensis. Four isolates from infected Carya illinoinensis roots and inflorescences were selected; these isolates were purified and subsequently transferred to four different culture media: potato sucrose agar (PSA), water agar (WA), potato dextrose agar (PDA) and carnation leaf agar (CLA). The isolates were then submitted to three different lighting regimes: a photoperiod of 12 h (TL), continuous light (CL) and continuous darkness (CD). After seven days of incubation under the three lighting regimes, the mycelial growth was evaluated on PSA, WA and PDA, and sporulation was evaluated on PSA, WA, PDA and CLA. In general, PSA and BDA media support mycelial growth and sporulation, particularly under CL. CLA medium also supports sporulation, particularly under CL. The CD lighting regime and the WA medium are not recommended for either the mycelial growth or sporulation of the F. chlamydosporum species complex.
id UFRA_7beb01e9eb915338bc7cb893430f1b86
oai_identifier_str oai:ojs.www.periodicos.ufra.edu.br:article/1142
network_acronym_str UFRA
network_name_str Revista de Ciências Agrárias (Belém. Online)
repository_id_str
spelling Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditionsCrescimento micelial e esporulação de complexo de espécies chlamydosporum Fusarium sob diferentes condições de cultivoThe Fusarium genus exhibits morphological, physiological and genetic variation among its different species, which may lead to differences in the environmental conditions required for their cultivation. This study aimed to verify the effects of different culture media and lighting regimes on the mycelial growth and sporulation of Fusarium chlamydosporum species complex isolates that are pathogenic to Carya illinoinensis. Four isolates from infected Carya illinoinensis roots and inflorescences were selected; these isolates were purified and subsequently transferred to four different culture media: potato sucrose agar (PSA), water agar (WA), potato dextrose agar (PDA) and carnation leaf agar (CLA). The isolates were then submitted to three different lighting regimes: a photoperiod of 12 h (TL), continuous light (CL) and continuous darkness (CD). After seven days of incubation under the three lighting regimes, the mycelial growth was evaluated on PSA, WA and PDA, and sporulation was evaluated on PSA, WA, PDA and CLA. In general, PSA and BDA media support mycelial growth and sporulation, particularly under CL. CLA medium also supports sporulation, particularly under CL. The CD lighting regime and the WA medium are not recommended for either the mycelial growth or sporulation of the F. chlamydosporum species complex.  O gênero Fusarium apresenta variação morfológica , fisiológica e genética entre suas diferentes espécies, o que pode levar a diferenças nas condições ambientais necessárias para o seu cultivo . Este estudo teve como objetivo verificar os efeitos de diferentes meios de cultura e regimes de luz sobre o crescimento micelial e esporulação de espécies chlamydosporum Fusarium isolados complexos que são patogênicas para Carya illinoinensis . Quatro isolados de Carya infectado illinoinensis raízes e inflorescências foram selecionados , estes isolados foram purificados e posteriormente transferidos para quatro meios de cultura diferentes : ágar batata sacarose (PSA) , ágar água (WA) , ágar batata dextrose (PDA) e agar folha cravo (CLA ) . Os isolados foram então submetidos a três diferentes regimes de iluminação : fotoperíodo de 12 h (TL) , luz contínua (CL) e escuridão contínua (CD). Depois de sete dias de incubação, sob os três regimes de iluminação , o crescimento micelial foi avaliado em PSA , WA e PDA , e esporulação foi avaliada em PSA , WA , PDA e CLA . Em geral, PSA e BDA mídia apoiar o crescimento micelial e esporulação , particularmente sob CL. Médio CLA também suporta esporulação , particularmente sob CL. O regime de iluminação CD eo meio WA não são recomendados tanto para o crescimento micelial ou esporulação do complexo de espécies chlamydosporum F. .Universidade Federal Rural da Amazônia/UFRA2014-02-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 57 No 1 (2014): RCA; 35-40Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 57 n. 1 (2014): RCA; 35-402177-87601517-591Xreponame:Revista de Ciências Agrárias (Belém. Online)instname:Universidade Federal Rural da Amazônia (UFRA)instacron:UFRAporhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142/431Lazarotto, MaríliaMezzomo, RicardoGonzatto Maciel, CaciaraFinger, GeísaBrião Muniz, Marlove Fátimainfo:eu-repo/semantics/openAccess2017-07-18T18:41:52Zoai:ojs.www.periodicos.ufra.edu.br:article/1142Revistahttps://ajaes.ufra.edu.br/index.php/ajaes/PUBhttps://ajaes.ufra.edu.br/index.php/ajaes/oaiallan.lobato@ufra.edu.br || ajaes.suporte@gmail.com2177-87601517-591Xopendoar:2017-07-18T18:41:52Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA)false
dc.title.none.fl_str_mv Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions
Crescimento micelial e esporulação de complexo de espécies chlamydosporum Fusarium sob diferentes condições de cultivo
title Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions
spellingShingle Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions
Lazarotto, Marília
title_short Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions
title_full Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions
title_fullStr Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions
title_full_unstemmed Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions
title_sort Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions
author Lazarotto, Marília
author_facet Lazarotto, Marília
Mezzomo, Ricardo
Gonzatto Maciel, Caciara
Finger, Geísa
Brião Muniz, Marlove Fátima
author_role author
author2 Mezzomo, Ricardo
Gonzatto Maciel, Caciara
Finger, Geísa
Brião Muniz, Marlove Fátima
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lazarotto, Marília
Mezzomo, Ricardo
Gonzatto Maciel, Caciara
Finger, Geísa
Brião Muniz, Marlove Fátima
description The Fusarium genus exhibits morphological, physiological and genetic variation among its different species, which may lead to differences in the environmental conditions required for their cultivation. This study aimed to verify the effects of different culture media and lighting regimes on the mycelial growth and sporulation of Fusarium chlamydosporum species complex isolates that are pathogenic to Carya illinoinensis. Four isolates from infected Carya illinoinensis roots and inflorescences were selected; these isolates were purified and subsequently transferred to four different culture media: potato sucrose agar (PSA), water agar (WA), potato dextrose agar (PDA) and carnation leaf agar (CLA). The isolates were then submitted to three different lighting regimes: a photoperiod of 12 h (TL), continuous light (CL) and continuous darkness (CD). After seven days of incubation under the three lighting regimes, the mycelial growth was evaluated on PSA, WA and PDA, and sporulation was evaluated on PSA, WA, PDA and CLA. In general, PSA and BDA media support mycelial growth and sporulation, particularly under CL. CLA medium also supports sporulation, particularly under CL. The CD lighting regime and the WA medium are not recommended for either the mycelial growth or sporulation of the F. chlamydosporum species complex.
publishDate 2014
dc.date.none.fl_str_mv 2014-02-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142
url https://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142/431
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural da Amazônia/UFRA
publisher.none.fl_str_mv Universidade Federal Rural da Amazônia/UFRA
dc.source.none.fl_str_mv Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 57 No 1 (2014): RCA; 35-40
Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 57 n. 1 (2014): RCA; 35-40
2177-8760
1517-591X
reponame:Revista de Ciências Agrárias (Belém. Online)
instname:Universidade Federal Rural da Amazônia (UFRA)
instacron:UFRA
instname_str Universidade Federal Rural da Amazônia (UFRA)
instacron_str UFRA
institution UFRA
reponame_str Revista de Ciências Agrárias (Belém. Online)
collection Revista de Ciências Agrárias (Belém. Online)
repository.name.fl_str_mv Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA)
repository.mail.fl_str_mv allan.lobato@ufra.edu.br || ajaes.suporte@gmail.com
_version_ 1797231627536957440