Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Ciências Agrárias (Belém. Online) |
Texto Completo: | https://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142 |
Resumo: | The Fusarium genus exhibits morphological, physiological and genetic variation among its different species, which may lead to differences in the environmental conditions required for their cultivation. This study aimed to verify the effects of different culture media and lighting regimes on the mycelial growth and sporulation of Fusarium chlamydosporum species complex isolates that are pathogenic to Carya illinoinensis. Four isolates from infected Carya illinoinensis roots and inflorescences were selected; these isolates were purified and subsequently transferred to four different culture media: potato sucrose agar (PSA), water agar (WA), potato dextrose agar (PDA) and carnation leaf agar (CLA). The isolates were then submitted to three different lighting regimes: a photoperiod of 12 h (TL), continuous light (CL) and continuous darkness (CD). After seven days of incubation under the three lighting regimes, the mycelial growth was evaluated on PSA, WA and PDA, and sporulation was evaluated on PSA, WA, PDA and CLA. In general, PSA and BDA media support mycelial growth and sporulation, particularly under CL. CLA medium also supports sporulation, particularly under CL. The CD lighting regime and the WA medium are not recommended for either the mycelial growth or sporulation of the F. chlamydosporum species complex. |
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Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditionsCrescimento micelial e esporulação de complexo de espécies chlamydosporum Fusarium sob diferentes condições de cultivoThe Fusarium genus exhibits morphological, physiological and genetic variation among its different species, which may lead to differences in the environmental conditions required for their cultivation. This study aimed to verify the effects of different culture media and lighting regimes on the mycelial growth and sporulation of Fusarium chlamydosporum species complex isolates that are pathogenic to Carya illinoinensis. Four isolates from infected Carya illinoinensis roots and inflorescences were selected; these isolates were purified and subsequently transferred to four different culture media: potato sucrose agar (PSA), water agar (WA), potato dextrose agar (PDA) and carnation leaf agar (CLA). The isolates were then submitted to three different lighting regimes: a photoperiod of 12 h (TL), continuous light (CL) and continuous darkness (CD). After seven days of incubation under the three lighting regimes, the mycelial growth was evaluated on PSA, WA and PDA, and sporulation was evaluated on PSA, WA, PDA and CLA. In general, PSA and BDA media support mycelial growth and sporulation, particularly under CL. CLA medium also supports sporulation, particularly under CL. The CD lighting regime and the WA medium are not recommended for either the mycelial growth or sporulation of the F. chlamydosporum species complex. O gênero Fusarium apresenta variação morfológica , fisiológica e genética entre suas diferentes espécies, o que pode levar a diferenças nas condições ambientais necessárias para o seu cultivo . Este estudo teve como objetivo verificar os efeitos de diferentes meios de cultura e regimes de luz sobre o crescimento micelial e esporulação de espécies chlamydosporum Fusarium isolados complexos que são patogênicas para Carya illinoinensis . Quatro isolados de Carya infectado illinoinensis raízes e inflorescências foram selecionados , estes isolados foram purificados e posteriormente transferidos para quatro meios de cultura diferentes : ágar batata sacarose (PSA) , ágar água (WA) , ágar batata dextrose (PDA) e agar folha cravo (CLA ) . Os isolados foram então submetidos a três diferentes regimes de iluminação : fotoperíodo de 12 h (TL) , luz contínua (CL) e escuridão contínua (CD). Depois de sete dias de incubação, sob os três regimes de iluminação , o crescimento micelial foi avaliado em PSA , WA e PDA , e esporulação foi avaliada em PSA , WA , PDA e CLA . Em geral, PSA e BDA mídia apoiar o crescimento micelial e esporulação , particularmente sob CL. Médio CLA também suporta esporulação , particularmente sob CL. O regime de iluminação CD eo meio WA não são recomendados tanto para o crescimento micelial ou esporulação do complexo de espécies chlamydosporum F. .Universidade Federal Rural da Amazônia/UFRA2014-02-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 57 No 1 (2014): RCA; 35-40Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 57 n. 1 (2014): RCA; 35-402177-87601517-591Xreponame:Revista de Ciências Agrárias (Belém. Online)instname:Universidade Federal Rural da Amazônia (UFRA)instacron:UFRAporhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142/431Lazarotto, MaríliaMezzomo, RicardoGonzatto Maciel, CaciaraFinger, GeísaBrião Muniz, Marlove Fátimainfo:eu-repo/semantics/openAccess2017-07-18T18:41:52Zoai:ojs.www.periodicos.ufra.edu.br:article/1142Revistahttps://ajaes.ufra.edu.br/index.php/ajaes/PUBhttps://ajaes.ufra.edu.br/index.php/ajaes/oaiallan.lobato@ufra.edu.br || ajaes.suporte@gmail.com2177-87601517-591Xopendoar:2017-07-18T18:41:52Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA)false |
dc.title.none.fl_str_mv |
Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions Crescimento micelial e esporulação de complexo de espécies chlamydosporum Fusarium sob diferentes condições de cultivo |
title |
Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions |
spellingShingle |
Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions Lazarotto, Marília |
title_short |
Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions |
title_full |
Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions |
title_fullStr |
Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions |
title_full_unstemmed |
Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions |
title_sort |
Mycelia growth and sporulation of Fusarium chlamydosporum species complex under different culture conditions |
author |
Lazarotto, Marília |
author_facet |
Lazarotto, Marília Mezzomo, Ricardo Gonzatto Maciel, Caciara Finger, Geísa Brião Muniz, Marlove Fátima |
author_role |
author |
author2 |
Mezzomo, Ricardo Gonzatto Maciel, Caciara Finger, Geísa Brião Muniz, Marlove Fátima |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lazarotto, Marília Mezzomo, Ricardo Gonzatto Maciel, Caciara Finger, Geísa Brião Muniz, Marlove Fátima |
description |
The Fusarium genus exhibits morphological, physiological and genetic variation among its different species, which may lead to differences in the environmental conditions required for their cultivation. This study aimed to verify the effects of different culture media and lighting regimes on the mycelial growth and sporulation of Fusarium chlamydosporum species complex isolates that are pathogenic to Carya illinoinensis. Four isolates from infected Carya illinoinensis roots and inflorescences were selected; these isolates were purified and subsequently transferred to four different culture media: potato sucrose agar (PSA), water agar (WA), potato dextrose agar (PDA) and carnation leaf agar (CLA). The isolates were then submitted to three different lighting regimes: a photoperiod of 12 h (TL), continuous light (CL) and continuous darkness (CD). After seven days of incubation under the three lighting regimes, the mycelial growth was evaluated on PSA, WA and PDA, and sporulation was evaluated on PSA, WA, PDA and CLA. In general, PSA and BDA media support mycelial growth and sporulation, particularly under CL. CLA medium also supports sporulation, particularly under CL. The CD lighting regime and the WA medium are not recommended for either the mycelial growth or sporulation of the F. chlamydosporum species complex. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-02-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142 |
url |
https://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ajaes.ufra.edu.br/index.php/ajaes/article/view/1142/431 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural da Amazônia/UFRA |
publisher.none.fl_str_mv |
Universidade Federal Rural da Amazônia/UFRA |
dc.source.none.fl_str_mv |
Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 57 No 1 (2014): RCA; 35-40 Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 57 n. 1 (2014): RCA; 35-40 2177-8760 1517-591X reponame:Revista de Ciências Agrárias (Belém. Online) instname:Universidade Federal Rural da Amazônia (UFRA) instacron:UFRA |
instname_str |
Universidade Federal Rural da Amazônia (UFRA) |
instacron_str |
UFRA |
institution |
UFRA |
reponame_str |
Revista de Ciências Agrárias (Belém. Online) |
collection |
Revista de Ciências Agrárias (Belém. Online) |
repository.name.fl_str_mv |
Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA) |
repository.mail.fl_str_mv |
allan.lobato@ufra.edu.br || ajaes.suporte@gmail.com |
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1797231627536957440 |