Transferability of microsatellite markers to wild passion fruit species

• Autor(a) principal: Silva, Lucas Pereira da
• Data de Publicação: 2019
• Outros Autores: Marostega, Thalita Neves, Gilio, Thiago Alexandre Santana, Serafim, Milson Evaldo, Araújo, Kelly Lana, Neves, Leonarda Grillo
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Ciências Agrárias (Belém. Online)
• Texto Completo: https://ajaes.ufra.edu.br/index.php/ajaes/article/view/2914
• topic: Plant breeding; Simple sequence repeats; Amplification; Polymorphism; Melhoramento de plantas; Simple sequence repeats; Amplificação; Polimorfismo; Transferibilidade de primers

Molecular markers are tools that allow the monitoring of a breeding program progress. SSR (simple sequence repeats) markers are applied for genetic mapping studies, variability, assisted selection, crossing and backcrossing, because they are codominant, multiallelic and high polymorphic. However, the use of this type of molecular marker is limited, since primers are only available for commercially expressed species. Therefore, this work aimed to evaluate the transferability designed primers of Passiflora alata e Passiflora edulis to four wild species of Passiflora. The species used were: P. cincinnata, P. quadrangularis, P. mucronata and P. nitida. DNA extraction was done through the Promega Wizard® Kit. The amplification protocol used was the one proposed for P. alata and P. edulis with adaptations. Nineteen microsatellites designed for P. edulis and two designed for a genus P. alata were used. The amplicons were taken to horizontal electrophoresis in a 3% agarose gel, followed by staining with Nancy 520 buffer and the revelation was developed in a digital photodocumentator with UV light. The transferability was verified through the evaluation of band presence or absence. The microsatellites loci designed for P. edulis and P. alata were transferable for the examination of at least one species with high variable rate: P. quadrangularis (23.81%), P. cincinnata (33.33%), P. quadrangularis (23.81%), P. mucronata (19.05%) and P. nitida (14.28%). Markers PE13 and PE54 can be selected for molecular characterization studies because they are the only ones able to amplify all the wild passion fruit species tested.