Profile of in vitro-grown aloe plants using different concentrations of 6-benzylaminopurine

• Autor(a) principal: Teixeira Malta, Olival
• Data de Publicação: 2021
• Outros Autores: Vila Verde Souza, Sthefany Hevhanie, de Jesus da Silva de Carvalho, Mariane, Rodrigues Costa, Eva Maria, Ribeiro Mesquita, Paulo Roberto, Bastos de Almeida, Weliton Antônio
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Revista de Ciências Agrárias (Belém. Online)
• Texto Completo: https://ajaes.ufra.edu.br/index.php/ajaes/article/view/3476
• topic: aloe vera; secondary metabolites; active principles; Biotechnology; aloe vera; metabólitos secundários; princípios ativos; Biotecnologia

Aloe plants have a great therapeutic potential, which is attributed to the several bioactive compounds found in their composition. Therefore, the objective of this work was to evaluate the profile of secondary metabolites and the morphophysiological characteristics of Aloe plants collected at different periods and submitted to different growing conditions. So, axillary buds were collected at two different periods and grown in vitro in MS culture medium containing naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP) under controlled conditions for 30 days. Then, the morphophysiological characterization and the analysis of the profile of metabolites produced by the plants were performed (HS-SPME / GC-MS techniques). The results showed that the BAP concentrations did not influence the morphophysiological characteristics of the plants. Almost all plants showed a 100% rooting percentage (RP) when grown in vitro, both with and without BAP in the nutrient medium. Only the plants subjected to BAP concentration of 3 mg L-1 showed a lower RP. The rise in BAP concentrations also promotes a reduction in the total area of ​​volatile organic compounds (VOCs) in plants. Some compounds (hexanal; 2-hexenal; 1-hexanol; 1-nonanol; 2-decanone; beta-ionone) showed a significant reduction (> 0.10%) in their concentration as the concentration of BAP was incremented in the medium cultivation. Nevertheless, the in vitro cultivated plants showed a greater total area and concentration of VOCs than the in vivo cultivated plants.