Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, Brazil
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Ciências Agrárias (Belém. Online) |
Texto Completo: | https://ajaes.ufra.edu.br/index.php/ajaes/article/view/127 |
Resumo: | Toxoplasmosis is a zoonotic protozoan with high potential who own felines, mainly cats as definitive hosts capable of eliminating in your stool, million oocysts of Toxoplasma gondii. These oocysts survive for about a year in the external medium in favorable environment. The aim of this study is to evaluate the presence of specific anti-T IgG antibodies. gondii in 32 serum samples obtained from domestic cats coming in the Metropolitan region Belém do Pará. Owners participants answered a questionnaire on epizootic the feline toxoplasmosis. All samples were tested by enzyme linked immunosorbent assay solid phase through ImmunoComb® Toxo IgG kit. After the serology results were analyzed and associated with epizootic survey. In serological survey, we obtained 100% of negativity in the samples. Epizootic in the survey, it was observed that of the 32 animals, 11 (34.37%) were domiciled, nine (28.12%) and 12 free-living semidomiciliados, four (12.5%) of diurnal and eight (25%) of nocturnal habit. As for food, 11 (34.37%) consumed only food, eight (25%) and feed raw meat, six (18.75%) ration associated with home and seven (21.87%) and raw meat food homemade food. The results suggest that the feline population analyzed, probably due to the creation and food habit system, was not exposed to the parasite T. gondii. |
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Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, BrazilEnsaio imunoenzimático indireto para detecção de anticorpos igg anti-toxoplasma gondii em gatos na cidade de Belém do Pará, BrasilToxoplasmosis is a zoonotic protozoan with high potential who own felines, mainly cats as definitive hosts capable of eliminating in your stool, million oocysts of Toxoplasma gondii. These oocysts survive for about a year in the external medium in favorable environment. The aim of this study is to evaluate the presence of specific anti-T IgG antibodies. gondii in 32 serum samples obtained from domestic cats coming in the Metropolitan region Belém do Pará. Owners participants answered a questionnaire on epizootic the feline toxoplasmosis. All samples were tested by enzyme linked immunosorbent assay solid phase through ImmunoComb® Toxo IgG kit. After the serology results were analyzed and associated with epizootic survey. In serological survey, we obtained 100% of negativity in the samples. Epizootic in the survey, it was observed that of the 32 animals, 11 (34.37%) were domiciled, nine (28.12%) and 12 free-living semidomiciliados, four (12.5%) of diurnal and eight (25%) of nocturnal habit. As for food, 11 (34.37%) consumed only food, eight (25%) and feed raw meat, six (18.75%) ration associated with home and seven (21.87%) and raw meat food homemade food. The results suggest that the feline population analyzed, probably due to the creation and food habit system, was not exposed to the parasite T. gondii.A toxoplasmose é uma protozoose com alto potencial zoonótico que possuem os felídeos,principalmente os gatos, como hospedeiros definitivos capazes de eliminar, em suas fezes, milhões deoocistos do Toxoplasma gondii. Estes oocistos sobrevivem em torno de um ano no meio externo emambiente favorável. O objetivo deste estudo é avaliar a presença de anticorpos IgG específicos anti-T.gondii em 32 amostras de soro obtidas de gatos domésticos procedentes da região Metropolitana deBelém do Pará. Os proprietários participantes responderam a um questionário epizootiológico referenteà toxoplasmose felina. Todas as amostras foram testadas por um ensaio imunoenzimático indireto emfase sólida, através do kit ImmunoComb® II Toxo IgG. Após a sorologia, os resultados foram analisadose associados ao levantamento epizootiológico. Na pesquisa sorológica, obteve-se 100% de negatividade nas amostras. No levantamento epizootiológico, observou-se que dos 32 animais, 11 (34,37%) eram domiciliados, nove (28,12%) de vida livre e 12 semidomiciliados, sendo quatro (12,5%) de hábito diurno e oito (25%) de hábito noturno. Quanto à alimentação, 11 (34,37%) consumiam somente ração,oito (25%) ração e carne crua, seis (18,75%) ração associada à comida caseira e sete (21,87%) comida caseira e carne crua. Os resultados obtidos permitem concluir que, a população felina analisada, provavelmente devido ao sistema de criação e hábito alimentar, não foi exposta ao protozoário T.gondii.Universidade Federal Rural da Amazônia/UFRA2011-03-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/127Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 52 No 1 (2009): RCA; 99-105Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 52 n. 1 (2009): RCA; 99-1052177-87601517-591Xreponame:Revista de Ciências Agrárias (Belém. Online)instname:Universidade Federal Rural da Amazônia (UFRA)instacron:UFRAporhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/127/22Meneses, André Marcelo ConceiçãoNegrão, Karla AlbuquerqueMiranda, Camila FrancoBastos, Renata Kelly GonzagaSouza, Nazaré Fonseca deKuroda, Rosely Bianca dos SantosMoraes, Carla Cristina Guimarães deBenigno, Raimundo Nonato Moraesinfo:eu-repo/semantics/openAccess2018-12-18T18:41:38Zoai:ojs.www.periodicos.ufra.edu.br:article/127Revistahttps://ajaes.ufra.edu.br/index.php/ajaes/PUBhttps://ajaes.ufra.edu.br/index.php/ajaes/oaiallan.lobato@ufra.edu.br || ajaes.suporte@gmail.com2177-87601517-591Xopendoar:2018-12-18T18:41:38Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA)false |
dc.title.none.fl_str_mv |
Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, Brazil Ensaio imunoenzimático indireto para detecção de anticorpos igg anti-toxoplasma gondii em gatos na cidade de Belém do Pará, Brasil |
title |
Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, Brazil |
spellingShingle |
Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, Brazil Meneses, André Marcelo Conceição |
title_short |
Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, Brazil |
title_full |
Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, Brazil |
title_fullStr |
Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, Brazil |
title_full_unstemmed |
Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, Brazil |
title_sort |
Indirect enzyme immunoassay for detection of anti-Toxoplasma gondii IgG antibodies in cats in the city of Belém do Pará, Brazil |
author |
Meneses, André Marcelo Conceição |
author_facet |
Meneses, André Marcelo Conceição Negrão, Karla Albuquerque Miranda, Camila Franco Bastos, Renata Kelly Gonzaga Souza, Nazaré Fonseca de Kuroda, Rosely Bianca dos Santos Moraes, Carla Cristina Guimarães de Benigno, Raimundo Nonato Moraes |
author_role |
author |
author2 |
Negrão, Karla Albuquerque Miranda, Camila Franco Bastos, Renata Kelly Gonzaga Souza, Nazaré Fonseca de Kuroda, Rosely Bianca dos Santos Moraes, Carla Cristina Guimarães de Benigno, Raimundo Nonato Moraes |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Meneses, André Marcelo Conceição Negrão, Karla Albuquerque Miranda, Camila Franco Bastos, Renata Kelly Gonzaga Souza, Nazaré Fonseca de Kuroda, Rosely Bianca dos Santos Moraes, Carla Cristina Guimarães de Benigno, Raimundo Nonato Moraes |
description |
Toxoplasmosis is a zoonotic protozoan with high potential who own felines, mainly cats as definitive hosts capable of eliminating in your stool, million oocysts of Toxoplasma gondii. These oocysts survive for about a year in the external medium in favorable environment. The aim of this study is to evaluate the presence of specific anti-T IgG antibodies. gondii in 32 serum samples obtained from domestic cats coming in the Metropolitan region Belém do Pará. Owners participants answered a questionnaire on epizootic the feline toxoplasmosis. All samples were tested by enzyme linked immunosorbent assay solid phase through ImmunoComb® Toxo IgG kit. After the serology results were analyzed and associated with epizootic survey. In serological survey, we obtained 100% of negativity in the samples. Epizootic in the survey, it was observed that of the 32 animals, 11 (34.37%) were domiciled, nine (28.12%) and 12 free-living semidomiciliados, four (12.5%) of diurnal and eight (25%) of nocturnal habit. As for food, 11 (34.37%) consumed only food, eight (25%) and feed raw meat, six (18.75%) ration associated with home and seven (21.87%) and raw meat food homemade food. The results suggest that the feline population analyzed, probably due to the creation and food habit system, was not exposed to the parasite T. gondii. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-11 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://ajaes.ufra.edu.br/index.php/ajaes/article/view/127 |
url |
https://ajaes.ufra.edu.br/index.php/ajaes/article/view/127 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://ajaes.ufra.edu.br/index.php/ajaes/article/view/127/22 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal Rural da Amazônia/UFRA |
publisher.none.fl_str_mv |
Universidade Federal Rural da Amazônia/UFRA |
dc.source.none.fl_str_mv |
Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 52 No 1 (2009): RCA; 99-105 Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 52 n. 1 (2009): RCA; 99-105 2177-8760 1517-591X reponame:Revista de Ciências Agrárias (Belém. Online) instname:Universidade Federal Rural da Amazônia (UFRA) instacron:UFRA |
instname_str |
Universidade Federal Rural da Amazônia (UFRA) |
instacron_str |
UFRA |
institution |
UFRA |
reponame_str |
Revista de Ciências Agrárias (Belém. Online) |
collection |
Revista de Ciências Agrárias (Belém. Online) |
repository.name.fl_str_mv |
Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA) |
repository.mail.fl_str_mv |
allan.lobato@ufra.edu.br || ajaes.suporte@gmail.com |
_version_ |
1797231626038542336 |