í ?? mitotic index and cell viability of calli of small-murici

Detalhes bibliográficos
Autor(a) principal: Nogueira, Rairys Cravo
Data de Publicação: 2011
Outros Autores: Paiva, Renato, Stein, Vanessa Cristina, Salgado, Caio Césio, Magalhães, Marcelo Murad, Soares, Fernanda Pereira
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Ciências Agrárias (Belém. Online)
Texto Completo: https://ajaes.ufra.edu.br/index.php/ajaes/article/view/51
Resumo: Byrsonima intermedia is a woody and medicinal species occurring in the Brazilian Cerrado. The objective of this research was to determine the pattern of division and viability of cells with embryogenic characteristics. Leaf segments were inoculated on MS medium supplemented with 1.0 mg L-1 2,4-D. Subcultures were performed three times, all at sixty days, resulting in three subcultures. We assessed cell viability and mitotic index of cells with embryogenic characteristics, the second subculture. The second subculture at 20 and 40 days, higher rates of cell division. To 80 days of incubation of calluses, the highest percentage of viable cells with embryogenic characteristics occurs.
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spelling í ?? mitotic index and cell viability of calli of small-muriciíndice mitótico e viabilidade celular de calos embriogênicos de murici-pequenoByrsonima intermedia is a woody and medicinal species occurring in the Brazilian Cerrado. The objective of this research was to determine the pattern of division and viability of cells with embryogenic characteristics. Leaf segments were inoculated on MS medium supplemented with 1.0 mg L-1 2,4-D. Subcultures were performed three times, all at sixty days, resulting in three subcultures. We assessed cell viability and mitotic index of cells with embryogenic characteristics, the second subculture. The second subculture at 20 and 40 days, higher rates of cell division. To 80 days of incubation of calluses, the highest percentage of viable cells with embryogenic characteristics occurs.Byrsonima intermedia é uma espécie lenhosa e medicinal de ocorrência nos Cerrados brasileiros. O objetivo desta pesquisa foi determinar o padrão de divisão e viabilidade de células com características embriogênicas. Segmentos foliares foram inoculados em meio de cultura MS suplementada com 1,0 mg L-1 de 2,4-D. As repicagens foram efetuadas por três vezes, todas aos sessenta dias, resultando em três subcultivos. Avaliou-se a viabilidade celular e o índice mitótico de células com características embriogênicas, no segundo subcultivo. No segundo subcultivo, aos 20 e 40 dias, ocorrem os maiores índices de divisão celular. Até os 80 dias de incubação dos calos, ocorre a maior porcentagem de células viáveis com características embriogênicas.Universidade Federal Rural da Amazônia/UFRA2011-08-19info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/51Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 54 No 1 (2011): RCA; 28 - 32Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 54 n. 1 (2011): RCA; 28 - 322177-87601517-591Xreponame:Revista de Ciências Agrárias (Belém. Online)instname:Universidade Federal Rural da Amazônia (UFRA)instacron:UFRAporhttps://ajaes.ufra.edu.br/index.php/ajaes/article/view/51/175Nogueira, Rairys CravoPaiva, RenatoStein, Vanessa CristinaSalgado, Caio CésioMagalhães, Marcelo MuradSoares, Fernanda Pereirainfo:eu-repo/semantics/openAccess2018-12-18T19:29:09Zoai:ojs.www.periodicos.ufra.edu.br:article/51Revistahttps://ajaes.ufra.edu.br/index.php/ajaes/PUBhttps://ajaes.ufra.edu.br/index.php/ajaes/oaiallan.lobato@ufra.edu.br || ajaes.suporte@gmail.com2177-87601517-591Xopendoar:2018-12-18T19:29:09Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA)false
dc.title.none.fl_str_mv í ?? mitotic index and cell viability of calli of small-murici
índice mitótico e viabilidade celular de calos embriogênicos de murici-pequeno
title í ?? mitotic index and cell viability of calli of small-murici
spellingShingle í ?? mitotic index and cell viability of calli of small-murici
Nogueira, Rairys Cravo
title_short í ?? mitotic index and cell viability of calli of small-murici
title_full í ?? mitotic index and cell viability of calli of small-murici
title_fullStr í ?? mitotic index and cell viability of calli of small-murici
title_full_unstemmed í ?? mitotic index and cell viability of calli of small-murici
title_sort í ?? mitotic index and cell viability of calli of small-murici
author Nogueira, Rairys Cravo
author_facet Nogueira, Rairys Cravo
Paiva, Renato
Stein, Vanessa Cristina
Salgado, Caio Césio
Magalhães, Marcelo Murad
Soares, Fernanda Pereira
author_role author
author2 Paiva, Renato
Stein, Vanessa Cristina
Salgado, Caio Césio
Magalhães, Marcelo Murad
Soares, Fernanda Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Nogueira, Rairys Cravo
Paiva, Renato
Stein, Vanessa Cristina
Salgado, Caio Césio
Magalhães, Marcelo Murad
Soares, Fernanda Pereira
description Byrsonima intermedia is a woody and medicinal species occurring in the Brazilian Cerrado. The objective of this research was to determine the pattern of division and viability of cells with embryogenic characteristics. Leaf segments were inoculated on MS medium supplemented with 1.0 mg L-1 2,4-D. Subcultures were performed three times, all at sixty days, resulting in three subcultures. We assessed cell viability and mitotic index of cells with embryogenic characteristics, the second subculture. The second subculture at 20 and 40 days, higher rates of cell division. To 80 days of incubation of calluses, the highest percentage of viable cells with embryogenic characteristics occurs.
publishDate 2011
dc.date.none.fl_str_mv 2011-08-19
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://ajaes.ufra.edu.br/index.php/ajaes/article/view/51
url https://ajaes.ufra.edu.br/index.php/ajaes/article/view/51
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://ajaes.ufra.edu.br/index.php/ajaes/article/view/51/175
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade Federal Rural da Amazônia/UFRA
publisher.none.fl_str_mv Universidade Federal Rural da Amazônia/UFRA
dc.source.none.fl_str_mv Amazonian Journal of Agricultural Sciences Journal of Agricultural and Environmental Sciences; Vol 54 No 1 (2011): RCA; 28 - 32
Revista de Ciências Agrárias Amazonian Journal of Agricultural and Environmental Sciences; v. 54 n. 1 (2011): RCA; 28 - 32
2177-8760
1517-591X
reponame:Revista de Ciências Agrárias (Belém. Online)
instname:Universidade Federal Rural da Amazônia (UFRA)
instacron:UFRA
instname_str Universidade Federal Rural da Amazônia (UFRA)
instacron_str UFRA
institution UFRA
reponame_str Revista de Ciências Agrárias (Belém. Online)
collection Revista de Ciências Agrárias (Belém. Online)
repository.name.fl_str_mv Revista de Ciências Agrárias (Belém. Online) - Universidade Federal Rural da Amazônia (UFRA)
repository.mail.fl_str_mv allan.lobato@ufra.edu.br || ajaes.suporte@gmail.com
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