MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinical and Biomedical Research |
Texto Completo: | https://seer.ufrgs.br/index.php/hcpa/article/view/91902 |
Resumo: | Introduction: Identification of yeast species has clinical and epidemiological value. Different methods can be used, such as chromogenic media, microculture on corn meal agar with Tween 80, as well as conventional biochemical and automated methods. Recently, proteomic studies employing matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry have been a major advance in diagnosis due to speed of execution and accuracy of results. Methods: For this study, 79 yeast samples were submitted to identification using chromogenic medium, microculture on corn meal-Tween 80 agar, VITEK® 2 Compact identification, and MALDI-TOF mass spectrometry. Results: Most of the 79 samples were identified, with differences in the performance of the methods used. Colonial morphology and microscopy were compatible with the genus Candida. MALDI-TOF mass spectrometry had the best performance, with 78 strains identified (98.7%), compared to VITEK® 2 Compact (92.4%) and microculture on corn meal agar (70.9%). Conclusion: MALDI-TOF mass spectrometry using the VITEK® MS instrument performed best and has proven to be a revolutionary method in clinical microbiology laboratories. Regarding the identification of C. albicans and C. tropicalis, the chromogenic medium had excellent performance, thus being a good option to optimize the process. Keywords: Mass spectrometry; matrix-assisted laser desorption-ionization; candidiasis; Candida; yeasts |
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Clinical and Biomedical Research |
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MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methodsMass spectrometrymatrix-assisted laser desorption-ionizationcandidiasisCandidayeastsYeastsIntroduction: Identification of yeast species has clinical and epidemiological value. Different methods can be used, such as chromogenic media, microculture on corn meal agar with Tween 80, as well as conventional biochemical and automated methods. Recently, proteomic studies employing matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry have been a major advance in diagnosis due to speed of execution and accuracy of results. Methods: For this study, 79 yeast samples were submitted to identification using chromogenic medium, microculture on corn meal-Tween 80 agar, VITEK® 2 Compact identification, and MALDI-TOF mass spectrometry. Results: Most of the 79 samples were identified, with differences in the performance of the methods used. Colonial morphology and microscopy were compatible with the genus Candida. MALDI-TOF mass spectrometry had the best performance, with 78 strains identified (98.7%), compared to VITEK® 2 Compact (92.4%) and microculture on corn meal agar (70.9%). Conclusion: MALDI-TOF mass spectrometry using the VITEK® MS instrument performed best and has proven to be a revolutionary method in clinical microbiology laboratories. Regarding the identification of C. albicans and C. tropicalis, the chromogenic medium had excellent performance, thus being a good option to optimize the process. Keywords: Mass spectrometry; matrix-assisted laser desorption-ionization; candidiasis; Candida; yeastsHCPA/FAMED/UFRGS2019-08-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed ArticleAvaliado por Paresapplication/pdfhttps://seer.ufrgs.br/index.php/hcpa/article/view/91902Clinical & Biomedical Research; Vol. 39 No. 2 (2019): Clinical and Biomedical ResearchClinical and Biomedical Research; v. 39 n. 2 (2019): Clinical and Biomedical Research2357-9730reponame:Clinical and Biomedical Researchinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSenghttps://seer.ufrgs.br/index.php/hcpa/article/view/91902/pdfCopyright (c) 2019 Clinical and Biomedical Researchinfo:eu-repo/semantics/openAccessAraújo, Max Roberto BatistaSeabra, Luisa FerreiraBernardes Sousa, Mireille Ângela2024-01-19T14:22:12Zoai:seer.ufrgs.br:article/91902Revistahttps://www.seer.ufrgs.br/index.php/hcpaPUBhttps://seer.ufrgs.br/index.php/hcpa/oai||cbr@hcpa.edu.br2357-97302357-9730opendoar:2024-01-19T14:22:12Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.none.fl_str_mv |
MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods |
title |
MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods |
spellingShingle |
MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods Araújo, Max Roberto Batista Mass spectrometry matrix-assisted laser desorption-ionization candidiasis Candida yeasts Yeasts |
title_short |
MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods |
title_full |
MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods |
title_fullStr |
MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods |
title_full_unstemmed |
MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods |
title_sort |
MALDI-TOF mass spectrometry identification of yeast-form fungi: A comparison between methods |
author |
Araújo, Max Roberto Batista |
author_facet |
Araújo, Max Roberto Batista Seabra, Luisa Ferreira Bernardes Sousa, Mireille Ângela |
author_role |
author |
author2 |
Seabra, Luisa Ferreira Bernardes Sousa, Mireille Ângela |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Araújo, Max Roberto Batista Seabra, Luisa Ferreira Bernardes Sousa, Mireille Ângela |
dc.subject.por.fl_str_mv |
Mass spectrometry matrix-assisted laser desorption-ionization candidiasis Candida yeasts Yeasts |
topic |
Mass spectrometry matrix-assisted laser desorption-ionization candidiasis Candida yeasts Yeasts |
description |
Introduction: Identification of yeast species has clinical and epidemiological value. Different methods can be used, such as chromogenic media, microculture on corn meal agar with Tween 80, as well as conventional biochemical and automated methods. Recently, proteomic studies employing matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry have been a major advance in diagnosis due to speed of execution and accuracy of results. Methods: For this study, 79 yeast samples were submitted to identification using chromogenic medium, microculture on corn meal-Tween 80 agar, VITEK® 2 Compact identification, and MALDI-TOF mass spectrometry. Results: Most of the 79 samples were identified, with differences in the performance of the methods used. Colonial morphology and microscopy were compatible with the genus Candida. MALDI-TOF mass spectrometry had the best performance, with 78 strains identified (98.7%), compared to VITEK® 2 Compact (92.4%) and microculture on corn meal agar (70.9%). Conclusion: MALDI-TOF mass spectrometry using the VITEK® MS instrument performed best and has proven to be a revolutionary method in clinical microbiology laboratories. Regarding the identification of C. albicans and C. tropicalis, the chromogenic medium had excellent performance, thus being a good option to optimize the process. Keywords: Mass spectrometry; matrix-assisted laser desorption-ionization; candidiasis; Candida; yeasts |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-08-20 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Avaliado por Pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/91902 |
url |
https://seer.ufrgs.br/index.php/hcpa/article/view/91902 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/91902/pdf |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Clinical and Biomedical Research info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Clinical and Biomedical Research |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
dc.source.none.fl_str_mv |
Clinical & Biomedical Research; Vol. 39 No. 2 (2019): Clinical and Biomedical Research Clinical and Biomedical Research; v. 39 n. 2 (2019): Clinical and Biomedical Research 2357-9730 reponame:Clinical and Biomedical Research instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Clinical and Biomedical Research |
collection |
Clinical and Biomedical Research |
repository.name.fl_str_mv |
Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
||cbr@hcpa.edu.br |
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1799767054724628480 |