Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinical and Biomedical Research |
Texto Completo: | https://seer.ufrgs.br/index.php/hcpa/article/view/48676 |
Resumo: | Introduction: Mouse models of emphysema are important tools for testing different therapeutic strategies. The aim of this study was to develop a mouse model of emphysema induced by different doses of elastase in order to produce different degrees of severity. Methods: Thirty female mice (C57BL/6) were used in this study. Different doses of porcine pancreatic elastase were administered intratracheally once a week for four weeks, as follows: 0.1 U (n=8), 0.15 U (n=7), and 0.2 U (n=7). Control mice (n=8) received 50 microL of sterile saline solution intratracheally. Lung mechanics were analyzed by plethysmography. Mean linear intercept and volume fraction occupied by collagen and elastic fibers were determined. Results: An increase in lung resistance was observed with 0.2 U of elastase [median (P-25-P75): 2.02 (1.67; 2.34) cmH2O.s/mL], as well as a decrease in tidal volume and minute ventilation. Peak expiratory flow increased significantly in the groups treated with 0.15 U and 0.2 U of elastase. Mean linear intercept was higher with 0.15 U and 0.2 U of elastase, with destruction of alveolar walls [median (P-25-P75): 30.31 (26.65-43.13) microm and 49.49 (31.67-57.71) microm respectively]. The volume fraction occupied by collagen and elastic fibers was lower in the group receiving 0.2 U of elastase. Conclusion: Four intratracheal instillations of 0.2 U of elastase once a week induced changes in lung function and histology, producing an experimental model of severe pulmonary emphysema, whereas 0.15 U resulted in only histological changes. |
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Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental studyDevelopment of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental studyenfisema pulmonar, mecânica pulmonar, elastase pancreática.Pulmonary emphysemaLung mechanicsPancreatic elastase.Introduction: Mouse models of emphysema are important tools for testing different therapeutic strategies. The aim of this study was to develop a mouse model of emphysema induced by different doses of elastase in order to produce different degrees of severity. Methods: Thirty female mice (C57BL/6) were used in this study. Different doses of porcine pancreatic elastase were administered intratracheally once a week for four weeks, as follows: 0.1 U (n=8), 0.15 U (n=7), and 0.2 U (n=7). Control mice (n=8) received 50 microL of sterile saline solution intratracheally. Lung mechanics were analyzed by plethysmography. Mean linear intercept and volume fraction occupied by collagen and elastic fibers were determined. Results: An increase in lung resistance was observed with 0.2 U of elastase [median (P-25-P75): 2.02 (1.67; 2.34) cmH2O.s/mL], as well as a decrease in tidal volume and minute ventilation. Peak expiratory flow increased significantly in the groups treated with 0.15 U and 0.2 U of elastase. Mean linear intercept was higher with 0.15 U and 0.2 U of elastase, with destruction of alveolar walls [median (P-25-P75): 30.31 (26.65-43.13) microm and 49.49 (31.67-57.71) microm respectively]. The volume fraction occupied by collagen and elastic fibers was lower in the group receiving 0.2 U of elastase. Conclusion: Four intratracheal instillations of 0.2 U of elastase once a week induced changes in lung function and histology, producing an experimental model of severe pulmonary emphysema, whereas 0.15 U resulted in only histological changes.Introduction: Mouse models of emphysema are important tools for testing different therapeutic strategies. The aim of this study was to develop a mouse model of emphysema induced by different doses of elastase in order to produce different degrees of severity.Methods: Thirty female mice (C57BL/6) were used in this study. Different doses of porcine pancreatic elastase were administered intratracheally once a week for four weeks, as follows: 0.1 U (n=8), 0.15 U (n=7), and 0.2 U (n=7). Control mice (n=8) received 50 microL of sterile saline solution intratracheally. Lung mechanics were analyzed by plethysmography. Mean linear intercept and volume fraction occupied by collagen and elastic fibers were determined.Results: An increase in lung resistance was observed with 0.2 U of elastase [median (P-25-P75): 2.02 (1.67; 2.34) cmH2O.s/mL], as well as a decrease in tidal volume and minute ventilation. Peak expiratory flow increased significantly in the groups treated with 0.15 U and 0.2 U of elastase. Mean linear intercept was higher with 0.15 U and 0.2 U of elastase, with destruction of alveolar walls [median (P-25-P75): 30.31 (26.65-43.13) microm and 49.49 (31.67-57.71) microm respectively]. The volume fraction occupied by collagen and elastic fibers was lower in the group receiving 0.2 U of elastase.Conclusion: Four intratracheal instillations of 0.2 U of elastase once a week induced changes in lung function and histology, producing an experimental model of severe pulmonary emphysema, whereas 0.15 U resulted in only histological changes.HCPA/FAMED/UFRGS2014-10-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed ArticleAvaliado por Paresapplication/pdfhttps://seer.ufrgs.br/index.php/hcpa/article/view/48676Clinical & Biomedical Research; Vol. 34 No. 3 (2014): Clinical and Biomedical ResearchClinical and Biomedical Research; v. 34 n. 3 (2014): Clinical and Biomedical Research2357-9730reponame:Clinical and Biomedical Researchinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSenghttps://seer.ufrgs.br/index.php/hcpa/article/view/48676/31850Palma Kuhl, CristianaAlves Garcez, Tuane NerissaBileski Magrisso, AlessandraFernandes Correia Laurino, Claudia CileneObino Cirne-Lima, ElizabethRieken Macedo Rocco, PatriciaVieira de Macedo Neto, Amarilioinfo:eu-repo/semantics/openAccess2024-01-19T13:30:54Zoai:seer.ufrgs.br:article/48676Revistahttps://www.seer.ufrgs.br/index.php/hcpaPUBhttps://seer.ufrgs.br/index.php/hcpa/oai||cbr@hcpa.edu.br2357-97302357-9730opendoar:2024-01-19T13:30:54Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.none.fl_str_mv |
Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental studyenfisema pulmonar, mecânica pulmonar, elastase pancreática. |
title |
Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study |
spellingShingle |
Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study Palma Kuhl, Cristiana Pulmonary emphysema Lung mechanics Pancreatic elastase. |
title_short |
Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study |
title_full |
Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study |
title_fullStr |
Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study |
title_full_unstemmed |
Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study |
title_sort |
Development of different degrees of elastase-induced emphysema in mice: a randomized controlled experimental study |
author |
Palma Kuhl, Cristiana |
author_facet |
Palma Kuhl, Cristiana Alves Garcez, Tuane Nerissa Bileski Magrisso, Alessandra Fernandes Correia Laurino, Claudia Cilene Obino Cirne-Lima, Elizabeth Rieken Macedo Rocco, Patricia Vieira de Macedo Neto, Amarilio |
author_role |
author |
author2 |
Alves Garcez, Tuane Nerissa Bileski Magrisso, Alessandra Fernandes Correia Laurino, Claudia Cilene Obino Cirne-Lima, Elizabeth Rieken Macedo Rocco, Patricia Vieira de Macedo Neto, Amarilio |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Palma Kuhl, Cristiana Alves Garcez, Tuane Nerissa Bileski Magrisso, Alessandra Fernandes Correia Laurino, Claudia Cilene Obino Cirne-Lima, Elizabeth Rieken Macedo Rocco, Patricia Vieira de Macedo Neto, Amarilio |
dc.subject.por.fl_str_mv |
Pulmonary emphysema Lung mechanics Pancreatic elastase. |
topic |
Pulmonary emphysema Lung mechanics Pancreatic elastase. |
description |
Introduction: Mouse models of emphysema are important tools for testing different therapeutic strategies. The aim of this study was to develop a mouse model of emphysema induced by different doses of elastase in order to produce different degrees of severity. Methods: Thirty female mice (C57BL/6) were used in this study. Different doses of porcine pancreatic elastase were administered intratracheally once a week for four weeks, as follows: 0.1 U (n=8), 0.15 U (n=7), and 0.2 U (n=7). Control mice (n=8) received 50 microL of sterile saline solution intratracheally. Lung mechanics were analyzed by plethysmography. Mean linear intercept and volume fraction occupied by collagen and elastic fibers were determined. Results: An increase in lung resistance was observed with 0.2 U of elastase [median (P-25-P75): 2.02 (1.67; 2.34) cmH2O.s/mL], as well as a decrease in tidal volume and minute ventilation. Peak expiratory flow increased significantly in the groups treated with 0.15 U and 0.2 U of elastase. Mean linear intercept was higher with 0.15 U and 0.2 U of elastase, with destruction of alveolar walls [median (P-25-P75): 30.31 (26.65-43.13) microm and 49.49 (31.67-57.71) microm respectively]. The volume fraction occupied by collagen and elastic fibers was lower in the group receiving 0.2 U of elastase. Conclusion: Four intratracheal instillations of 0.2 U of elastase once a week induced changes in lung function and histology, producing an experimental model of severe pulmonary emphysema, whereas 0.15 U resulted in only histological changes. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion Peer-reviewed Article Avaliado por Pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/48676 |
url |
https://seer.ufrgs.br/index.php/hcpa/article/view/48676 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://seer.ufrgs.br/index.php/hcpa/article/view/48676/31850 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
publisher.none.fl_str_mv |
HCPA/FAMED/UFRGS |
dc.source.none.fl_str_mv |
Clinical & Biomedical Research; Vol. 34 No. 3 (2014): Clinical and Biomedical Research Clinical and Biomedical Research; v. 34 n. 3 (2014): Clinical and Biomedical Research 2357-9730 reponame:Clinical and Biomedical Research instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Clinical and Biomedical Research |
collection |
Clinical and Biomedical Research |
repository.name.fl_str_mv |
Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
||cbr@hcpa.edu.br |
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1799767053658226688 |