MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSIS

Detalhes bibliográficos
Autor(a) principal: Oliveira, Isabel
Data de Publicação: 2022
Outros Autores: D’Avila, Angela, S Brum, Ilma, Spritzer, Poli Mara
Tipo de documento: Artigo
Idioma: por
Título da fonte: Clinical and Biomedical Research
Texto Completo: https://seer.ufrgs.br/index.php/hcpa/article/view/126200
Resumo: Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR), type 1 and type 2 5a-reductase isoenzymes (5a R1 and 2) and type 2 17b hydroxysteroid dehydrogenase (17b-HSD 2) in plucked scalp hairs from hirsute patients and normal subjects. We studied 33 women with hirsutism [20 with polycystic ovary syndrome (PCOS), 13 with idiopathic hirsutism (IH)]; 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman-Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and enzymes mRNA levels were estimated by reverse transcriptionpolymerase chain reaction (RT-PCR). AR expression was similar in all groups. The gene expression of 5a R2 was not detected in any hair samples analyzed in this study. No differences were found on 5a R1 mRNA levels between men and normal women (0.78 ± 0.05 vs. 0.74 ± 0.06, respectively). 5a R1 gene expression in the plucked hair cells from scalp of normal women (0.85 ± 0.04), PCOS (0.78 ± 0.05) and IH (0.80 ± 0.06) was also similar. 17b-HSD2 gene expression in hirsute patients was lower (2.2±0.13 and 2.0±0.15, for PCOS and IH, respectively) than in normal women (3.1±0.17, p<0.05), and similar to men (1.8±0.22). In conclusion, these results indicate that there are no changes on 5a R1 gene expression in the plucked hair cells from scalp, related to gender or hirsutism. The lower expression of 17b-HSD2 mRNA in scalp hairs of hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men.
id UFRGS-20_b169817718275caad8f926db1d415588
oai_identifier_str oai:seer.ufrgs.br:article/126200
network_acronym_str UFRGS-20
network_name_str Clinical and Biomedical Research
repository_id_str
spelling MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSISMECANISMOS MOLECULARES NO HIRSUTISMO: EXPRESSÃO GÊNICA DE ENZIMAS DO METABOLISMO ANDROGÊNICO NO FOLÍCULO PILOSO E ASSOCIAÇÃO COM O DIAGNÓSTICO ETIOLÓGICOandrogen receptorhair folliclehirsutism17b hydroxysteroid dehydrogenase5areductasepolycystic ovary syndromeAndrogens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR), type 1 and type 2 5a-reductase isoenzymes (5a R1 and 2) and type 2 17b hydroxysteroid dehydrogenase (17b-HSD 2) in plucked scalp hairs from hirsute patients and normal subjects. We studied 33 women with hirsutism [20 with polycystic ovary syndrome (PCOS), 13 with idiopathic hirsutism (IH)]; 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman-Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and enzymes mRNA levels were estimated by reverse transcriptionpolymerase chain reaction (RT-PCR). AR expression was similar in all groups. The gene expression of 5a R2 was not detected in any hair samples analyzed in this study. No differences were found on 5a R1 mRNA levels between men and normal women (0.78 ± 0.05 vs. 0.74 ± 0.06, respectively). 5a R1 gene expression in the plucked hair cells from scalp of normal women (0.85 ± 0.04), PCOS (0.78 ± 0.05) and IH (0.80 ± 0.06) was also similar. 17b-HSD2 gene expression in hirsute patients was lower (2.2±0.13 and 2.0±0.15, for PCOS and IH, respectively) than in normal women (3.1±0.17, p<0.05), and similar to men (1.8±0.22). In conclusion, these results indicate that there are no changes on 5a R1 gene expression in the plucked hair cells from scalp, related to gender or hirsutism. The lower expression of 17b-HSD2 mRNA in scalp hairs of hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men.Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR), type 1 and type 2 5a-reductase isoenzymes (5a R1 and 2) and type 2 17b hydroxysteroid dehydrogenase (17b-HSD 2) in plucked scalp hairs from hirsute patients and normal subjects. We studied 33 women with hirsutism [20 with polycystic ovary syndrome (PCOS), 13 with idiopathic hirsutism (IH)]; 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman-Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and enzymes mRNA levels were estimated by reverse transcriptionpolymerase chain reaction (RT-PCR). AR expression was similar in all groups. The gene expression of 5a R2 was not detected in any hair samples analyzed in this study. No differences were found on 5a R1 mRNA levels between men and normal women (0.78 ± 0.05 vs. 0.74 ± 0.06, respectively). 5a R1 gene expression in the plucked hair cells from scalp of normal women (0.85 ± 0.04), PCOS (0.78 ± 0.05) and IH (0.80 ± 0.06) was also similar. 17b-HSD2 gene expression in hirsute patients was lower (2.2±0.13 and 2.0±0.15, for PCOS and IH, respectively) than in normal women (3.1±0.17, p<0.05), and similar to men (1.8±0.22). In conclusion, these results indicate that there are no changes on 5a R1 gene expression in the plucked hair cells from scalp, related to gender or hirsutism. The lower expression of 17b-HSD2 mRNA in scalp hairs of hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men.HCPA/FAMED/UFRGS2022-09-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionPeer-reviewed ArticleAvaliado por Paresapplication/pdfhttps://seer.ufrgs.br/index.php/hcpa/article/view/126200Clinical & Biomedical Research; Vol. 23 No. 1 - 2 (2003): Revista HCPAClinical and Biomedical Research; v. 23 n. 1 - 2 (2003): Revista HCPA2357-9730reponame:Clinical and Biomedical Researchinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSporhttps://seer.ufrgs.br/index.php/hcpa/article/view/126200/85684Copyright (c) 2022 Isabel O Oliveira, Angela D’Avila, Ilma S Brum, Poli Mara Spritzerhttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessOliveira, IsabelD’Avila, Angela S Brum, Ilma Spritzer, Poli Mara 2022-09-27T20:38:15Zoai:seer.ufrgs.br:article/126200Revistahttps://www.seer.ufrgs.br/index.php/hcpaPUBhttps://seer.ufrgs.br/index.php/hcpa/oai||cbr@hcpa.edu.br2357-97302357-9730opendoar:2022-09-27T20:38:15Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.none.fl_str_mv MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSIS
MECANISMOS MOLECULARES NO HIRSUTISMO: EXPRESSÃO GÊNICA DE ENZIMAS DO METABOLISMO ANDROGÊNICO NO FOLÍCULO PILOSO E ASSOCIAÇÃO COM O DIAGNÓSTICO ETIOLÓGICO
title MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSIS
spellingShingle MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSIS
Oliveira, Isabel
androgen receptor
hair follicle
hirsutism
17b hydroxysteroid dehydrogenase
5areductase
polycystic ovary syndrome
title_short MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSIS
title_full MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSIS
title_fullStr MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSIS
title_full_unstemmed MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSIS
title_sort MOLECULAR MECHANISMS ON HIRSUTISM: GENE EXPRESSION OF ANDROGEN METABOLIZING ENZYMES IN SCALP HAIR AND ASSOCIATION WITH ETIOLOGICAL DIAGNOSIS
author Oliveira, Isabel
author_facet Oliveira, Isabel
D’Avila, Angela
S Brum, Ilma
Spritzer, Poli Mara
author_role author
author2 D’Avila, Angela
S Brum, Ilma
Spritzer, Poli Mara
author2_role author
author
author
dc.contributor.author.fl_str_mv Oliveira, Isabel
D’Avila, Angela
S Brum, Ilma
Spritzer, Poli Mara
dc.subject.por.fl_str_mv androgen receptor
hair follicle
hirsutism
17b hydroxysteroid dehydrogenase
5areductase
polycystic ovary syndrome
topic androgen receptor
hair follicle
hirsutism
17b hydroxysteroid dehydrogenase
5areductase
polycystic ovary syndrome
description Androgens are the main hormonal regulators of human hair growth and they are related to clinical conditions such as hirsutism. The aim of this study was to analyze the gene expression of androgen receptor (AR), type 1 and type 2 5a-reductase isoenzymes (5a R1 and 2) and type 2 17b hydroxysteroid dehydrogenase (17b-HSD 2) in plucked scalp hairs from hirsute patients and normal subjects. We studied 33 women with hirsutism [20 with polycystic ovary syndrome (PCOS), 13 with idiopathic hirsutism (IH)]; 15 control women; and 10 control men. Hirsutism was assessed by a modified Ferriman-Gallwey method. Hormonal status was assessed between days 2 and 10 of the menstrual cycle or on any day when the patients were amenorrheic. AR and enzymes mRNA levels were estimated by reverse transcriptionpolymerase chain reaction (RT-PCR). AR expression was similar in all groups. The gene expression of 5a R2 was not detected in any hair samples analyzed in this study. No differences were found on 5a R1 mRNA levels between men and normal women (0.78 ± 0.05 vs. 0.74 ± 0.06, respectively). 5a R1 gene expression in the plucked hair cells from scalp of normal women (0.85 ± 0.04), PCOS (0.78 ± 0.05) and IH (0.80 ± 0.06) was also similar. 17b-HSD2 gene expression in hirsute patients was lower (2.2±0.13 and 2.0±0.15, for PCOS and IH, respectively) than in normal women (3.1±0.17, p<0.05), and similar to men (1.8±0.22). In conclusion, these results indicate that there are no changes on 5a R1 gene expression in the plucked hair cells from scalp, related to gender or hirsutism. The lower expression of 17b-HSD2 mRNA in scalp hairs of hirsute patients suggests androgen metabolism disturbances with predominance of more potent androgens, as occurs in men.
publishDate 2022
dc.date.none.fl_str_mv 2022-09-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Peer-reviewed Article
Avaliado por Pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://seer.ufrgs.br/index.php/hcpa/article/view/126200
url https://seer.ufrgs.br/index.php/hcpa/article/view/126200
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://seer.ufrgs.br/index.php/hcpa/article/view/126200/85684
dc.rights.driver.fl_str_mv Copyright (c) 2022 Isabel O Oliveira, Angela D’Avila, Ilma S Brum, Poli Mara Spritzer
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Isabel O Oliveira, Angela D’Avila, Ilma S Brum, Poli Mara Spritzer
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv HCPA/FAMED/UFRGS
publisher.none.fl_str_mv HCPA/FAMED/UFRGS
dc.source.none.fl_str_mv Clinical & Biomedical Research; Vol. 23 No. 1 - 2 (2003): Revista HCPA
Clinical and Biomedical Research; v. 23 n. 1 - 2 (2003): Revista HCPA
2357-9730
reponame:Clinical and Biomedical Research
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Clinical and Biomedical Research
collection Clinical and Biomedical Research
repository.name.fl_str_mv Clinical and Biomedical Research - Universidade Federal do Rio Grande do Sul (UFRGS)
repository.mail.fl_str_mv ||cbr@hcpa.edu.br
_version_ 1799767057552637952

Registros relacionados