Intraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brain
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225868 |
Resumo: | Even though technologies involving nano/microparticles have great potential, it is crucial to determine possible toxicity of these technological products before extensive use. Fullerenes C60 are nanomaterials with unique physicochemical and biological properties that are important for the development of many technological applications. The aim of this study was to evaluate the consequences of nonphotoexcited fullerene C60 exposure in brain acetylcholinesterase expression and activity, antioxidant responses, and oxidative damage using adult zebrafish as an animal model. None of the doses tested (7.5, 15, and 30 mg/kg) altered AChE activity, antioxidant responses, and oxidative damage when zebrafish were exposed to nonphotoexcited C60 nano/microparticles during 6 and 12 hours. However, adult zebrafish exposed to the 30 mg/kg dose for 24 hours have shown enhanced AChE activity and augmented lipid peroxidation (TBARS assays) in brain. In addition, the up-regulation of brain AChE activity was neither related to the transcriptional control (RT-qPCR analysis) nor to the direct action of nonphotoexcited C60 nano/microparticles on the protein (in vitro results) but probably involved a posttranscriptional or posttranslational modulation of this enzymatic activity. Taken together these findings provided further evidence of toxic effects on brain after C60 exposure. |
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Dal Forno, Gonzalo OgliariKist, Luiza WilgesAzevedo, Mariana Barbieri deFritsch, Rachel SeemannPereira, Talita Carneiro BrandaoBritto, Roberta SocoowskiGuterres, Silvia StanisçuaskiKülkamp-Guerreiro, Irene ClemesBonan, Carla DeniseMonserrat, José MariaBogo, Mauricio Reis2021-08-18T04:47:56Z20131110-7243http://hdl.handle.net/10183/225868000891814Even though technologies involving nano/microparticles have great potential, it is crucial to determine possible toxicity of these technological products before extensive use. Fullerenes C60 are nanomaterials with unique physicochemical and biological properties that are important for the development of many technological applications. The aim of this study was to evaluate the consequences of nonphotoexcited fullerene C60 exposure in brain acetylcholinesterase expression and activity, antioxidant responses, and oxidative damage using adult zebrafish as an animal model. None of the doses tested (7.5, 15, and 30 mg/kg) altered AChE activity, antioxidant responses, and oxidative damage when zebrafish were exposed to nonphotoexcited C60 nano/microparticles during 6 and 12 hours. However, adult zebrafish exposed to the 30 mg/kg dose for 24 hours have shown enhanced AChE activity and augmented lipid peroxidation (TBARS assays) in brain. In addition, the up-regulation of brain AChE activity was neither related to the transcriptional control (RT-qPCR analysis) nor to the direct action of nonphotoexcited C60 nano/microparticles on the protein (in vitro results) but probably involved a posttranscriptional or posttranslational modulation of this enzymatic activity. Taken together these findings provided further evidence of toxic effects on brain after C60 exposure.application/pdfengJournal of biomedicine and biotechnology. Cairo. Vol. 2013, p. 1-11NanotecnologiaNanopartículasToxicologiaFulerenosIntraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brainEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000891814.pdf.txt000891814.pdf.txtExtracted Texttext/plain50756http://www.lume.ufrgs.br/bitstream/10183/225868/2/000891814.pdf.txt074cd495b824581f8d2a47d78404fda4MD52ORIGINAL000891814.pdfTexto completo (inglês)application/pdf1784567http://www.lume.ufrgs.br/bitstream/10183/225868/1/000891814.pdf0558b3c20f5ba57f523bf5bf75cb551eMD5110183/2258682021-08-18 05:21:40.953oai:www.lume.ufrgs.br:10183/225868Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T08:21:40Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Intraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brain |
title |
Intraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brain |
spellingShingle |
Intraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brain Dal Forno, Gonzalo Ogliari Nanotecnologia Nanopartículas Toxicologia Fulerenos |
title_short |
Intraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brain |
title_full |
Intraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brain |
title_fullStr |
Intraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brain |
title_full_unstemmed |
Intraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brain |
title_sort |
Intraperitoneal exposure to nano/microparticles of fullerene (C60) increases acetylcholinesterase activity and lipid peroxidation in adult zebrafish (danio rerio) brain |
author |
Dal Forno, Gonzalo Ogliari |
author_facet |
Dal Forno, Gonzalo Ogliari Kist, Luiza Wilges Azevedo, Mariana Barbieri de Fritsch, Rachel Seemann Pereira, Talita Carneiro Brandao Britto, Roberta Socoowski Guterres, Silvia Stanisçuaski Külkamp-Guerreiro, Irene Clemes Bonan, Carla Denise Monserrat, José Maria Bogo, Mauricio Reis |
author_role |
author |
author2 |
Kist, Luiza Wilges Azevedo, Mariana Barbieri de Fritsch, Rachel Seemann Pereira, Talita Carneiro Brandao Britto, Roberta Socoowski Guterres, Silvia Stanisçuaski Külkamp-Guerreiro, Irene Clemes Bonan, Carla Denise Monserrat, José Maria Bogo, Mauricio Reis |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Dal Forno, Gonzalo Ogliari Kist, Luiza Wilges Azevedo, Mariana Barbieri de Fritsch, Rachel Seemann Pereira, Talita Carneiro Brandao Britto, Roberta Socoowski Guterres, Silvia Stanisçuaski Külkamp-Guerreiro, Irene Clemes Bonan, Carla Denise Monserrat, José Maria Bogo, Mauricio Reis |
dc.subject.por.fl_str_mv |
Nanotecnologia Nanopartículas Toxicologia Fulerenos |
topic |
Nanotecnologia Nanopartículas Toxicologia Fulerenos |
description |
Even though technologies involving nano/microparticles have great potential, it is crucial to determine possible toxicity of these technological products before extensive use. Fullerenes C60 are nanomaterials with unique physicochemical and biological properties that are important for the development of many technological applications. The aim of this study was to evaluate the consequences of nonphotoexcited fullerene C60 exposure in brain acetylcholinesterase expression and activity, antioxidant responses, and oxidative damage using adult zebrafish as an animal model. None of the doses tested (7.5, 15, and 30 mg/kg) altered AChE activity, antioxidant responses, and oxidative damage when zebrafish were exposed to nonphotoexcited C60 nano/microparticles during 6 and 12 hours. However, adult zebrafish exposed to the 30 mg/kg dose for 24 hours have shown enhanced AChE activity and augmented lipid peroxidation (TBARS assays) in brain. In addition, the up-regulation of brain AChE activity was neither related to the transcriptional control (RT-qPCR analysis) nor to the direct action of nonphotoexcited C60 nano/microparticles on the protein (in vitro results) but probably involved a posttranscriptional or posttranslational modulation of this enzymatic activity. Taken together these findings provided further evidence of toxic effects on brain after C60 exposure. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2021-08-18T04:47:56Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/225868 |
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1110-7243 |
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000891814 |
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http://hdl.handle.net/10183/225868 |
dc.language.iso.fl_str_mv |
eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of biomedicine and biotechnology. Cairo. Vol. 2013, p. 1-11 |
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