Seizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterization
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225321 |
Resumo: | Pentylenetetrazole (PTZ) is a common convulsant agent used in animal models to investigate the mechanisms of seizures. Although adult zebrafish have been recently used to study epileptic seizures, a thorough characterization of the PTZinduced seizures in this animal model is missing. The goal of this study was to perform a detailed temporal behavior profile characterization of PTZ-induced seizure in adult zebrafish. The behavioral profile during 20 min of PTZ immersion (5, 7.5, 10, and 15 mM) was characterized by stages defined as scores: (0) short swim, (1) increased swimming activity and high frequency of opercular movement, (2) erratic movements, (3) circular movements, (4) clonic seizure-like behavior, (5) fall to the bottom of the tank and tonic seizure-like behavior, (6) death. Animals exposed to distinct PTZ concentrations presented different seizure profiles, intensities and latencies to reach all scores. Only animals immersed into 15 mM PTZ showed an increased time to return to the normal behavior (score 0), after exposure. Total mortality rate at 10 and 15 mM were 33% and 50%, respectively. Considering all behavioral parameters, 5, 7.5, 10, and 15 mM PTZ, induced seizures with low, intermediate, and high severity, respectively. Pretreatment with diazepam (DZP) significantly attenuated seizure severity. Finally, the brain PTZ levels in adult zebrafish immersed into the chemoconvulsant solution at 5 and 10 mM were comparable to those described for the rodent model, with a peak after a 20-min of exposure. The PTZ brain levels observed after 2.5-min PTZ exposure and after 60-min removal from exposure were similar. Altogether, our results showed a detailed temporal behavioral characterization of a PTZ epileptic seizure model in adult zebrafish. These behavioral analyses and the simple method for PTZ quantification could be considered as important tools for future investigations and translational research. |
id |
UFRGS-2_02012bfec5fa25b857fe9e34d5680a60 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/225321 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Mussulini, Ben Hur MarinsLeite, Carlos EduardoZenki, Kamila GagliariMoro, LuanaBaggio, SuelenRico, Eduardo PachecoRosemberg, Denis BroockDias, Renato DutraSouza, Tadeu Mello eCalcagnotto, Maria ElisaCampos, Maria MarthaBattastini, Ana Maria OliveiraOliveira, Diogo Losch de2021-08-06T04:42:49Z20131932-6203http://hdl.handle.net/10183/225321000876721Pentylenetetrazole (PTZ) is a common convulsant agent used in animal models to investigate the mechanisms of seizures. Although adult zebrafish have been recently used to study epileptic seizures, a thorough characterization of the PTZinduced seizures in this animal model is missing. The goal of this study was to perform a detailed temporal behavior profile characterization of PTZ-induced seizure in adult zebrafish. The behavioral profile during 20 min of PTZ immersion (5, 7.5, 10, and 15 mM) was characterized by stages defined as scores: (0) short swim, (1) increased swimming activity and high frequency of opercular movement, (2) erratic movements, (3) circular movements, (4) clonic seizure-like behavior, (5) fall to the bottom of the tank and tonic seizure-like behavior, (6) death. Animals exposed to distinct PTZ concentrations presented different seizure profiles, intensities and latencies to reach all scores. Only animals immersed into 15 mM PTZ showed an increased time to return to the normal behavior (score 0), after exposure. Total mortality rate at 10 and 15 mM were 33% and 50%, respectively. Considering all behavioral parameters, 5, 7.5, 10, and 15 mM PTZ, induced seizures with low, intermediate, and high severity, respectively. Pretreatment with diazepam (DZP) significantly attenuated seizure severity. Finally, the brain PTZ levels in adult zebrafish immersed into the chemoconvulsant solution at 5 and 10 mM were comparable to those described for the rodent model, with a peak after a 20-min of exposure. The PTZ brain levels observed after 2.5-min PTZ exposure and after 60-min removal from exposure were similar. Altogether, our results showed a detailed temporal behavioral characterization of a PTZ epileptic seizure model in adult zebrafish. These behavioral analyses and the simple method for PTZ quantification could be considered as important tools for future investigations and translational research.application/pdfengPLoS ONE. San Francisco. Vol. 8, no. 1 (Jan. 2013), e54515, 9 f.ConvulsõesPentilenotetrazolPeixe-zebraSeizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterizationEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000876721.pdf.txt000876721.pdf.txtExtracted Texttext/plain53582http://www.lume.ufrgs.br/bitstream/10183/225321/2/000876721.pdf.txt114351a79b1a2f2bb714bbdec5ee074cMD52ORIGINAL000876721.pdfTexto completo (inglês)application/pdf568844http://www.lume.ufrgs.br/bitstream/10183/225321/1/000876721.pdfe273c37c3828ba2a6cf89585b4bb871aMD5110183/2253212023-01-20 06:00:49.480681oai:www.lume.ufrgs.br:10183/225321Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-01-20T08:00:49Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Seizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterization |
title |
Seizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterization |
spellingShingle |
Seizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterization Mussulini, Ben Hur Marins Convulsões Pentilenotetrazol Peixe-zebra |
title_short |
Seizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterization |
title_full |
Seizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterization |
title_fullStr |
Seizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterization |
title_full_unstemmed |
Seizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterization |
title_sort |
Seizures induced by pentylenetetrazole in the adult zebrafish : a detailed behavioral characterization |
author |
Mussulini, Ben Hur Marins |
author_facet |
Mussulini, Ben Hur Marins Leite, Carlos Eduardo Zenki, Kamila Gagliari Moro, Luana Baggio, Suelen Rico, Eduardo Pacheco Rosemberg, Denis Broock Dias, Renato Dutra Souza, Tadeu Mello e Calcagnotto, Maria Elisa Campos, Maria Martha Battastini, Ana Maria Oliveira Oliveira, Diogo Losch de |
author_role |
author |
author2 |
Leite, Carlos Eduardo Zenki, Kamila Gagliari Moro, Luana Baggio, Suelen Rico, Eduardo Pacheco Rosemberg, Denis Broock Dias, Renato Dutra Souza, Tadeu Mello e Calcagnotto, Maria Elisa Campos, Maria Martha Battastini, Ana Maria Oliveira Oliveira, Diogo Losch de |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Mussulini, Ben Hur Marins Leite, Carlos Eduardo Zenki, Kamila Gagliari Moro, Luana Baggio, Suelen Rico, Eduardo Pacheco Rosemberg, Denis Broock Dias, Renato Dutra Souza, Tadeu Mello e Calcagnotto, Maria Elisa Campos, Maria Martha Battastini, Ana Maria Oliveira Oliveira, Diogo Losch de |
dc.subject.por.fl_str_mv |
Convulsões Pentilenotetrazol Peixe-zebra |
topic |
Convulsões Pentilenotetrazol Peixe-zebra |
description |
Pentylenetetrazole (PTZ) is a common convulsant agent used in animal models to investigate the mechanisms of seizures. Although adult zebrafish have been recently used to study epileptic seizures, a thorough characterization of the PTZinduced seizures in this animal model is missing. The goal of this study was to perform a detailed temporal behavior profile characterization of PTZ-induced seizure in adult zebrafish. The behavioral profile during 20 min of PTZ immersion (5, 7.5, 10, and 15 mM) was characterized by stages defined as scores: (0) short swim, (1) increased swimming activity and high frequency of opercular movement, (2) erratic movements, (3) circular movements, (4) clonic seizure-like behavior, (5) fall to the bottom of the tank and tonic seizure-like behavior, (6) death. Animals exposed to distinct PTZ concentrations presented different seizure profiles, intensities and latencies to reach all scores. Only animals immersed into 15 mM PTZ showed an increased time to return to the normal behavior (score 0), after exposure. Total mortality rate at 10 and 15 mM were 33% and 50%, respectively. Considering all behavioral parameters, 5, 7.5, 10, and 15 mM PTZ, induced seizures with low, intermediate, and high severity, respectively. Pretreatment with diazepam (DZP) significantly attenuated seizure severity. Finally, the brain PTZ levels in adult zebrafish immersed into the chemoconvulsant solution at 5 and 10 mM were comparable to those described for the rodent model, with a peak after a 20-min of exposure. The PTZ brain levels observed after 2.5-min PTZ exposure and after 60-min removal from exposure were similar. Altogether, our results showed a detailed temporal behavioral characterization of a PTZ epileptic seizure model in adult zebrafish. These behavioral analyses and the simple method for PTZ quantification could be considered as important tools for future investigations and translational research. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2021-08-06T04:42:49Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/225321 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000876721 |
identifier_str_mv |
1932-6203 000876721 |
url |
http://hdl.handle.net/10183/225321 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PLoS ONE. San Francisco. Vol. 8, no. 1 (Jan. 2013), e54515, 9 f. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/225321/2/000876721.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/225321/1/000876721.pdf |
bitstream.checksum.fl_str_mv |
114351a79b1a2f2bb714bbdec5ee074c e273c37c3828ba2a6cf89585b4bb871a |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801225031510917120 |