Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/178124 |
Resumo: | Background: The relationship between diabetes and oxidative stress has been previously reported. Exercise represents a useful non-pharmacological strategy for the treatment in type 2 diabetic (T2DM) patients, but high intensity exercise can induce a transient inflammatory state and increase oxidative stress. Nutritional strategies that may contribute to the reduction of oxidative stress induced by acute exercise are necessary. The aim of this study was to examine if n-3 PUFA supplementation intervention can attenuate the inflammatory response and oxidative stress associated with high intensity exercise in this population. As a primary outcome, lipoperoxidation measurements (TBARS and F2-isoprostanes) were selected. Methods: Thirty T2DM patients, without chronic complications, were randomly allocated into two groups: placebo (gelatin capsules) or n-3 PUFA (capsules containing 180 mg of eicosapentaenoic acid and 120 mg of docosahexaenoic acid). Blood samples were collected fasting before and after 8 weeks supplementation. In the beginning and at the end of protocol, an acute exercise was performed (treadmill), and new blood samples were collected before and immediately after the exercise for measurements of oxidative stress and high-sensitivity C-reactive protein (hs-CRP). Results: After the supplementation period, a decrease in triglycerides levels was observed only in n-3 PUFA supplementation group (mean difference and 95% CI of 0.002 (0.000–0.004), p = 0.005). Supplementation also significantly reduced TRAP levels after exercise (mean difference and 95% CI to 9641 (− 20,068–39,351) for − 33,884 (− 56,976 - -10,793), p = 0.004, Cohen’s d effect size = 1.12), but no significant difference was observed in n-3 PUFA supplementation group in lipoperoxidation parameters as TBARS (mean difference and 95% CI to − 3.8 (− 10–2.4) for − 2.9 (− 1.6–7.4) or F2-isoprostanes (mean difference and 95% CI -0.05 (− 0.19–0.10) for − 0.02 (− 0.19–0.16), p > 0.05 for both. Conclusion: PUFA n-3 supplementation reduced triglycerides as well as TRAP levels after exercise, without a significant effect on inflammatory and oxidative stress markers. This study is registered at ClinicalTrials.gov with the registration number of NCT03182712. |
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Fayh, Ana Paula TrussardiSapata, Katiuce BorgesCunha, Giovani dos SantosKrause, Maurício da SilvaRocha, RicardoBittencourt Junior, Paulo Ivo Homem deMoreira, Jose Claudio FonsecaFriedman, RogérioRossato, Juliane da SilvaFernandes, João RobertoOliveira, Álvaro Reischak de2018-05-11T02:33:23Z20181550-2783http://hdl.handle.net/10183/178124001066609Background: The relationship between diabetes and oxidative stress has been previously reported. Exercise represents a useful non-pharmacological strategy for the treatment in type 2 diabetic (T2DM) patients, but high intensity exercise can induce a transient inflammatory state and increase oxidative stress. Nutritional strategies that may contribute to the reduction of oxidative stress induced by acute exercise are necessary. The aim of this study was to examine if n-3 PUFA supplementation intervention can attenuate the inflammatory response and oxidative stress associated with high intensity exercise in this population. As a primary outcome, lipoperoxidation measurements (TBARS and F2-isoprostanes) were selected. Methods: Thirty T2DM patients, without chronic complications, were randomly allocated into two groups: placebo (gelatin capsules) or n-3 PUFA (capsules containing 180 mg of eicosapentaenoic acid and 120 mg of docosahexaenoic acid). Blood samples were collected fasting before and after 8 weeks supplementation. In the beginning and at the end of protocol, an acute exercise was performed (treadmill), and new blood samples were collected before and immediately after the exercise for measurements of oxidative stress and high-sensitivity C-reactive protein (hs-CRP). Results: After the supplementation period, a decrease in triglycerides levels was observed only in n-3 PUFA supplementation group (mean difference and 95% CI of 0.002 (0.000–0.004), p = 0.005). Supplementation also significantly reduced TRAP levels after exercise (mean difference and 95% CI to 9641 (− 20,068–39,351) for − 33,884 (− 56,976 - -10,793), p = 0.004, Cohen’s d effect size = 1.12), but no significant difference was observed in n-3 PUFA supplementation group in lipoperoxidation parameters as TBARS (mean difference and 95% CI to − 3.8 (− 10–2.4) for − 2.9 (− 1.6–7.4) or F2-isoprostanes (mean difference and 95% CI -0.05 (− 0.19–0.10) for − 0.02 (− 0.19–0.16), p > 0.05 for both. Conclusion: PUFA n-3 supplementation reduced triglycerides as well as TRAP levels after exercise, without a significant effect on inflammatory and oxidative stress markers. This study is registered at ClinicalTrials.gov with the registration number of NCT03182712.application/pdfengJournal of the international society of sports nutrition. Woodland Park. Vol. 15 (Apr. 2018), 18, [9 p.]Ácidos graxos ômega-3Estresse oxidativoInflamaçãoExercícioDiabetes mellitus tipo 2Omega-3Type 2 diabetesAcute exerciseOxidative stressInflammationEffects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trialEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001066609.pdf001066609.pdfTexto completo (inglês)application/pdf820087http://www.lume.ufrgs.br/bitstream/10183/178124/1/001066609.pdff1109d1c2c209b60cb261d6404411443MD51TEXT001066609.pdf.txt001066609.pdf.txtExtracted Texttext/plain44478http://www.lume.ufrgs.br/bitstream/10183/178124/2/001066609.pdf.txt76e0f1ac31452aeb3008b07cea6a106eMD5210183/1781242019-01-19 02:33:35.380095oai:www.lume.ufrgs.br:10183/178124Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-01-19T04:33:35Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial |
title |
Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial |
spellingShingle |
Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial Fayh, Ana Paula Trussardi Ácidos graxos ômega-3 Estresse oxidativo Inflamação Exercício Diabetes mellitus tipo 2 Omega-3 Type 2 diabetes Acute exercise Oxidative stress Inflammation |
title_short |
Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial |
title_full |
Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial |
title_fullStr |
Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial |
title_full_unstemmed |
Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial |
title_sort |
Effects of n-3 fatty acids and exercise on oxidative stress parameters in type 2 diabetic : a randomized clinical trial |
author |
Fayh, Ana Paula Trussardi |
author_facet |
Fayh, Ana Paula Trussardi Sapata, Katiuce Borges Cunha, Giovani dos Santos Krause, Maurício da Silva Rocha, Ricardo Bittencourt Junior, Paulo Ivo Homem de Moreira, Jose Claudio Fonseca Friedman, Rogério Rossato, Juliane da Silva Fernandes, João Roberto Oliveira, Álvaro Reischak de |
author_role |
author |
author2 |
Sapata, Katiuce Borges Cunha, Giovani dos Santos Krause, Maurício da Silva Rocha, Ricardo Bittencourt Junior, Paulo Ivo Homem de Moreira, Jose Claudio Fonseca Friedman, Rogério Rossato, Juliane da Silva Fernandes, João Roberto Oliveira, Álvaro Reischak de |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Fayh, Ana Paula Trussardi Sapata, Katiuce Borges Cunha, Giovani dos Santos Krause, Maurício da Silva Rocha, Ricardo Bittencourt Junior, Paulo Ivo Homem de Moreira, Jose Claudio Fonseca Friedman, Rogério Rossato, Juliane da Silva Fernandes, João Roberto Oliveira, Álvaro Reischak de |
dc.subject.por.fl_str_mv |
Ácidos graxos ômega-3 Estresse oxidativo Inflamação Exercício Diabetes mellitus tipo 2 |
topic |
Ácidos graxos ômega-3 Estresse oxidativo Inflamação Exercício Diabetes mellitus tipo 2 Omega-3 Type 2 diabetes Acute exercise Oxidative stress Inflammation |
dc.subject.eng.fl_str_mv |
Omega-3 Type 2 diabetes Acute exercise Oxidative stress Inflammation |
description |
Background: The relationship between diabetes and oxidative stress has been previously reported. Exercise represents a useful non-pharmacological strategy for the treatment in type 2 diabetic (T2DM) patients, but high intensity exercise can induce a transient inflammatory state and increase oxidative stress. Nutritional strategies that may contribute to the reduction of oxidative stress induced by acute exercise are necessary. The aim of this study was to examine if n-3 PUFA supplementation intervention can attenuate the inflammatory response and oxidative stress associated with high intensity exercise in this population. As a primary outcome, lipoperoxidation measurements (TBARS and F2-isoprostanes) were selected. Methods: Thirty T2DM patients, without chronic complications, were randomly allocated into two groups: placebo (gelatin capsules) or n-3 PUFA (capsules containing 180 mg of eicosapentaenoic acid and 120 mg of docosahexaenoic acid). Blood samples were collected fasting before and after 8 weeks supplementation. In the beginning and at the end of protocol, an acute exercise was performed (treadmill), and new blood samples were collected before and immediately after the exercise for measurements of oxidative stress and high-sensitivity C-reactive protein (hs-CRP). Results: After the supplementation period, a decrease in triglycerides levels was observed only in n-3 PUFA supplementation group (mean difference and 95% CI of 0.002 (0.000–0.004), p = 0.005). Supplementation also significantly reduced TRAP levels after exercise (mean difference and 95% CI to 9641 (− 20,068–39,351) for − 33,884 (− 56,976 - -10,793), p = 0.004, Cohen’s d effect size = 1.12), but no significant difference was observed in n-3 PUFA supplementation group in lipoperoxidation parameters as TBARS (mean difference and 95% CI to − 3.8 (− 10–2.4) for − 2.9 (− 1.6–7.4) or F2-isoprostanes (mean difference and 95% CI -0.05 (− 0.19–0.10) for − 0.02 (− 0.19–0.16), p > 0.05 for both. Conclusion: PUFA n-3 supplementation reduced triglycerides as well as TRAP levels after exercise, without a significant effect on inflammatory and oxidative stress markers. This study is registered at ClinicalTrials.gov with the registration number of NCT03182712. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-05-11T02:33:23Z |
dc.date.issued.fl_str_mv |
2018 |
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Journal of the international society of sports nutrition. Woodland Park. Vol. 15 (Apr. 2018), 18, [9 p.] |
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