Analysis of the ntpdase and ecto-5´-nucleotidase profiles in serum-limited trichomonas vaginalis

• Autor(a) principal: Frasson, Amanda Piccoli
• Data de Publicação: 2012
• Outros Autores: Charão, Mariele Feiffer, Rosemberg, Denis Broock, Souza, Ana Paula de, Garcia, Solange Cristina, Bonorino, Cristina Beatriz Cazabuena, Bogo, Mauricio Reis, De Carli, Geraldo Attilio, Tasca, Tiana
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/72280
• topic: Trichomonas vaginalis; Ecto-5'-nucleotidase; Adenosina; Trichomonas vaginalis; Serum limitation; Ectonucleoside triphosphate diphosphohydrolase; Ecto-5’; Nucleotidase; Adenosine

Trichomonas vaginalis is a parasite of the human urogenital tract that causes trichomonosis, the most prevalent non-viral sexually transmitted disease. Ectonucleoside triphosphate diphosphohydrolase (NTPDase) family mem- bers, which hydrolyse extracellular ATP and ADP and ecto-5′-nucleotidase, which hydrolyses AMP, have been char- acterised in T. vaginalis. For trichomonad culture, the growth medium is supplemented with 10% serum, which is an important source of nutrients, such as adenosine. Here, we investigated the ATP metabolism of T. vaginalis tropho- zoites from long-term cultures and clinical isolates under limited bovine serum conditions (1% serum). The specific enzymatic activities were expressed as nmol inorganic phosphate (Pi) released/min/mg protein, the gene expression patterns were determined by reverse transcriptase-polymerase chain reaction, the extracellular adenine nucleotide hydrolysis was analysed by high performance liquid chromatography and the cell cycle analysis was assessed by f low cytometry. Serum limitation led to the profound activation of NTPDase and ecto-5’-nucleotidase activities. Furthermore, the levels of NTPDase A and B transcripts increased and extracellular ATP metabolism was activated, which led to enhanced ATP hydrolysis and the formation of ADP and AMP. Moreover, the cell cycle was arrested at the G0/G1 stage, which suggested adenosine uptake. Our data suggest that under conditions of serum limitation, NTPDase and ecto-5’-nucleotidase play a role in providing the adenosine required for T. vaginalis growth and that this process contributes to the establishment of parasitism.