Quantification of mixed chimerism allows early therapeutic interventions

Detalhes bibliográficos
Autor(a) principal: Merzoni, Jóice
Data de Publicação: 2014
Outros Autores: Ewald, Gisele Menezes, Paz, Alessandra Aparecida, Daudt, Liane Esteves, Jobim, Luiz Fernando Job
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/129045
Resumo: Hematopoietic stem cell transplantation is the curative option for patients with myelodys-plastic syndrome; however, it requires a long post-transplantation follow-up. A 53-year-oldwoman with a diagnosis of myelodysplastic syndrome underwent related donor allogeneichematopoietic stem cell transplantation in July 2006. Three months after transplanta-tion, a comparative short tandem repeat analysis between donor and recipient revealedfull chimerism, indicating complete, healthy bone marrow reconstitution. Three yearsand ten months after hematopoietic stem cell transplantation, the patient developedleukopenia and thrombocytopenia. Another short tandem repeat analysis was carried outwhich showed mixed chimerism (52.62%), indicating relapsed disease. A donor lymphocyteinfusion was administered. The purpose of donor lymphocyte infusion is to induce a graft-versus-leukemia effect; in fact, this donor’s lymphocyte infusion induced full chimerism.Successive short tandem repeat analyses were performed as part of post-transplantationfollow-up, and in July 2010, one such analysis again showed mixed chimerism (64.25%).Based on this finding, a second donor lymphocyte infusion was administered, but failedto eradicate the disease. In September 2011, the patient presented with relapsed dis-ease, and a second related donor allogeneic hematopoietic stem cell transplantationwas performed. Subsequent short tandem repeat analyses revealed full chimerism, indi-cating complete bone marrow reconstitution. We conclude that quantitative detectionof mixed chimerism is an important diagnostic tool that can guide early therapeuticintervention.
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spelling Merzoni, JóiceEwald, Gisele MenezesPaz, Alessandra AparecidaDaudt, Liane EstevesJobim, Luiz Fernando Job2015-11-07T02:37:10Z20141516-8484http://hdl.handle.net/10183/129045000972938Hematopoietic stem cell transplantation is the curative option for patients with myelodys-plastic syndrome; however, it requires a long post-transplantation follow-up. A 53-year-oldwoman with a diagnosis of myelodysplastic syndrome underwent related donor allogeneichematopoietic stem cell transplantation in July 2006. Three months after transplanta-tion, a comparative short tandem repeat analysis between donor and recipient revealedfull chimerism, indicating complete, healthy bone marrow reconstitution. Three yearsand ten months after hematopoietic stem cell transplantation, the patient developedleukopenia and thrombocytopenia. Another short tandem repeat analysis was carried outwhich showed mixed chimerism (52.62%), indicating relapsed disease. A donor lymphocyteinfusion was administered. The purpose of donor lymphocyte infusion is to induce a graft-versus-leukemia effect; in fact, this donor’s lymphocyte infusion induced full chimerism.Successive short tandem repeat analyses were performed as part of post-transplantationfollow-up, and in July 2010, one such analysis again showed mixed chimerism (64.25%).Based on this finding, a second donor lymphocyte infusion was administered, but failedto eradicate the disease. In September 2011, the patient presented with relapsed dis-ease, and a second related donor allogeneic hematopoietic stem cell transplantationwas performed. Subsequent short tandem repeat analyses revealed full chimerism, indi-cating complete bone marrow reconstitution. We conclude that quantitative detectionof mixed chimerism is an important diagnostic tool that can guide early therapeuticintervention.application/pdfporRevista brasileira de hematologia e hemoterapia. São Paulo. Vol. 36, n. 5 (set./out. 2014), p. 369-372QuimerismoTransplante de medula ósseaDoenças mieloproliferativas-mielodisplásicasSequências repetidas em TandemChimerismBone marrow transplantationMyelodysplastic myeloproliferativediseasesTandem repeat sequencesaQuantification of mixed chimerism allows early therapeutic interventionsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000972938.pdf000972938.pdfTexto completo (inglês)application/pdf354057http://www.lume.ufrgs.br/bitstream/10183/129045/1/000972938.pdfa1e360d430e78d82fdff3e35d887301eMD51TEXT000972938.pdf.txt000972938.pdf.txtExtracted Texttext/plain15200http://www.lume.ufrgs.br/bitstream/10183/129045/2/000972938.pdf.txtc2c24782f00cafc26b8dec1e419a643eMD52THUMBNAIL000972938.pdf.jpg000972938.pdf.jpgGenerated Thumbnailimage/jpeg1908http://www.lume.ufrgs.br/bitstream/10183/129045/3/000972938.pdf.jpgef6b05755b0e129ff72a800e342595f1MD5310183/1290452018-10-24 09:09:18.73oai:www.lume.ufrgs.br:10183/129045Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-24T12:09:18Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Quantification of mixed chimerism allows early therapeutic interventions
title Quantification of mixed chimerism allows early therapeutic interventions
spellingShingle Quantification of mixed chimerism allows early therapeutic interventions
Merzoni, Jóice
Quimerismo
Transplante de medula óssea
Doenças mieloproliferativas-mielodisplásicas
Sequências repetidas em Tandem
Chimerism
Bone marrow transplantation
Myelodysplastic myeloproliferativediseases
Tandem repeat sequencesa
title_short Quantification of mixed chimerism allows early therapeutic interventions
title_full Quantification of mixed chimerism allows early therapeutic interventions
title_fullStr Quantification of mixed chimerism allows early therapeutic interventions
title_full_unstemmed Quantification of mixed chimerism allows early therapeutic interventions
title_sort Quantification of mixed chimerism allows early therapeutic interventions
author Merzoni, Jóice
author_facet Merzoni, Jóice
Ewald, Gisele Menezes
Paz, Alessandra Aparecida
Daudt, Liane Esteves
Jobim, Luiz Fernando Job
author_role author
author2 Ewald, Gisele Menezes
Paz, Alessandra Aparecida
Daudt, Liane Esteves
Jobim, Luiz Fernando Job
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Merzoni, Jóice
Ewald, Gisele Menezes
Paz, Alessandra Aparecida
Daudt, Liane Esteves
Jobim, Luiz Fernando Job
dc.subject.por.fl_str_mv Quimerismo
Transplante de medula óssea
Doenças mieloproliferativas-mielodisplásicas
Sequências repetidas em Tandem
topic Quimerismo
Transplante de medula óssea
Doenças mieloproliferativas-mielodisplásicas
Sequências repetidas em Tandem
Chimerism
Bone marrow transplantation
Myelodysplastic myeloproliferativediseases
Tandem repeat sequencesa
dc.subject.eng.fl_str_mv Chimerism
Bone marrow transplantation
Myelodysplastic myeloproliferativediseases
Tandem repeat sequencesa
description Hematopoietic stem cell transplantation is the curative option for patients with myelodys-plastic syndrome; however, it requires a long post-transplantation follow-up. A 53-year-oldwoman with a diagnosis of myelodysplastic syndrome underwent related donor allogeneichematopoietic stem cell transplantation in July 2006. Three months after transplanta-tion, a comparative short tandem repeat analysis between donor and recipient revealedfull chimerism, indicating complete, healthy bone marrow reconstitution. Three yearsand ten months after hematopoietic stem cell transplantation, the patient developedleukopenia and thrombocytopenia. Another short tandem repeat analysis was carried outwhich showed mixed chimerism (52.62%), indicating relapsed disease. A donor lymphocyteinfusion was administered. The purpose of donor lymphocyte infusion is to induce a graft-versus-leukemia effect; in fact, this donor’s lymphocyte infusion induced full chimerism.Successive short tandem repeat analyses were performed as part of post-transplantationfollow-up, and in July 2010, one such analysis again showed mixed chimerism (64.25%).Based on this finding, a second donor lymphocyte infusion was administered, but failedto eradicate the disease. In September 2011, the patient presented with relapsed dis-ease, and a second related donor allogeneic hematopoietic stem cell transplantationwas performed. Subsequent short tandem repeat analyses revealed full chimerism, indi-cating complete bone marrow reconstitution. We conclude that quantitative detectionof mixed chimerism is an important diagnostic tool that can guide early therapeuticintervention.
publishDate 2014
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dc.relation.ispartof.pt_BR.fl_str_mv Revista brasileira de hematologia e hemoterapia. São Paulo. Vol. 36, n. 5 (set./out. 2014), p. 369-372
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