Effect of the systemic administration of methylprednisolone on the lungs of brain-dead donor rats undergoing pulmonary transplantation

• Autor(a) principal: Araujo, Luiz Felipe Lopes
• Data de Publicação: 2013
• Outros Autores: Holand, Arthur Rodrigo Ronconi, Paludo, Artur de Oliveira, Silva, Everton Franco, Forgiarini Júnior, Luiz Alberto, Forgiarini, Luiz Felipe, Silva, Mariel Barbachan e, Andrade, Cristiano Feijó
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/194167
• topic: Anti-inflamatórios; Morte encefálica; Transplante de pulmão; Metilprednisolona; Estresse oxidativo; Modelos animais de doenças; Lung transplantation; Oxidative stress; Methylprednisolone; Experimental model; Brain death

OBJECTIVE: Most lung transplants are obtained from brain-dead donors. The physiopathology of brain death involves hemodynamics, the sympathetic nervous system, and inflammatory mechanisms. Administering methylprednisolone 60 min after inducing brain death in rats has been shown to modulate pulmonary inflammatory activity. Our objective was to evaluate the effects of methylprednisolone on transplanted rat lungs from donors treated 60 min after brain death. METHODS: Twelve Wistar rats were anesthetized, and brain death was induced. They were randomly divided into two groups (n = 6), namely a control group, which was administered saline solution, and a methylprednisolone group, which received the drug 60 min after the induction of brain death. All of the animals were observed and ventilated for 2 h prior to being submitted to lung transplantation. We evaluated the hemodynamic and blood gas parameters, histological score, lung tissue levels of thiobarbituric acidreactive substances, level of superoxide dismutase, level of tumor necrosis factor-alpha, and level of interleukin- 1 beta. RESULTS: After transplantation, a significant reduction in the levels of tumor necrosis factor-alpha and IL-1b was observed in the group that received methylprednisolone (p=0.0084 and p=0.0155, respectively). There were no significant differences in tumor necrosis factor-alpha and superoxide dismutase levels between the control and methylprednisolone groups (p=0.2644 and p=0.7461, respectively). There were no significant differences in the blood gas parameters, hemodynamics, and histological alterations between the groups. CONCLUSION: The administration of methylprednisolone after brain death in donor rats reduces inflammatory activity in transplanted lungs but has no influence on parameters related to oxidative stress.