Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure

Detalhes bibliográficos
Autor(a) principal: Sehnem, Nicole Teixeira
Data de Publicação: 2020
Outros Autores: Machado, Angela da Silva, Matte, Carla Roberta, Morais Junior, Marcos Antonio de, Ayub, Marco Antônio Záchia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/263629
Resumo: The aims of this work were to improve cell tolerance towards high concentrations of furfural and 5-hydroxymethylfurfural (HMF) of an osmotolerant strain of Wickerhamomyces anomalus by means of evolutionary engineering, and to determine its ethanol production under stress conditions. Cells were grown in the presence of furfural, HMF, either isolated or in combination, and under high osmotic pressure conditions. The most toxic condition for the parental strain was the combination of both furans, under which it was unable to grow and to produce ethanol. However, the tolerant adapted strain achieved a yield of ethanol of 0.43 g g-1glucose in the presence of furfural and HMF, showing an alcohol dehydrogenase activity of 0.68 mU mg protein-1. For this strain, osmotic pressure, did not affect its growth rate. These results suggest that W. anomalus WA-HF5.5strain shows potential to be used in second-generation ethanol production systems.
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spelling Sehnem, Nicole TeixeiraMachado, Angela da SilvaMatte, Carla RobertaMorais Junior, Marcos Antonio deAyub, Marco Antônio Záchia2023-08-16T03:33:15Z20200001-3765http://hdl.handle.net/10183/263629001167931The aims of this work were to improve cell tolerance towards high concentrations of furfural and 5-hydroxymethylfurfural (HMF) of an osmotolerant strain of Wickerhamomyces anomalus by means of evolutionary engineering, and to determine its ethanol production under stress conditions. Cells were grown in the presence of furfural, HMF, either isolated or in combination, and under high osmotic pressure conditions. The most toxic condition for the parental strain was the combination of both furans, under which it was unable to grow and to produce ethanol. However, the tolerant adapted strain achieved a yield of ethanol of 0.43 g g-1glucose in the presence of furfural and HMF, showing an alcohol dehydrogenase activity of 0.68 mU mg protein-1. For this strain, osmotic pressure, did not affect its growth rate. These results suggest that W. anomalus WA-HF5.5strain shows potential to be used in second-generation ethanol production systems.application/pdfengAnais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 92, suppl. 2 (2020), e20181030, 12 p.EtanolPressão osmóticaFuraldeídoSecond-generation ethanolWickerhamomyces anomalusFuraldehydes toleranceOsmotic pressureLignocellulosic hydrolysatesSecond-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressureinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001167931.pdf.txt001167931.pdf.txtExtracted Texttext/plain38053http://www.lume.ufrgs.br/bitstream/10183/263629/2/001167931.pdf.txt47b24ac7173d5b20b6817df1ff3c3214MD52ORIGINAL001167931.pdfTexto completoapplication/pdf962774http://www.lume.ufrgs.br/bitstream/10183/263629/1/001167931.pdfeb23743218313087b13f464c48a82dc6MD5110183/2636292023-08-17 03:36:10.364758oai:www.lume.ufrgs.br:10183/263629Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-17T06:36:10Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure
title Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure
spellingShingle Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure
Sehnem, Nicole Teixeira
Etanol
Pressão osmótica
Furaldeído
Second-generation ethanol
Wickerhamomyces anomalus
Furaldehydes tolerance
Osmotic pressure
Lignocellulosic hydrolysates
title_short Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure
title_full Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure
title_fullStr Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure
title_full_unstemmed Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure
title_sort Second-generation ethanol production by Wickerhamomyces anomalus strain adapted to furfural, 5-hydroxymethylfurfural (HMF), and high osmotic pressure
author Sehnem, Nicole Teixeira
author_facet Sehnem, Nicole Teixeira
Machado, Angela da Silva
Matte, Carla Roberta
Morais Junior, Marcos Antonio de
Ayub, Marco Antônio Záchia
author_role author
author2 Machado, Angela da Silva
Matte, Carla Roberta
Morais Junior, Marcos Antonio de
Ayub, Marco Antônio Záchia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Sehnem, Nicole Teixeira
Machado, Angela da Silva
Matte, Carla Roberta
Morais Junior, Marcos Antonio de
Ayub, Marco Antônio Záchia
dc.subject.por.fl_str_mv Etanol
Pressão osmótica
Furaldeído
topic Etanol
Pressão osmótica
Furaldeído
Second-generation ethanol
Wickerhamomyces anomalus
Furaldehydes tolerance
Osmotic pressure
Lignocellulosic hydrolysates
dc.subject.eng.fl_str_mv Second-generation ethanol
Wickerhamomyces anomalus
Furaldehydes tolerance
Osmotic pressure
Lignocellulosic hydrolysates
description The aims of this work were to improve cell tolerance towards high concentrations of furfural and 5-hydroxymethylfurfural (HMF) of an osmotolerant strain of Wickerhamomyces anomalus by means of evolutionary engineering, and to determine its ethanol production under stress conditions. Cells were grown in the presence of furfural, HMF, either isolated or in combination, and under high osmotic pressure conditions. The most toxic condition for the parental strain was the combination of both furans, under which it was unable to grow and to produce ethanol. However, the tolerant adapted strain achieved a yield of ethanol of 0.43 g g-1glucose in the presence of furfural and HMF, showing an alcohol dehydrogenase activity of 0.68 mU mg protein-1. For this strain, osmotic pressure, did not affect its growth rate. These results suggest that W. anomalus WA-HF5.5strain shows potential to be used in second-generation ethanol production systems.
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2023-08-16T03:33:15Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/263629
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dc.identifier.nrb.pt_BR.fl_str_mv 001167931
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Anais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 92, suppl. 2 (2020), e20181030, 12 p.
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dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
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institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
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