Characterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sediment
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/262350 |
Resumo: | Bacteria of the genus Bacillus sp. present the potential for inhibiting various pathogens, making them a promising starting point in the search for new antimicrobial substances. In this study, bacteria were isolated from sediment samples from humid areas of a Natural Conservation Unit in the state of Rio Grande do Sul, Brazil. The isolate Bacillus sp. sed 1.4 was selected for production of antimicrobial activity, and was characterized by MALDI-TOF and 16S rDNA sequencing. Phylogenetic analysis showed that Bacillus sed 1.4 was closely related to Bacillus altitudinis and Bacillus pumilus. The cell-free supernatant was partially purifi ed using ammonium sulfate precipitation, gel fi ltration chromatography (Sephadex G-200) and an ultrafi ltration membrane. Partial purifi cation resulted in specifi c activity of 769.23 AU/mg, with a molecular mass of approximately 148 kDa. This antimicrobial substance showed stability at 100°C for 5 min, and was inactivated by proteolytic enzymes. An antimicrobial effect against Listeria species was observed. Considering the importance of the Listeria genus in the area of food safety, this antimicrobial activity should be further explored, specifi cally in the fi eld of dairy products and with a focus on food biopreservation studies. |
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Cavalini, LucianiJankoski, Priscila RibeiroCorrea, Ana Paula FolmerBrandelli, AdrianoMotta, Amanda de Souza da2023-07-19T03:39:15Z20210001-3765http://hdl.handle.net/10183/262350001168307Bacteria of the genus Bacillus sp. present the potential for inhibiting various pathogens, making them a promising starting point in the search for new antimicrobial substances. In this study, bacteria were isolated from sediment samples from humid areas of a Natural Conservation Unit in the state of Rio Grande do Sul, Brazil. The isolate Bacillus sp. sed 1.4 was selected for production of antimicrobial activity, and was characterized by MALDI-TOF and 16S rDNA sequencing. Phylogenetic analysis showed that Bacillus sed 1.4 was closely related to Bacillus altitudinis and Bacillus pumilus. The cell-free supernatant was partially purifi ed using ammonium sulfate precipitation, gel fi ltration chromatography (Sephadex G-200) and an ultrafi ltration membrane. Partial purifi cation resulted in specifi c activity of 769.23 AU/mg, with a molecular mass of approximately 148 kDa. This antimicrobial substance showed stability at 100°C for 5 min, and was inactivated by proteolytic enzymes. An antimicrobial effect against Listeria species was observed. Considering the importance of the Listeria genus in the area of food safety, this antimicrobial activity should be further explored, specifi cally in the fi eld of dairy products and with a focus on food biopreservation studies.application/pdfengAnais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 93, supp. 4 (2021), e20201820, 13 p.Conservação de alimentosSubstância bioativaAtividade antimicrobianaFilogenéticaFood conservationListeria sp.Wetland sedimentsBioactive substancesCharacterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sedimentinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001168307.pdf.txt001168307.pdf.txtExtracted Texttext/plain42447http://www.lume.ufrgs.br/bitstream/10183/262350/2/001168307.pdf.txtffaae99e09c33ba0042ed312c61d5209MD52ORIGINAL001168307.pdfTexto completo (inglês)application/pdf1008962http://www.lume.ufrgs.br/bitstream/10183/262350/1/001168307.pdf35d2df7aeb716d2efc5361db7639cebbMD5110183/2623502023-07-20 03:35:35.193601oai:www.lume.ufrgs.br:10183/262350Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-07-20T06:35:35Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Characterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sediment |
title |
Characterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sediment |
spellingShingle |
Characterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sediment Cavalini, Luciani Conservação de alimentos Substância bioativa Atividade antimicrobiana Filogenética Food conservation Listeria sp. Wetland sediments Bioactive substances |
title_short |
Characterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sediment |
title_full |
Characterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sediment |
title_fullStr |
Characterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sediment |
title_full_unstemmed |
Characterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sediment |
title_sort |
Characterization of the antimicrobial activity produced by Bacillus sp. isolated from wetland sediment |
author |
Cavalini, Luciani |
author_facet |
Cavalini, Luciani Jankoski, Priscila Ribeiro Correa, Ana Paula Folmer Brandelli, Adriano Motta, Amanda de Souza da |
author_role |
author |
author2 |
Jankoski, Priscila Ribeiro Correa, Ana Paula Folmer Brandelli, Adriano Motta, Amanda de Souza da |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cavalini, Luciani Jankoski, Priscila Ribeiro Correa, Ana Paula Folmer Brandelli, Adriano Motta, Amanda de Souza da |
dc.subject.por.fl_str_mv |
Conservação de alimentos Substância bioativa Atividade antimicrobiana Filogenética |
topic |
Conservação de alimentos Substância bioativa Atividade antimicrobiana Filogenética Food conservation Listeria sp. Wetland sediments Bioactive substances |
dc.subject.eng.fl_str_mv |
Food conservation Listeria sp. Wetland sediments Bioactive substances |
description |
Bacteria of the genus Bacillus sp. present the potential for inhibiting various pathogens, making them a promising starting point in the search for new antimicrobial substances. In this study, bacteria were isolated from sediment samples from humid areas of a Natural Conservation Unit in the state of Rio Grande do Sul, Brazil. The isolate Bacillus sp. sed 1.4 was selected for production of antimicrobial activity, and was characterized by MALDI-TOF and 16S rDNA sequencing. Phylogenetic analysis showed that Bacillus sed 1.4 was closely related to Bacillus altitudinis and Bacillus pumilus. The cell-free supernatant was partially purifi ed using ammonium sulfate precipitation, gel fi ltration chromatography (Sephadex G-200) and an ultrafi ltration membrane. Partial purifi cation resulted in specifi c activity of 769.23 AU/mg, with a molecular mass of approximately 148 kDa. This antimicrobial substance showed stability at 100°C for 5 min, and was inactivated by proteolytic enzymes. An antimicrobial effect against Listeria species was observed. Considering the importance of the Listeria genus in the area of food safety, this antimicrobial activity should be further explored, specifi cally in the fi eld of dairy products and with a focus on food biopreservation studies. |
publishDate |
2021 |
dc.date.issued.fl_str_mv |
2021 |
dc.date.accessioned.fl_str_mv |
2023-07-19T03:39:15Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
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info:eu-repo/semantics/publishedVersion |
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article |
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http://hdl.handle.net/10183/262350 |
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0001-3765 |
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001168307 |
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http://hdl.handle.net/10183/262350 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Anais da Academia Brasileira de Ciências. Rio de Janeiro. Vol. 93, supp. 4 (2021), e20201820, 13 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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