A proteomic insight into vitellogenesis during tick ovary maturation
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/175011 |
Resumo: | Ticks are arthropod ectoparasites of importance for public and veterinary health. The understanding of tick oogenesis and embryogenesis could contribute to the development of novel control methods. However, to date, studies on the temporal dynamics of proteins during ovary development were not reported. In the present study we followed protein profile during ovary maturation. Proteomic analysis of ovary extracts was performed by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) using shotgun strategy, in addition to dimethyl labelling-based protein quantification. A total of 3,756 proteins were identified, which were functionally annotated into 30 categories. Circa 80% of the annotated proteins belong to categories related to basal metabolism, such as protein synthesis and modification machineries, nuclear regulation, cytoskeleton, proteasome machinery, transcriptional machinery, energetic metabolism, extracellular matrix/cell adhesion, immunity, oxidation/ detoxification metabolism, signal transduction, and storage. The abundance of selected proteins involved in yolk uptake and degradation, as well as vitellin accumulation during ovary maturation, was assessed using dimethyl-labelling quantification. In conclusion, proteins identified in this study provide a framework for future studies to elucidate tick development and validate candidate targets for novel control methods. |
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Xavier, Marina AmaralTirloni, LucasPinto, Antonio Frederico MichelDiedrich, Jolene K.Yates III, John R.Mulenga, AlbertLogullo, CarlosVaz Junior, Itabajara da SilvaSeixas, AdrianaTermignoni, Carlos2018-04-26T02:32:38Z20182045-2322http://hdl.handle.net/10183/175011001064303Ticks are arthropod ectoparasites of importance for public and veterinary health. The understanding of tick oogenesis and embryogenesis could contribute to the development of novel control methods. However, to date, studies on the temporal dynamics of proteins during ovary development were not reported. In the present study we followed protein profile during ovary maturation. Proteomic analysis of ovary extracts was performed by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) using shotgun strategy, in addition to dimethyl labelling-based protein quantification. A total of 3,756 proteins were identified, which were functionally annotated into 30 categories. Circa 80% of the annotated proteins belong to categories related to basal metabolism, such as protein synthesis and modification machineries, nuclear regulation, cytoskeleton, proteasome machinery, transcriptional machinery, energetic metabolism, extracellular matrix/cell adhesion, immunity, oxidation/ detoxification metabolism, signal transduction, and storage. The abundance of selected proteins involved in yolk uptake and degradation, as well as vitellin accumulation during ovary maturation, was assessed using dimethyl-labelling quantification. In conclusion, proteins identified in this study provide a framework for future studies to elucidate tick development and validate candidate targets for novel control methods.application/pdfengScientific reports. London. Vol. 8 (2018), 4698, 14 p.Rhipicephalus microplusOogeneseVitelogêneseEmbriogeneseProteômicaControle biológicoProteome informaticsProteomicsA proteomic insight into vitellogenesis during tick ovary maturationEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001064303.pdf001064303.pdfTexto completo (inglês)application/pdf3571881http://www.lume.ufrgs.br/bitstream/10183/175011/1/001064303.pdf67eb1ad13735749f2ae5db688ecb012aMD51TEXT001064303.pdf.txt001064303.pdf.txtExtracted Texttext/plain64650http://www.lume.ufrgs.br/bitstream/10183/175011/2/001064303.pdf.txtc910330482cab48d4c184632252237d7MD52THUMBNAIL001064303.pdf.jpg001064303.pdf.jpgGenerated Thumbnailimage/jpeg2049http://www.lume.ufrgs.br/bitstream/10183/175011/3/001064303.pdf.jpgcf7f956197ae891bc47086e077ec3ec0MD5310183/1750112024-05-01 06:51:00.936286oai:www.lume.ufrgs.br:10183/175011Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-05-01T09:51Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
A proteomic insight into vitellogenesis during tick ovary maturation |
title |
A proteomic insight into vitellogenesis during tick ovary maturation |
spellingShingle |
A proteomic insight into vitellogenesis during tick ovary maturation Xavier, Marina Amaral Rhipicephalus microplus Oogenese Vitelogênese Embriogenese Proteômica Controle biológico Proteome informatics Proteomics |
title_short |
A proteomic insight into vitellogenesis during tick ovary maturation |
title_full |
A proteomic insight into vitellogenesis during tick ovary maturation |
title_fullStr |
A proteomic insight into vitellogenesis during tick ovary maturation |
title_full_unstemmed |
A proteomic insight into vitellogenesis during tick ovary maturation |
title_sort |
A proteomic insight into vitellogenesis during tick ovary maturation |
author |
Xavier, Marina Amaral |
author_facet |
Xavier, Marina Amaral Tirloni, Lucas Pinto, Antonio Frederico Michel Diedrich, Jolene K. Yates III, John R. Mulenga, Albert Logullo, Carlos Vaz Junior, Itabajara da Silva Seixas, Adriana Termignoni, Carlos |
author_role |
author |
author2 |
Tirloni, Lucas Pinto, Antonio Frederico Michel Diedrich, Jolene K. Yates III, John R. Mulenga, Albert Logullo, Carlos Vaz Junior, Itabajara da Silva Seixas, Adriana Termignoni, Carlos |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Xavier, Marina Amaral Tirloni, Lucas Pinto, Antonio Frederico Michel Diedrich, Jolene K. Yates III, John R. Mulenga, Albert Logullo, Carlos Vaz Junior, Itabajara da Silva Seixas, Adriana Termignoni, Carlos |
dc.subject.por.fl_str_mv |
Rhipicephalus microplus Oogenese Vitelogênese Embriogenese Proteômica Controle biológico |
topic |
Rhipicephalus microplus Oogenese Vitelogênese Embriogenese Proteômica Controle biológico Proteome informatics Proteomics |
dc.subject.eng.fl_str_mv |
Proteome informatics Proteomics |
description |
Ticks are arthropod ectoparasites of importance for public and veterinary health. The understanding of tick oogenesis and embryogenesis could contribute to the development of novel control methods. However, to date, studies on the temporal dynamics of proteins during ovary development were not reported. In the present study we followed protein profile during ovary maturation. Proteomic analysis of ovary extracts was performed by liquid chromatography-tandem mass spectrometry (LC-MS/ MS) using shotgun strategy, in addition to dimethyl labelling-based protein quantification. A total of 3,756 proteins were identified, which were functionally annotated into 30 categories. Circa 80% of the annotated proteins belong to categories related to basal metabolism, such as protein synthesis and modification machineries, nuclear regulation, cytoskeleton, proteasome machinery, transcriptional machinery, energetic metabolism, extracellular matrix/cell adhesion, immunity, oxidation/ detoxification metabolism, signal transduction, and storage. The abundance of selected proteins involved in yolk uptake and degradation, as well as vitellin accumulation during ovary maturation, was assessed using dimethyl-labelling quantification. In conclusion, proteins identified in this study provide a framework for future studies to elucidate tick development and validate candidate targets for novel control methods. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-04-26T02:32:38Z |
dc.date.issued.fl_str_mv |
2018 |
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Estrangeiro info:eu-repo/semantics/article |
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2045-2322 |
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001064303 |
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eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Scientific reports. London. Vol. 8 (2018), 4698, 14 p. |
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openAccess |
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