Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/111824 |
Resumo: | Background: The pathophysiology of recurrent pregnancy loss (RPL) is still unknown in 50% of the cases. Herein we measure the expression of beta3 integrin subunit, a well-known implantation marker, in women with or without RPL and correlate it with the histological dating of the endometrial tissue. Methods: LH-timed endometrial biopsies were obtained from cases (RPL; n = 21, age 33.9+/−4.7) and healthy controls (n = 29; age 29.8+/−4.1) during the mid-secretory phase (post ovulatory day: 8 to 10). Endometrial samples were timed histologically according to Noyes’ criteria and underwent immunohistochemical staining for beta3 integrin expression. For statistical analysis the semi-quantitative HSCORE was assessed. Type I (beta3 negative in an out-of-phase endometrium) and Type II defect (beta3 negative in an in-phase endometrium) were also analysed. Statistical analysis was done with Student t-test, Mann Whitney U test, ANCOVA and chi square for trend. Significance was set as P < 0.05. Results: The mean (SD) age in controls was lower compared to cases [(29.8 (4.1) vs. 33.9 (4.7) – P = 0.001; Student t-test)]. The median (range) expression of beta3 integrin in controls and cases was 1.94 (0 to 3.5) vs. 0.82 (0 to 3.6), respectively (P = 0.001; Mann Whitney U test). Significance was still significant after adjusting for age (P = 0.03;ANCOVA). The normal positive staining > =0.7 of beta3 integrin subunit and in-phase endometrium was seen in 24 out of 29 (82.8%) controls, but in only 6 out of 21 (28.6%) of cases with RPL; Type I and II defects were seen in 10.3 and 6.9% of controls, while present in 52.4 and 19.1% of cases, respectively (P = 0.0005; chi-square). Conclusions: Women with unexplained RPL had significantly reduced integrin expression compared to controls. Our findings underline the need for further molecular analysis of endometrial tissue in affected women. |
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Germeyer, ArianeSavaris, Ricardo FrancalacciJauckus, JuliaLessey, Bruce Arthur2015-03-07T01:57:09Z20141477-7827http://hdl.handle.net/10183/111824000953079Background: The pathophysiology of recurrent pregnancy loss (RPL) is still unknown in 50% of the cases. Herein we measure the expression of beta3 integrin subunit, a well-known implantation marker, in women with or without RPL and correlate it with the histological dating of the endometrial tissue. Methods: LH-timed endometrial biopsies were obtained from cases (RPL; n = 21, age 33.9+/−4.7) and healthy controls (n = 29; age 29.8+/−4.1) during the mid-secretory phase (post ovulatory day: 8 to 10). Endometrial samples were timed histologically according to Noyes’ criteria and underwent immunohistochemical staining for beta3 integrin expression. For statistical analysis the semi-quantitative HSCORE was assessed. Type I (beta3 negative in an out-of-phase endometrium) and Type II defect (beta3 negative in an in-phase endometrium) were also analysed. Statistical analysis was done with Student t-test, Mann Whitney U test, ANCOVA and chi square for trend. Significance was set as P < 0.05. Results: The mean (SD) age in controls was lower compared to cases [(29.8 (4.1) vs. 33.9 (4.7) – P = 0.001; Student t-test)]. The median (range) expression of beta3 integrin in controls and cases was 1.94 (0 to 3.5) vs. 0.82 (0 to 3.6), respectively (P = 0.001; Mann Whitney U test). Significance was still significant after adjusting for age (P = 0.03;ANCOVA). The normal positive staining > =0.7 of beta3 integrin subunit and in-phase endometrium was seen in 24 out of 29 (82.8%) controls, but in only 6 out of 21 (28.6%) of cases with RPL; Type I and II defects were seen in 10.3 and 6.9% of controls, while present in 52.4 and 19.1% of cases, respectively (P = 0.0005; chi-square). Conclusions: Women with unexplained RPL had significantly reduced integrin expression compared to controls. Our findings underline the need for further molecular analysis of endometrial tissue in affected women.application/pdfengReproductive biology and endocrinology. London. Vol. 12 (Jun. 2014), 5 p.Integrina beta 3GravidezDoencas uterinasBeta3 integrinRecurrent pregnancy lossEndometrial datingEndometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy lossEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000953079.pdf000953079.pdfTexto completo (inglês)application/pdf318395http://www.lume.ufrgs.br/bitstream/10183/111824/1/000953079.pdf85286b4599a01576e631aa27e701e5efMD51TEXT000953079.pdf.txt000953079.pdf.txtExtracted Texttext/plain27750http://www.lume.ufrgs.br/bitstream/10183/111824/2/000953079.pdf.txt3413c2673f64c4e6ac17ca1f5c3601b5MD52THUMBNAIL000953079.pdf.jpg000953079.pdf.jpgGenerated Thumbnailimage/jpeg2006http://www.lume.ufrgs.br/bitstream/10183/111824/3/000953079.pdf.jpg1d5bf98df60be8e00b2873de4aa2cacaMD5310183/1118242021-05-07 05:03:01.682369oai:www.lume.ufrgs.br:10183/111824Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-05-07T08:03:01Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss |
title |
Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss |
spellingShingle |
Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss Germeyer, Ariane Integrina beta 3 Gravidez Doencas uterinas Beta3 integrin Recurrent pregnancy loss Endometrial dating |
title_short |
Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss |
title_full |
Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss |
title_fullStr |
Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss |
title_full_unstemmed |
Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss |
title_sort |
Endometrial beta3 integrin profile reflects endometrial receptivity defects in women with unexplained recurrent pregnancy loss |
author |
Germeyer, Ariane |
author_facet |
Germeyer, Ariane Savaris, Ricardo Francalacci Jauckus, Julia Lessey, Bruce Arthur |
author_role |
author |
author2 |
Savaris, Ricardo Francalacci Jauckus, Julia Lessey, Bruce Arthur |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Germeyer, Ariane Savaris, Ricardo Francalacci Jauckus, Julia Lessey, Bruce Arthur |
dc.subject.por.fl_str_mv |
Integrina beta 3 Gravidez Doencas uterinas |
topic |
Integrina beta 3 Gravidez Doencas uterinas Beta3 integrin Recurrent pregnancy loss Endometrial dating |
dc.subject.eng.fl_str_mv |
Beta3 integrin Recurrent pregnancy loss Endometrial dating |
description |
Background: The pathophysiology of recurrent pregnancy loss (RPL) is still unknown in 50% of the cases. Herein we measure the expression of beta3 integrin subunit, a well-known implantation marker, in women with or without RPL and correlate it with the histological dating of the endometrial tissue. Methods: LH-timed endometrial biopsies were obtained from cases (RPL; n = 21, age 33.9+/−4.7) and healthy controls (n = 29; age 29.8+/−4.1) during the mid-secretory phase (post ovulatory day: 8 to 10). Endometrial samples were timed histologically according to Noyes’ criteria and underwent immunohistochemical staining for beta3 integrin expression. For statistical analysis the semi-quantitative HSCORE was assessed. Type I (beta3 negative in an out-of-phase endometrium) and Type II defect (beta3 negative in an in-phase endometrium) were also analysed. Statistical analysis was done with Student t-test, Mann Whitney U test, ANCOVA and chi square for trend. Significance was set as P < 0.05. Results: The mean (SD) age in controls was lower compared to cases [(29.8 (4.1) vs. 33.9 (4.7) – P = 0.001; Student t-test)]. The median (range) expression of beta3 integrin in controls and cases was 1.94 (0 to 3.5) vs. 0.82 (0 to 3.6), respectively (P = 0.001; Mann Whitney U test). Significance was still significant after adjusting for age (P = 0.03;ANCOVA). The normal positive staining > =0.7 of beta3 integrin subunit and in-phase endometrium was seen in 24 out of 29 (82.8%) controls, but in only 6 out of 21 (28.6%) of cases with RPL; Type I and II defects were seen in 10.3 and 6.9% of controls, while present in 52.4 and 19.1% of cases, respectively (P = 0.0005; chi-square). Conclusions: Women with unexplained RPL had significantly reduced integrin expression compared to controls. Our findings underline the need for further molecular analysis of endometrial tissue in affected women. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
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2015-03-07T01:57:09Z |
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1477-7827 |
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000953079 |
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http://hdl.handle.net/10183/111824 |
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eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Reproductive biology and endocrinology. London. Vol. 12 (Jun. 2014), 5 p. |
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openAccess |
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