Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp

Detalhes bibliográficos
Autor(a) principal: Veríssimo, Carolina de Marco
Data de Publicação: 2013
Outros Autores: Maschio, Vinicius José, Correa, Ana Paula Folmer, Brandelli, Adriano, Rott, Marilise Brittes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/100155
Resumo: Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba, isolates from the environment and clinical cases were evaluated. The in vivo model was effective in establishing the infection and differentiating the pathogenicity of the isolates and re-isolates. The amoebae cultured in the laboratory for long periods were less virulent than those that were recently isolated, confirming the importance of passing Acanthamoeba strains in animal models.
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spelling Veríssimo, Carolina de MarcoMaschio, Vinicius JoséCorrea, Ana Paula FolmerBrandelli, AdrianoRott, Marilise Brittes2014-08-12T02:10:53Z20130074-0276http://hdl.handle.net/10183/100155000921935Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba, isolates from the environment and clinical cases were evaluated. The in vivo model was effective in establishing the infection and differentiating the pathogenicity of the isolates and re-isolates. The amoebae cultured in the laboratory for long periods were less virulent than those that were recently isolated, confirming the importance of passing Acanthamoeba strains in animal models.application/pdfengMemórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 108, n. 7 (nov. 2013), p. 832-835AcanthamoebaVirulênciaVirulenceIn vivoInfection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba sppinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000921935.pdf.txt000921935.pdf.txtExtracted Texttext/plain18810http://www.lume.ufrgs.br/bitstream/10183/100155/2/000921935.pdf.txt852b2fa5479906b6418fa46096046dabMD52ORIGINAL000921935.pdf000921935.pdfTexto completo (inglês)application/pdf884484http://www.lume.ufrgs.br/bitstream/10183/100155/1/000921935.pdf82d9d0bfc6b5775669775c52e6e10b82MD5110183/1001552024-01-31 06:01:22.597271oai:www.lume.ufrgs.br:10183/100155Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-01-31T08:01:22Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
spellingShingle Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
Veríssimo, Carolina de Marco
Acanthamoeba
Virulência
Virulence
In vivo
title_short Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title_full Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title_fullStr Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title_full_unstemmed Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
title_sort Infection in a rat model reactivates attenuated virulence after long-term axenic culture of Acanthamoeba spp
author Veríssimo, Carolina de Marco
author_facet Veríssimo, Carolina de Marco
Maschio, Vinicius José
Correa, Ana Paula Folmer
Brandelli, Adriano
Rott, Marilise Brittes
author_role author
author2 Maschio, Vinicius José
Correa, Ana Paula Folmer
Brandelli, Adriano
Rott, Marilise Brittes
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Veríssimo, Carolina de Marco
Maschio, Vinicius José
Correa, Ana Paula Folmer
Brandelli, Adriano
Rott, Marilise Brittes
dc.subject.por.fl_str_mv Acanthamoeba
Virulência
topic Acanthamoeba
Virulência
Virulence
In vivo
dc.subject.eng.fl_str_mv Virulence
In vivo
description Prolonged culturing of many microorganisms leads to the loss of virulence and a reduction of their infective capacity. However, little is known about the changes in the pathogenic strains of Acanthamoeba after long culture periods. Our study evaluated the effect of prolonged culturing on the invasiveness of different isolates of Acanthamoeba in an in vivo rat model. ATCC strains of Acanthamoeba, isolates from the environment and clinical cases were evaluated. The in vivo model was effective in establishing the infection and differentiating the pathogenicity of the isolates and re-isolates. The amoebae cultured in the laboratory for long periods were less virulent than those that were recently isolated, confirming the importance of passing Acanthamoeba strains in animal models.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-08-12T02:10:53Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/100155
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.nrb.pt_BR.fl_str_mv 000921935
identifier_str_mv 0074-0276
000921935
url http://hdl.handle.net/10183/100155
dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Memórias do Instituto Oswaldo Cruz : an international journal of biological and biomedical research. Vol. 108, n. 7 (nov. 2013), p. 832-835
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