Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/49796 |
Resumo: | OBJECTIVE: The purpose of this study was to describe the probable mechanism of the volume increase of laparoscopically harvested omentum flaps used to treat breast deformities. METHODS: A histological analysis of omentum samples was performed to study the volume increase of laparoscopically harvested omentum flaps. Samples were harvested immediately after the transposition of the omentum from the abdominal cavity to the breast region and during the second surgical procedure for breast symmetrization of eight patients submitted to the transposition of the omentum flap. Changes in the morphometric measurements of the adipocytes (perimeter, diameter, and area), microvascular density (as measured by the CD31 endothelial marker), and immunohistochemical expression of VEGF were documented. RESULTS: The increases in adipocyte size and microvascular density were statistically significant (P # 0.012). The expression levels of VEGF were lower in the second set of samples when compared to the first set, but the differences were not statistically significant (P , 0.093). CONCLUSION: These results demonstrate an increase in cellular volume as measured by adipocyte perimeter, diameter, and area. Moreover, the increase in the number of vessels in the second set of samples suggests that neoangiogenesis was stimulated by the initial increase in VEGF expression levels observed in the first set of samples. The increase in VEGF expression in the flap may have been caused by adipocyte hypertrophy resulting from neoangiogenesis. |
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Costa, Sirlei dos SantosBlotta, Rosa MariaMeurer, LuíseEdelweiss, Maria Isabel Albano2012-06-27T01:32:05Z20111807-5932http://hdl.handle.net/10183/49796000778933OBJECTIVE: The purpose of this study was to describe the probable mechanism of the volume increase of laparoscopically harvested omentum flaps used to treat breast deformities. METHODS: A histological analysis of omentum samples was performed to study the volume increase of laparoscopically harvested omentum flaps. Samples were harvested immediately after the transposition of the omentum from the abdominal cavity to the breast region and during the second surgical procedure for breast symmetrization of eight patients submitted to the transposition of the omentum flap. Changes in the morphometric measurements of the adipocytes (perimeter, diameter, and area), microvascular density (as measured by the CD31 endothelial marker), and immunohistochemical expression of VEGF were documented. RESULTS: The increases in adipocyte size and microvascular density were statistically significant (P # 0.012). The expression levels of VEGF were lower in the second set of samples when compared to the first set, but the differences were not statistically significant (P , 0.093). CONCLUSION: These results demonstrate an increase in cellular volume as measured by adipocyte perimeter, diameter, and area. Moreover, the increase in the number of vessels in the second set of samples suggests that neoangiogenesis was stimulated by the initial increase in VEGF expression levels observed in the first set of samples. The increase in VEGF expression in the flap may have been caused by adipocyte hypertrophy resulting from neoangiogenesis.application/pdfengClinics. São Paulo. Vol. 66, n. 2 (fev. 2011), p. 307-312AdipócitosNeovascularização patológicaHipertrofiaAdipocyteAngiogenesisAdipocyte hypertrophyVegfAdipose tissueAdipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary studyinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000778933.pdf000778933.pdfTexto completo (inglês)application/pdf2134225http://www.lume.ufrgs.br/bitstream/10183/49796/1/000778933.pdf3438243b493253c9ae48fb72961428e7MD51TEXT000778933.pdf.txt000778933.pdf.txtExtracted Texttext/plain26251http://www.lume.ufrgs.br/bitstream/10183/49796/2/000778933.pdf.txte050217f1e517f22906573c10eeecf33MD52THUMBNAIL000778933.pdf.jpg000778933.pdf.jpgGenerated Thumbnailimage/jpeg2016http://www.lume.ufrgs.br/bitstream/10183/49796/3/000778933.pdf.jpg278000b3dc1bd13f96798fc400a99a92MD5310183/497962018-10-10 07:40:16.9oai:www.lume.ufrgs.br:10183/49796Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-10T10:40:16Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study |
title |
Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study |
spellingShingle |
Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study Costa, Sirlei dos Santos Adipócitos Neovascularização patológica Hipertrofia Adipocyte Angiogenesis Adipocyte hypertrophy Vegf Adipose tissue |
title_short |
Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study |
title_full |
Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study |
title_fullStr |
Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study |
title_full_unstemmed |
Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study |
title_sort |
Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study |
author |
Costa, Sirlei dos Santos |
author_facet |
Costa, Sirlei dos Santos Blotta, Rosa Maria Meurer, Luíse Edelweiss, Maria Isabel Albano |
author_role |
author |
author2 |
Blotta, Rosa Maria Meurer, Luíse Edelweiss, Maria Isabel Albano |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Costa, Sirlei dos Santos Blotta, Rosa Maria Meurer, Luíse Edelweiss, Maria Isabel Albano |
dc.subject.por.fl_str_mv |
Adipócitos Neovascularização patológica Hipertrofia |
topic |
Adipócitos Neovascularização patológica Hipertrofia Adipocyte Angiogenesis Adipocyte hypertrophy Vegf Adipose tissue |
dc.subject.eng.fl_str_mv |
Adipocyte Angiogenesis Adipocyte hypertrophy Vegf Adipose tissue |
description |
OBJECTIVE: The purpose of this study was to describe the probable mechanism of the volume increase of laparoscopically harvested omentum flaps used to treat breast deformities. METHODS: A histological analysis of omentum samples was performed to study the volume increase of laparoscopically harvested omentum flaps. Samples were harvested immediately after the transposition of the omentum from the abdominal cavity to the breast region and during the second surgical procedure for breast symmetrization of eight patients submitted to the transposition of the omentum flap. Changes in the morphometric measurements of the adipocytes (perimeter, diameter, and area), microvascular density (as measured by the CD31 endothelial marker), and immunohistochemical expression of VEGF were documented. RESULTS: The increases in adipocyte size and microvascular density were statistically significant (P # 0.012). The expression levels of VEGF were lower in the second set of samples when compared to the first set, but the differences were not statistically significant (P , 0.093). CONCLUSION: These results demonstrate an increase in cellular volume as measured by adipocyte perimeter, diameter, and area. Moreover, the increase in the number of vessels in the second set of samples suggests that neoangiogenesis was stimulated by the initial increase in VEGF expression levels observed in the first set of samples. The increase in VEGF expression in the flap may have been caused by adipocyte hypertrophy resulting from neoangiogenesis. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2012-06-27T01:32:05Z |
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http://hdl.handle.net/10183/49796 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Clinics. São Paulo. Vol. 66, n. 2 (fev. 2011), p. 307-312 |
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openAccess |
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