Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study

Detalhes bibliográficos
Autor(a) principal: Costa, Sirlei dos Santos
Data de Publicação: 2011
Outros Autores: Blotta, Rosa Maria, Meurer, Luíse, Edelweiss, Maria Isabel Albano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/49796
Resumo: OBJECTIVE: The purpose of this study was to describe the probable mechanism of the volume increase of laparoscopically harvested omentum flaps used to treat breast deformities. METHODS: A histological analysis of omentum samples was performed to study the volume increase of laparoscopically harvested omentum flaps. Samples were harvested immediately after the transposition of the omentum from the abdominal cavity to the breast region and during the second surgical procedure for breast symmetrization of eight patients submitted to the transposition of the omentum flap. Changes in the morphometric measurements of the adipocytes (perimeter, diameter, and area), microvascular density (as measured by the CD31 endothelial marker), and immunohistochemical expression of VEGF were documented. RESULTS: The increases in adipocyte size and microvascular density were statistically significant (P # 0.012). The expression levels of VEGF were lower in the second set of samples when compared to the first set, but the differences were not statistically significant (P , 0.093). CONCLUSION: These results demonstrate an increase in cellular volume as measured by adipocyte perimeter, diameter, and area. Moreover, the increase in the number of vessels in the second set of samples suggests that neoangiogenesis was stimulated by the initial increase in VEGF expression levels observed in the first set of samples. The increase in VEGF expression in the flap may have been caused by adipocyte hypertrophy resulting from neoangiogenesis.
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spelling Costa, Sirlei dos SantosBlotta, Rosa MariaMeurer, LuíseEdelweiss, Maria Isabel Albano2012-06-27T01:32:05Z20111807-5932http://hdl.handle.net/10183/49796000778933OBJECTIVE: The purpose of this study was to describe the probable mechanism of the volume increase of laparoscopically harvested omentum flaps used to treat breast deformities. METHODS: A histological analysis of omentum samples was performed to study the volume increase of laparoscopically harvested omentum flaps. Samples were harvested immediately after the transposition of the omentum from the abdominal cavity to the breast region and during the second surgical procedure for breast symmetrization of eight patients submitted to the transposition of the omentum flap. Changes in the morphometric measurements of the adipocytes (perimeter, diameter, and area), microvascular density (as measured by the CD31 endothelial marker), and immunohistochemical expression of VEGF were documented. RESULTS: The increases in adipocyte size and microvascular density were statistically significant (P # 0.012). The expression levels of VEGF were lower in the second set of samples when compared to the first set, but the differences were not statistically significant (P , 0.093). CONCLUSION: These results demonstrate an increase in cellular volume as measured by adipocyte perimeter, diameter, and area. Moreover, the increase in the number of vessels in the second set of samples suggests that neoangiogenesis was stimulated by the initial increase in VEGF expression levels observed in the first set of samples. The increase in VEGF expression in the flap may have been caused by adipocyte hypertrophy resulting from neoangiogenesis.application/pdfengClinics. São Paulo. Vol. 66, n. 2 (fev. 2011), p. 307-312AdipócitosNeovascularização patológicaHipertrofiaAdipocyteAngiogenesisAdipocyte hypertrophyVegfAdipose tissueAdipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary studyinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000778933.pdf000778933.pdfTexto completo (inglês)application/pdf2134225http://www.lume.ufrgs.br/bitstream/10183/49796/1/000778933.pdf3438243b493253c9ae48fb72961428e7MD51TEXT000778933.pdf.txt000778933.pdf.txtExtracted Texttext/plain26251http://www.lume.ufrgs.br/bitstream/10183/49796/2/000778933.pdf.txte050217f1e517f22906573c10eeecf33MD52THUMBNAIL000778933.pdf.jpg000778933.pdf.jpgGenerated Thumbnailimage/jpeg2016http://www.lume.ufrgs.br/bitstream/10183/49796/3/000778933.pdf.jpg278000b3dc1bd13f96798fc400a99a92MD5310183/497962018-10-10 07:40:16.9oai:www.lume.ufrgs.br:10183/49796Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-10T10:40:16Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study
title Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study
spellingShingle Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study
Costa, Sirlei dos Santos
Adipócitos
Neovascularização patológica
Hipertrofia
Adipocyte
Angiogenesis
Adipocyte hypertrophy
Vegf
Adipose tissue
title_short Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study
title_full Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study
title_fullStr Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study
title_full_unstemmed Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study
title_sort Adipocyte morphometric evaluation and angiogenesis in the omentum transposed to the breast : a preliminary study
author Costa, Sirlei dos Santos
author_facet Costa, Sirlei dos Santos
Blotta, Rosa Maria
Meurer, Luíse
Edelweiss, Maria Isabel Albano
author_role author
author2 Blotta, Rosa Maria
Meurer, Luíse
Edelweiss, Maria Isabel Albano
author2_role author
author
author
dc.contributor.author.fl_str_mv Costa, Sirlei dos Santos
Blotta, Rosa Maria
Meurer, Luíse
Edelweiss, Maria Isabel Albano
dc.subject.por.fl_str_mv Adipócitos
Neovascularização patológica
Hipertrofia
topic Adipócitos
Neovascularização patológica
Hipertrofia
Adipocyte
Angiogenesis
Adipocyte hypertrophy
Vegf
Adipose tissue
dc.subject.eng.fl_str_mv Adipocyte
Angiogenesis
Adipocyte hypertrophy
Vegf
Adipose tissue
description OBJECTIVE: The purpose of this study was to describe the probable mechanism of the volume increase of laparoscopically harvested omentum flaps used to treat breast deformities. METHODS: A histological analysis of omentum samples was performed to study the volume increase of laparoscopically harvested omentum flaps. Samples were harvested immediately after the transposition of the omentum from the abdominal cavity to the breast region and during the second surgical procedure for breast symmetrization of eight patients submitted to the transposition of the omentum flap. Changes in the morphometric measurements of the adipocytes (perimeter, diameter, and area), microvascular density (as measured by the CD31 endothelial marker), and immunohistochemical expression of VEGF were documented. RESULTS: The increases in adipocyte size and microvascular density were statistically significant (P # 0.012). The expression levels of VEGF were lower in the second set of samples when compared to the first set, but the differences were not statistically significant (P , 0.093). CONCLUSION: These results demonstrate an increase in cellular volume as measured by adipocyte perimeter, diameter, and area. Moreover, the increase in the number of vessels in the second set of samples suggests that neoangiogenesis was stimulated by the initial increase in VEGF expression levels observed in the first set of samples. The increase in VEGF expression in the flap may have been caused by adipocyte hypertrophy resulting from neoangiogenesis.
publishDate 2011
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dc.relation.ispartof.pt_BR.fl_str_mv Clinics. São Paulo. Vol. 66, n. 2 (fev. 2011), p. 307-312
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