Resveratrol prevents ammonia toxicity in astroglial cells
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225329 |
Resumo: | Ammonia is implicated as a neurotoxin in brain metabolic disorders associated with hyperammonemia. Acute ammonia toxicity can be mediated by an excitotoxic mechanism, oxidative stress and nitric oxide (NO) production. Astrocytes interact with neurons, providing metabolic support and protecting against oxidative stress and excitotoxicity. Astrocytes also convert excess ammonia and glutamate into glutamine via glutamine synthetase (GS). Resveratrol, a polyphenol found in grapes and red wines, exhibits antioxidant and anti-inflammatory properties and modulates glial functions, such as glutamate metabolism. We investigated the effect of resveratrol on the production of reactive oxygen species (ROS), GS activity, S100B secretion, TNF-a, IL-1b and IL-6 levels in astroglial cells exposed to ammonia. Ammonia induced oxidative stress, decreased GS activity and increased cytokines release, probably by a mechanism dependent on protein kinase A (PKA) and extracellular signal-regulated kinase (ERK) pathways. Resveratrol prevented ammonia toxicity by modulating oxidative stress, glial and inflammatory responses. The ERK and nuclear factor-kB (NF-kB) are involved in the protective effect of resveratrol on cytokines proinflammatory release. In contrast, other antioxidants (e.g., ascorbic acid and trolox) were not effective against hyperammonemia. Thus, resveratrol could be used to protect against ammonia-induced neurotoxicity. |
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Bobermin, Larissa DanieleQuincozes-Santos, AndréGuerra, Maria Cristina Azambuja Barea da SilveiraLeite, Marina ConcliSouza, Diogo Onofre Gomes deGoncalves, Carlos Alberto SaraivaGottfried, Carmem Juracy Silveira2021-08-06T04:43:00Z20121932-6203http://hdl.handle.net/10183/225329000918972Ammonia is implicated as a neurotoxin in brain metabolic disorders associated with hyperammonemia. Acute ammonia toxicity can be mediated by an excitotoxic mechanism, oxidative stress and nitric oxide (NO) production. Astrocytes interact with neurons, providing metabolic support and protecting against oxidative stress and excitotoxicity. Astrocytes also convert excess ammonia and glutamate into glutamine via glutamine synthetase (GS). Resveratrol, a polyphenol found in grapes and red wines, exhibits antioxidant and anti-inflammatory properties and modulates glial functions, such as glutamate metabolism. We investigated the effect of resveratrol on the production of reactive oxygen species (ROS), GS activity, S100B secretion, TNF-a, IL-1b and IL-6 levels in astroglial cells exposed to ammonia. Ammonia induced oxidative stress, decreased GS activity and increased cytokines release, probably by a mechanism dependent on protein kinase A (PKA) and extracellular signal-regulated kinase (ERK) pathways. Resveratrol prevented ammonia toxicity by modulating oxidative stress, glial and inflammatory responses. The ERK and nuclear factor-kB (NF-kB) are involved in the protective effect of resveratrol on cytokines proinflammatory release. In contrast, other antioxidants (e.g., ascorbic acid and trolox) were not effective against hyperammonemia. Thus, resveratrol could be used to protect against ammonia-induced neurotoxicity.application/pdfengPLoS ONE. San Francisco. Vol. 7, no. 12 (Dec. 2012), e52164, 12 f.Estresse oxidativoAmôniaCitocinasAminoácidosCompostos de nitrogênioResveratrol prevents ammonia toxicity in astroglial cellsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000918972.pdf.txt000918972.pdf.txtExtracted Texttext/plain59940http://www.lume.ufrgs.br/bitstream/10183/225329/2/000918972.pdf.txtd018697795978df90e5df57576fb6377MD52ORIGINAL000918972.pdfTexto completo (inglês)application/pdf645315http://www.lume.ufrgs.br/bitstream/10183/225329/1/000918972.pdf894f961e03d6839d8824d92df531eb8bMD5110183/2253292023-01-20 06:00:47.850059oai:www.lume.ufrgs.br:10183/225329Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-01-20T08:00:47Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Resveratrol prevents ammonia toxicity in astroglial cells |
title |
Resveratrol prevents ammonia toxicity in astroglial cells |
spellingShingle |
Resveratrol prevents ammonia toxicity in astroglial cells Bobermin, Larissa Daniele Estresse oxidativo Amônia Citocinas Aminoácidos Compostos de nitrogênio |
title_short |
Resveratrol prevents ammonia toxicity in astroglial cells |
title_full |
Resveratrol prevents ammonia toxicity in astroglial cells |
title_fullStr |
Resveratrol prevents ammonia toxicity in astroglial cells |
title_full_unstemmed |
Resveratrol prevents ammonia toxicity in astroglial cells |
title_sort |
Resveratrol prevents ammonia toxicity in astroglial cells |
author |
Bobermin, Larissa Daniele |
author_facet |
Bobermin, Larissa Daniele Quincozes-Santos, André Guerra, Maria Cristina Azambuja Barea da Silveira Leite, Marina Concli Souza, Diogo Onofre Gomes de Goncalves, Carlos Alberto Saraiva Gottfried, Carmem Juracy Silveira |
author_role |
author |
author2 |
Quincozes-Santos, André Guerra, Maria Cristina Azambuja Barea da Silveira Leite, Marina Concli Souza, Diogo Onofre Gomes de Goncalves, Carlos Alberto Saraiva Gottfried, Carmem Juracy Silveira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Bobermin, Larissa Daniele Quincozes-Santos, André Guerra, Maria Cristina Azambuja Barea da Silveira Leite, Marina Concli Souza, Diogo Onofre Gomes de Goncalves, Carlos Alberto Saraiva Gottfried, Carmem Juracy Silveira |
dc.subject.por.fl_str_mv |
Estresse oxidativo Amônia Citocinas Aminoácidos Compostos de nitrogênio |
topic |
Estresse oxidativo Amônia Citocinas Aminoácidos Compostos de nitrogênio |
description |
Ammonia is implicated as a neurotoxin in brain metabolic disorders associated with hyperammonemia. Acute ammonia toxicity can be mediated by an excitotoxic mechanism, oxidative stress and nitric oxide (NO) production. Astrocytes interact with neurons, providing metabolic support and protecting against oxidative stress and excitotoxicity. Astrocytes also convert excess ammonia and glutamate into glutamine via glutamine synthetase (GS). Resveratrol, a polyphenol found in grapes and red wines, exhibits antioxidant and anti-inflammatory properties and modulates glial functions, such as glutamate metabolism. We investigated the effect of resveratrol on the production of reactive oxygen species (ROS), GS activity, S100B secretion, TNF-a, IL-1b and IL-6 levels in astroglial cells exposed to ammonia. Ammonia induced oxidative stress, decreased GS activity and increased cytokines release, probably by a mechanism dependent on protein kinase A (PKA) and extracellular signal-regulated kinase (ERK) pathways. Resveratrol prevented ammonia toxicity by modulating oxidative stress, glial and inflammatory responses. The ERK and nuclear factor-kB (NF-kB) are involved in the protective effect of resveratrol on cytokines proinflammatory release. In contrast, other antioxidants (e.g., ascorbic acid and trolox) were not effective against hyperammonemia. Thus, resveratrol could be used to protect against ammonia-induced neurotoxicity. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2021-08-06T04:43:00Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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http://hdl.handle.net/10183/225329 |
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1932-6203 |
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000918972 |
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http://hdl.handle.net/10183/225329 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PLoS ONE. San Francisco. Vol. 7, no. 12 (Dec. 2012), e52164, 12 f. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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