Quantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compound
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/149733 |
Resumo: | Plants from the genus Pterocaulon are popularly used as antifungal and wound-healing agents. Such activities have been related to coumarins, which are abundant in those plants. Coumarins are soluble in organic solvents, such as hexane and dichloromethane, and some of them are also soluble in hot water. Considering that infusion and decoctions of these plants are used in traditional medicine, the aim of this study was to identify and quantify the coumarins in the aqueous extract of Pterocaulon balansae. The aqueous extract was obtained by dynamic maceration and the compounds were characterized by UPLC-UV-MS analysis. A new coumarin and 5-methoxy-6,7-methylenedioxycoumarin, used for validation of the analytical HPLC method were obtained by partition of the aqueous extract with n-hexane. The HPLC method validated was linear, specific, and precise. Seven coumarins were characterized in the aqueous extract in a range of 0.584–54 mg/g of dry plant material. The main compound, 5,6-dimethoxy-7-(3′-methyl-2′,3′-dihydroxybutyloxy)coumarin, is described for the first time in P. balansae together with a new compound, 5,6-dimethoxy-7-(2′,3′- epoxy-3′-methylbutyloxy)coumarin. |
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Neves, Bruna MedeirosBarros, Francisco Maikon Corrêa deVon Poser, Gilsane LinoTeixeira, Helder Ferreira2016-11-19T02:16:41Z20151420-3049http://hdl.handle.net/10183/149733000985595Plants from the genus Pterocaulon are popularly used as antifungal and wound-healing agents. Such activities have been related to coumarins, which are abundant in those plants. Coumarins are soluble in organic solvents, such as hexane and dichloromethane, and some of them are also soluble in hot water. Considering that infusion and decoctions of these plants are used in traditional medicine, the aim of this study was to identify and quantify the coumarins in the aqueous extract of Pterocaulon balansae. The aqueous extract was obtained by dynamic maceration and the compounds were characterized by UPLC-UV-MS analysis. A new coumarin and 5-methoxy-6,7-methylenedioxycoumarin, used for validation of the analytical HPLC method were obtained by partition of the aqueous extract with n-hexane. The HPLC method validated was linear, specific, and precise. Seven coumarins were characterized in the aqueous extract in a range of 0.584–54 mg/g of dry plant material. The main compound, 5,6-dimethoxy-7-(3′-methyl-2′,3′-dihydroxybutyloxy)coumarin, is described for the first time in P. balansae together with a new compound, 5,6-dimethoxy-7-(2′,3′- epoxy-3′-methylbutyloxy)coumarin.application/pdfengMolecules. Basel. Vol. 20, no.10 (2015), p. 18083-18094FarmáciaCoumarinPterocaulon balansaeHPLCUPLC-UV-MS5-methoxy-6,7- methylenedioxycoumarin5,6-dimethoxy-7-(2′,3′-epoxy-3′-methylbutyloxy)coumarinQuantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compoundEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000985595.pdf000985595.pdfTexto completo (inglês)application/pdf776069http://www.lume.ufrgs.br/bitstream/10183/149733/1/000985595.pdffe102947d04c7328d4a51e9ae4ef5e43MD51TEXT000985595.pdf.txt000985595.pdf.txtExtracted Texttext/plain27957http://www.lume.ufrgs.br/bitstream/10183/149733/2/000985595.pdf.txt5ef71af4d02c2533746fb2daff6f3e1fMD52THUMBNAIL000985595.pdf.jpg000985595.pdf.jpgGenerated Thumbnailimage/jpeg2065http://www.lume.ufrgs.br/bitstream/10183/149733/3/000985595.pdf.jpg0f4c6300f618b2aaa8d9b93d6d730414MD5310183/1497332023-11-06 04:31:37.726325oai:www.lume.ufrgs.br:10183/149733Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-11-06T06:31:37Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Quantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compound |
title |
Quantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compound |
spellingShingle |
Quantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compound Neves, Bruna Medeiros Farmácia Coumarin Pterocaulon balansae HPLC UPLC-UV-MS 5-methoxy-6,7- methylenedioxycoumarin 5,6-dimethoxy-7-(2′,3′-epoxy-3′-methylbutyloxy)coumarin |
title_short |
Quantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compound |
title_full |
Quantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compound |
title_fullStr |
Quantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compound |
title_full_unstemmed |
Quantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compound |
title_sort |
Quantification of coumarins in aqueous extract of Pterocaulon balansae (Asteraceae) and characterization of a new compound |
author |
Neves, Bruna Medeiros |
author_facet |
Neves, Bruna Medeiros Barros, Francisco Maikon Corrêa de Von Poser, Gilsane Lino Teixeira, Helder Ferreira |
author_role |
author |
author2 |
Barros, Francisco Maikon Corrêa de Von Poser, Gilsane Lino Teixeira, Helder Ferreira |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Neves, Bruna Medeiros Barros, Francisco Maikon Corrêa de Von Poser, Gilsane Lino Teixeira, Helder Ferreira |
dc.subject.por.fl_str_mv |
Farmácia |
topic |
Farmácia Coumarin Pterocaulon balansae HPLC UPLC-UV-MS 5-methoxy-6,7- methylenedioxycoumarin 5,6-dimethoxy-7-(2′,3′-epoxy-3′-methylbutyloxy)coumarin |
dc.subject.eng.fl_str_mv |
Coumarin Pterocaulon balansae HPLC UPLC-UV-MS 5-methoxy-6,7- methylenedioxycoumarin 5,6-dimethoxy-7-(2′,3′-epoxy-3′-methylbutyloxy)coumarin |
description |
Plants from the genus Pterocaulon are popularly used as antifungal and wound-healing agents. Such activities have been related to coumarins, which are abundant in those plants. Coumarins are soluble in organic solvents, such as hexane and dichloromethane, and some of them are also soluble in hot water. Considering that infusion and decoctions of these plants are used in traditional medicine, the aim of this study was to identify and quantify the coumarins in the aqueous extract of Pterocaulon balansae. The aqueous extract was obtained by dynamic maceration and the compounds were characterized by UPLC-UV-MS analysis. A new coumarin and 5-methoxy-6,7-methylenedioxycoumarin, used for validation of the analytical HPLC method were obtained by partition of the aqueous extract with n-hexane. The HPLC method validated was linear, specific, and precise. Seven coumarins were characterized in the aqueous extract in a range of 0.584–54 mg/g of dry plant material. The main compound, 5,6-dimethoxy-7-(3′-methyl-2′,3′-dihydroxybutyloxy)coumarin, is described for the first time in P. balansae together with a new compound, 5,6-dimethoxy-7-(2′,3′- epoxy-3′-methylbutyloxy)coumarin. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2016-11-19T02:16:41Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/149733 |
dc.identifier.issn.pt_BR.fl_str_mv |
1420-3049 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000985595 |
identifier_str_mv |
1420-3049 000985595 |
url |
http://hdl.handle.net/10183/149733 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Molecules. Basel. Vol. 20, no.10 (2015), p. 18083-18094 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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