Endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/194186 |
Resumo: | Background. The aim of this study was to evaluate the use of liquid perfluorocarbon (PFC) as an adjuvant substance for lung preservation and assess its role in pulmonary protection after transplantation. Methods. Seventy-two rat lungs were flushed with low-potassium dextran (LPD) solution and randomized into three main groups: control with LPD alone and experimental with 3 (PFC3) and 7 mL/kg (PFC7) of endobronchial PFC instilled just after harvest. Each groupwas divided into four subgroups according to preservation time (3, 6, 12, and 24 hours). Afterwards, we performed lung transplantation using rat lungs preserved for 12 hours with LPD alone or with 7 mL/kg of endobronchial PFC. Results.There was a significant increase in oxidative stress in the control group at 6 h of cold ischemic time compared with the PFC3 and PFC7 groups.The apoptotic activity and NF-B expression were significantly higher in the control group compared with the PFC groups at 3, 12, and 24 h of cold preservation. After transplantation, the NF-B, iNOS, and nitrotyrosine expression as well as caspase 3 activity were significantly lower in the PFC groups. Conclusion. The use of endobronchial PFC as an adjuvant to the current preservation strategy improved graft viability. |
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Forgiarini Júnior, Luiz AlbertoHoland, Arthur Rodrigo RonconiForgiarini, Luiz FelipeRosa, Darlan Pase daMarroni, Norma Anair PossaCardoso, Paulo Francisco GuerreiroAndrade, Cristiano Feijó2019-05-11T02:38:19Z20150962-9351http://hdl.handle.net/10183/194186000977260Background. The aim of this study was to evaluate the use of liquid perfluorocarbon (PFC) as an adjuvant substance for lung preservation and assess its role in pulmonary protection after transplantation. Methods. Seventy-two rat lungs were flushed with low-potassium dextran (LPD) solution and randomized into three main groups: control with LPD alone and experimental with 3 (PFC3) and 7 mL/kg (PFC7) of endobronchial PFC instilled just after harvest. Each groupwas divided into four subgroups according to preservation time (3, 6, 12, and 24 hours). Afterwards, we performed lung transplantation using rat lungs preserved for 12 hours with LPD alone or with 7 mL/kg of endobronchial PFC. Results.There was a significant increase in oxidative stress in the control group at 6 h of cold ischemic time compared with the PFC3 and PFC7 groups.The apoptotic activity and NF-B expression were significantly higher in the control group compared with the PFC groups at 3, 12, and 24 h of cold preservation. After transplantation, the NF-B, iNOS, and nitrotyrosine expression as well as caspase 3 activity were significantly lower in the PFC groups. Conclusion. The use of endobronchial PFC as an adjuvant to the current preservation strategy improved graft viability.application/pdfengMediators of inflammation. New York. Volume 2013 (2013), ID 193484, 9 p.FluorcarbonetosInflamaçãoFarmacologiaTransplante de pulmãoPreservação de órgãosModelos animaisEndobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental modelEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000977260.pdf.txt000977260.pdf.txtExtracted Texttext/plain39495http://www.lume.ufrgs.br/bitstream/10183/194186/2/000977260.pdf.txt4909a2db68156c696c0bc2460652c4b6MD52ORIGINAL000977260.pdfTexto completo (inglês)application/pdf3358644http://www.lume.ufrgs.br/bitstream/10183/194186/1/000977260.pdf84b3bc9d2149c6be961e105cdb7c9757MD5110183/1941862023-06-29 03:31:08.797142oai:www.lume.ufrgs.br:10183/194186Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-06-29T06:31:08Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model |
title |
Endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model |
spellingShingle |
Endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model Forgiarini Júnior, Luiz Alberto Fluorcarbonetos Inflamação Farmacologia Transplante de pulmão Preservação de órgãos Modelos animais |
title_short |
Endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model |
title_full |
Endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model |
title_fullStr |
Endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model |
title_full_unstemmed |
Endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model |
title_sort |
Endobronchial perfluorocarbon reduces inflammatory activity before and after lung transplantation in an animal experimental model |
author |
Forgiarini Júnior, Luiz Alberto |
author_facet |
Forgiarini Júnior, Luiz Alberto Holand, Arthur Rodrigo Ronconi Forgiarini, Luiz Felipe Rosa, Darlan Pase da Marroni, Norma Anair Possa Cardoso, Paulo Francisco Guerreiro Andrade, Cristiano Feijó |
author_role |
author |
author2 |
Holand, Arthur Rodrigo Ronconi Forgiarini, Luiz Felipe Rosa, Darlan Pase da Marroni, Norma Anair Possa Cardoso, Paulo Francisco Guerreiro Andrade, Cristiano Feijó |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Forgiarini Júnior, Luiz Alberto Holand, Arthur Rodrigo Ronconi Forgiarini, Luiz Felipe Rosa, Darlan Pase da Marroni, Norma Anair Possa Cardoso, Paulo Francisco Guerreiro Andrade, Cristiano Feijó |
dc.subject.por.fl_str_mv |
Fluorcarbonetos Inflamação Farmacologia Transplante de pulmão Preservação de órgãos Modelos animais |
topic |
Fluorcarbonetos Inflamação Farmacologia Transplante de pulmão Preservação de órgãos Modelos animais |
description |
Background. The aim of this study was to evaluate the use of liquid perfluorocarbon (PFC) as an adjuvant substance for lung preservation and assess its role in pulmonary protection after transplantation. Methods. Seventy-two rat lungs were flushed with low-potassium dextran (LPD) solution and randomized into three main groups: control with LPD alone and experimental with 3 (PFC3) and 7 mL/kg (PFC7) of endobronchial PFC instilled just after harvest. Each groupwas divided into four subgroups according to preservation time (3, 6, 12, and 24 hours). Afterwards, we performed lung transplantation using rat lungs preserved for 12 hours with LPD alone or with 7 mL/kg of endobronchial PFC. Results.There was a significant increase in oxidative stress in the control group at 6 h of cold ischemic time compared with the PFC3 and PFC7 groups.The apoptotic activity and NF-B expression were significantly higher in the control group compared with the PFC groups at 3, 12, and 24 h of cold preservation. After transplantation, the NF-B, iNOS, and nitrotyrosine expression as well as caspase 3 activity were significantly lower in the PFC groups. Conclusion. The use of endobronchial PFC as an adjuvant to the current preservation strategy improved graft viability. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2019-05-11T02:38:19Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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http://hdl.handle.net/10183/194186 |
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0962-9351 |
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000977260 |
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http://hdl.handle.net/10183/194186 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Mediators of inflammation. New York. Volume 2013 (2013), ID 193484, 9 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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