Vitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained rats
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/165126 |
Resumo: | Exercise training intensity is the major variant that influences the relationship between exercise, redox balance, and immune response. Supplement intake is a common practice for oxidative stress prevention; the effects of vitamin A (VA) on exercise training are not yet described, even though this molecule exhibits antioxidant properties. We investigated the role of VA supplementation on redox and immune responses of adult Wistar rats subjected to swimming training. Animals were divided into four groups: sedentary, sedentary + VA, exercise training, and exercise training + VA. Over eight weeks, animals were submitted to intense swimming 5 times/week and a VA daily intake of 450 retinol equivalents/day. VA impaired the total serum antioxidant capacity acquired by exercise, with no change in interleukin-1 and tumor necrosis factor- levels. In skeletal muscle, VA caused lipid peroxidation and protein damage without differences in antioxidant enzyme activities; however, Western blot analysis showed that expression of superoxide dismutase-1 was downregulated, and upregulation of superoxide dismutase-2 induced by exercise was blunted by VA. Furthermore, VA supplementation decreased anti-inflammatory interleukin-10 and heat shock protein 70 expression, important factors for positive exercise adaptations and tissue damage prevention. Our data showed that VA supplementation did not confer any antioxidative and/or protective effects, attenuating exercise-acquired benefits in the skeletal muscle. |
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Petiz, Lyvia LintzmaierGirardi, Carolina SaibroBortolin, Rafael CalixtoKunzler, AliceGasparotto, JucianoRabelo, Thallita KellyMatté, CristianeMoreira, Jose Claudio FonsecaGelain, Daniel Pens2017-08-11T02:37:10Z20172072-6643http://hdl.handle.net/10183/165126001044443Exercise training intensity is the major variant that influences the relationship between exercise, redox balance, and immune response. Supplement intake is a common practice for oxidative stress prevention; the effects of vitamin A (VA) on exercise training are not yet described, even though this molecule exhibits antioxidant properties. We investigated the role of VA supplementation on redox and immune responses of adult Wistar rats subjected to swimming training. Animals were divided into four groups: sedentary, sedentary + VA, exercise training, and exercise training + VA. Over eight weeks, animals were submitted to intense swimming 5 times/week and a VA daily intake of 450 retinol equivalents/day. VA impaired the total serum antioxidant capacity acquired by exercise, with no change in interleukin-1 and tumor necrosis factor- levels. In skeletal muscle, VA caused lipid peroxidation and protein damage without differences in antioxidant enzyme activities; however, Western blot analysis showed that expression of superoxide dismutase-1 was downregulated, and upregulation of superoxide dismutase-2 induced by exercise was blunted by VA. Furthermore, VA supplementation decreased anti-inflammatory interleukin-10 and heat shock protein 70 expression, important factors for positive exercise adaptations and tissue damage prevention. Our data showed that VA supplementation did not confer any antioxidative and/or protective effects, attenuating exercise-acquired benefits in the skeletal muscle.application/pdfengNutrients. Basel, Switzerland. Vol. 9, no. 4, (Apr. 2017), E353, [16 p.]EnzimasAntioxidantesCitocinasEstresse oxidativoAntioxidant enzymesAntioxidant supplementsExerciseCytokinesVitaminVitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained ratsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001044443.pdf001044443.pdfTexto completo (inglês)application/pdf2074247http://www.lume.ufrgs.br/bitstream/10183/165126/1/001044443.pdfaad12ff016ac66a65e379c485c0bae86MD51TEXT001044443.pdf.txt001044443.pdf.txtExtracted Texttext/plain69004http://www.lume.ufrgs.br/bitstream/10183/165126/2/001044443.pdf.txta7edd6c4ac76807e7798d1dde35984d9MD52THUMBNAIL001044443.pdf.jpg001044443.pdf.jpgGenerated Thumbnailimage/jpeg1829http://www.lume.ufrgs.br/bitstream/10183/165126/3/001044443.pdf.jpg793cc5eae0f811544ca5409c345d4982MD5310183/1651262021-09-18 04:46:20.12861oai:www.lume.ufrgs.br:10183/165126Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-18T07:46:20Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Vitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained rats |
title |
Vitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained rats |
spellingShingle |
Vitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained rats Petiz, Lyvia Lintzmaier Enzimas Antioxidantes Citocinas Estresse oxidativo Antioxidant enzymes Antioxidant supplements Exercise Cytokines Vitamin |
title_short |
Vitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained rats |
title_full |
Vitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained rats |
title_fullStr |
Vitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained rats |
title_full_unstemmed |
Vitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained rats |
title_sort |
Vitamin A oral supplementation induces oxidative stress and suppresses IL-10 and HSP70 in skeletal muscle of trained rats |
author |
Petiz, Lyvia Lintzmaier |
author_facet |
Petiz, Lyvia Lintzmaier Girardi, Carolina Saibro Bortolin, Rafael Calixto Kunzler, Alice Gasparotto, Juciano Rabelo, Thallita Kelly Matté, Cristiane Moreira, Jose Claudio Fonseca Gelain, Daniel Pens |
author_role |
author |
author2 |
Girardi, Carolina Saibro Bortolin, Rafael Calixto Kunzler, Alice Gasparotto, Juciano Rabelo, Thallita Kelly Matté, Cristiane Moreira, Jose Claudio Fonseca Gelain, Daniel Pens |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Petiz, Lyvia Lintzmaier Girardi, Carolina Saibro Bortolin, Rafael Calixto Kunzler, Alice Gasparotto, Juciano Rabelo, Thallita Kelly Matté, Cristiane Moreira, Jose Claudio Fonseca Gelain, Daniel Pens |
dc.subject.por.fl_str_mv |
Enzimas Antioxidantes Citocinas Estresse oxidativo |
topic |
Enzimas Antioxidantes Citocinas Estresse oxidativo Antioxidant enzymes Antioxidant supplements Exercise Cytokines Vitamin |
dc.subject.eng.fl_str_mv |
Antioxidant enzymes Antioxidant supplements Exercise Cytokines Vitamin |
description |
Exercise training intensity is the major variant that influences the relationship between exercise, redox balance, and immune response. Supplement intake is a common practice for oxidative stress prevention; the effects of vitamin A (VA) on exercise training are not yet described, even though this molecule exhibits antioxidant properties. We investigated the role of VA supplementation on redox and immune responses of adult Wistar rats subjected to swimming training. Animals were divided into four groups: sedentary, sedentary + VA, exercise training, and exercise training + VA. Over eight weeks, animals were submitted to intense swimming 5 times/week and a VA daily intake of 450 retinol equivalents/day. VA impaired the total serum antioxidant capacity acquired by exercise, with no change in interleukin-1 and tumor necrosis factor- levels. In skeletal muscle, VA caused lipid peroxidation and protein damage without differences in antioxidant enzyme activities; however, Western blot analysis showed that expression of superoxide dismutase-1 was downregulated, and upregulation of superoxide dismutase-2 induced by exercise was blunted by VA. Furthermore, VA supplementation decreased anti-inflammatory interleukin-10 and heat shock protein 70 expression, important factors for positive exercise adaptations and tissue damage prevention. Our data showed that VA supplementation did not confer any antioxidative and/or protective effects, attenuating exercise-acquired benefits in the skeletal muscle. |
publishDate |
2017 |
dc.date.accessioned.fl_str_mv |
2017-08-11T02:37:10Z |
dc.date.issued.fl_str_mv |
2017 |
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Estrangeiro info:eu-repo/semantics/article |
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2072-6643 |
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001044443 |
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http://hdl.handle.net/10183/165126 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Nutrients. Basel, Switzerland. Vol. 9, no. 4, (Apr. 2017), E353, [16 p.] |
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info:eu-repo/semantics/openAccess |
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openAccess |
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