Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21191 |
Resumo: | Typing techniques are essential for understanding hospital epidemiology, permitting the elucidation of the source of infection and routes of bacterial transmission. Although DNA-based techniques are the “gold standard” for the epidemiological study of Pseudomonas aeruginosa, antibiotic profiles and biochemical results are used because they are easy to perform and to interpret and relatively inexpensive. Antibiotypes (susceptibility profiles) and biotypes (biochemical profiles) were compared to genotypes established by DNA restriction enzyme analysis in 81 clinical isolates of P. aeruginosa from three hospitals in Porto Alegre, Brazil. The epidemiological relationship among patients was also evaluated. Susceptibility and restriction profiles were discrepant in more than 50% of the cases, and many antibiotypes were observed among isolates from the same genotype. Furthermore, susceptibility profiles did not allow the distinction of isolates from unrelated genotypes. Since a large number of isolates (63%) yielded the same biochemical results, only 10 biotypes were detected, showing that this typing method has a low discriminatory power. On the other hand, DNA restriction enzyme typing allowed us to establish 71 distinct types. Epidemiological data about the relation among P. aeruginosa isolates were not conclusive. The results of the present study indicate that the only method that can establish a clonal relation is DNA restriction enzyme typing, whereas the other methods may cause misleading interpretations and are inadequate to guide proper infection control measures. |
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Freitas, Ana Lucia Peixoto deBarth, Afonso Luis2010-04-24T04:15:39Z20040100-879Xhttp://hdl.handle.net/10183/21191000435655Typing techniques are essential for understanding hospital epidemiology, permitting the elucidation of the source of infection and routes of bacterial transmission. Although DNA-based techniques are the “gold standard” for the epidemiological study of Pseudomonas aeruginosa, antibiotic profiles and biochemical results are used because they are easy to perform and to interpret and relatively inexpensive. Antibiotypes (susceptibility profiles) and biotypes (biochemical profiles) were compared to genotypes established by DNA restriction enzyme analysis in 81 clinical isolates of P. aeruginosa from three hospitals in Porto Alegre, Brazil. The epidemiological relationship among patients was also evaluated. Susceptibility and restriction profiles were discrepant in more than 50% of the cases, and many antibiotypes were observed among isolates from the same genotype. Furthermore, susceptibility profiles did not allow the distinction of isolates from unrelated genotypes. Since a large number of isolates (63%) yielded the same biochemical results, only 10 biotypes were detected, showing that this typing method has a low discriminatory power. On the other hand, DNA restriction enzyme typing allowed us to establish 71 distinct types. Epidemiological data about the relation among P. aeruginosa isolates were not conclusive. The results of the present study indicate that the only method that can establish a clonal relation is DNA restriction enzyme typing, whereas the other methods may cause misleading interpretations and are inadequate to guide proper infection control measures.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 37, no. 1 (Jan. 2004), p.77-82Pseudomonas aeruginosaPseudomonas aeruginosaTypingGenotypingPhenotypic markersTyping of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotypeinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000435655.pdf000435655.pdfTexto completo (inglês)application/pdf455070http://www.lume.ufrgs.br/bitstream/10183/21191/1/000435655.pdfb3d0c257a9f3a0e58e6c6635c85857e8MD51TEXT000435655.pdf.txt000435655.pdf.txtExtracted Texttext/plain23531http://www.lume.ufrgs.br/bitstream/10183/21191/2/000435655.pdf.txt30c271bf91af3e1f0845c0a42a9d8cb1MD52THUMBNAIL000435655.pdf.jpg000435655.pdf.jpgGenerated Thumbnailimage/jpeg1697http://www.lume.ufrgs.br/bitstream/10183/21191/3/000435655.pdf.jpgdbbab2ccffe32d839a312fd448b579a2MD5310183/211912018-10-05 08:54:58.933oai:www.lume.ufrgs.br:10183/21191Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-05T11:54:58Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype |
title |
Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype |
spellingShingle |
Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype Freitas, Ana Lucia Peixoto de Pseudomonas aeruginosa Pseudomonas aeruginosa Typing Genotyping Phenotypic markers |
title_short |
Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype |
title_full |
Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype |
title_fullStr |
Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype |
title_full_unstemmed |
Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype |
title_sort |
Typing of Pseudomonas aeruginosa from hospitalized patients : a comparison of susceptibility and biochemical profiles with genotype |
author |
Freitas, Ana Lucia Peixoto de |
author_facet |
Freitas, Ana Lucia Peixoto de Barth, Afonso Luis |
author_role |
author |
author2 |
Barth, Afonso Luis |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Freitas, Ana Lucia Peixoto de Barth, Afonso Luis |
dc.subject.por.fl_str_mv |
Pseudomonas aeruginosa |
topic |
Pseudomonas aeruginosa Pseudomonas aeruginosa Typing Genotyping Phenotypic markers |
dc.subject.eng.fl_str_mv |
Pseudomonas aeruginosa Typing Genotyping Phenotypic markers |
description |
Typing techniques are essential for understanding hospital epidemiology, permitting the elucidation of the source of infection and routes of bacterial transmission. Although DNA-based techniques are the “gold standard” for the epidemiological study of Pseudomonas aeruginosa, antibiotic profiles and biochemical results are used because they are easy to perform and to interpret and relatively inexpensive. Antibiotypes (susceptibility profiles) and biotypes (biochemical profiles) were compared to genotypes established by DNA restriction enzyme analysis in 81 clinical isolates of P. aeruginosa from three hospitals in Porto Alegre, Brazil. The epidemiological relationship among patients was also evaluated. Susceptibility and restriction profiles were discrepant in more than 50% of the cases, and many antibiotypes were observed among isolates from the same genotype. Furthermore, susceptibility profiles did not allow the distinction of isolates from unrelated genotypes. Since a large number of isolates (63%) yielded the same biochemical results, only 10 biotypes were detected, showing that this typing method has a low discriminatory power. On the other hand, DNA restriction enzyme typing allowed us to establish 71 distinct types. Epidemiological data about the relation among P. aeruginosa isolates were not conclusive. The results of the present study indicate that the only method that can establish a clonal relation is DNA restriction enzyme typing, whereas the other methods may cause misleading interpretations and are inadequate to guide proper infection control measures. |
publishDate |
2004 |
dc.date.issued.fl_str_mv |
2004 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:39Z |
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000435655 |
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Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 37, no. 1 (Jan. 2004), p.77-82 |
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