Hide unhairing and characterization of commercial enzymes used in leather manufacture
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/75892 |
Resumo: | The enzymatic treatment of hides in tannery processes is a promising technology. However, the reaction kinetics of commercial enzymes available to the leather industry are not fully understood and their activities have been mainly determined with model proteins such as casein as substrate, which are not of direct relevance for cattle hides. Therefore, it is important to determine their activities on collagen and keratin, the main proteins of skin, in order to use these enzymes in leather processing. This work describes the study of five proteases, used commercially in tanneries, to assess their ability to act upon collagen and keratin and to determine their unhairing. Results showed that all commercial enzymes tested had more activity on collagen than on keratin. Unhairing was also tested and four out of the five enzymes tested showed some unhairing activity. Optima of the temperature and pH of the enzymes were very similar for all five enzymes, with maximal activities around 55°C and pH 9 to 12, respectively. |
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Dettmer, AlineAyub, Marco Antônio ZáchiaGutterres, Mariliz2013-07-16T01:45:08Z20110104-6632http://hdl.handle.net/10183/75892000835201The enzymatic treatment of hides in tannery processes is a promising technology. However, the reaction kinetics of commercial enzymes available to the leather industry are not fully understood and their activities have been mainly determined with model proteins such as casein as substrate, which are not of direct relevance for cattle hides. Therefore, it is important to determine their activities on collagen and keratin, the main proteins of skin, in order to use these enzymes in leather processing. This work describes the study of five proteases, used commercially in tanneries, to assess their ability to act upon collagen and keratin and to determine their unhairing. Results showed that all commercial enzymes tested had more activity on collagen than on keratin. Unhairing was also tested and four out of the five enzymes tested showed some unhairing activity. Optima of the temperature and pH of the enzymes were very similar for all five enzymes, with maximal activities around 55°C and pH 9 to 12, respectively.application/pdfengBrazilian journal of chemical engineering [recurso eletrônico]. São Paulo, SP. Vol. 28, no. 3 (July/Sept. 2011), p. 373-380CouroIndústria do couroEnzimasEnzimaBiotecnologiaUnhairingCommercial enzymesEnzyme characterizationLeather tanningHide unhairing and characterization of commercial enzymes used in leather manufactureinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000835201.pdf.txt000835201.pdf.txtExtracted Texttext/plain28031http://www.lume.ufrgs.br/bitstream/10183/75892/2/000835201.pdf.txtf5cd2a11b41caa6926c952edbd3cbc65MD52ORIGINAL000835201.pdf000835201.pdfTexto completo (inglês)application/pdf970788http://www.lume.ufrgs.br/bitstream/10183/75892/1/000835201.pdfeeeaa1bba68f51aeb2217b1e881e275dMD51THUMBNAIL000835201.pdf.jpg000835201.pdf.jpgGenerated Thumbnailimage/jpeg1988http://www.lume.ufrgs.br/bitstream/10183/75892/3/000835201.pdf.jpg63d4f6de071d2583a0adc1f8619da030MD5310183/758922022-06-03 04:35:43.20302oai:www.lume.ufrgs.br:10183/75892Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-06-03T07:35:43Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Hide unhairing and characterization of commercial enzymes used in leather manufacture |
title |
Hide unhairing and characterization of commercial enzymes used in leather manufacture |
spellingShingle |
Hide unhairing and characterization of commercial enzymes used in leather manufacture Dettmer, Aline Couro Indústria do couro Enzimas Enzima Biotecnologia Unhairing Commercial enzymes Enzyme characterization Leather tanning |
title_short |
Hide unhairing and characterization of commercial enzymes used in leather manufacture |
title_full |
Hide unhairing and characterization of commercial enzymes used in leather manufacture |
title_fullStr |
Hide unhairing and characterization of commercial enzymes used in leather manufacture |
title_full_unstemmed |
Hide unhairing and characterization of commercial enzymes used in leather manufacture |
title_sort |
Hide unhairing and characterization of commercial enzymes used in leather manufacture |
author |
Dettmer, Aline |
author_facet |
Dettmer, Aline Ayub, Marco Antônio Záchia Gutterres, Mariliz |
author_role |
author |
author2 |
Ayub, Marco Antônio Záchia Gutterres, Mariliz |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Dettmer, Aline Ayub, Marco Antônio Záchia Gutterres, Mariliz |
dc.subject.por.fl_str_mv |
Couro Indústria do couro Enzimas Enzima Biotecnologia |
topic |
Couro Indústria do couro Enzimas Enzima Biotecnologia Unhairing Commercial enzymes Enzyme characterization Leather tanning |
dc.subject.eng.fl_str_mv |
Unhairing Commercial enzymes Enzyme characterization Leather tanning |
description |
The enzymatic treatment of hides in tannery processes is a promising technology. However, the reaction kinetics of commercial enzymes available to the leather industry are not fully understood and their activities have been mainly determined with model proteins such as casein as substrate, which are not of direct relevance for cattle hides. Therefore, it is important to determine their activities on collagen and keratin, the main proteins of skin, in order to use these enzymes in leather processing. This work describes the study of five proteases, used commercially in tanneries, to assess their ability to act upon collagen and keratin and to determine their unhairing. Results showed that all commercial enzymes tested had more activity on collagen than on keratin. Unhairing was also tested and four out of the five enzymes tested showed some unhairing activity. Optima of the temperature and pH of the enzymes were very similar for all five enzymes, with maximal activities around 55°C and pH 9 to 12, respectively. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2013-07-16T01:45:08Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/75892 |
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0104-6632 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000835201 |
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0104-6632 000835201 |
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http://hdl.handle.net/10183/75892 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of chemical engineering [recurso eletrônico]. São Paulo, SP. Vol. 28, no. 3 (July/Sept. 2011), p. 373-380 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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