Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21227 |
Resumo: | Diabetes in spontaneously hypertensive rats is associated with cortical renal GLUT1 and GLUT2 overexpression. Our objective was to evaluate the effect of the angiotensin-converting enzyme blockade on cortical renal GLUT1 and GLUT2 expression, urinary albumin and urinary TGF-β1. Streptozotocin, 50 mg/kg, or citrate buffer (N = 16) was administered as a single injection into the tail vein in adult spontaneously hypertensive rats (~260 g). Thirty days later, these diabetic spontaneously hypertensive rats received ramipril by gavage: 0.01 mg·kg-1·day-1 (D0.01, N = 14), 1 mg·kg-1·day-1 (D1, N = 9) or water (D, N = 11) for 15 days. Albumin and TGF-β1 (24-h urine), direct arterial pressure, renal tissue angiotensin-converting enzyme activity (fluorometric assay), and GLUT1 and GLUT2 protein levels (Western blot, renal cortex) were determined. Glycemia and glycosuria were higher (P < 0.05) in the diabetic rats compared with controls, but similar between the diabetic groups. Diabetes in spontaneously hypertensive rats lowered renal tissue angiotensin-converting enzyme activity (40%), which was reduced further when higher ramipril doses were used. Diabetes associated with hypertension raised GLUT1 by 28% (P < 0.0001) and GLUT2 by 76% (P = 0.01), and both doses of ramipril equally reduced cortical GLUT1 (D vs D1 and vs D0.01, P ≤ 0.001). GLUT2 levels were reduced in D0.01 (P < 0.05 vs D). Diabetes increased urinary albumin and TGF-β1 urinary excretion, but the 15-day ramipril treatment (with either dose) did not reduce them. In conclusion, ramipril is effective in lowering renal tissue angiotensin-converting enzyme activity, as well as blocking cortical GLUT1 overexpression, which may be beneficial in arresting the development of diabetic nephropathy. |
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Souza, M. S.Machado, Ubiratan FabresOkamoto, Maristela MitikoBertoluci, Marcello CasacciaPonpermeyer, C.Leguisamo, Natalia MottaAzambuja, FelipeIrigoyen, Maria Claudia CostaSchaan, Beatriz D'Agord2010-04-24T04:15:52Z20080100-879Xhttp://hdl.handle.net/10183/21227000673195Diabetes in spontaneously hypertensive rats is associated with cortical renal GLUT1 and GLUT2 overexpression. Our objective was to evaluate the effect of the angiotensin-converting enzyme blockade on cortical renal GLUT1 and GLUT2 expression, urinary albumin and urinary TGF-β1. Streptozotocin, 50 mg/kg, or citrate buffer (N = 16) was administered as a single injection into the tail vein in adult spontaneously hypertensive rats (~260 g). Thirty days later, these diabetic spontaneously hypertensive rats received ramipril by gavage: 0.01 mg·kg-1·day-1 (D0.01, N = 14), 1 mg·kg-1·day-1 (D1, N = 9) or water (D, N = 11) for 15 days. Albumin and TGF-β1 (24-h urine), direct arterial pressure, renal tissue angiotensin-converting enzyme activity (fluorometric assay), and GLUT1 and GLUT2 protein levels (Western blot, renal cortex) were determined. Glycemia and glycosuria were higher (P < 0.05) in the diabetic rats compared with controls, but similar between the diabetic groups. Diabetes in spontaneously hypertensive rats lowered renal tissue angiotensin-converting enzyme activity (40%), which was reduced further when higher ramipril doses were used. Diabetes associated with hypertension raised GLUT1 by 28% (P < 0.0001) and GLUT2 by 76% (P = 0.01), and both doses of ramipril equally reduced cortical GLUT1 (D vs D1 and vs D0.01, P ≤ 0.001). GLUT2 levels were reduced in D0.01 (P < 0.05 vs D). Diabetes increased urinary albumin and TGF-β1 urinary excretion, but the 15-day ramipril treatment (with either dose) did not reduce them. In conclusion, ramipril is effective in lowering renal tissue angiotensin-converting enzyme activity, as well as blocking cortical GLUT1 overexpression, which may be beneficial in arresting the development of diabetic nephropathy.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. RiBeirão Preto, SP. Vol. 41, n. 11 (Nov. 2008), p. 960-968Inibidores da enzima conversora da angiotensinaDiabetes mellitusHipertensãoAngiotensin-converting enzyme inhibitionDiabetic nephropathyGlucose transporter proteinsHypertensionStreptozotocinReduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive ratsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000673195.pdf.txt000673195.pdf.txtExtracted Texttext/plain38149http://www.lume.ufrgs.br/bitstream/10183/21227/2/000673195.pdf.txtc8a724ce01318f4f79b10d1c3dc15545MD52ORIGINAL000673195.pdf000673195.pdfTexto completo (inglês)application/pdf541110http://www.lume.ufrgs.br/bitstream/10183/21227/1/000673195.pdfadd16e456fd3f59f7ad0f64947a592b0MD51THUMBNAIL000673195.pdf.jpg000673195.pdf.jpgGenerated Thumbnailimage/jpeg2080http://www.lume.ufrgs.br/bitstream/10183/21227/3/000673195.pdf.jpgfada7a4f16bbd333f9f3b210a2d70c62MD5310183/212272018-10-05 08:26:37.14oai:www.lume.ufrgs.br:10183/21227Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-05T11:26:37Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats |
title |
Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats |
spellingShingle |
Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats Souza, M. S. Inibidores da enzima conversora da angiotensina Diabetes mellitus Hipertensão Angiotensin-converting enzyme inhibition Diabetic nephropathy Glucose transporter proteins Hypertension Streptozotocin |
title_short |
Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats |
title_full |
Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats |
title_fullStr |
Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats |
title_full_unstemmed |
Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats |
title_sort |
Reduced cortical renal GLUT1 expression induced by angiotensin-converting enzyme inhibition in diabetic spontaneously hypertensive rats |
author |
Souza, M. S. |
author_facet |
Souza, M. S. Machado, Ubiratan Fabres Okamoto, Maristela Mitiko Bertoluci, Marcello Casaccia Ponpermeyer, C. Leguisamo, Natalia Motta Azambuja, Felipe Irigoyen, Maria Claudia Costa Schaan, Beatriz D'Agord |
author_role |
author |
author2 |
Machado, Ubiratan Fabres Okamoto, Maristela Mitiko Bertoluci, Marcello Casaccia Ponpermeyer, C. Leguisamo, Natalia Motta Azambuja, Felipe Irigoyen, Maria Claudia Costa Schaan, Beatriz D'Agord |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Souza, M. S. Machado, Ubiratan Fabres Okamoto, Maristela Mitiko Bertoluci, Marcello Casaccia Ponpermeyer, C. Leguisamo, Natalia Motta Azambuja, Felipe Irigoyen, Maria Claudia Costa Schaan, Beatriz D'Agord |
dc.subject.por.fl_str_mv |
Inibidores da enzima conversora da angiotensina Diabetes mellitus Hipertensão |
topic |
Inibidores da enzima conversora da angiotensina Diabetes mellitus Hipertensão Angiotensin-converting enzyme inhibition Diabetic nephropathy Glucose transporter proteins Hypertension Streptozotocin |
dc.subject.eng.fl_str_mv |
Angiotensin-converting enzyme inhibition Diabetic nephropathy Glucose transporter proteins Hypertension Streptozotocin |
description |
Diabetes in spontaneously hypertensive rats is associated with cortical renal GLUT1 and GLUT2 overexpression. Our objective was to evaluate the effect of the angiotensin-converting enzyme blockade on cortical renal GLUT1 and GLUT2 expression, urinary albumin and urinary TGF-β1. Streptozotocin, 50 mg/kg, or citrate buffer (N = 16) was administered as a single injection into the tail vein in adult spontaneously hypertensive rats (~260 g). Thirty days later, these diabetic spontaneously hypertensive rats received ramipril by gavage: 0.01 mg·kg-1·day-1 (D0.01, N = 14), 1 mg·kg-1·day-1 (D1, N = 9) or water (D, N = 11) for 15 days. Albumin and TGF-β1 (24-h urine), direct arterial pressure, renal tissue angiotensin-converting enzyme activity (fluorometric assay), and GLUT1 and GLUT2 protein levels (Western blot, renal cortex) were determined. Glycemia and glycosuria were higher (P < 0.05) in the diabetic rats compared with controls, but similar between the diabetic groups. Diabetes in spontaneously hypertensive rats lowered renal tissue angiotensin-converting enzyme activity (40%), which was reduced further when higher ramipril doses were used. Diabetes associated with hypertension raised GLUT1 by 28% (P < 0.0001) and GLUT2 by 76% (P = 0.01), and both doses of ramipril equally reduced cortical GLUT1 (D vs D1 and vs D0.01, P ≤ 0.001). GLUT2 levels were reduced in D0.01 (P < 0.05 vs D). Diabetes increased urinary albumin and TGF-β1 urinary excretion, but the 15-day ramipril treatment (with either dose) did not reduce them. In conclusion, ramipril is effective in lowering renal tissue angiotensin-converting enzyme activity, as well as blocking cortical GLUT1 overexpression, which may be beneficial in arresting the development of diabetic nephropathy. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:52Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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0100-879X |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. RiBeirão Preto, SP. Vol. 41, n. 11 (Nov. 2008), p. 960-968 |
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