Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/267598 |
Resumo: | Microsatellite markers were isolated in Dyckia distachya, an endangered bromeliad from southern Brazil, which will be useful to assess the population genetic structure and reproductive success in introduced and natural populations of this species. Twenty microsatellite loci were developed from an enriched genomic library, and nine of these were amplified. The loci were characterized in 43 individuals from introduced and wild D. distachya populations. All nine loci were polymorphic, with four to ten alleles per locus. In an introduced population the observed and expected heterozygosities ranged from 0.136–0.667 and 0.543–0.877, respectively, while in a wild population it ranged from 0.000 to 0.895 and from 0.050 to 0.811, respectively. The development of these microsatellite markers will contribute to investigations of the reproductive potential and viability of introduced populations of D. distachya as well as the single known wild population. Cross-amplification in other Bromeliaceae species was successful, with high rates in four loci, demonstrating the applicability of these microsatellite markers in other taxa. |
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Zanella, Camila MartiniJanke, AlinePaggi, Gecele MatosGoetze, MárciaReis, Mauricio Sedrez dosBered, Fernanda2023-11-25T03:25:50Z20121422-0067http://hdl.handle.net/10183/267598000931812Microsatellite markers were isolated in Dyckia distachya, an endangered bromeliad from southern Brazil, which will be useful to assess the population genetic structure and reproductive success in introduced and natural populations of this species. Twenty microsatellite loci were developed from an enriched genomic library, and nine of these were amplified. The loci were characterized in 43 individuals from introduced and wild D. distachya populations. All nine loci were polymorphic, with four to ten alleles per locus. In an introduced population the observed and expected heterozygosities ranged from 0.136–0.667 and 0.543–0.877, respectively, while in a wild population it ranged from 0.000 to 0.895 and from 0.050 to 0.811, respectively. The development of these microsatellite markers will contribute to investigations of the reproductive potential and viability of introduced populations of D. distachya as well as the single known wild population. Cross-amplification in other Bromeliaceae species was successful, with high rates in four loci, demonstrating the applicability of these microsatellite markers in other taxa.application/pdfengInternational journal of molecular sciences. Basel. Vol. 13, no. 12 (Nov. 2012), p. 15859-15866MicrossatéliteDickia distachyaEndemismoBromeliadEndemismRheophytic speciesMicrosatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliadsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000931812.pdf.txt000931812.pdf.txtExtracted Texttext/plain20424http://www.lume.ufrgs.br/bitstream/10183/267598/2/000931812.pdf.txta4c8bb89e3dee0d2254c3c4a71d8aac2MD52ORIGINAL000931812.pdfTexto completo (inglês)application/pdf164172http://www.lume.ufrgs.br/bitstream/10183/267598/1/000931812.pdfa7a7a93afa0c684dcd43c3451b4fc410MD5110183/2675982023-12-06 04:24:24.639455oai:www.lume.ufrgs.br:10183/267598Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-12-06T06:24:24Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads |
title |
Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads |
spellingShingle |
Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads Zanella, Camila Martini Microssatélite Dickia distachya Endemismo Bromeliad Endemism Rheophytic species |
title_short |
Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads |
title_full |
Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads |
title_fullStr |
Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads |
title_full_unstemmed |
Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads |
title_sort |
Microsatellites in the endangered species Dyckia distachya (Bromeliaceae) and cross-amplification in other bromeliads |
author |
Zanella, Camila Martini |
author_facet |
Zanella, Camila Martini Janke, Aline Paggi, Gecele Matos Goetze, Márcia Reis, Mauricio Sedrez dos Bered, Fernanda |
author_role |
author |
author2 |
Janke, Aline Paggi, Gecele Matos Goetze, Márcia Reis, Mauricio Sedrez dos Bered, Fernanda |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Zanella, Camila Martini Janke, Aline Paggi, Gecele Matos Goetze, Márcia Reis, Mauricio Sedrez dos Bered, Fernanda |
dc.subject.por.fl_str_mv |
Microssatélite Dickia distachya Endemismo |
topic |
Microssatélite Dickia distachya Endemismo Bromeliad Endemism Rheophytic species |
dc.subject.eng.fl_str_mv |
Bromeliad Endemism Rheophytic species |
description |
Microsatellite markers were isolated in Dyckia distachya, an endangered bromeliad from southern Brazil, which will be useful to assess the population genetic structure and reproductive success in introduced and natural populations of this species. Twenty microsatellite loci were developed from an enriched genomic library, and nine of these were amplified. The loci were characterized in 43 individuals from introduced and wild D. distachya populations. All nine loci were polymorphic, with four to ten alleles per locus. In an introduced population the observed and expected heterozygosities ranged from 0.136–0.667 and 0.543–0.877, respectively, while in a wild population it ranged from 0.000 to 0.895 and from 0.050 to 0.811, respectively. The development of these microsatellite markers will contribute to investigations of the reproductive potential and viability of introduced populations of D. distachya as well as the single known wild population. Cross-amplification in other Bromeliaceae species was successful, with high rates in four loci, demonstrating the applicability of these microsatellite markers in other taxa. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2023-11-25T03:25:50Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/267598 |
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1422-0067 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000931812 |
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url |
http://hdl.handle.net/10183/267598 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
International journal of molecular sciences. Basel. Vol. 13, no. 12 (Nov. 2012), p. 15859-15866 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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