Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model

Detalhes bibliográficos
Autor(a) principal: Corsetti, Adriana
Data de Publicação: 2017
Outros Autores: Bahuschewskyj, Claudia, Ponzoni, Deise, Langie, Renan Cavalheiro, Santos, Luis Alberto dos, Camassola, Melissa, Nardi, Nance Beyer, Puricelli, Edela
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/163933
Resumo: Objectives: this study aimed to evaluate the potential of adupose-derived stem cells (ASCs) combined witha modified tricalcium phospate (a-TCP) or gelatin sponge (GS) scaffolds for bone healing in a rat model. Material and Methods: Bone defects were surgically created in the femur of adult SHR rats and filled with the scaffolds, empty or combined with ASCs. The results were analyzed by histology abd histomorphometry on days seven, 13, 30 and 60. Results: Significantly increased bone repair was observed on days seven and 60 in animals treated with a-TCP/ASCs, and on day 14 in the group trated with GS/ASCs, when compared with the groups treated with the biomaterials alone. Intenses fibroplastia was observed in the group trated with GS alone, on days 14 and 30. Conclusions: Our results showed that the use of ascs combined with a-TCP scaffold suggests osteocunductive property that results in a biological support to the cells, whereas the GS scaffold functions just as a carrier. These results confirm the potential os ASCs in accelerating bone repais in in vivo experimental rat models. These results suggest a new alternative for treating bone defects.
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spelling Corsetti, AdrianaBahuschewskyj, ClaudiaPonzoni, DeiseLangie, Renan CavalheiroSantos, Luis Alberto dosCamassola, MelissaNardi, Nance BeyerPuricelli, Edela2017-07-13T02:37:00Z20171678-7757http://hdl.handle.net/10183/163933001023559Objectives: this study aimed to evaluate the potential of adupose-derived stem cells (ASCs) combined witha modified tricalcium phospate (a-TCP) or gelatin sponge (GS) scaffolds for bone healing in a rat model. Material and Methods: Bone defects were surgically created in the femur of adult SHR rats and filled with the scaffolds, empty or combined with ASCs. The results were analyzed by histology abd histomorphometry on days seven, 13, 30 and 60. Results: Significantly increased bone repair was observed on days seven and 60 in animals treated with a-TCP/ASCs, and on day 14 in the group trated with GS/ASCs, when compared with the groups treated with the biomaterials alone. Intenses fibroplastia was observed in the group trated with GS alone, on days 14 and 30. Conclusions: Our results showed that the use of ascs combined with a-TCP scaffold suggests osteocunductive property that results in a biological support to the cells, whereas the GS scaffold functions just as a carrier. These results confirm the potential os ASCs in accelerating bone repais in in vivo experimental rat models. These results suggest a new alternative for treating bone defects.application/pdfengJournal of applied oral science. Bauru. Vol. 25, no. 1 (Jan./Feb. 2017), p. 10-19Regeneração ósseaCélulas-troncoCálcioBone regenerationCalcium cementAdipose-derived stem cellsRat modelRepair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat modelinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001023559.pdf001023559.pdfTexto completo (inglês)application/pdf777387http://www.lume.ufrgs.br/bitstream/10183/163933/1/001023559.pdfc5e3bf40bb31fcb57fab36b7135236a0MD51TEXT001023559.pdf.txt001023559.pdf.txtExtracted Texttext/plain23316http://www.lume.ufrgs.br/bitstream/10183/163933/2/001023559.pdf.txted4a9d7240950d780967a0c253e2e37fMD52THUMBNAIL001023559.pdf.jpg001023559.pdf.jpgGenerated Thumbnailimage/jpeg1586http://www.lume.ufrgs.br/bitstream/10183/163933/3/001023559.pdf.jpg49bc4dbead468075f92af6db8b3e5e50MD5310183/1639332018-10-10 07:56:52.148oai:www.lume.ufrgs.br:10183/163933Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-10T10:56:52Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model
title Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model
spellingShingle Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model
Corsetti, Adriana
Regeneração óssea
Células-tronco
Cálcio
Bone regeneration
Calcium cement
Adipose-derived stem cells
Rat model
title_short Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model
title_full Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model
title_fullStr Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model
title_full_unstemmed Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model
title_sort Repair of bone defects using adipose-derived stem cells combined with alpha-tricalcium phosphate and gelatin sponge scaffolds in a rat model
author Corsetti, Adriana
author_facet Corsetti, Adriana
Bahuschewskyj, Claudia
Ponzoni, Deise
Langie, Renan Cavalheiro
Santos, Luis Alberto dos
Camassola, Melissa
Nardi, Nance Beyer
Puricelli, Edela
author_role author
author2 Bahuschewskyj, Claudia
Ponzoni, Deise
Langie, Renan Cavalheiro
Santos, Luis Alberto dos
Camassola, Melissa
Nardi, Nance Beyer
Puricelli, Edela
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Corsetti, Adriana
Bahuschewskyj, Claudia
Ponzoni, Deise
Langie, Renan Cavalheiro
Santos, Luis Alberto dos
Camassola, Melissa
Nardi, Nance Beyer
Puricelli, Edela
dc.subject.por.fl_str_mv Regeneração óssea
Células-tronco
Cálcio
topic Regeneração óssea
Células-tronco
Cálcio
Bone regeneration
Calcium cement
Adipose-derived stem cells
Rat model
dc.subject.eng.fl_str_mv Bone regeneration
Calcium cement
Adipose-derived stem cells
Rat model
description Objectives: this study aimed to evaluate the potential of adupose-derived stem cells (ASCs) combined witha modified tricalcium phospate (a-TCP) or gelatin sponge (GS) scaffolds for bone healing in a rat model. Material and Methods: Bone defects were surgically created in the femur of adult SHR rats and filled with the scaffolds, empty or combined with ASCs. The results were analyzed by histology abd histomorphometry on days seven, 13, 30 and 60. Results: Significantly increased bone repair was observed on days seven and 60 in animals treated with a-TCP/ASCs, and on day 14 in the group trated with GS/ASCs, when compared with the groups treated with the biomaterials alone. Intenses fibroplastia was observed in the group trated with GS alone, on days 14 and 30. Conclusions: Our results showed that the use of ascs combined with a-TCP scaffold suggests osteocunductive property that results in a biological support to the cells, whereas the GS scaffold functions just as a carrier. These results confirm the potential os ASCs in accelerating bone repais in in vivo experimental rat models. These results suggest a new alternative for treating bone defects.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-07-13T02:37:00Z
dc.date.issued.fl_str_mv 2017
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/163933
dc.identifier.issn.pt_BR.fl_str_mv 1678-7757
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url http://hdl.handle.net/10183/163933
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Journal of applied oral science. Bauru. Vol. 25, no. 1 (Jan./Feb. 2017), p. 10-19
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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